BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0490.17
(392 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PGLR_MEDSA (Q40312) Polygalacturonase precursor (EC 3.2.1.15) (P... 494 e-139
PGLR_TOBAC (Q05967) Polygalacturonase precursor (EC 3.2.1.15) (P... 400 e-111
PGLR_GOSHI (Q39786) Polygalacturonase precursor (EC 3.2.1.15) (P... 378 e-104
PGLR_GOSBA (Q39766) Polygalacturonase precursor (EC 3.2.1.15) (P... 377 e-104
PGLR_OENOR (P24548) Exopolygalacturonase (EC 3.2.1.67) (ExoPG) (... 364 e-100
PGL2_PLAAC (Q6H9K0) Exopolygalacturonase precursor (EC 3.2.1.67)... 362 1e-99
PGLR_BRANA (P35337) Polygalacturonase precursor (EC 3.2.1.15) (P... 355 1e-97
PGL2_ARATH (P49063) Exopolygalacturonase clone GBGA483 precursor... 333 4e-91
PGLR_PRUPE (P48979) Polygalacturonase precursor (EC 3.2.1.15) (P... 275 1e-73
PGLR_MAIZE (P26216) Exopolygalacturonase precursor (EC 3.2.1.67)... 271 3e-72
PGLS_MAIZE (P35338) Exopolygalacturonase precursor (EC 3.2.1.67)... 270 4e-72
PGLT_MAIZE (P35339) Exopolygalacturonase precursor (EC 3.2.1.67)... 269 1e-71
PGL1_ARATH (P49062) Exopolygalacturonase clone GBGE184 precursor... 265 2e-70
PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (P... 248 2e-65
PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (P... 237 4e-62
PGL2_CHAOB (Q7M1E7) Polygalacturonase precursor (PG) (EC 3.2.1.1... 233 6e-61
PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (P... 228 2e-59
PGL2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.1... 228 3e-59
PGL2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (P... 218 2e-56
PGLR_LYCES (P05117) Polygalacturonase 2A precursor (EC 3.2.1.15)... 218 3e-56
>PGLR_MEDSA (Q40312) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 421
Score = 494 bits (1271), Expect = e-139
Identities = 243/392 (61%), Positives = 291/392 (73%), Gaps = 9/392 (2%)
Query: 8 STAIVLFLLLLSG--SAQSIVFDIRTFGGAPNSDITQAFLNAWKQACASTSPSKIVISKA 65
STAI++ L ++ +AQS V DI FGG PNSDI QA +AW +ACAST+ +KIVI
Sbjct: 4 STAIIVSFLFIADFCAAQSGVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAG 63
Query: 66 TYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGR 125
TY++N + +KGPCKAPIE+QVDGTIQAP DP+ + G QW Y+D+ TLSG G FDG+
Sbjct: 64 TYQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQ 123
Query: 126 GASAWKLQKACGATCSKRS-----MNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTF 180
GA+ +K A K S MN GFNF+ +S VR VTSKDSK FHV + GC N TF
Sbjct: 124 GATVYKKAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITF 183
Query: 181 DGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPG 240
DGF I APGDS NTDGIH+ KSTDVKILN+NI TGDDCVS+GDG+K IT+ V CGPG
Sbjct: 184 DGFTITAPGDSP--NTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPG 241
Query: 241 HGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVN 300
HG S+GSLG TEE VE I VKNCTLT T NGVRIKTWP APGT V+++ FEDITM N
Sbjct: 242 HGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTN 301
Query: 301 VYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVEL 360
V NPVIIDQ+Y P N CSK++PSKIK+S ++FKN+KGTS T +GV+L CSS VPC+GVEL
Sbjct: 302 VKNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVEL 361
Query: 361 SDISLTFNGAPAMAKCANVKPIIIGKAPSCSA 392
+++ L FNGAP AKC NVKP++ G AP C A
Sbjct: 362 NNVDLKFNGAPTTAKCTNVKPLVTGTAPVCQA 393
>PGLR_TOBAC (Q05967) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 396
Score = 400 bits (1028), Expect = e-111
Identities = 199/397 (50%), Positives = 275/397 (69%), Gaps = 12/397 (3%)
