BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0520.11
(117 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
GSPI_ECOLI (P45760) Probable general secretion pathway protein I... 30 0.98
LON_HELPY (P55995) ATP-dependent protease La (EC 3.4.21.53) 30 1.3
CIQ3_RAT (O88944) Potassium voltage-gated channel subfamily KQT ... 30 1.3
CIQ3_HUMAN (O43525) Potassium voltage-gated channel subfamily KQ... 30 1.3
CIQ3_BOVIN (P58126) Potassium voltage-gated channel subfamily KQ... 30 1.3
MAP3_SCHPO (P31397) Pheromone M-factor receptor 29 1.7
SAL2_HUMAN (Q9Y467) Sal-like protein 2 (Zinc finger protein SALL... 29 2.2
DXR_PROMA (Q7VB62) 1-deoxy-D-xylulose 5-phosphate reductoisomera... 29 2.2
DF1A_ARATH (Q9SEZ3) Dof zinc finger protein DOF1.10 (AtDOF1.10) ... 29 2.2
DEAD_KLEPN (P33906) Cold-shock DEAD-box protein A (ATP-dependent... 29 2.2
DXR_SYNPX (Q7U8C3) 1-deoxy-D-xylulose 5-phosphate reductoisomera... 28 2.8
DXR_PROMM (Q7V6J8) 1-deoxy-D-xylulose 5-phosphate reductoisomera... 28 2.8
CIQ1_XENLA (P70057) Potassium voltage-gated channel subfamily KQ... 28 3.7
CIQ1_SQUAC (O73925) Potassium voltage-gated channel subfamily KQ... 28 3.7
CIQ1_RAT (Q9Z0N7) Potassium voltage-gated channel subfamily KQT ... 28 3.7
CIQ1_MOUSE (P97414) Potassium voltage-gated channel subfamily KQ... 28 3.7
CIQ1_HUMAN (P51787) Potassium voltage-gated channel subfamily KQ... 28 3.7
PEX6_PENCH (Q9HG03) Peroxisomal biogenesis factor 6 (Peroxin-6) 28 4.9
LON_HELPJ (Q9ZJL3) ATP-dependent protease La (EC 3.4.21.53) 28 4.9
LMA5_MOUSE (Q61001) Laminin alpha-5 chain precursor 28 4.9
>GSPI_ECOLI (P45760) Probable general secretion pathway protein I
precursor
Length = 125
Score = 30.0 bits (66), Expect = 0.98
Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Query: 12 LQRTDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSS--SSTSQCDLPKGQGGNVAKIK 69
LQ DSFGE T KE+ + NW ++ +S + T LP GQ ++ + +
Sbjct: 54 LQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLPSGQTTSLTRYQ 113
Query: 70 A 70
+
Sbjct: 114 S 114
>LON_HELPY (P55995) ATP-dependent protease La (EC 3.4.21.53)
Length = 835
Score = 29.6 bits (65), Expect = 1.3
Identities = 18/61 (29%), Positives = 32/61 (51%), Gaps = 3/61 (4%)
Query: 9 SQNLQRTDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSSSST---SQCDLPKGQGGNV 65
+Q Q+ +S F E+ +KE+ K++DR + +S S++ + D+P GQ G
Sbjct: 250 NQYYQKLESIKPFLKEEAFKEIKKQIDRLSRTHADSSDSATLQNYIETMLDVPFGQYGKK 309
Query: 66 A 66
A
Sbjct: 310 A 310
>CIQ3_RAT (O88944) Potassium voltage-gated channel subfamily KQT
member 3 (Voltage-gated potassium channel subunit Kv7.3)
(Potassium channel alpha subunit KvLQT3) (KQT-like 3)
Length = 873
Score = 29.6 bits (65), Expect = 1.3
Identities = 16/46 (34%), Positives = 23/46 (49%), Gaps = 2/46 (4%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKG--QGGNVAKIKAEHRRVH 76
K + W G LIA++ S S LP G G K++ +HR+ H
