BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0544.1
(309 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SECY_CYACA (P46249) Preprotein translocase secY subunit 33 0.90
FTH2_HAEIN (P45219) Cell division protein ftsH homolog 2 (EC 3.4... 33 0.90
FTH1_HAEIN (P71377) Cell division protein ftsH homolog 1 (EC 3.4... 33 0.90
VGL2_CVBV (P25194) E2 glycoprotein precursor (Spike glycoprotein... 31 3.4
VGL2_CVBM (P15777) E2 glycoprotein precursor (Spike glycoprotein... 31 3.4
VGL2_CVBLY (P25192) E2 glycoprotein precursor (Spike glycoprotei... 31 3.4
VGL2_CVBL9 (P25191) E2 glycoprotein precursor (Spike glycoprotei... 31 3.4
VGL2_CVBF (P25190) E2 glycoprotein precursor (Spike glycoprotein... 31 3.4
VGL2_CVBQ (P25193) E2 glycoprotein precursor (Spike glycoprotein... 31 4.5
RK14_ARATH (P56792) Chloroplast 50S ribosomal protein L14 31 4.5
GRPE_BACSH (O69267) GrpE protein (HSP-70 cofactor) 31 4.5
SWC1_YEAST (P31376) SWC1 protein 30 5.8
GRPE_XYLFA (Q9PB04) GrpE protein (HSP-70 cofactor) 30 5.8
CYPH_CANAL (P22011) Peptidyl-prolyl cis-trans isomerase (EC 5.2.... 30 7.6
VGL2_CVHOC (P36334) E2 glycoprotein precursor (Spike glycoprotei... 30 9.9
SYFA_METJA (Q57911) Phenylalanyl-tRNA synthetase alpha chain (EC... 30 9.9
RAGE_BOVIN (Q28173) Advanced glycosylation end product-specific ... 30 9.9
LE11_ARCFU (O29305) 2-isopropylmalate synthase 1 (EC 2.3.3.13) (... 30 9.9
FTSH_CAPAN (Q39444) Cell division protein ftsH homolog, chloropl... 30 9.9
>SECY_CYACA (P46249) Preprotein translocase secY subunit
Length = 410
Score = 33.1 bits (74), Expect = 0.90
Identities = 18/59 (30%), Positives = 28/59 (46%), Gaps = 3/59 (5%)
Query: 126 WNAFGISPQQLFYISLGLLFLWTLDTVSY---GGGLGNLVVDTIGHSFSKKYHNRVIQH 181
WN + I L IS +L +W D +SY G G ++ I +F+K N++ H
Sbjct: 133 WNLYFIVQTTLILISGAMLVMWLADNISYKGIGTGASVIIFVNIASAFAKFLLNQLFVH 191
>FTH2_HAEIN (P45219) Cell division protein ftsH homolog 2 (EC
3.4.24.-)
Length = 381
Score = 33.1 bits (74), Expect = 0.90
Identities = 31/110 (28%), Positives = 48/110 (43%), Gaps = 18/110 (16%)
Query: 181 HEAGHFLIAYLVGILPKGYTLSSLDALKKDGSLNVQAGSAFVDFEFLEEVNAGKVSAKTL 240
HEAGH ++ YLV P+ + + + + +L V FL E + +S K L
Sbjct: 159 HEAGHAIVGYLV---PEHDPVHKVTIIPRGRALGVTF--------FLPEGDQISISQKQL 207
Query: 241 NKFSCIALAGVCTEYLIYG---FSEGGLDDIRKLDSLLSGL----GFTQK 283
AG E LIYG S G +DI+ ++ + GF++K
Sbjct: 208 ESKLSTLYAGRLAEDLIYGEENISTGASNDIKVATNIARNMVTQWGFSEK 257
>FTH1_HAEIN (P71377) Cell division protein ftsH homolog 1 (EC
3.4.24.-)
Length = 635
Score = 33.1 bits (74), Expect = 0.90
Identities = 31/110 (28%), Positives = 48/110 (43%), Gaps = 18/110 (16%)
Query: 181 HEAGHFLIAYLVGILPKGYTLSSLDALKKDGSLNVQAGSAFVDFEFLEEVNAGKVSAKTL 240
HEAGH ++ YLV P+ + + + + +L V FL E + +S K L
Sbjct: 413 HEAGHAIVGYLV---PEHDPVHKVTIIPRGRALGVTF--------FLPEGDQISISQKQL 461
Query: 241 NKFSCIALAGVCTEYLIYG---FSEGGLDDIRKLDSLLSGL----GFTQK 283
AG E LIYG S G +DI+ ++ + GF++K
