BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0550.1
         (1631 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

VP13_YEAST (Q07878) Vacuolar protein sorting-associated protein ...   222  7e-57
V13A_HUMAN (Q96RL7) Vacuolar protein sorting 13A (Chorein) (Chor...   214  2e-54
YOS1_SCHPO (P87319) Hypothetical protein C21C3.01c in chromosome II   152  7e-36
VPD2_SCHPO (O42926) Putative vacuolar protein sorting-associated...   137  3e-31
COH1_HUMAN (Q7Z7G8) Cohen syndrome protein 1                           76  7e-13
YM23_YEAST (P40215) Hypothetical 62.8 kDa protein in RPS16A-TIF3...    72  1e-11
NDI1_YEAST (P32340) Rotenone-insensitive NADH-ubiquinone oxidore...    59  1e-07
DHNA_ECOLI (P00393) NADH dehydrogenase (EC 1.6.99.3)                   48  2e-04
DLD2_BACSU (P54533) Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (E3...    42  0.011
YJLD_BACSU (P80861) NADH dehydrogenase-like protein yjlD (EC 1.6...    37  0.58
DLDH_CHLPN (Q9Z773) Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (E3...    37  0.58
DCTP_RHOCA (P37735) C4-dicarboxylate-binding periplasmic protein...    37  0.58
YA82_PASMU (Q9CLW7) Hypothetical protein PM1082                        36  0.99
UVRA_HELPY (P56474) UvrABC system protein A (UvrA protein) (Exci...    35  1.3
SGS1_YEAST (P35187) Helicase SGS1 (Helicase TPS1)                      35  1.7
DHNA_HAEIN (P44856) NADH dehydrogenase (EC 1.6.99.3)                   35  1.7
SYL_TREPA (O83595) Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine-...    35  2.2
GOP1_CAEEL (P46578) Hypothetical protein gop-1                         35  2.2
SELP_HUMAN (P49908) Selenoprotein P precursor (SeP)                    34  3.8
NUC2_SCHPO (P10505) Nuclear scaffold-like protein p76                  34  3.8

>VP13_YEAST (Q07878) Vacuolar protein sorting-associated protein
           VPS13
          Length = 3144

 Score =  222 bits (565), Expect = 7e-57
 Identities = 217/902 (24%), Positives = 385/902 (42%), Gaps = 141/902 (15%)

Query: 1   MLEDQVAYLLQRYLGNYVRGLNKEALKISVWKGDVELKNMQLKPEALNALKLPVKVKAGF 60
           MLE   A LL R LG+YV   +   L + +W GDV+LKN++L+ + L++L LP+ VK+G 
Sbjct: 1   MLESLAANLLNRLLGSYVENFDPNQLNVGIWSGDVKLKNLKLRKDCLDSLNLPIDVKSGI 60

Query: 61  LGSVKLKVPWSRLGQDPVLVYLDRIFLLAEPATQVEGCSEDAVQEA---KKIRIEEMELK 117
           LG + L VPWS L   PV + ++  +LL  P ++     E+ ++ A   K  ++ E EL 
Sbjct: 61  LGDLVLTVPWSSLKNKPVKIIIEDCYLLCSPRSEDHENDEEMIKRAFRLKMRKVSEWELT 120

Query: 118 LWEKSQQLKSEMKTINPSSQ----GSAKKLFSTIIGNLKLSISNIHIRYE--DGESNPGH 171
              +    +SE KT + SS+    G  + L + II NL+++I NIH+RYE  DG    G 
Sbjct: 121 NQARILSTQSENKTSSSSSEKNNAGFMQSLTTKIIDNLQVTIKNIHLRYEDMDGIFTTG- 179

Query: 172 PFAAGVMLDKLSAVTVDDTGKETFITGGALDLIQKLSSMDLFAYQRITKNINSFLNCKMR 231
           P + G+ L++LSAV+ D     +FI                     IT+NI   L     
Sbjct: 180 PSSVGLTLNELSAVSTDSNWAPSFID--------------------ITQNITHKL----- 214

Query: 232 IVKQPLNPKSLSCWSVELDRLAVYLDSDIIPWHASKEWEDLLPSEWFQIFKFGTKDG-KP 290
                          + L+ L +Y ++D  P  +  + +  L +     F  G KD    
Sbjct: 215 ---------------LTLNSLCLYWNTDSPPLISDDDQDRSLEN-----FVRGFKDMIAS 254

Query: 291 ADRLLQKHSYVLEPVTGKGNYSKLLLNEVADS-KQPLQKAVVNLDDVTISLSKDGYRDIM 349
            +    KH Y+L+PV+G G   KL +N++  + +QP     +  D+  + L    Y DI+
Sbjct: 255 KNSTAPKHQYILKPVSGLG---KLSINKLGSTEEQPHIDLQMFYDEFGLELDDTEYNDIL 311

Query: 350 KLADNFAAFNQRLKYAHFRPPVPVKADPRSWWKYAYRAVSDQMKKASGKMSWEQVLRYTS 409
            +  +        K+   RP   V  +P  W+KY    V +++ + +   +WE +     
Sbjct: 312 HVLSSIQLRQITKKFKKARPSFAVSENPTEWFKYIAACVINEIHEKNKMWTWESMKEKCE 371

Query: 410 LRKRYIYLYASLLK--------SDPSQVTISGNKEIEDLDHELDIELILQWRMLAHK--- 458
            R+ Y  L+   LK         DP Q       ++ +L  +L  + I+ +R +A +   
Sbjct: 372 QRRLYTKLWVEKLKLKNLEAPLRDPIQ-----EAQLSELHKDLTYDEIILFRSVAKRQYA 426

Query: 459 -----FVEQSAEPNLSVRKQ---------KAGNSWWSFGWTGKSPKEDSEELSFSEEDWN 504
                  E S  P  S   +         K   SW S  W GK  +E  E+L  +EE   
Sbjct: 427 QYKLGMTEDSPTPTASSNIEPQTSNKSATKNNGSWLSSWWNGKPTEEVDEDLIMTEEQRQ 486

