BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0546.8
(898 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AFT1_YEAST (P22149) Iron-regulated transcriptional repressor AFT1 38 0.14
H14_CAEEL (O17536) Histone H1.4 (Histone H1-like protein 4) 35 0.68
YG36_YEAST (P53274) Hypothetical 25.8 kDa protein in ASN2-PHB1 i... 35 1.2
HIS8_LACLA (Q02135) Histidinol-phosphate aminotransferase (EC 2.... 33 2.6
DAK1_YEAST (P54838) Dihydroxyacetone kinase 1 (EC 2.7.1.29) (Gly... 33 2.6
TRA0_MYCSM (P35883) Transposase for insertion sequence element I... 33 3.4
NEUM_CHICK (P35001) Neuromodulin (Axonal membrane protein GAP-43... 33 3.4
HMGC_MOUSE (P52927) High mobility group protein HMGI-C (High mob... 33 3.4
H11_VOLCA (Q08864) Histone H1-I 33 3.4
WR51_ARATH (Q93WU9) Probable WRKY transcription factor 51 (WRKY ... 33 4.4
KCC4_YEAST (P25389) Probable serine/threonine-protein kinase KCC... 33 4.4
H1_ONCMY (P06350) Histone H1 [Contains: Oncorhyncin II] 33 4.4
CFAI_HUMAN (P05156) Complement factor I precursor (EC 3.4.21.45)... 32 5.8
HMGC_HUMAN (P52926) High mobility group protein HMGI-C (High mob... 32 7.6
CTCF_RAT (Q9R1D1) Transcriptional repressor CTCF (CCCTC-binding ... 32 7.6
CTCF_MOUSE (Q61164) Transcriptional repressor CTCF (CCCTC-bindin... 32 7.6
CTCF_HUMAN (P49711) Transcriptional repressor CTCF (CCCTC-bindin... 32 7.6
BPA1_MOUSE (Q91ZU6) Bullous pemphigoid antigen 1, isoforms 1/2/3... 32 7.6
VNUC_IAME5 (Q07548) Nucleoprotein 32 9.9
TCPQ_HUMAN (P50990) T-complex protein 1, theta subunit (TCP-1-th... 32 9.9
>AFT1_YEAST (P22149) Iron-regulated transcriptional repressor AFT1
Length = 690
Score = 37.7 bits (86), Expect = 0.14
Identities = 22/80 (27%), Positives = 41/80 (50%), Gaps = 13/80 (16%)
Query: 74 KRNRTGTKKCNCPFRLRARRSNKDKMWTVLVHSGIHNHDTAEVLQGHSYVGRLNPEEKAM 133
K+ R ++ NCPFR+RA S K K W+++V H+H + NP+ +
Sbjct: 204 KKKRCVSRFNNCPFRVRATYSLKRKRWSIVVMDNNHSHQL-----------KFNPDSEEY 252
Query: 134 VGEMIEERVKASDILIAIRK 153
+ +E+++ + + AI+K
Sbjct: 253 --KKFKEKLRKDNDVDAIKK 270
>H14_CAEEL (O17536) Histone H1.4 (Histone H1-like protein 4)
Length = 252
Score = 35.4 bits (80), Expect = 0.68
Identities = 16/29 (55%), Positives = 23/29 (79%)
Query: 649 KASKASKASKASKVSTASKASKVQSPLIR 677
KASKA+KASKA+K S A+KA + P+++
Sbjct: 18 KASKATKASKATKASKATKAKTTKVPMVK 46
Score = 33.1 bits (74), Expect = 3.4
Identities = 19/48 (39%), Positives = 26/48 (53%), Gaps = 7/48 (14%)
Query: 639 QEPSPKVRSQKASKASKASKASKVSTAS-------KASKVQSPLIRMV 679
+ P+ ++ KA+KASKA+KASK + A KA P I MV
Sbjct: 11 ETPAAPTKASKATKASKATKASKATKAKTTKVPMVKADAAHPPFINMV 58
Score = 33.1 bits (74), Expect = 3.4
