BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0544.11
(200 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MYSP_ANISI (Q9NJA9) Paramyosin (Allergen Ani s 2) 35 0.11
TFT1_MOUSE (O08970) Tuftelin 35 0.15
TFT1_HUMAN (Q9NNX1) Tuftelin 33 0.33
RL29_BUCAK (P46174) 50S ribosomal protein L29 33 0.33
RL29_YERPE (Q8ZJA4) 50S ribosomal protein L29 33 0.44
K1H4_HUMAN (O76011) Keratin, type I cuticular HA4 (Hair keratin,... 33 0.44
TAC1_HUMAN (O75410) Transforming acidic coiled-coil-containing p... 32 0.75
MYSP_SARSC (Q9BMM8) Paramyosin 32 0.75
K1M2_SHEEP (P25690) Keratin, type I microfibrillar, 47.6 kDa (Lo... 32 0.97
EZRI_RABIT (Q8HZQ5) Ezrin (p81) (Cytovillin) (Villin 2) 32 0.97
C5BA_BACTU (Q45712) Pesticidial crystal protein cry5Ba (Insectic... 32 0.97
MYSP_DIRIM (P13392) Paramyosin (Fragment) 32 1.3
MYSP_BRUMA (Q01202) Paramyosin 32 1.3
K1M1_SHEEP (P02534) Keratin, type I microfibrillar 48 kDa, compo... 32 1.3
K1H1_HUMAN (Q15323) Keratin, type I cuticular HA1 (Hair keratin,... 32 1.3
E78A_DROME (P45447) Ecdysone-induced protein 78C (DR-78) 32 1.3
MYSP_ONCVO (Q02171) Paramyosin 31 1.7
ATPF_BACSU (P37814) ATP synthase B chain (EC 3.6.3.14) 31 1.7
Y328_MYCPN (P75310) Hypothetical protein MG328 homolog (P01_orf1... 31 2.2
RL29_VIBCH (Q9KNZ2) 50S ribosomal protein L29 31 2.2
>MYSP_ANISI (Q9NJA9) Paramyosin (Allergen Ani s 2)
Length = 869
Score = 35.0 bits (79), Expect = 0.12
Identities = 29/99 (29%), Positives = 45/99 (45%), Gaps = 3/99 (3%)
Query: 103 NEEMNKDLLTMLSEELNLQRIV--TNETLEQTDRLVMNARNASSHYQMETEKCNVAMETC 160
N E K + + + LQ + T L+QT A+ S E E+C A++
Sbjct: 563 NLEAQKTIKKQSEQIIQLQANLEDTQRQLQQTLDQYALAQRKISALSAELEECKTALDNA 622
Query: 161 EEARERAEAELIEEH-RVTALWENRAREYGWKDKRRTRL 198
AR++AEA+L E H R++ L + K+K T L
Sbjct: 623 IRARKQAEADLEEAHVRISDLTSINSNLTAIKNKLETEL 661
>TFT1_MOUSE (O08970) Tuftelin
Length = 390
Score = 34.7 bits (78), Expect = 0.15
Identities = 29/89 (32%), Positives = 44/89 (48%), Gaps = 11/89 (12%)
Query: 88 SPPDTFDCGKHDPVMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQ 147
SPP+ DP M+E++ L +EL L ++ E Q+DR+ S YQ
Sbjct: 157 SPPEV------DPSMSEDVES--LKKTVQEL-LVKLREAERRHQSDRVAFEV--TLSRYQ 205
Query: 148 METEKCNVAMETCEEARERAEAELIEEHR 176
E E+ NVA++ E+ E+ AE+ E R
Sbjct: 206 REAEQSNVALQREEDRVEQKAAEIEELQR 234
>TFT1_HUMAN (Q9NNX1) Tuftelin
Length = 390
Score = 33.5 bits (75), Expect = 0.33
Identities = 27/89 (30%), Positives = 39/89 (43%), Gaps = 11/89 (12%)
Query: 88 SPPDTFDCGKHDPVMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQ 147
SPP+ C D + +DLL L E + Q+D + S YQ
Sbjct: 157 SPPEVDTCINEDVESLRKTVQDLLAKLQEA---------KRQHQSDCVAFEV--TLSRYQ 205
Query: 148 METEKCNVAMETCEEARERAEAELIEEHR 176
E E+ NVA++ E+ E+ EAE+ E R
Sbjct: 206 REAEQSNVALQREEDRVEQKEAEVGELQR 234
>RL29_BUCAK (P46174) 50S ribosomal protein L29
Length = 63
Score = 33.5 bits (75), Expect = 0.33