Query: 505 RLNKIIGYKEGDDGQSPVNSKADVMHTFLVVHMNHNASKLIGEAQDL-VAELSCEDLSCS 563
            L   I + E +D    +    + +   +   +   +  +  + Q+L +  +  E+    
Sbjct: 487 ELYDAIEFDENEDKGPVLQVPRERVELRVTSLLKKGSFTIRKKKQNLNLGSIIFENCKVD 546

Query: 564 VKLYPETKVFDIKLGSYQL--SSPKGLL---------AESAASFDS-------------L 599
               P++ +   +L  + L   SP  L          ++  +S D+             L
Sbjct: 547 FAQRPDSFLSSFQLNKFSLEDGSPNALYKHIISVRNSSKDQSSIDNHATGEEEEEDEPLL 606

Query: 600 VGVFKYKPFDDKVDWSMVAKASPCYMTYMKDSINQIVKFFETNATVSQTIALETAAAVQL 659
              F+  P D   D ++  K     + Y    I ++ KFF+ +    +TI      A + 
Sbjct: 607 RASFELNPLDGLADSNLNIKLLGMTVFYHVHFITEVHKFFKASNQHMETIG-NIVNAAEA 665

Query: 660 KIDEVKRTAQQQMNRALKDHARFSLDLDIAAPKITIPTDFYPDNTHATKLLLDLGNLMIR 719
            ++      +  +   L+DH   ++ LD+ AP I +P D  P +      ++D G++ I 
Sbjct: 666 TVEGWTTQTRMGIESLLEDHKTVNVSLDLQAPLIILPLD--PHDWDTPCAIIDAGHMSIL 723

Query: 720 TQ-------DDSRQESAEDN-----------MYLRFDLVLSDVSAFLFDGDYHWSEISVN 761
           +         + ++ S E+            M+ RF ++  D   F+        + ++ 
Sbjct: 724 SDLVPKEKIKEIKELSPEEYDKIDGNEINRLMFDRFQILSQDTQIFVGPD----IQSTIG 779

Query: 762 KLTHSTNTSFFPIIDRCGVILQLQQILLETPY-YPSTRLAVRLPSLAFHFSPARYHRLMH 820
           K+  +++T+ F I+D+  + L +   +L   Y  P+ R+   LP L+   +  +Y  +M+
Sbjct: 780 KINTASSTNDFRILDKMKLELTVDLSILPKAYKLPTIRVFGHLPRLSLSINDIQYKTIMN 839

Query: 821 VI 822
           +I
Sbjct: 840 LI 841


>V13A_HUMAN (Q96RL7) Vacuolar protein sorting 13A (Chorein)
           (Chorea-acanthocytosis protein)
          Length = 3174

 Score =  214 bits (545), Expect = 2e-54
 Identities = 217/870 (24%), Positives = 375/870 (42%), Gaps = 128/870 (14%)

Query: 1   MLEDQVAYLLQRYLGNYVRGLNKEALKISVWKGDVELKNMQLKPEALNALKLPVKVKAGF 60
           + E  V  +L R+LG+YV  L+   L + +WKG V LKN+Q+K  AL+ L +P KVK G 
Sbjct: 2   VFESVVVDVLNRFLGDYVVDLDTSQLSLGIWKGAVALKNLQIKENALSQLDVPFKVKVGH 61

Query: 61  LGSVKLKVPWSRLGQDPVLVYLDRIFLLAEPATQVEGCSEDAVQEAKKI-RIEEMELKLW 119
           +G++KL +PW  L   PV   L+ I+LL  P+++++    D ++E K++   ++ ELK  
Sbjct: 62  IGNLKLIIPWKNLYTQPVEAVLEEIYLLIVPSSRIK---YDPLKEEKQLMEAKQQELKRI 118

Query: 120 EKSQQLKSEMKTINPSSQGS-AKKLFSTIIGNLKLSISNIHIRYEDGESNPGHPFAAGVM 178
           E+++Q   + +   P  Q + A+KL + II NL++ IS+IHIRYED  +N   P + G+ 
Sbjct: 119 EEAKQKVVDQEQHLPEKQDTFAEKLVTQIIKNLQVKISSIHIRYEDDITNRDKPLSFGIS 178

Query: 179 LDKLSAVTVDDTGKETFITGGALDLIQKLSSMD-LFAYQRITKNINSFLNCKMRIVKQPL 237
           L  LS  T D       +      L++KL  +D LFAY                      
Sbjct: 179 LQNLSMQTTDQYWVPC-LHDETEKLVRKLIRLDNLFAY---------------------W 216

Query: 238 NPKSLSCWSVELDRLAVYLDSDIIPWHASKEWEDLLPSEWFQIFKFGTKDGKPADRLLQK 297
           N KS   +  + D     L + I+        E+++P                     + 
Sbjct: 217 NVKSQMFYLSDYDNSLDDLKNGIVN-------ENIVP---------------------EG 248

Query: 298 HSYVLEPVTGKGNYSKLLLNEVA--DSKQPLQKAVVNLDDVTISLSKDGYRDIMKLADNF 355
           + +V  P++     +KL++N  +  D   P     + L ++ I  +K  Y  IM+L ++ 
Sbjct: 249 YDFVFRPISAN---AKLVMNRRSDFDFSAPKINLEIELHNIAIEFNKPQYFSIMELLESV 305

Query: 356 AAFNQRLKYAHFRPPVPVKADPRSWWKYAYRAVSD-QMKKASGKMSWEQVLRYTSLRKRY 414
               Q L Y  F+P VP+    R WW YA   V +  +       SW+ + ++    K+Y
Sbjct: 306 DMMAQNLPYRKFKPDVPLHHHAREWWAYAIHGVLEVNVCPRLWMWSWKHIRKHRQKVKQY 365