Identities = 16/29 (55%), Positives = 23/29 (79%)
Query: 651 SKASKASKASKVSTASKASKVQSPLIRMV 679
+KASKA+KASK + ASKA+K ++ + MV
Sbjct: 17 TKASKATKASKATKASKATKAKTTKVPMV 45
>YG36_YEAST (P53274) Hypothetical 25.8 kDa protein in ASN2-PHB1
intergenic region
Length = 230
Score = 34.7 bits (78), Expect = 1.2
Identities = 18/48 (37%), Positives = 26/48 (53%)
Query: 657 SKASKVSTASKASKVQSPLIRMVCTKNKKPVSSYLRWRYLKQIPGEFH 704
S SKV+T S+ S ++ + M KNKK + Y + + K I G FH
Sbjct: 179 STRSKVTTNSQGSSLEPNKLNMAVEKNKKRIEKYQKHKSEKGIKGFFH 226
>HIS8_LACLA (Q02135) Histidinol-phosphate aminotransferase (EC
2.6.1.9) (Imidazole acetol-phosphate transaminase)
Length = 360
Score = 33.5 bits (75), Expect = 2.6
Identities = 29/111 (26%), Positives = 51/111 (45%), Gaps = 12/111 (10%)
Query: 140 ERVKASDILIAIRKHNPTNLTRIKQIYNEKQAYNRLKRGSLTEMQHLMKLLEEHKYAHWS 199
E+ + +DI+ NP T + Q++NE+ L+ T+ + L K L ++ +
Sbjct: 17 EQPELTDIIKLNTNENPYPPTSVAQLFNERYKTKNLRLYPSTDAKSLRKKLADYHHLEVE 76
Query: 200 RV--QDGTDVVRSLFWAHPDSIHLFNEFPHVVILDSTYK-----TNRYRIP 243
+V +G+D V SL + + FN +++ D TY YRIP
Sbjct: 77 QVIIGNGSDEVLSLSF-----LTFFNSQSPLLMPDITYSFYPIYCELYRIP 122
>DAK1_YEAST (P54838) Dihydroxyacetone kinase 1 (EC 2.7.1.29)
(Glycerone kinase 1) (DHA kinase 1)
Length = 584
Score = 33.5 bits (75), Expect = 2.6
Identities = 13/29 (44%), Positives = 18/29 (61%)
Query: 705 PFVVDFINVKGDGHCGYRCVASLKGLAED 733
P + + N GDG CGY VA +KG+ E+
Sbjct: 404 PHITELDNQVGDGDCGYTLVAGVKGITEN 432
>TRA0_MYCSM (P35883) Transposase for insertion sequence element
IS6120
Length = 323
Score = 33.1 bits (74), Expect = 3.4
Identities = 48/217 (22%), Positives = 90/217 (41%), Gaps = 35/217 (16%)
Query: 217 DSIHLFNEFPHVVILDSTYKTNRYRIPLLEMVGVTSTNLTYSIAFAYMQNEQKDEVVWAV 276
D ++L+ + H+ + + ++ ++ LL M+GV + +A E + WA
Sbjct: 60 DYVYLWVDGIHLKV-----RLDQEKLCLLVMLGVRADGRKELVAITDGYRESAES--WA- 111
Query: 277 NRLKDLIINEDNLPKVFVTDKDQVLMEALETVFPAARCLLCQFHVLKSVTGEMKK----- 331
+ L+D P + + D +A+ VFPA + C FH +V + K
Sbjct: 112 DLLRDCKRRGMTAPVLAIGDGALGFWKAVREVFPATKEQRCWFHKQANVLAALPKSAHPS 171
Query: 332 ---LVKDIETREDITDRWNRLMYAANEVE----YDKAVGAMSNTGELWTYIETNWLPLRS 384
+K+I EDI ++ A E + Y KAV + T+ L +
Sbjct: 172 ALAAIKEIYNAEDIDKA--QIAVKAFEADFGAKYPKAVAKI-----------TDDLDVLL 218
Query: 385 KFVKFEIDTCMHMGCTTTNRVEGAHSKLKKLLSDSKG 421
+F K+ + +H+ TTN +E + ++ +KG
Sbjct: 219 EFYKYPAEHWIHL--RTTNPIESTFATVRLRTKVTKG 253
>NEUM_CHICK (P35001) Neuromodulin (Axonal membrane protein GAP-43)
(PP46) (B-50) (Protein F1) (Calmodulin-binding protein
P-57)
Length = 246
Score = 33.1 bits (74), Expect = 3.4
Identities = 36/153 (23%), Positives = 62/153 (39%), Gaps = 7/153 (4%)