Query: 1 MDMSLIRSTAIVLFLLLLSGSAQSIVFDIRTFGGAPNSDITQAFLNAWKQACASTSPSKI 60
MD+ A+VL L G +Q+ VFDI +G N+DI++A LNA+K+AC STSPS I
Sbjct: 1 MDLKFKVHFALVLLFLAHFGESQTGVFDITKYGANSNADISEALLNAFKEACQSTSPSTI 60
Query: 61 VISKATYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNG 120
VI K T+ MN V ++GPCK+P+E+Q+ T++AP+DP+ L +W+T+ +D FT+SG G
Sbjct: 61 VIPKGTFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVG-EWLTVNKLDQFTMSGGG 119
Query: 121 TFDGRGASAWKLQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTF 180
DG+ A+AW+ +++ C+K N+ FN L +ST++ +T+ DSK FHV + C N TF
Sbjct: 120 ILDGQAAAAWECKQS--KKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTF 177
Query: 181 DGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPG 240
F + AP +S NTDGIH+++S+ V I +SN +TGDDC+S+GD + + I V CGPG
Sbjct: 178 IRFNVSAPANSP--NTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPG 235
Query: 241 HGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTA-PGTSPVTNLTFEDITMV 299
HG S+GSLG NP E+PV + V+NCT TNT NGVRIKTWP + PG V ++ FEDI +
Sbjct: 236 HGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPASHPGV--VNDVHFEDIIVQ 293
Query: 300 NVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVE 359
NV NPV+IDQ YCP N C+K PS++KIS V+F+NIKGTS T+D V L S GVPCEG+E
Sbjct: 294 NVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIE 353
Query: 360 LSDISLTFNG--APAMAKCANVKPIIIGK--APSCSA 392
+ DI +T++G PA + C N+KP + GK P C+A
Sbjct: 354 VGDIDITYSGKEGPAKSSCENIKPSLKGKQNPPVCTA 390
>PGLR_GOSHI (Q39786) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 407
Score = 378 bits (970), Expect = e-104
Identities = 193/394 (48%), Positives = 259/394 (64%), Gaps = 19/394 (4%)
Query: 3 MSLIRSTAIVLFLLLLSGSAQSIVFDIRTFGGAP---NSDITQAFLNAWKQACASTSPSK 59
++++ S ++L L + + QS FD+ GA +D+++ FL+AWK+ACAS +PS
Sbjct: 5 LNIVPSMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPST 64
Query: 60 IVISKATYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGN 119
+VI K TY ++ VN++GPCKAPIEI V GTIQAP DP+A WV V+ F + G
Sbjct: 65 VVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDP-NWVRFYSVENFKMFGG 123
Query: 120 GTFDGRGASAWKLQKACGATC------SKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLL 173
G FDG+G+ A++ TC SK +NI F+FL ++ ++ +TSKDSK FH+ +
Sbjct: 124 GIFDGQGSIAYEKN-----TCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVF 178
Query: 174 GCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIH 233
C N T + KI AP +S NTDGIH+ KS V I+ S+I TGDDC+S+GDG K++ I
Sbjct: 179 ACKNITLERLKIEAPDESP--NTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIK 236
Query: 234 NVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTF 293
+ CGPGHG SIGSLG EEPVE I + NCT+TNT NG RIKTWP G V+ + F
Sbjct: 237 EITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHG-GAVSEIHF 295
Query: 294 EDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGV 353
EDITM NV +P++IDQQYCP N C K SK+K+SN++FKNI+GTS + + CS
Sbjct: 296 EDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSS 355
Query: 354 PCEGVELSDISLTFNGA-PAMAKCANVKPIIIGK 386
PC+ VEL+DI + NGA PA ++C NVKPI GK
Sbjct: 356 PCQNVELADIDIKHNGAEPATSQCLNVKPITSGK 389
>PGLR_GOSBA (Q39766) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 407
Score = 377 bits (968), Expect = e-104
Identities = 192/394 (48%), Positives = 259/394 (65%), Gaps = 19/394 (4%)
Query: 3 MSLIRSTAIVLFLLLLSGSAQSIVFDIRTFGGAP---NSDITQAFLNAWKQACASTSPSK 59
++++ S ++L L + + Q FD+ GA +D+++ FL+AWK+ACAS +PS