Query: 415 IYLYASLL--KSDPSQVTISGNKEIEDLDHELDIELILQWRMLAHKFVEQSAEP--NLSV 470
             LY   L  K  P ++ +S    +E+L+  LD+  I   R  A   V+++        V
Sbjct: 366 KELYKKKLTSKKPPGELLVS----LEELEKTLDVFNITIARQTAEVEVKKAGYKIYKEGV 421

Query: 471 RKQKAGNSW--WSFGWTGKSPKEDSEELS-------FSEEDWNRLNKIIGYKEGDDGQSP 521
           +  +    W  W + W+ ++  E   ++         + E+   L + IGY E      P
Sbjct: 422 KDPEDNKGWFSWLWSWSEQNTNEQQPDVQPETLEEMLTPEEKALLYEAIGYSE--TAVDP 479

Query: 522 VNSKADVMHTF--------LVVHMNHNASKLIGEAQDLVAELSCEDLSCSVKLYPETKV- 572
              K      F        +V+  NH   +L+        ++  E+ S  +   P  +  
Sbjct: 480 TLLKTFEALKFFVHLKSMSIVLRENHQKPELV--------DIVIEEFSTLIVQRPGAQAI 531

Query: 573 -FDIKLGSYQL-----SSPKGLLAESAASFDSLVGV-FKYKPFDDKVDWSMVAKASPCYM 625
            F+ K+ S+ +     +S K  L  S     SL  + F+  P D+ V    + +A P  +
Sbjct: 532 KFETKIDSFHITGLPDNSEKPRLLSSLDDAMSLFQITFEINPLDETVSQRCIIEAEPLEI 591

Query: 626 TYMKDSINQIVKFFETNATVSQTIALETAAAVQLKIDEVKRTAQQQMNRALKDHARFSLD 685
            Y   ++N IV+FF     V      +  AA   K++E +      +   ++      L 
Sbjct: 592 IYDARTVNSIVEFFRPPKEVH---LAQLTAATLTKLEEFRSKTATGLLYIIETQKVLDLK 648

Query: 686 LDIAAPKITIPTD--FYPDNTHATKLLLDLGNLMIRT----------QDDSRQESAEDNM 733
           +++ A  I +P D  F P    +  LLLDLG+L + +          Q ++  +   D  
Sbjct: 649 INLKASYIIVPQDGIFSPT---SNLLLLDLGHLKVTSKSRSELPDVKQGEANLKEIMDRA 705

Query: 734 YLRFDLVLSDVSAFLFDGDYHWSEISVNKLTHSTNTSFFPIIDRCGVILQLQQILLETPY 793
           Y  FD+ L+ V         +W E    KL+ ST     P+     + L    + ++   
Sbjct: 706 YDSFDIQLTSVQLLYSRVGDNWRE--ARKLSVSTQHILVPM--HFNLELSKAMVFMDV-R 760

Query: 794 YPSTRLAVRLPSLAFHFSPARYHRLMHVIK 823
            P  ++  +LP ++   S  +   +M +I+
Sbjct: 761 MPKFKIYGKLPLISLRISDKKLQGIMELIE 790



 Score = 53.5 bits (127), Expect = 5e-06
 Identities = 94/493 (19%), Positives = 217/493 (43%), Gaps = 42/493 (8%)

Query: 1148 STKEHELNDTLE-SFVKAQIIIYDQNSTRYNNIDKQVIVTLATLTFFCRRPTILAIMEFI 1206
            +T ++ + D L   +VKA+  + D  ST YNN+ + + V  ++L        +L  + ++
Sbjct: 952  TTLDNTMEDLLTLEYVKAEKNVPDLKST-YNNVLQLIKVNFSSLDIHLHTEALLNTINYL 1010

Query: 1207 NSINIENGNLATSSDSSSTSMVKNDLSNDLDVLHVTTVEEHAVKGLLGKGKSRVMFSLTL 1266
            +       N+   S+  S  +   +  +  DV     +++ A+K  L   +  +   +  
Sbjct: 1011 H-------NILPQSEEKSAPVSTTETEDKGDV-----IKKLALK--LSTNEDIITLQILA 1056

Query: 1267 KMAQAQILLMKENETKLACLSQESLLAEIKVFPSSFSIKAALGNLKISDDSLSSSHFYYW 1326
            +++  QI + ++ +  ++ +  E L +E+ + PS   I A L N+ + D  +++   Y  
Sbjct: 1057 ELSCLQIFI-QDQKCNISEIKIEGLDSEMIMRPSETEINAKLRNIIVLDSDITA--IYKK 1113

Query: 1327 ACDMRNPGGRSFVELEFTSFSCDDE--DYEGYDFSLFGELSEVRIVYLNRFLQEIVGYFM 1384
            A  +      SF  + +   +      D    D  +   +  + +V++ +FL  I+  F+
Sbjct: 1114 AVYITGKEVFSFKMVSYMDATAGSAYTDMNVVDIQVNLIVGCIEVVFVTKFLYSILA-FI 1172

Query: 1385 GLVPNSPRSVVKVTDQVTNSEKWFSASDIEGSPAVKFDLSLRKPIILMPRNRDSLDFLRL 1444
                 + +++ + T Q             + S  +  D++++ P++++P++  S +    
Sbjct: 1173 DNFQAAKQALAEATVQAAGMAATGVRELAQRSSRMALDINIKAPVVVIPQSPVSENVFVA 1232

Query: 1445 DIVHITVRNTFQWIGGSKSEINAVHLETLMVQVEDINLNVGTGTDLGESIIKDV---NGL 1501
            D   IT+ NTF  I  S+S    V ++ + +++ ++ L      +     + D+     L
Sbjct: 1233 DFGLITMTNTFHMITESQSSPPPV-IDLITIKLSEMRLYRSRFINDAYQEVLDLLLPLNL 1291

Query: 1502 SVIIHRSL-RDLSHQFPSIEIIIKMEELKAAMSNKEYQIITECAVSN-FSEVPDIPSPL- 1558
             V++ R+L  +   + P   +  +++ ++  +S ++   I +    N + E     SP  
Sbjct: 1292 EVVVERNLCWEWYQEVPCFNVNAQLKPMEFILSQEDITTIFKTLHGNIWYEKDGSASPAV 1351