Query: 541 GLSFESEFDKLRTKFEESSHG-VRMSIIESLRMITYPETTSMCAPTKKKTRGRPMGSTNK 599
G+ E + K TK + S G + ++ + P + S A KK G G
Sbjct: 25 GIKPEDKAHKAATKIQASFRGHITRKKLKGEKKADAPASESEAAD--KKDEGPAGGRAEN 82
Query: 600 PKKFAKGCGETSTKRIPCRWE-TIDKEYPGSWRDSN---TTPLQEPSPKVRSQKASKASK 655
+ A E S E + D P + N T ++P+P+ + AS K
Sbjct: 83 KESEASTATEASAADSAQLDEGSKDSSVPTEEKKGNGAADTGSEQPAPQAATPAASSEEK 142
Query: 656 ASKASKVSTASKASKVQSPLIRMVCTKNKKPVS 688
+ A++ +A+KAS SP ++ K K+ ++
Sbjct: 143 PAAAAETESATKASTDNSPSLKADEAKTKRSLN 175
>HMGC_MOUSE (P52927) High mobility group protein HMGI-C (High
mobility group AT-hook 2)
Length = 108
Score = 33.1 bits (74), Expect = 3.4
Identities = 17/36 (47%), Positives = 22/36 (60%), Gaps = 1/36 (2%)
Query: 582 CAPTKKKTRGRPMGSTNK-PKKFAKGCGETSTKRIP 616
C P+ K+ RGRP GS NK P K A+ ET ++ P
Sbjct: 41 CEPSPKRPRGRPKGSKNKSPSKAAQKKAETIGEKRP 76
>H11_VOLCA (Q08864) Histone H1-I
Length = 260
Score = 33.1 bits (74), Expect = 3.4
Identities = 28/103 (27%), Positives = 43/103 (41%), Gaps = 2/103 (1%)
Query: 583 APTKKKTRGRPMGSTNKPKKFAKGCGETSTKRIPCRWETIDKEYPGSWRDSNTTPLQEPS 642
A KKT+ P +PKK K E K P + P S TP + +
Sbjct: 160 AEAVKKTKA-PKEKVERPKKEKKEKVEKK-KATPKAEKPKKAATPKSAGKKKATPKPKAA 217
Query: 643 PKVRSQKASKASKASKASKVSTASKASKVQSPLIRMVCTKNKK 685
PK ++K +K KA+ + K + +K +P + +K KK
Sbjct: 218 PKSPAKKDAKPKKATPSKKAAPKKAPAKKSTPKAKEAKSKGKK 260
>WR51_ARATH (Q93WU9) Probable WRKY transcription factor 51 (WRKY
DNA-binding protein 51)
Length = 194
Score = 32.7 bits (73), Expect = 4.4
Identities = 13/50 (26%), Positives = 22/50 (44%)
Query: 64 GKYKPSTSVLKRNRTGTKKCNCPFRLRARRSNKDKMWTVLVHSGIHNHDT 113
GK ++ KRN C + R R D + + + G+HNH++
Sbjct: 119 GKKSVKNNINKRNYYKCSSEGCSVKKRVERDGDDAAYVITTYEGVHNHES 168
>KCC4_YEAST (P25389) Probable serine/threonine-protein kinase KCC4
(EC 2.7.1.37)
Length = 1037
Score = 32.7 bits (73), Expect = 4.4
Identities = 27/132 (20%), Positives = 47/132 (35%), Gaps = 2/132 (1%)
Query: 550 KLRTKFEESSHGVRMSIIESLRMITYPETTSMCAPTKK--KTRGRPMGSTNKPKKFAKGC 607
K R K +++ R S + S + + P ++ K R S+ K F
Sbjct: 374 KNRNKIKKTKKNKRSSTLSSSSSLLLNNRSIQSTPRRRTSKRHSREFSSSRKRSSFLLSS 433
Query: 608 GETSTKRIPCRWETIDKEYPGSWRDSNTTPLQEPSPKVRSQKASKASKASKASKVSTASK 667
T + IP R + ++ TP P+P R+ K + + S +S
Sbjct: 434 NPTDSSPIPLRSSKRITHINVASANTQATPSGVPNPHKRNSKKRSSKRLSYMPNTKRSSL 493
Query: 668 ASKVQSPLIRMV 679
SK S ++
Sbjct: 494 TSKSLSNFTNLI 505
>H1_ONCMY (P06350) Histone H1 [Contains: Oncorhyncin II]
Length = 206
Score = 32.7 bits (73), Expect = 4.4
Identities = 25/92 (27%), Positives = 44/92 (47%), Gaps = 8/92 (8%)
Query: 583 APTKKKTRGRPMGSTNKPKKFAKGCGETSTKRIPCRWETIDKEYPGSWRDSNTTPLQEPS 642