Sbjct: 323 KTPKTWEGRLIAATFSLIGVSFFALPAGILGSGLALKVQEQHRQKH 368
>CIQ3_HUMAN (O43525) Potassium voltage-gated channel subfamily KQT
member 3 (Voltage-gated potassium channel subunit Kv7.3)
(Potassium channel alpha subunit KvLQT3) (KQT-like 3)
Length = 872
Score = 29.6 bits (65), Expect = 1.3
Identities = 16/46 (34%), Positives = 23/46 (49%), Gaps = 2/46 (4%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKG--QGGNVAKIKAEHRRVH 76
K + W G LIA++ S S LP G G K++ +HR+ H
Sbjct: 322 KTPKTWEGRLIAATFSLIGVSFFALPAGILGSGLALKVQEQHRQKH 367
>CIQ3_BOVIN (P58126) Potassium voltage-gated channel subfamily KQT
member 3 (Voltage-gated potassium channel subunit Kv7.3)
(Potassium channel alpha subunit KvLQT3) (KQT-like 3)
Length = 866
Score = 29.6 bits (65), Expect = 1.3
Identities = 16/46 (34%), Positives = 23/46 (49%), Gaps = 2/46 (4%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKG--QGGNVAKIKAEHRRVH 76
K + W G LIA++ S S LP G G K++ +HR+ H
Sbjct: 322 KTPKTWEGRLIAATFSLIGVSFFALPAGILGSGLALKVQEQHRQKH 367
>MAP3_SCHPO (P31397) Pheromone M-factor receptor
Length = 365
Score = 29.3 bits (64), Expect = 1.7
Identities = 14/44 (31%), Positives = 26/44 (58%), Gaps = 1/44 (2%)
Query: 6 LTCSQNLQRTDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSS 49
L C ++++ T + G+ ++KE C ++R WS + I S +SS
Sbjct: 314 LDCCKSIESTMA-GKTLDSTDFKEKCLVLERQWSKSSIPSDNSS 356
>SAL2_HUMAN (Q9Y467) Sal-like protein 2 (Zinc finger protein SALL2)
(HSal2)
Length = 1007
Score = 28.9 bits (63), Expect = 2.2
Identities = 26/96 (27%), Positives = 49/96 (50%), Gaps = 13/96 (13%)
Query: 3 LNCLTCSQNLQRTDSFGEFFTEKEYKEVCKKVDRNWS--GNLIA------SSSSSSSTSQ 54
L L+C + L+ +G+ E+ +K CK R +S GNL A +S ++ + +
Sbjct: 637 LRVLSCPRALRL--HYGQHGGERPFK--CKVCGRAFSTRGNLRAHFVGHKASPAARAQNS 692
Query: 55 CDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPK 90
C + + + N ++ +H R+H G IP G++ P+
Sbjct: 693 CPICQKKFTNAVTLQ-QHVRMHLGGQIPNGGTALPE 727
>DXR_PROMA (Q7VB62) 1-deoxy-D-xylulose 5-phosphate reductoisomerase
(EC 1.1.1.267) (DXP reductoisomerase)
(1-deoxyxylulose-5-phosphate reductoisomerase)
Length = 412
Score = 28.9 bits (63), Expect = 2.2
Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Query: 77 STGNIPYPGSSQPKLVRSSGMRRDWSFENLAENQDQSVSCH 117
STG IP PG +L S G RDW+ E+L + + H
Sbjct: 173 STG-IPTPGLRNIQLTASGGAFRDWATEDLKHASIKDATSH 212
>DF1A_ARATH (Q9SEZ3) Dof zinc finger protein DOF1.10 (AtDOF1.10)
(H-protein promoter binding factor-2b)
Length = 399
Score = 28.9 bits (63), Expect = 2.2
Identities = 24/71 (33%), Positives = 34/71 (47%), Gaps = 8/71 (11%)
Query: 35 DRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLV-- 92
D N SG +++ SS SS ++ K Q G+ AKI A+ S N Y G P+++
Sbjct: 202 DNNNSGTILSFGSSESSVTETG--KHQSGDTAKISADS---VSQENKSYQGFLPPQVMLP 256
Query: 93 -RSSGMRRDWS 102
SS WS
Sbjct: 257 NNSSPWPYQWS 267
>DEAD_KLEPN (P33906) Cold-shock DEAD-box protein A (ATP-dependent
RNA helicase deaD)
Length = 642