Query: 1559 --NQYSSKTLNGATDDIVPEVTSGADSVTTDV----EASVLLKIWVSINL----VELSLY 1608
              +QYS+   +G T +     + GA  VT  V      ++L+K  ++I+     + + LY
Sbjct: 1352 TKDQYSA--TSGVTTN-ASHHSGGATVVTAAVVEVHSRALLVKTTLNISFKTDDLTMVLY 1408

Query: 1609 TGISRDASLATVQ 1621
            +   + AS   V+
Sbjct: 1409 SPGPKQASFTDVR 1421


>YOS1_SCHPO (P87319) Hypothetical protein C21C3.01c in chromosome II
          Length = 3011

 Score =  152 bits (384), Expect = 7e-36
 Identities = 167/763 (21%), Positives = 325/763 (41%), Gaps = 102/763 (13%)

Query: 1   MLEDQVAYLLQRYLGNYVRGLNKEALKISVWKGDVELKNMQLKPEALNALKLPVKVKAGF 60
           MLE  VA LL + LG+YV  L+ + L I VW G V L N+++KPEAL+ L +P+++ +G 
Sbjct: 1   MLEGLVAGLLNKILGSYVDNLDTKQLNIGVWGGHVSLHNLRIKPEALDKLGIPIEITSGL 60

Query: 61  LGSVKLKVPWSRLGQDPVLVYLDRIFLLAEPATQVEGCSEDAVQEAKKIRIEEME-LKLW 119
           +G+  L++PWS L    + + ++ I+L   P  +     ++  Q  + ++ E+++  ++ 
Sbjct: 61  IGTFTLEIPWSNLRNKSLTINIEDIYLSIHPQAKNSLTRDELEQSQQALKQEQLDSFEIL 120

Query: 120 EKSQQLKSEMKTINPS---SQGSAKKLFSTIIGNLKLSISNIHIRYEDGESNPGHPFAAG 176
            K+ +   E  + NP+    Q   + L + +  N+++ I  IH+R+ED  S+   P++ G
Sbjct: 121 RKNFRETLEESSSNPNISRKQSFIEYLIAKLTDNIQIYIERIHLRFEDNLSDLEKPYSLG 180

Query: 177 VMLDKLSAVTVDDTGKETFITGGAL--DLIQKLSSMDLFAYQRITKNINSFLNCKMRIVK 234
           + L  L   + D +  E  ++   +    I K+ ++D F+   I+K       C++    
Sbjct: 181 LTLYSLRVTSTDASFTEYLLSTDPIPSSCIHKIITVDYFSIYWISK-------CEIS--- 230

Query: 235 QPLNPKSLSCWSVELDRLAVYLDSDIIPWHASKEWEDLLPSEWFQIFKFGTKDGKPADRL 294
                    C + E          DI  +      ++L+PS           +  PA   
Sbjct: 231 --------KCTTTE----------DIFSY-----LKNLIPS----------AEKSPA--- 254

Query: 295 LQKHSYVLEPVTGKGNYSKLLLNEVADSKQPLQKAVVNLDDVTISLSKDGYRDIMKLADN 354
              ++Y+L+P+    +   +L     D    L +  +++++++I+LS   Y  ++ + D 
Sbjct: 255 ---YNYILKPLRATAHV--VLFRHPTDQIMQL-RGKLSVEEISITLSDHMYYSLLGVIDY 308

Query: 355 FAAFNQRLKYAHFRPPVPVKADPRSWWKYAYRAVSDQMKKASGKMSWEQVLRYTSLRKRY 414
           F    ++  Y  +RP    K  P  W+KYA   V D + ++    +W+   R    R  Y
Sbjct: 309 FRVVMKQQYYLQYRPKSTPKEKPLEWFKYAILVVKDSVHESRYHWTWKYFKRRRDDRIAY 368

Query: 415 IYLYAS-LLKSDPSQVTISGNKEIEDLDHELDIELILQWRMLAH-KFVEQSAEPNLSVRK 472
           +++     L    S+  I   K+IE  +   D   ++++R   H   +E+     L  + 
Sbjct: 369 MHIIRKRYLNEQISKEEIDLQKKIEKRNSTYD---LIKYRSRVHTSLIEERNSIYLKPKT 425

Query: 473 QKAGNSW-WSFGWTGKSPKEDSEELS-----------------FSEEDWNRLNKIIGYKE 514
             A   + W  G+  K   +D + L+                 FS  +W+          
Sbjct: 426 SAAHGLYDWFSGYIRKPQSQDEDTLASTDKTAADLTDQEQKEFFSAIEWSG-QLYPDTVN 484

Query: 515 GDDGQSPVNSKADVMHTFLVV--HMNHNASKLIGEAQDLVAELSCEDLSCSVKLYPETKV 572
            D      N +  +     V+  H+N     LI +  +  A   C     S+KL    K 
Sbjct: 485 LDPDMCMANVEVSIAKGSFVIQSHINGRVIPLIKQRFESFA-TECFIRPQSLKLKVSLKD 543

Query: 573 FDIKLG--SYQLSSPKGLLA----ESAASFDSLVGVFKYKPFDDKVDWSMVAKASPCYMT 626
            D+  G  + +L   + + A    E + S   +   ++   F   +D   V KAS   + 
Sbjct: 544 LDMFDGITNPELEPARVIFAKPSVEESESLQKIPEAYRTHLFFLLLDTKPVYKASSTLIV 603

Query: 627 YMKD--------SINQIVKFFETNATVSQTIALETAAAVQLKIDEVKRTAQQQMNRALKD 678
           +++          I  ++ FF    T  + ++ E   +   K+  + R  +  ++ AL+ 
Sbjct: 604 HLRTLVIIYNRVCIESLLAFFVPPRTKIEHVS-EWGYSAAAKVMTLARQTRASLDYALEM 662