AP KK + + KPKK A + K + + + P + + + +P +
Sbjct: 114 APKAKKVAAKKPAAAKKPKKVA------AKKAVAAKKSPKKAKKPATPKKAAKSPKKVKK 167
Query: 643 PKVRSQKASKA-SKASKASKVSTAS-KASKVQ 672
P ++KA+K+ KA+KA+K A KA+K +
Sbjct: 168 PAAAAKKAAKSPKKATKAAKPKAAKPKAAKAK 199
>CFAI_HUMAN (P05156) Complement factor I precursor (EC 3.4.21.45)
(C3B/C4B inactivator)
Length = 583
Score = 32.3 bits (72), Expect = 5.8
Identities = 18/65 (27%), Positives = 28/65 (42%), Gaps = 11/65 (16%)
Query: 185 HLMKLLEEHKYAHWSRVQDGTDVVRSLFWAHPDSIHLFNEFPHVVILDSTYKTNRYR--I 242
H ++ + H+Y W+ V D W HPD + E+ +I Y Y+ I
Sbjct: 380 HCLRASKTHRYQIWTTVVD---------WIHPDLKRIVIEYVDRIIFHENYNAGTYQNDI 430
Query: 243 PLLEM 247
L+EM
Sbjct: 431 ALIEM 435
>HMGC_HUMAN (P52926) High mobility group protein HMGI-C (High
mobility group AT-hook 2)
Length = 109
Score = 32.0 bits (71), Expect = 7.6
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 10/72 (13%)
Query: 584 PTKKKTRGRPMGSTNK-PKKFAKGCGETSTKRIPCRWETIDKEYPGSWRDS--NTTPLQE 640
P+ K+ RGRP GS NK P K A+ E + ++ P + P W P QE
Sbjct: 43 PSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRP-------RGRPRKWPQQVVQKKPAQE 95
Query: 641 PSPKVRSQKASK 652
+ + SQ++++
Sbjct: 96 ETEETSSQESAE 107
>CTCF_RAT (Q9R1D1) Transcriptional repressor CTCF (CCCTC-binding
factor) (CTCFL paralog) (11-zinc finger protein)
Length = 737
Score = 32.0 bits (71), Expect = 7.6
Identities = 13/27 (48%), Positives = 17/27 (62%)
Query: 576 PETTSMCAPTKKKTRGRPMGSTNKPKK 602
P+ + P KK RGRP G TN+PK+
Sbjct: 648 PQPVAPAPPPAKKRRGRPPGRTNQPKQ 674
>CTCF_MOUSE (Q61164) Transcriptional repressor CTCF (CCCTC-binding
factor) (CTCFL paralog) (11-zinc finger protein)
Length = 736
Score = 32.0 bits (71), Expect = 7.6
Identities = 13/27 (48%), Positives = 17/27 (62%)
Query: 576 PETTSMCAPTKKKTRGRPMGSTNKPKK 602
P+ + P KK RGRP G TN+PK+
Sbjct: 647 PQPVAPAPPPAKKRRGRPPGRTNQPKQ 673
>CTCF_HUMAN (P49711) Transcriptional repressor CTCF (CCCTC-binding
factor) (CTCFL paralog) (11-zinc finger protein)
Length = 727
Score = 32.0 bits (71), Expect = 7.6
Identities = 13/27 (48%), Positives = 17/27 (62%)
Query: 576 PETTSMCAPTKKKTRGRPMGSTNKPKK 602
P+ + P KK RGRP G TN+PK+
Sbjct: 638 PQPVTPAPPPAKKRRGRPPGRTNQPKQ 664
>BPA1_MOUSE (Q91ZU6) Bullous pemphigoid antigen 1, isoforms 1/2/3/4
(BPA) (Hemidesmosomal plaque protein) (Dystonia
musculorum protein) (Dystonin)
Length = 7389
Score = 32.0 bits (71), Expect = 7.6
Identities = 61/305 (20%), Positives = 113/305 (37%), Gaps = 38/305 (12%)
Query: 116 VLQGHSYVGRLNPEEKAMVGEMIEERVKASDILIAIRKHNPTNLTRIKQIYNE-KQAYNR 174
V GH + EEK + + +++ +K D + I L R + + ++ + Y