Sbjct: 5 LNIVPSMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPST 64
Query: 60 IVISKATYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGN 119
+VI K TY ++ VN++GPCKAPIEI V GTIQAP DP+A WV V+ F + G
Sbjct: 65 VVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDP-NWVRFYSVENFKMFGG 123
Query: 120 GTFDGRGASAWKLQKACGATC------SKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLL 173
G FDG+G+ A++ TC SK +NI F+F+ ++ ++ +TSKDSK FH+ +
Sbjct: 124 GIFDGQGSIAYEKN-----TCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVF 178
Query: 174 GCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIH 233
C N T + KI AP +S NTDGIH+ KS V I+ S+I TGDDC+S+GDG K++ I
Sbjct: 179 ACKNITLERLKIEAPDESP--NTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIK 236
Query: 234 NVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTF 293
+ CGPGHG SIGSLG EEPVE I + NCT+TNT NG RIKTWP G V+ + F
Sbjct: 237 EITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHG-GAVSEIHF 295
Query: 294 EDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGV 353
EDITM NV +P++IDQQYCP N C K SK+K+SN++FKNI+GTS + + CS
Sbjct: 296 EDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSS 355
Query: 354 PCEGVELSDISLTFNGA-PAMAKCANVKPIIIGK 386
PC+ VEL+DI + NGA PA ++C NVKPI IGK
Sbjct: 356 PCQNVELADIDIQHNGAEPATSQCLNVKPITIGK 389
>PGLR_OENOR (P24548) Exopolygalacturonase (EC 3.2.1.67) (ExoPG)
(Pectinase) (Galacturan 1,4-alpha-galacturonidase)
(Fragment)
Length = 362
Score = 364 bits (934), Expect = e-100
Identities = 181/354 (51%), Positives = 240/354 (67%), Gaps = 12/354 (3%)
Query: 39 DITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPNA 98
D TQA AWK+ACAS SPS I++ K + + + ++GPCK+ I +Q+ GT++AP DP+
Sbjct: 1 DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60
Query: 99 LNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQKAC---GATCSKRSMNIGFNFLKHS 155
+ G W+ + +D T+ G G FDG+G SAW +Q C G C SMN+ + +S
Sbjct: 61 IKG-LGWINLNKIDLLTIFGGGVFDGQGKSAW-VQNDCHKNGPICKTLSMNLRLYAVTNS 118
Query: 156 TVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIA 215
+R VT+ DSK FHV ++GC N TF+ FKI A SINTDGIHI +S V I+N+ I
Sbjct: 119 ILRDVTTLDSKNFHVNVIGCKNLTFERFKISAA--ETSINTDGIHIGRSDGVNIINTEIK 176
Query: 216 TGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVR 275
TGDDC+SLGDG+K+I I N+ CGPGHG S+GSLG EE V I VKNCT+T +QNGVR
Sbjct: 177 TGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVR 236
Query: 276 IKTWP-TAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKN 334
IKTWP + PG + + + F+DITM +V P++IDQ YCP N C+ + PS +K+S ++FKN
Sbjct: 237 IKTWPKSEPGEA--SEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKN 294
Query: 335 IKGTSETKDGVILNCSSGVPCEGVELSDISLTFN--GAPAMAKCANVKPIIIGK 386
IKGTS TK+ V L CS PC GVEL+DI LT++ G PA + C N+KP I GK
Sbjct: 295 IKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGK 348
>PGL2_PLAAC (Q6H9K0) Exopolygalacturonase precursor (EC 3.2.1.67)
(Pollen allergen Pla a 2) (Fragment)
Length = 377
Score = 362 bits (928), Expect = 1e-99
Identities = 176/365 (48%), Positives = 240/365 (65%), Gaps = 8/365 (2%)
Query: 26 VFDIRTFGGAPNSDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCK-APIEI 84
VF++ +G DI+QA + AWK ACAS PS ++I K Y M V ++GPCK + I
Sbjct: 9 VFNVNDYGAKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIGF 68
Query: 85 QVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQKAC-GATCSKR 143
Q+DG ++AP DP+ WV+ +D T+SG GT DG+G +AW C
Sbjct: 69 QIDGVVKAPADPSKFKSD-GWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHA 127