Query: 679 HARFSLDLDIAAPKITIPTDFYPDNTHATKLLLDLGNLMIRTQ 721
           H    + +D+ AP I +  +    +  +  L LD+G  ++ TQ
Sbjct: 663 HKTSDMTIDLQAPLIVVREEC--TDLKSPTLFLDVGRALVHTQ 703


>VPD2_SCHPO (O42926) Putative vacuolar protein sorting-associated
           protein vps1302
          Length = 3131

 Score =  137 bits (344), Expect = 3e-31
 Identities = 197/875 (22%), Positives = 364/875 (41%), Gaps = 110/875 (12%)

Query: 1   MLEDQVAYLLQRYLGNYVRGLNKEALKISVWKGDVELKNMQLKPEALNALKLPVKVKAGF 60
           MLE  +A  L R LG Y+   +   LK++VW GDV L+N+QLK  A   L+LP+ V+ G 
Sbjct: 1   MLEGLLANFLNRLLGEYIENFDATQLKVAVWNGDVTLRNLQLKRSAFRKLELPISVQYGL 60

Query: 61  LGSVKLKVPWSRLGQDPVLVYLDRIFLLAEPATQVEGCSEDAVQEAKKIRIEEMELKLWE 120
           +  + LK+PWS L   PV +Y+  I  LA     V+  SE  V   + +  +  +++LWE
Sbjct: 61  VEELTLKIPWSSLKNKPVEIYIVGIRALASMEENVKSQSE--VDPHQVLESKRRQMQLWE 118

Query: 121 KSQQLKSEMKTINPSSQGSAKKLFSTIIGNLKLSISNIHIRYED---GESNPGHPFAAGV 177
            SQ  K+E    +P +Q   + L + +I N++++I +IHIR+E        PG  ++ G+
Sbjct: 119 ASQIGKAE-TAYDPKTQTFTESLITRMIDNIQINIRDIHIRFEHLPVSNVVPG-GYSFGL 176

Query: 178 MLDKLSAVTVDDTGKETFITGGALDLIQKLSSMDLFAYQRITKNINSFLNCKMRIVKQPL 237
           +L + S  + ++    TF+                      T++   +  C         
Sbjct: 177 LLSEFSIESCNEEWTSTFVE---------------------TESQTIYKLC--------- 206

Query: 238 NPKSLSCWSVELDRLAVYLDSDIIPWHASKEWEDLLPSEWFQIFKFGTKDGKPADRLLQK 297
              SL  + +  D  A  +D   I             +E    F+    D +  ++    
Sbjct: 207 ---SLRGFGIYSDETAECIDKTDI-------------NELMNTFQALITDFQSREK---- 246

Query: 298 HSYVLEPVTGKGNYSKLLLNEVADSKQPLQKAVVNLDDVTISLSKDGYRDIMKLADNFAA 357
             Y++ PVTG    +K+ +N++   + P   + ++     +SL+       M LA     
Sbjct: 247 -DYIIAPVTG---MAKVTINKLPTPEIPRFLSQISFRGFDVSLNDSQISCGMSLAHELQD 302

Query: 358 FNQRLKYAHFRPPVPVKAD-PRSWWKYAYRAVSDQMKKASGKMSWEQVLRYTSLRKRYIY 416
              +L +   R    V  D P  + ++ ++     +++     SW  +  +   R+ YI 
Sbjct: 303 VMSKLAFRK-RIVGNVSLDTPLDYLRFIFQKTLHDIQQKHYARSWPAIKAFCEKRRNYIK 361

Query: 417 LYASLLKSDPSQVTISGNKEIEDLDHELDIELILQWRMLAHKFVE-QSAEPNLSVRKQKA 475
           LY    K    Q++   +KE++ L+  LDI  +  +R LA++ ++ +  EP     KQ+ 
Sbjct: 362 LYKK--KFLAVQLSADESKELDVLELNLDISQLKLFRSLAYQEIKNEGFEP--QPPKQQG 417

Query: 476 GNSWWSFGWTGKSPK--EDSEELSFSEEDWNRLN-KIIGYKEGDDGQSPVNSKADV---- 528
             SW    + G S +  E +++   +  D   LN  II      D QS   S  DV    
Sbjct: 418 WGSWMWNSFRGASDENGEVTDDQRKTVIDAIGLNDSIIESAHVGDLQSN-TSLFDVRLEV 476

Query: 529 -MHTFLVVHMNHNASKLIGEAQDLVAELSC--EDLSC-----SVKLYPETKVFDIKLGSY 580
               F ++      + +  E  +  ++  C  +D S      S+K+Y + + F      Y
Sbjct: 477 PSGKFSLLKYPKKENVISLELLNFFSQFKCNKKDYSFVANLGSLKMYNDDRNF-----LY 531

Query: 581 QLSSPKGLLAESAASFDSLVGVFKYKPFDDKVDWSMVAKASPCYMTYMKDSINQIVKFFE 640
              +    +  ++ S  +L   F+    DD  D   +         +   ++   VK F+
Sbjct: 532 PRETSNKEIETTSPSIFTL--SFERSSSDDN-DTDTLGINLRALEFFYDPNLLLRVKGFQ 588

Query: 641 TNATVSQTIALETAAAVQLKIDEVKRTAQQQMNRALKDHARFSLDLDIAAPKITIPTDFY 700
           ++   +         A     D   +T Q  +   L +  + +++     P +  P D  
Sbjct: 589 SSLFANDNGRKLVRLANDAVTDFTSQTVQ-NLQEYLNEKRKLNVNFQFQTPLLIFPEDC- 646

Query: 701 PDNTHATKLLLDLGNLMIRTQ----DDSRQESAEDNMY-LRFDLV-LSDVSAFLFDGDYH 754
            +N  +  L +D G + I +Q    +D    S  + +Y L +D   +S  SA L  G  +
Sbjct: 647 -NNPESFSLFVDAGFISITSQNVETNDKESNSESETLYHLIYDRYRVSLESARLLLGPLN 705