Sbjct: 462 ESKLSTLYAGRLAEDLIYGEENISTGASNDIKVATNIARNMVTQWGFSEK 511
>VGL2_CVBV (P25194) E2 glycoprotein precursor (Spike glycoprotein)
(Peplomer protein) [Contains: Spike protein S1 (90B);
Spike protein S2 (90A)]
Length = 1363
Score = 31.2 bits (69), Expect = 3.4
Identities = 17/56 (30%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
Query: 210 DGSLNVQAGSAFVDFEFLEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYGFSEGGL 265
D L + F +F L +VN+G + L K + + GVC Y +YG + G+
Sbjct: 590 DSCLQGDRCNIFANF-ILHDVNSGTTCSTDLQKSNTDIILGVCVNYDLYGITGQGI 644
>VGL2_CVBM (P15777) E2 glycoprotein precursor (Spike glycoprotein)
(Peplomer protein) [Contains: Spike protein S1 (90B);
Spike protein S2 (90A)]
Length = 1363
Score = 31.2 bits (69), Expect = 3.4
Identities = 17/56 (30%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
Query: 210 DGSLNVQAGSAFVDFEFLEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYGFSEGGL 265
D L + F +F L +VN+G + L K + + GVC Y +YG + G+
Sbjct: 590 DSCLQGDRCNIFANF-ILHDVNSGTTCSTDLQKSNTDIILGVCVNYDLYGITGQGI 644
>VGL2_CVBLY (P25192) E2 glycoprotein precursor (Spike glycoprotein)
(Peplomer protein) [Contains: Spike protein S1 (90B);
Spike protein S2 (90A)]
Length = 1363
Score = 31.2 bits (69), Expect = 3.4
Identities = 17/56 (30%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
Query: 210 DGSLNVQAGSAFVDFEFLEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYGFSEGGL 265
D L + F +F L +VN+G + L K + + GVC Y +YG + G+
Sbjct: 590 DSCLQGDRCNIFANF-ILHDVNSGTTCSTDLQKSNTDIILGVCVNYDLYGITGQGI 644
>VGL2_CVBL9 (P25191) E2 glycoprotein precursor (Spike glycoprotein)
(Peplomer protein) [Contains: Spike protein S1 (90B);
Spike protein S2 (90A)]
Length = 1363
Score = 31.2 bits (69), Expect = 3.4
Identities = 17/56 (30%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
Query: 210 DGSLNVQAGSAFVDFEFLEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYGFSEGGL 265
D L + F +F L +VN+G + L K + + GVC Y +YG + G+
Sbjct: 590 DSCLQGDRCNIFANF-ILHDVNSGTTCSTDLQKSNTDIILGVCVNYDLYGITGQGI 644
>VGL2_CVBF (P25190) E2 glycoprotein precursor (Spike glycoprotein)
(Peplomer protein) [Contains: Spike protein S1 (90B);
Spike protein S2 (90A)]
Length = 1363
Score = 31.2 bits (69), Expect = 3.4
Identities = 17/56 (30%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
Query: 210 DGSLNVQAGSAFVDFEFLEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYGFSEGGL 265
D L + F +F L +VN+G + L K + + GVC Y +YG + G+
Sbjct: 590 DSCLQGDRCNIFANF-ILHDVNSGTTCSTDLQKSNTDIILGVCVNYDLYGITGQGI 644
>VGL2_CVBQ (P25193) E2 glycoprotein precursor (Spike glycoprotein)
(Peplomer protein) [Contains: Spike protein S1 (90B);
Spike protein S2 (90A)]
Length = 1363
Score = 30.8 bits (68), Expect = 4.5
Identities = 17/56 (30%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
Query: 210 DGSLNVQAGSAFVDFEFLEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYGFSEGGL 265
D L + F +F F +VN+G + L K + + GVC Y +YG + G+