Query: 144 SMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAK 203
+MN+ F+FLKH+ VR +TS +SK FH+ +L C + TF + APG SI NTDGIH+
Sbjct: 128 AMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSI--NTDGIHVGI 185
Query: 204 STDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVK 263
S V I N+ IATGDDC+S+G G++++TI V CGPGHG SIGSLG E+ V I VK
Sbjct: 186 SKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVK 245
Query: 264 NCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPS 323
CT + T NGVR+KTWP +P T+LTF+D+TM NV NPVI+DQ+YCP CS+Q+PS
Sbjct: 246 GCTFSGTMNGVRVKTWPNSP-PGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPS 304
Query: 324 KIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGA--PAMAKCANVKP 381
+IK+SN+ F NI+GTS K V++ CS G+PC +++ +I+L++ GA PA + C+NVKP
Sbjct: 305 RIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKP 364
Query: 382 IIIGK 386
GK
Sbjct: 365 TFSGK 369
>PGLR_BRANA (P35337) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 397
Score = 355 bits (910), Expect = 1e-97
Identities = 180/378 (47%), Positives = 245/378 (64%), Gaps = 12/378 (3%)
Query: 12 VLFLLLLSGSAQSIVFDIRTFGGAPNSDITQAFLNAWKQACASTSPSKIVISKATYKMNA 71
+L L LL SA + VF T GG PNSDIT A L A+ AC + +PS+++I K +K+
Sbjct: 10 ILVLCLLGYSANAEVF---TAGGPPNSDITAAVLKAFTSACQAPAPSQVLIPKGDFKLGE 66
Query: 72 VNVKGPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWK 131
+ GPCK+PIE + G ++ TD + G +WV ++ F L+G GTFDG G +AWK
Sbjct: 67 TVMTGPCKSPIEFTLQGNVK--TDGGSTQGKDRWVVFEKINGFKLNGGGTFDGEGNAAWK 124
Query: 132 LQKACGAT--CSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPG 189
C T C K +++ F+F+ ++ ++ VTS D+K FH ++ N TFD KIIAP
Sbjct: 125 ANN-CHKTFECKKLPISVRFDFVDNAEIKDVTSLDAKNFHFNVISGKNMTFDNIKIIAPA 183
Query: 190 DSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLG 249
+S NTDGIH+ + VKILN+ IATGDDC+S+GDG K++ I V CGPGHG S+GSLG
Sbjct: 184 ESP--NTDGIHLGRCEGVKILNTKIATGDDCISVGDGMKNLLIEKVVCGPGHGISVGSLG 241
Query: 250 TNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQ 309
E+ V +I VKNCTL T NG+RIKTWP+A T+ + FEDI + V NP++IDQ
Sbjct: 242 RYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAACTTTAAGIHFEDIILNKVSNPILIDQ 301
Query: 310 QYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNG 369
+YCP N C+K PS IK+ ++TF+NI+GTSE KD V L CS G PCE VE+ DI++ + G
Sbjct: 302 EYCPWNQCNKNKPSTIKLVDITFRNIRGTSENKDAVKLLCSKGHPCENVEIGDINIEYTG 361
Query: 370 --APAMAKCANVKPIIIG 385
P +C NV P ++G
Sbjct: 362 PDGPPTFECTNVTPKLVG 379
>PGL2_ARATH (P49063) Exopolygalacturonase clone GBGA483 precursor
(EC 3.2.1.67) (ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 444
Score = 333 bits (854), Expect = 4e-91
Identities = 171/376 (45%), Positives = 235/376 (62%), Gaps = 13/376 (3%)
Query: 18 LSGSAQSIVFDIRTFG--GAPNSDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVK 75
+SG+ D++ G G +D + AF AWK+ACA+ S I + K Y + ++ K
Sbjct: 61 VSGAKPGAAVDVKASGAKGDSKTDDSAAFAAAWKEACAAGST--ITVPKGEYMVESLEFK 118
Query: 76 GPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGN-GTFDGRGASAWKLQK 134
GPCK P+ ++++G +AP W+ + FTL+GN FDG+G+ AWK
Sbjct: 119 GPCKGPVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKAND 178
Query: 135 ACGAT--CSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSI 192
C T C+ +NI F L +S + S+TS +SK FH+ +L C N T I AP +S+
Sbjct: 179 -CAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESL 237
Query: 193 SINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNP 252