Query: 755 WSEISVNKLTHSTNTSFFPIIDRCGVILQLQQILLETPYYPSTRLAVRLPSLAFHFSPAR 814
             +   NK+        + +++     + +Q   + TP YP  ++   +P L+F  S A+
Sbjct: 706 ELKNESNKINEE-----YYLMNELNTEIFIQAQRVPTPQYPRIKVEGNMPCLSFILSDAQ 760

Query: 815 YHRLMH----VIKIFEEGDDGSSEFLRPWNQADLE 845
           +  L +    ++K  +  DD  +   RP +   L+
Sbjct: 761 FRILNNLASTMLKDGKASDDDDNGDWRPESSESLD 795


>COH1_HUMAN (Q7Z7G8) Cohen syndrome protein 1
          Length = 4022

 Score = 76.3 bits (186), Expect = 7e-13
 Identities = 48/205 (23%), Positives = 95/205 (45%), Gaps = 8/205 (3%)

Query: 1   MLEDQVAYLLQRYLGNYVRGLNKEALKISVWKGDVELKNMQLKPEAL-NALKLPVKVKAG 59
           MLE  V  +L  Y+  Y++ L    L++S+W GDV L  ++LK + L   LKLP    +G
Sbjct: 1   MLESYVTPILMSYVNRYIKNLKPSDLQLSLWGGDVVLSKLELKLDVLEQELKLPFTFLSG 60

Query: 60  FLGSVKLKVPWSRLGQDPVLVYLDRIFLLAEPATQVEGCSEDAVQEAKKIRIEEMELKLW 119
            +  +++ VPW++LG +PV++ ++ +  + +    ++   E     +   R      K  
Sbjct: 61  HIHELRIHVPWTKLGSEPVVITINTMECILKLKDGIQDDHESCGSNSTN-RSTAESTKSS 119

Query: 120 EKSQQLKSEMKTINPSSQGSAKKLFSTIIGNLKLSISNIHIRYEDGESNPGHPFAAGVML 179
            K ++++    T      G  + L   ++ N+ + I+N+ ++Y + +          V +
Sbjct: 120 IKPRRMQQAAPTDPDLPPGYVQSLIRRVVNNVNIVINNLILKYVEDD------IVLSVNI 173

Query: 180 DKLSAVTVDDTGKETFITGGALDLI 204
                 TV +     F+   A DL+
Sbjct: 174 TSAECYTVGELWDRAFMDISATDLV 198


>YM23_YEAST (P40215) Hypothetical 62.8 kDa protein in RPS16A-TIF34
           intergenic region
          Length = 560

 Score = 72.0 bits (175), Expect = 1e-11
 Identities = 36/84 (42%), Positives = 57/84 (67%), Gaps = 3/84 (3%)

Query: 856 MVSFICASLTIGGPTGVEFAASLHDFVNEDLVRLYPGIKDIVKITLLEAGDHILSMFDKR 915
           ++SF+      GGPTGVEFAA L D+V++DL +  P +   +K+TL+EA  +IL+MFDK 
Sbjct: 274 LLSFVVVG---GGPTGVEFAAELRDYVDQDLRKWMPELSKEIKVTLVEALPNILNMFDKY 330

Query: 916 ITAFADDKFQRDGIDVKTGSVVVK 939
           +  +A D F+ + ID++  ++V K
Sbjct: 331 LVDYAQDLFKEEKIDLRLKTMVKK 354


>NDI1_YEAST (P32340) Rotenone-insensitive NADH-ubiquinone
           oxidoreductase, mitochondrial precursor (EC 1.6.5.3)
           (Internal NADH dehydrogenase)
          Length = 513

 Score = 58.9 bits (141), Expect = 1e-07
 Identities = 28/73 (38%), Positives = 46/73 (62%)

Query: 867 GGPTGVEFAASLHDFVNEDLVRLYPGIKDIVKITLLEAGDHILSMFDKRITAFADDKFQR 926
           GGPTGVE A  L D+V++DL +  P + + V+I L+EA   +L+MF+K+++++A    + 
Sbjct: 236 GGPTGVEAAGELQDYVHQDLRKFLPALAEEVQIHLVEALPIVLNMFEKKLSSYAQSHLEN 295

Query: 927 DGIDVKTGSVVVK 939
             I V   + V K
Sbjct: 296 TSIKVHLRTAVAK 308


>DHNA_ECOLI (P00393) NADH dehydrogenase (EC 1.6.99.3)
          Length = 433

 Score = 48.1 bits (113), Expect = 2e-04
 Identities = 33/96 (34%), Positives = 55/96 (56%), Gaps = 5/96 (5%)

Query: 867 GGPTGVEFAASLHDFVNEDLVRLYPGI-KDIVKITLLEAGDHILSMFDKRITAFADDKFQ 925
           GG TGVE +A LH+ V +     Y G+  + + +TL+EAG+ IL     RI+A A ++  
Sbjct: 177 GGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELT 236

Query: 926 RDGIDVKTGSVVVKSTDPISGLSETSSDHDDIESEL 961
           + G+ V T ++V  + +   GL   + D + IE++L
Sbjct: 237 KLGVRVLTQTMVTSADE--GGLH--TKDGEYIEADL 268


>DLD2_BACSU (P54533) Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (E3
           component of branched-chain alpha-keto acid
           dehydrogenase complex) (LPD-Val) (Dihydrolipoamide
           dehydrogenase)
          Length = 474

 Score = 42.4 bits (98), Expect = 0.011
 Identities = 32/112 (28%), Positives = 53/112 (46%), Gaps = 22/112 (19%)

Query: 867 GGPTGVEFAASLHDFVNEDLVRLYPGIKDIVKITLLEAGDHILSMFDKRITAFADDKFQR 926
           GG  G+E+A+ LHDF               VK+T++E  D IL   D  I+   +   ++
Sbjct: 189 GGVIGIEWASMLHDFG--------------VKVTVIEYADRILPTEDLEISKEMESLLKK 234