Sbjct: 5707 VTSGHKIMTTSGEEEKQSMKKKLDKVLKKYDAVCQINSERHLQLERAQSLVSQFWETYEE 5766
Query: 175 LKRGSLTEMQHLMKLLEEH--KYAHWSRVQDGTDVVRSLFWAHPDSIHLFNEFPHVVILD 232
L LTE Q ++ L +Y + R Q+ +R L H PH+ D
Sbjct: 5767 LWPW-LTETQRIISQLPAPALEYETFERQQEEHRQLRELIAEHK---------PHI---D 5813
Query: 233 STYKTNRYRIPLLEMVGVTSTNLTYSIAFAYMQNEQKDEVVWAVNRLKDLIINEDNLPKV 292
KT + L G+ +A + ++ K++V + + ++++E
Sbjct: 5814 KMNKTGPQLLELSPKEGIYIQE--KYVAADTLYSQIKEDV-----KKRAVVLDEAISQST 5866
Query: 293 FVTDKDQVLMEALETVFPAAR---CLLCQFHVLKSVTGEMKKLVKDIE----TREDITDR 345
DK ++E+LE + R + + +K GE K + D+E E + R
Sbjct: 5867 QFHDKIDQILESLERIAERLRQPPSISAEVEKIKEQIGENKSVSVDMEKLQPLYETLRQR 5926
Query: 346 WNRLMYAANEVEYDKAVGAMSNT--------GELWTYIETNWLPLRSKFVKFEIDTCMHM 397
++ + E D + A+ + G + T +E L E C HM
Sbjct: 5927 GEEMIARSEGTEKDVSARAVQDKLDQMVFIWGSIHTLVEEREAKLLDVMELAEKFWCDHM 5986
Query: 398 GCTTT 402
T
Sbjct: 5987 SLVVT 5991
>VNUC_IAME5 (Q07548) Nucleoprotein
Length = 498
Score = 31.6 bits (70), Expect = 9.9
Identities = 34/148 (22%), Positives = 61/148 (40%), Gaps = 12/148 (8%)
Query: 568 ESLRMITYPETTSMCAPTKKKTRGRPMGSTNKPKKFAKGCGETSTKRIPCRWETIDKEYP 627
E LR++++ T +C K TRG + S E+ST + R+ I
Sbjct: 339 EDLRLLSFIRGTKVCPRGKLSTRGVQIASNENMDNM-----ESSTLELRSRYWAIRTRSG 393
Query: 628 GSWRDSNTTPLQ---EPSPKVRSQKASKASKASKASKVSTASKASKVQSPLIRMVCTKNK 684
G+ + Q +P+ V+ + S A +T + S +++ +IRM+ +
Sbjct: 394 GNTNQQRASAGQISVQPTFSVQRNLPFEKSTVMAAFTGNTEGRTSDMRAEIIRMM--EGA 451
Query: 685 KPVSSYLRWRYLKQIPGE--FHPFVVDF 710
KP R R + ++ E +P V F
Sbjct: 452 KPEEVSFRGRGVFELSDEKATNPIVPSF 479
>TCPQ_HUMAN (P50990) T-complex protein 1, theta subunit
(TCP-1-theta) (CCT-theta)
Length = 547
Score = 31.6 bits (70), Expect = 9.9
Identities = 16/61 (26%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
Query: 268 QKDEVVWAVNRLKDLIINEDNLPKVFVTDKDQVLMEALETVFPAARCLLCQFHVLKSVTG 327
Q + N + ++IN +L K+FVT+ ++ LE PAA+ ++ H+ + G
Sbjct: 40 QTTRTAYGPNGMNKMVIN--HLEKLFVTNDAATILRELEVQHPAAKMIVMASHMQEQEVG 97
Query: 328 E 328
+
Sbjct: 98 D 98
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.134 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,464,236
Number of Sequences: 164201
Number of extensions: 4445906
Number of successful extensions: 11474
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 11443
Number of HSP's gapped (non-prelim): 40
length of query: 898
length of database: 59,974,054
effective HSP length: 119
effective length of query: 779
effective length of database: 40,434,135
effective search space: 31498191165
effective search space used: 31498191165
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 70 (31.6 bits)
Lotus: description of TM0546.8