Identities = 19/52 (36%), Positives = 28/52 (53%)
Query: 104 EEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNV 155
EE+N +LL +L E+ NL+ + L+QT L RN + + TEK V
Sbjct: 12 EELNTELLNLLREQFNLRMQGASGQLQQTHLLKQVRRNVARVKTLLTEKAGV 63
>RL29_YERPE (Q8ZJA4) 50S ribosomal protein L29
Length = 63
Score = 33.1 bits (74), Expect = 0.44
Identities = 18/49 (36%), Positives = 27/49 (54%)
Query: 104 EEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEK 152
EE+N +LL +L E+ NL+ + L+QT L RN + + TEK
Sbjct: 12 EELNTELLNLLREQFNLRMQAASGQLQQTHLLKQVRRNVARVKTLLTEK 60
>K1H4_HUMAN (O76011) Keratin, type I cuticular HA4 (Hair keratin,
type I HA4)
Length = 394
Score = 33.1 bits (74), Expect = 0.44
Identities = 27/99 (27%), Positives = 47/99 (47%), Gaps = 5/99 (5%)
Query: 102 MNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLV-MNARNASSHYQMETEKCNVAMETC 160
+ + +N ++ T + +LN V NET Q + LV +N R + +TE+ N + +
Sbjct: 205 LGDRLNVEVDTAPTVDLNQ---VLNETRSQYEALVEINRREVEQWFATQTEELNKQVVSS 261
Query: 161 EEARERAEAELIEEHR-VTALWENRAREYGWKDKRRTRL 198
E + +AE+IE R V AL ++ +D L
Sbjct: 262 SEQLQSCQAEIIELRRTVNALEIELQAQHNLRDSLENTL 300
>TAC1_HUMAN (O75410) Transforming acidic coiled-coil-containing
protein 1
Length = 805
Score = 32.3 bits (72), Expect = 0.75
Identities = 21/71 (29%), Positives = 35/71 (48%), Gaps = 7/71 (9%)
Query: 84 SSAESPPDTFDCGKHDPV----MNEEMNKDLLTMLSEELNLQRIVTNE---TLEQTDRLV 136
SSAE P + CG P+ ++E +LT++ EE+ + I NE E+T + V
Sbjct: 576 SSAEKAPVSVSCGGESPLDGICLSESDKTAVLTLIREEIITKEIEANEWKKKYEETRQEV 635
Query: 137 MNARNASSHYQ 147
+ R + Y+
Sbjct: 636 LEMRKIVAEYE 646
>MYSP_SARSC (Q9BMM8) Paramyosin
Length = 876
Score = 32.3 bits (72), Expect = 0.75
Identities = 21/84 (25%), Positives = 38/84 (45%)
Query: 101 VMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAMETC 160
V +M D L ++L Q T LE+ +R + N + ++E E V ++
Sbjct: 215 VHEHKMQLDNANHLKQQLAQQLEDTKHRLEEEERKRASLENHAHTLEVELESLKVQLDEE 274
Query: 161 EEARERAEAELIEEHRVTALWENR 184
EAR E +L + + A W+++
Sbjct: 275 SEARLELERQLTKANGDAASWKSK 298
>K1M2_SHEEP (P25690) Keratin, type I microfibrillar, 47.6 kDa
(Low-sulfur keratin)
Length = 403
Score = 32.0 bits (71), Expect = 0.97
Identities = 29/107 (27%), Positives = 47/107 (43%), Gaps = 11/107 (10%)
Query: 103 NEEMNKDLLTMLSEELNLQ---------RIVTNETLEQTDRLV-MNARNASSHYQMETEK 152
+E+ L + L + LN++ V NET Q + LV N R+ Y +TE+
Sbjct: 193 HEQEVNTLRSQLGDRLNVEVDAAPTVDLNHVLNETRAQYEALVETNRRDVEEWYIRQTEE 252
Query: 153 CNVAMETCEEARERAEAELIEEHR-VTALWENRAREYGWKDKRRTRL 198
N + + E + +AE+IE R V AL ++ +D L
Sbjct: 253 LNKQVVSSSEQLQSCQAEIIELRRTVNALEVELQAQHNLRDSLENTL 299
>EZRI_RABIT (Q8HZQ5) Ezrin (p81) (Cytovillin) (Villin 2)
Length = 585
Score = 32.0 bits (71), Expect = 0.97
Identities = 20/73 (27%), Positives = 31/73 (42%), Gaps = 7/73 (9%)
Query: 115 SEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAEAELIEE 174