Query: 927 DGIDVKTGSVVVKSTDPISGLSETSSDHDDIESELDNQGVIDVAISERLFVT 978
            GI   TG+ V+  T     +++TS   DDI  + +  G      +E++ V+
Sbjct: 235 KGIQFITGAKVLPDT-----MTKTS---DDISIQAEKDGETVTYSAEKMLVS 278


>YJLD_BACSU (P80861) NADH dehydrogenase-like protein yjlD (EC
           1.6.99.-) (Glucose starvation-inducible protein 5)
           (GSI5)
          Length = 391

 Score = 36.6 bits (83), Expect = 0.58
 Identities = 33/131 (25%), Positives = 55/131 (41%), Gaps = 21/131 (16%)

Query: 823 KIFEEGDDGSSEFLRPWNQADLEGWLSLLTWKVMVSFICASLTIGGP--TGVEFAASLHD 880
           K+F+  +D   E+ +  N+AD                  A++ IGG   TGVE    L D
Sbjct: 133 KVFQHVEDRVREYSKTKNEAD------------------ATILIGGGGLTGVELVGELAD 174

Query: 881 FVNEDLVRLYPGIKDIVKITLLEAGDHILSMFDKRITAFADDKFQRDGIDVKTGSVVVKS 940
            +  +L + Y      +K+ L+EAG  IL +    +   A    ++ G++  TG  V   
Sbjct: 175 IM-PNLAKKYGVDHKEIKLKLVEAGPKILPVLPDDLIERATASLEKRGVEFLTGLPVTNV 233

Query: 941 TDPISGLSETS 951
              +  L + S
Sbjct: 234 EGNVIDLKDGS 244


>DLDH_CHLPN (Q9Z773) Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (E3
           component of 2-oxoglutarate dehydrogenase complex)
           (Dihydrolipoamide dehydrogenase)
          Length = 461

 Score = 36.6 bits (83), Expect = 0.58
 Identities = 22/67 (32%), Positives = 34/67 (49%), Gaps = 14/67 (20%)

Query: 867 GGPTGVEFAASLHDFVNEDLVRLYPGIKDIVKITLLEAGDHILSMFDKRITAFADDKFQR 926
           GG  G EFA+  H                 V+IT++EA DHIL++ +K ++    +KF +
Sbjct: 178 GGVIGCEFASLFHTLG--------------VEITVIEALDHILAVNNKEVSQTVTNKFTK 223

Query: 927 DGIDVKT 933
            GI + T
Sbjct: 224 QGIRILT 230


>DCTP_RHOCA (P37735) C4-dicarboxylate-binding periplasmic protein
           precursor
          Length = 333

 Score = 36.6 bits (83), Expect = 0.58
 Identities = 37/131 (28%), Positives = 59/131 (44%), Gaps = 14/131 (10%)

Query: 857 VSFICASLTIGGPTGVEFAASLHDFVNEDLVRLYPGIKDIVKITLLEAGDHILSMFD-KR 915
           V  +  SL   GP GV+      DF   DL  ++   + + K+T  EAG  +LS  + K 
Sbjct: 87  VQMLAPSLAKFGPLGVQ------DFEVFDLPYIFKDYEALHKVTQGEAGKMLLSKLEAKG 140

Query: 916 ITAFADDKFQRDGIDVKTGSVVVKSTDPISGLSETSSDHDDIESELDNQGVID--VAISE 973
           IT  A   F  +G  + + +  +   D   GL         +E+E++  G +   +A SE
Sbjct: 141 ITGLA---FWDNGFKIMSANTPLTMPDDFLGLKMRIQSSKVLEAEMNALGAVPQVMAFSE 197

Query: 974 --RLFVTGVLD 982
             +   TGV+D
Sbjct: 198 VYQALQTGVVD 208


>YA82_PASMU (Q9CLW7) Hypothetical protein PM1082
          Length = 343

 Score = 35.8 bits (81), Expect = 0.99
 Identities = 24/80 (30%), Positives = 41/80 (51%), Gaps = 10/80 (12%)

Query: 831 GSSEFLRPWNQADLEGWLSLLTWKVMVSFICASLTIGGPTGVEFAASLHDFV-------N 883
           G+S+ L P  +AD + +L LL  +  V F+C  L I  P  ++   +LHDF         
Sbjct: 150 GNSQLLDPNCEAD-QAYLQLL--EEAVDFMCEKLDIDAPEPIDITEALHDFTTLLNIHDK 206

Query: 884 EDLVRLYPGIKDIVKITLLE 903
           E+ ++ Y  I++  +  LL+
Sbjct: 207 EEFIKRYQQIQETPEKCLLD 226


>UVRA_HELPY (P56474) UvrABC system protein A (UvrA protein)
            (Excinuclease ABC subunit A)
          Length = 935

 Score = 35.4 bits (80), Expect = 1.3
 Identities = 27/92 (29%), Positives = 40/92 (43%), Gaps = 6/92 (6%)

Query: 939  KSTDPISGLSETSSDHDDIESELDNQGVIDVAISERLFVTGVLDELKVCFSYSYQPDQSL 998
            K T  ++G+     +H D    LD   +     S     TGV+DE+++ F+     +Q  
Sbjct: 657  KKTQSLNGVEIVGLEHLDKVIYLDQAPIGKTPRSNPATYTGVMDEIRILFA-----EQKE 711

Query: 999  MKVLLNQEKRLFEFRAIGGQVEVSMKDNDIFI 1030
             K+L     R F F   GG+ E    D DI I
Sbjct: 712  AKILGYSASR-FSFNVKGGRCEKCQGDGDIKI 742


>SGS1_YEAST (P35187) Helicase SGS1 (Helicase TPS1)
          Length = 1447

 Score = 35.0 bits (79), Expect = 1.7
 Identities = 43/189 (22%), Positives = 78/189 (40%), Gaps = 12/189 (6%)

Query: 1131 PKFSRIIGLLPTDAPS-TSTKEHELNDTLESFVKAQIIIYDQNSTRYNNIDKQVIVTLAT 1189
            P  +  I  +P   P+ T+TK+HE+  TL +    + + Y   S +Y ++    I    +
Sbjct: 58   PGTTNFITSIPASGPTNTATKQHEVMQTLSN--DTEWLSYTATSNQYADVPMVDIPASTS 115