Sbjct: 590 DSCLQGDRCNIFANFIF-HDVNSGTTCSTDLQKSNTDIILGVCVNYDLYGITGQGI 644
>RK14_ARATH (P56792) Chloroplast 50S ribosomal protein L14
Length = 122
Score = 30.8 bits (68), Expect = 4.5
Identities = 19/59 (32%), Positives = 33/59 (55%), Gaps = 5/59 (8%)
Query: 41 LEKLDKELLKGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNGIEAESL 99
L++ + +++ DD AA+ + D +G P G R FGA +P+ L L+ K+ + E L
Sbjct: 69 LKRNNGTIIRYDDNAAVVI--DQEGNPKGTRVFGA---IPRELRQLNFTKIVSLAPEVL 122
>GRPE_BACSH (O69267) GrpE protein (HSP-70 cofactor)
Length = 198
Score = 30.8 bits (68), Expect = 4.5
Identities = 52/202 (25%), Positives = 79/202 (38%), Gaps = 25/202 (12%)
Query: 6 LMHCVGVTSCPGQLKLVRVRIQCSTTNVGVSRRQLLEKLDKELLKGDDRAALALVKDLQG 65
+M + VT LV+ +Q T V R ++ E+++ + + A V +LQ
Sbjct: 2 IMRRIQVTETTENKDLVQGDVQAETATEEVERSEVQEEIELSV----EEQYEANVAELQA 57
Query: 66 KPDG------LRCFGAARQVPQRLYTLDELKLNGIEAESLLSPVDSTLGSIERTLQIAAI 119
K D LR A +R LD A+SLLS + L + ER LQ+
Sbjct: 58 KLDDDEENRHLR-LRADFDNMRRRQQLDGEAAEKYRAQSLLSDLLPVLDNFERALQVETT 116
Query: 120 AGGLTAWNAFGISPQQLFYISLGLLFLWTLDTVSYGGGLGNLVVDTIGHSFSKKYHNRVI 179
+ TA GI ++ Y SL L+ + G V+ G F H V+
Sbjct: 117 SEE-TASIIKGI---EMVYRSL-------LEATVFE---GLQVIKAEGEQFDPNIHQAVM 162
Query: 180 QHEAGHFLIAYLVGILPKGYTL 201
Q + ++ L KGY L
Sbjct: 163 QEQDSEKETGVVLRELQKGYIL 184
>SWC1_YEAST (P31376) SWC1 protein
Length = 625
Score = 30.4 bits (67), Expect = 5.8
Identities = 25/90 (27%), Positives = 40/90 (43%), Gaps = 12/90 (13%)
Query: 43 KLDKELLKGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNGIEAE----- 97
K D++L K D + + K++ P F A P LYT +KL+GI
Sbjct: 509 KADQKLNKEDAKPGSDVEKEVSFNP----LFEA--DCPTPLYTPMTMKLSGIHKRFNQII 562
Query: 98 -SLLSPVDSTLGSIERTLQIAAIAGGLTAW 126
+ +SP++ + +E+ LQI G W
Sbjct: 563 RNSVSPMEEVVKEMEKILQIGTRLSGYNLW 592
>GRPE_XYLFA (Q9PB04) GrpE protein (HSP-70 cofactor)
Length = 172
Score = 30.4 bits (67), Expect = 5.8
Identities = 12/44 (27%), Positives = 24/44 (54%)
Query: 159 GNLVVDTIGHSFSKKYHNRVIQHEAGHFLIAYLVGILPKGYTLS 202
G +++D +G F+ ++H + Q E Y++ + KGY L+
Sbjct: 114 GLMLLDPVGQLFNPEHHQAISQTEVTDVEPGYVIQVFQKGYLLN 157
>CYPH_CANAL (P22011) Peptidyl-prolyl cis-trans isomerase (EC
5.2.1.8) (PPIase) (Rotamase) (Cyclophilin) (Cyclosporin
A-binding protein) (CPH)
Length = 162
Score = 30.0 bits (66), Expect = 7.6
Identities = 12/32 (37%), Positives = 22/32 (68%)
Query: 254 EYLIYGFSEGGLDDIRKLDSLLSGLGFTQKKV 285
+++++G GLD ++K++S SG G T KK+
Sbjct: 123 KHVVFGEVTDGLDIVKKIESFGSGSGATSKKI 154
>VGL2_CVHOC (P36334) E2 glycoprotein precursor (Spike glycoprotein)
(Peplomer protein) [Contains: Spike protein S1 (90B);
Spike protein S2 (90A)]
Length = 1353
Score = 29.6 bits (65), Expect = 9.9
Identities = 17/56 (30%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
Query: 210 DGSLNVQAGSAFVDFEFLEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYGFSEGGL 265
D L + F +F L +VN+G + L K + + GVC Y +YG G+