SE L R+ E+ +R + + E + + EEAR R E+E+ E
Sbjct: 385 SERLEADRVAALRAKEELERQAADQIKSQEQLAAELAEYTAKIALLEEARRRKESEVEE- 443
Query: 175 HRVTALWENRARE 187
W++RARE
Sbjct: 444 ------WQHRARE 450
>C5BA_BACTU (Q45712) Pesticidial crystal protein cry5Ba
(Insecticidal delta-endotoxin CryVB(a)) (Crystaline
entomocidal protoxin) (140 kDa crystal protein)
Length = 1245
Score = 32.0 bits (71), Expect = 0.97
Identities = 27/119 (22%), Positives = 47/119 (38%), Gaps = 5/119 (4%)
Query: 40 VHITCESPSDIVQVIVEFGPLSRWRLKESASTESPCHHPCDCYCSSAESPPDTFDCGKHD 99
VHIT + SD+ +EF P +++ ++ + C++P D C+ DC ++
Sbjct: 676 VHITNQGSSDLFLDRIEFVPKIQFQFCDNNNLHCDCNNPVDTDCTFCCVCTSLTDCDCNN 735
Query: 100 PVMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAME 158
P D E L VT L+ V +S H + T+ + +E
Sbjct: 736 P-----RGLDCTLCCQVENQLPSFVTLTDLQNITTQVNALVASSEHDTLATDVSDYEIE 789
>MYSP_DIRIM (P13392) Paramyosin (Fragment)
Length = 848
Score = 31.6 bits (70), Expect = 1.3
Identities = 27/88 (30%), Positives = 42/88 (47%), Gaps = 4/88 (4%)
Query: 115 SEELNLQRIVTNET---LEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAEAEL 171
SE+L + + +T L+QT A+ S E E+C VA++ AR++AE +L
Sbjct: 553 SEQLKILQASLEDTQRQLQQTLDQYALAQRKVSALSAELEECKVALDNAIRARKQAEIDL 612
Query: 172 IEEH-RVTALWENRAREYGWKDKRRTRL 198
E + R+T L K+K T L
Sbjct: 613 EEANARITDLVSINNNLTAIKNKLETEL 640
>MYSP_BRUMA (Q01202) Paramyosin
Length = 880
Score = 31.6 bits (70), Expect = 1.3
Identities = 27/88 (30%), Positives = 42/88 (47%), Gaps = 4/88 (4%)
Query: 115 SEELNLQRIVTNET---LEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAEAEL 171
SE+L + + +T L+QT A+ S E E+C VA++ AR++AE +L
Sbjct: 580 SEQLKILQASLEDTQRQLQQTLDQYALAQRKVSALSAELEECKVALDNAIRARKQAEIDL 639
Query: 172 IEEH-RVTALWENRAREYGWKDKRRTRL 198
E + R+T L K+K T L
Sbjct: 640 EEANGRITDLVSVNNNLTAIKNKLETEL 667
>K1M1_SHEEP (P02534) Keratin, type I microfibrillar 48 kDa,
component 8C-1 (Low-sulfur keratin)
Length = 412
Score = 31.6 bits (70), Expect = 1.3
Identities = 29/107 (27%), Positives = 46/107 (42%), Gaps = 11/107 (10%)
Query: 103 NEEMNKDLLTMLSEELNLQ---------RIVTNETLEQTDRLV-MNARNASSHYQMETEK 152
+EE L + L + LN++ V NET Q + LV N R+ Y +TE+
Sbjct: 193 HEEEVNTLRSQLGDRLNVEVDAAPTVDLNRVLNETRAQYEALVETNRRDVEEWYIRQTEE 252
Query: 153 CNVAMETCEEARERAEAELIEEHR-VTALWENRAREYGWKDKRRTRL 198
N + + E + + E+IE R V AL ++ +D L
Sbjct: 253 LNKQVVSSSEQLQSCQTEIIELRRTVNALEVELQAQHNLRDSLENTL 299
>K1H1_HUMAN (Q15323) Keratin, type I cuticular HA1 (Hair keratin,
type I HA1)
Length = 416
Score = 31.6 bits (70), Expect = 1.3
Identities = 31/120 (25%), Positives = 52/120 (42%), Gaps = 10/120 (8%)
Query: 81 CYCSSAESPPDTFDCGKHDPVMNEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLV-MNA 139
C S+ E +T C + + +N ++ + +LN V NET Q + LV N
Sbjct: 189 CLKSNHEQEVNTLRCQ-----LGDRLNVEVDAAPTVDLNR---VLNETRSQYEALVETNR 240
Query: 140 RNASSHYQMETEKCNVAMETCEEARERAEAELIEEHR-VTALWENRAREYGWKDKRRTRL 198