Query: 1190 LTFFCRRPTILAIMEFINSINIENGNLATSSDSSSTSMVKNDLSNDLDVLHVTTVEEHAV 1249
            +    R P       F N+      +    +DSS     +N+  + +D   +    E+ +
Sbjct: 116  VVSNPRTPNGSKTHNF-NTFRPHMASSLVENDSSRNLGSRNNNKSVIDNSSIGKQLENDI 174

Query: 1250 KGLLGKGKSRVMFSLTLKMAQAQILLMKENETKLACLSQESLLAEIKVFPSSFSIKAALG 1309
            K        R+  SL + + +   LL++    K + +   SL  + K    S  I+  L 
Sbjct: 175  K----LEVIRLQGSLIMALKEQSKLLLQ----KCSIIESTSLSEDAKRLQLSRDIRPQLS 226

Query: 1310 NLKISDDSL 1318
            N+ I  DSL
Sbjct: 227  NMSIRIDSL 235


>DHNA_HAEIN (P44856) NADH dehydrogenase (EC 1.6.99.3)
          Length = 444

 Score = 35.0 bits (79), Expect = 1.7
 Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 3/76 (3%)

Query: 867 GGPTGVEFAASLHDFVNEDLVRLYPGIKD--IVKITLLEAGDHILSMFDKRITAFADDKF 924
           GG TGVE  A L+    EDL     G  D   +++TL+EAG  +L    + ++A   D+ 
Sbjct: 188 GGATGVELTAELYHAA-EDLSSYGYGKIDSSCLQVTLVEAGTRLLPALPENLSAAVLDEL 246

Query: 925 QRDGIDVKTGSVVVKS 940
           +  G +V+  +++ ++
Sbjct: 247 KEMGTNVQLNTMITEA 262


>SYL_TREPA (O83595) Leucyl-tRNA synthetase (EC 6.1.1.4)
           (Leucine--tRNA ligase) (LeuRS)
          Length = 878

 Score = 34.7 bits (78), Expect = 2.2
 Identities = 25/92 (27%), Positives = 41/92 (44%), Gaps = 1/92 (1%)

Query: 15  GNYVRGLNKEALKISVWKGDVELKNMQLKPEALNALKLPV-KVKAGFLGSVKLKVPWSRL 73
           G YV     E L+  V K    LKN+    + + A      +V   F+G ++   PW+  
Sbjct: 617 GTYVHARTGEKLECVVAKMSKALKNVVNPDDMIAAYGADACRVYEMFMGPLEASKPWNTQ 676

Query: 74  GQDPVLVYLDRIFLLAEPATQVEGCSEDAVQE 105
           G   V  +L++I++LA       G  +D+  E
Sbjct: 677 GLVGVFRFLEKIWVLAGRVAAANGIPQDSRAE 708


>GOP1_CAEEL (P46578) Hypothetical protein gop-1
          Length = 892

 Score = 34.7 bits (78), Expect = 2.2
 Identities = 40/151 (26%), Positives = 61/151 (39%), Gaps = 29/151 (19%)

Query: 1080 GLIPTESDDKFYEAPE--TLAD--SDVYMQSPGGTSEYPSSSSNEIKFNYSSLEPPKFSR 1135
            G+  TE D+ F++ PE  TL D   DV + +       P         N  S    +F  
Sbjct: 450  GVEATEEDEIFHDVPEEQTLEDLVDDVLVDTENSAISDPEPK------NVESESRSRFQS 503

Query: 1136 IIGLLPTDAPSTSTKEHELNDTLESFVKAQIIIYDQNSTRYNNI---------------D 1180
             +  LP   PSTS  +  L D L S +KA  +  D N  R   +               D
Sbjct: 504  AVDELPP--PSTSGCDGRLFDALSSIIKA--VGTDDNRIRPITLELACLVIRQILMTVDD 559

Query: 1181 KQVIVTLATLTFFCRRPTILAIMEFINSINI 1211
            ++V  +L  L F  R   + +I +++N  N+
Sbjct: 560  EKVHTSLTKLCFEVRLKLLSSIGQYVNGENL 590


>SELP_HUMAN (P49908) Selenoprotein P precursor (SeP)
          Length = 381

 Score = 33.9 bits (76), Expect = 3.8
 Identities = 14/39 (35%), Positives = 22/39 (55%)

Query: 333 LDDVTISLSKDGYRDIMKLADNFAAFNQRLKYAHFRPPV 371
           L+D+ + L K+GY +I  +  N    + RLKY H +  V
Sbjct: 69  LEDLRVKLKKEGYSNISYIVVNHQGISSRLKYTHLKNKV 107


>NUC2_SCHPO (P10505) Nuclear scaffold-like protein p76
          Length = 665

 Score = 33.9 bits (76), Expect = 3.8
 Identities = 19/65 (29%), Positives = 40/65 (61%), Gaps = 1/65 (1%)

Query: 108 KIRIEEMELKLWEKSQQLKSEMKTINPSSQGSAKKLFSTIIGNLKLSISNIHIRYEDGES 167
           K+ I   EL  +EKS+++  +++ ++PS +    ++FST + +L+ S+   ++ +E  E+
Sbjct: 368 KLGITYFELVDYEKSEEVFQKLRDLSPS-RVKDMEVFSTALWHLQKSVPLSYLAHETLET 426

Query: 168 NPGHP 172
           NP  P
Sbjct: 427 NPYSP 431


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.317    0.134    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 186,194,502
Number of Sequences: 164201
Number of extensions: 8042604
Number of successful extensions: 21529
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 21472
Number of HSP's gapped (non-prelim): 57
length of query: 1631
length of database: 59,974,054
effective HSP length: 124
effective length of query: 1507
effective length of database: 39,613,130
effective search space: 59696986910
effective search space used: 59696986910
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 73 (32.7 bits)