Score = 28.9 bits (63), Expect = 2.2
Identities = 18/61 (29%), Positives = 27/61 (43%)
Query: 39 SGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLVRSSGMR 98
S I + +S S +LPKG G V + R ++ N+ G +QP+ R G
Sbjct: 516 SSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPRTERRGGGE 575
Query: 99 R 99
R
Sbjct: 576 R 576
>DXR_SYNPX (Q7U8C3) 1-deoxy-D-xylulose 5-phosphate reductoisomerase
(EC 1.1.1.267) (DXP reductoisomerase)
(1-deoxyxylulose-5-phosphate reductoisomerase)
Length = 409
Score = 28.5 bits (62), Expect = 2.8
Identities = 17/37 (45%), Positives = 23/37 (61%), Gaps = 2/37 (5%)
Query: 70 AEHRRVHSTGNIPYPGSSQPKLVRSSGMRRDWSFENL 106
AE+ R+ STG +P PG + +L S G RDW E+L
Sbjct: 164 AENARL-STG-VPTPGLRRIQLTASGGAFRDWKAEDL 198
>DXR_PROMM (Q7V6J8) 1-deoxy-D-xylulose 5-phosphate reductoisomerase
(EC 1.1.1.267) (DXP reductoisomerase)
(1-deoxyxylulose-5-phosphate reductoisomerase)
Length = 412
Score = 28.5 bits (62), Expect = 2.8
Identities = 18/48 (37%), Positives = 26/48 (53%), Gaps = 2/48 (4%)
Query: 70 AEHRRVHSTGNIPYPGSSQPKLVRSSGMRRDWSFENLAENQDQSVSCH 117
AE+ R+ STG +P PG +L S G RDWS ++L + + H
Sbjct: 167 AENARL-STG-VPTPGLRHIQLTASGGAFRDWSAKDLEKATIADATSH 212
>CIQ1_XENLA (P70057) Potassium voltage-gated channel subfamily KQT
member 1 (Voltage-gated potassium channel subunit Kv7.1)
(IKs producing slow voltage-gated potassium channel
alpha subunit xKvLQT1) (KQT-like 1) (Fragment)
Length = 377
Score = 28.1 bits (61), Expect = 3.7
Identities = 19/56 (33%), Positives = 25/56 (43%), Gaps = 2/56 (3%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKG--QGGNVAKIKAEHRRVHSTGNIPYPGS 86
KV + W G IAS S + S LP G G K++ + R+ H IP S
Sbjct: 284 KVPQTWIGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAAS 339
>CIQ1_SQUAC (O73925) Potassium voltage-gated channel subfamily KQT
member 1 (Voltage-gated potassium channel subunit Kv7.1)
(IKs producing slow voltage-gated potassium channel
alpha subunit KvLQT1) (KQT-like 1)
Length = 660
Score = 28.1 bits (61), Expect = 3.7
Identities = 19/56 (33%), Positives = 25/56 (43%), Gaps = 2/56 (3%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKG--QGGNVAKIKAEHRRVHSTGNIPYPGS 86
KV + W G IAS S + S LP G G K++ + R+ H IP S
Sbjct: 311 KVPQTWIGRTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAAS 366
>CIQ1_RAT (Q9Z0N7) Potassium voltage-gated channel subfamily KQT
member 1 (Voltage-gated potassium channel subunit Kv7.1)
(IKs producing slow voltage-gated potassium channel
alpha subunit KvLQT1) (KQT-like 1)
Length = 669
Score = 28.1 bits (61), Expect = 3.7
Identities = 19/56 (33%), Positives = 25/56 (43%), Gaps = 2/56 (3%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKG--QGGNVAKIKAEHRRVHSTGNIPYPGS 86
KV + W G IAS S + S LP G G K++ + R+ H IP S
Sbjct: 318 KVPQTWVGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAAS 373
>CIQ1_MOUSE (P97414) Potassium voltage-gated channel subfamily KQT