R + +TE+ N + + E + +AE+IE R V AL ++ +D L
Sbjct: 241 REVEQWFTTQTEELNKQVVSSSEQLQSYQAEIIELRRTVNALEIELQAQHNLRDSLENTL 300
>E78A_DROME (P45447) Ecdysone-induced protein 78C (DR-78)
Length = 866
Score = 31.6 bits (70), Expect = 1.3
Identities = 15/46 (32%), Positives = 21/46 (45%)
Query: 51 VQVIVEFGPLSRWRLKESASTESPCHHPCDCYCSSAESPPDTFDCG 96
+ + +FGP S S+ + S H D CSSA S + CG
Sbjct: 225 ISSVHQFGPASNHNTSSSSPSSSAAHSSPDSGCSSASSSGSSRSCG 270
>MYSP_ONCVO (Q02171) Paramyosin
Length = 879
Score = 31.2 bits (69), Expect = 1.7
Identities = 24/75 (32%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Query: 125 TNETLEQTDRLVMNARNASSHYQMETEKCNVAMETCEEARERAEAELIEEH-RVTALWEN 183
T L+QT A+ S E E+C VA++ AR++AE +L E + R+T L
Sbjct: 593 TQRQLQQTLDQYALAQRKVSALSAELEECKVALDNAIRARKQAEIDLEEANGRITDLVSI 652
Query: 184 RAREYGWKDKRRTRL 198
K+K T L
Sbjct: 653 NNNLTAIKNKLETEL 667
>ATPF_BACSU (P37814) ATP synthase B chain (EC 3.6.3.14)
Length = 170
Score = 31.2 bits (69), Expect = 1.7
Identities = 23/84 (27%), Positives = 40/84 (47%), Gaps = 8/84 (9%)
Query: 104 EEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAMETCEEA 163
EE NK+ ++ E QR++ E +++ L+ NA+ + + E A E
Sbjct: 58 EEKNKEAQQLIEE----QRVLLKEARQESQTLIENAKKLGE--KQKEEIIQAARAESERL 111
Query: 164 RERAEAELI--EEHRVTALWENRA 185
+E A E++ +E V+AL E A
Sbjct: 112 KEAARTEIVKEKEQAVSALREQVA 135
>Y328_MYCPN (P75310) Hypothetical protein MG328 homolog
(P01_orf1033)
Length = 1033
Score = 30.8 bits (68), Expect = 2.2
Identities = 25/89 (28%), Positives = 43/89 (48%), Gaps = 6/89 (6%)
Query: 103 NEEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEKCNVAM----- 157
NEE+ + +E +L R+ TN T+ ++ + A QM+ E N+A
Sbjct: 769 NEELTDKCSNIQNELHDLNRVKTNWENLNTEHNLLQDKYAQQKEQMQHEHSNLAQIQAEH 828
Query: 158 ETCEEARERAEAELIEEHRVTALWENRAR 186
E +E+ + +AEL E ++T L E A+
Sbjct: 829 ELLQESYNKVKAEL-NEIQITNLNEANAQ 856
>RL29_VIBCH (Q9KNZ2) 50S ribosomal protein L29
Length = 63
Score = 30.8 bits (68), Expect = 2.2
Identities = 17/49 (34%), Positives = 26/49 (52%)
Query: 104 EEMNKDLLTMLSEELNLQRIVTNETLEQTDRLVMNARNASSHYQMETEK 152
EE+N +LL +L E+ NL+ L+QT L R+ + + TEK
Sbjct: 12 EELNSELLNLLKEQFNLRMQAATGQLQQTHTLKAVRRDIARVKTVLTEK 60
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.128 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,919,718
Number of Sequences: 164201
Number of extensions: 801782
Number of successful extensions: 2762
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 28
Number of HSP's that attempted gapping in prelim test: 2735
Number of HSP's gapped (non-prelim): 57
length of query: 200
length of database: 59,974,054
effective HSP length: 105
effective length of query: 95
effective length of database: 42,732,949
effective search space: 4059630155
effective search space used: 4059630155
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0544.11