Sbjct: 580 DSCLQGDKCNIFANF-ILHDVNSGLTCSTDLQKANTDIILGVCVNYDLYGILGQGI 634
>SYFA_METJA (Q57911) Phenylalanyl-tRNA synthetase alpha chain (EC
6.1.1.20) (Phenylalanine--tRNA ligase alpha chain)
(PheRS)
Length = 480
Score = 29.6 bits (65), Expect = 9.9
Identities = 23/77 (29%), Positives = 37/77 (47%), Gaps = 4/77 (5%)
Query: 172 KKYHNRVIQHEAGHFLIAYLVGILPKGYTLSSLDALKKDGSLNVQAGSAF---VDFEFLE 228
+K N + QH I L ILPK ++L A+K+ G ++ G +D++ +E
Sbjct: 70 RKIANYLKQHNIKEIEIKNLKDILPKEEINAALGAIKRKGIARIEKGKIIFDNLDYKDVE 129
Query: 229 EVNAGKVSA-KTLNKFS 244
E K+ K L+ FS
Sbjct: 130 EQLLQKIKENKYLDDFS 146
>RAGE_BOVIN (Q28173) Advanced glycosylation end product-specific
receptor precursor (Receptor for advanced glycosylation
end products)
Length = 416
Score = 29.6 bits (65), Expect = 9.9
Identities = 22/52 (42%), Positives = 27/52 (51%), Gaps = 6/52 (11%)
Query: 65 GKPDGLRCFGAARQVPQRLYTLDELKLN--GIEAESLLSPVDSTLGSIERTL 114
GKP L C GA ++ PQ+L E KLN EA +LSP S+ R L
Sbjct: 31 GKPLVLNCKGAPKKPPQQL----EWKLNTGRTEAWKVLSPQGDPWDSVARVL 78
>LE11_ARCFU (O29305) 2-isopropylmalate synthase 1 (EC 2.3.3.13)
(Alpha-isopropylmalate synthase 1) (Alpha-IPM synthetase
1)
Length = 489
Score = 29.6 bits (65), Expect = 9.9
Identities = 31/135 (22%), Positives = 62/135 (44%), Gaps = 25/135 (18%)
Query: 5 ALMHCVGVTSCPGQLKLVRVRIQCSTTNVGVSRRQLLEKLDKELLKGDDRAALALVKDLQ 64
A+M+ +G + P Q+K + RI+ +G +++ + + +++ L + ++ +
Sbjct: 326 AIMNELGYKATPEQMKEILARIK----EIGDKGKRVTDADVRTIIE----TVLQIKREKK 377
Query: 65 GKPDGLRCFGAARQVPQRLYTLDELKLNG---IEAESLLSPVDSTLGSIERTLQ------ 115
K + L F +P +LK++G IEA L PVD+ + +I R ++
Sbjct: 378 VKLEDLAIFSGKNVMPMASV---KLKIDGQERIEAAVGLGPVDAAINAIRRAIKEFADIK 434
Query: 116 -----IAAIAGGLTA 125
+ AI GG A
Sbjct: 435 LVSYHVDAITGGTDA 449
>FTSH_CAPAN (Q39444) Cell division protein ftsH homolog, chloroplast
precursor (EC 3.4.24.-) (Fragment)
Length = 662
Score = 29.6 bits (65), Expect = 9.9
Identities = 27/113 (23%), Positives = 49/113 (42%), Gaps = 15/113 (13%)
Query: 178 VIQHEAGHFLIAYLVGILPKGYTLSSLDALKKDGSLNVQAGSAFVDFEFLEEVNAGKVSA 237
V HEAGH L+ ++P+ ++ + + + QAG E + +G S
Sbjct: 489 VAYHEAGHALVG---ALMPEYDPVAKISIIPRG-----QAGGLTFFAPSEERLESGLYSR 540
Query: 238 KTLNKFSCIALAGVCTEYLIYG---FSEGGLDDIRKLD----SLLSGLGFTQK 283
L +AL G E +I+G + G +D ++ ++ LGF++K
Sbjct: 541 SYLENQMAVALGGRVAEEVIFGEDNVTTGASNDFMQVSRVARQMVERLGFSKK 593
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.139 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,008,221
Number of Sequences: 164201
Number of extensions: 1459528
Number of successful extensions: 3697
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 3689
Number of HSP's gapped (non-prelim): 19
length of query: 309
length of database: 59,974,054
effective HSP length: 110
effective length of query: 199
effective length of database: 41,911,944
effective search space: 8340476856
effective search space used: 8340476856
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0544.1