member 1 (Voltage-gated potassium channel subunit Kv7.1)
(IKs producing slow voltage-gated potassium channel
alpha subunit KvLQT1) (KQT-like 1)
Length = 668
Score = 28.1 bits (61), Expect = 3.7
Identities = 19/56 (33%), Positives = 25/56 (43%), Gaps = 2/56 (3%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKG--QGGNVAKIKAEHRRVHSTGNIPYPGS 86
KV + W G IAS S + S LP G G K++ + R+ H IP S
Sbjct: 317 KVPQTWVGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAAS 372
>CIQ1_HUMAN (P51787) Potassium voltage-gated channel subfamily KQT
member 1 (Voltage-gated potassium channel subunit Kv7.1)
(IKs producing slow voltage-gated potassium channel
alpha subunit KvLQT1) (KQT-like 1)
Length = 676
Score = 28.1 bits (61), Expect = 3.7
Identities = 19/56 (33%), Positives = 25/56 (43%), Gaps = 2/56 (3%)
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKG--QGGNVAKIKAEHRRVHSTGNIPYPGS 86
KV + W G IAS S + S LP G G K++ + R+ H IP S
Sbjct: 318 KVPQTWVGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAAS 373
>PEX6_PENCH (Q9HG03) Peroxisomal biogenesis factor 6 (Peroxin-6)
Length = 1459
Score = 27.7 bits (60), Expect = 4.9
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 4/44 (9%)
Query: 38 WSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNI 81
W GN + S+S S+TS KG+G +V+K S G++
Sbjct: 1384 WPGNPVRSTSGQSTTSS----KGKGKSVSKKGKSRTGAESDGSV 1423
>LON_HELPJ (Q9ZJL3) ATP-dependent protease La (EC 3.4.21.53)
Length = 831
Score = 27.7 bits (60), Expect = 4.9
Identities = 16/56 (28%), Positives = 30/56 (53%), Gaps = 3/56 (5%)
Query: 9 SQNLQRTDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSSSST---SQCDLPKGQ 61
+Q Q+ +S F E+ +KE+ K++DR + +S S++ + D+P GQ
Sbjct: 250 NQYYQKLESIKPFLKEEAFKEIKKQIDRLSRTHADSSDSATLQNYVETMLDVPFGQ 305
>LMA5_MOUSE (Q61001) Laminin alpha-5 chain precursor
Length = 3718
Score = 27.7 bits (60), Expect = 4.9
Identities = 22/92 (23%), Positives = 35/92 (37%), Gaps = 19/92 (20%)
Query: 1 MSLNCLTCSQNLQRTDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSSSSTSQCDLPKG 60
M L LT LQ D GEF T Y ++C D W +
Sbjct: 3568 MQLKVLTEQVLLQANDGAGEFSTWVTYPKLC---DGRWHRVAVI---------------- 3608
Query: 61 QGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLV 92
G + +++ + + H+TG +P + P L+
Sbjct: 3609 MGRDTLRLEVDTQSNHTTGRLPESLAGSPALL 3640
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.311 0.125 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,153,166
Number of Sequences: 164201
Number of extensions: 524741
Number of successful extensions: 1504
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 1483
Number of HSP's gapped (non-prelim): 45
length of query: 117
length of database: 59,974,054
effective HSP length: 93
effective length of query: 24
effective length of database: 44,703,361
effective search space: 1072880664
effective search space used: 1072880664
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0520.11