NTDGIHI +S V ++ + I TGDDCVS+GDG +++ + NV+CGPGHG SIGSLG P
Sbjct: 238 --NTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYP 295
Query: 253 TEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYC 312
E+PV+ + V+ C + NT NGVRIKTWP +P +N+ FEDITM NV PV+IDQ+YC
Sbjct: 296 NEQPVKGVTVRKCLIKNTDNGVRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYC 354
Query: 313 PSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNG--A 370
P C PS++K+S+VT K IKGTS TK V L CS GVPC + LSDI+L NG
Sbjct: 355 PYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEG 414
Query: 371 PAMAKCANVKPIIIGK 386
PA++ C+N+KPI+ GK
Sbjct: 415 PAVSACSNIKPILSGK 430
>PGLR_PRUPE (P48979) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 393
Score = 275 bits (704), Expect = 1e-73
Identities = 143/374 (38%), Positives = 216/374 (57%), Gaps = 7/374 (1%)
Query: 8 STAIVLFLLLLSGSAQSIVFDIRTFGGAPN--SDITQAFLNAWKQACASTSPSKIVISKA 65
S +++ ++ S A + +++ + G + +D T+AFL+AW +ACAS +P I +
Sbjct: 9 SLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAG 68
Query: 66 TYKMNAVNVKGPCKA-PIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDG 124
T+ + V GPCK I ++ GT+ AP+D + A W+ +V+ T+SG G DG
Sbjct: 69 TFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISG-GILDG 127
Query: 125 RGASAWKLQKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFK 184
+G + W + G +C + +GF+ + V + S +S+ FH+ + N G +
Sbjct: 128 QGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVR 187
Query: 185 IIAPGDSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFS 244
+ G+S NTDGIH+ S+ V ILNS IATGDDCVS+G G ++ I V CGPGHG S
Sbjct: 188 VSRSGNSP--NTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGIS 245
Query: 245 IGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNP 304
IGSLG E V+N+ VK T + TQNG+RIK+W P T N+ F+ TMVNV NP
Sbjct: 246 IGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSW-GRPSTGFARNILFQHATMVNVENP 304
Query: 305 VIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDIS 364
++IDQ YCP N S ++IS+VT+++I GTS T+ V +CS PC ++L D+
Sbjct: 305 IVIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVK 364
Query: 365 LTFNGAPAMAKCAN 378
LT+ A + C++
Sbjct: 365 LTYKNQAAESSCSH 378
>PGLR_MAIZE (P26216) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 271 bits (692), Expect = 3e-72
Identities = 142/360 (39%), Positives = 201/360 (55%), Gaps = 9/360 (2%)
Query: 27 FDIRTFGGAPN--SDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIEI 84
FDI G + N +D T+A AW AC T I+I K + + +N GPCK + I
Sbjct: 41 FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTI 100
Query: 85 QVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQKACGAT--CSK 142
QVDG + A TD + W+ I VD ++G G DG+G + W + +C C
Sbjct: 101 QVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWS-KNSCTKKYDCKI 159
Query: 143 RSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIA 202
++ +F+ + V VT +SKFFH+ + C + + APGDS NTDGIH+
Sbjct: 160 LPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSP--NTDGIHMG 217
Query: 203 KSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIV 262
S+ + I N+ I GDDC+S+G G + I V CGPGHG SIGSLG E+ V +I V
Sbjct: 218 DSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINV 277
Query: 263 KNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSP 322
K+CTL T GVRIK + A V+ + +E+I M + NP+ ID +YCP+ LC+
Sbjct: 278 KDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGA 337
Query: 323 SKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGA--PAMAKCANVK 380
SK+ + +VTFKNI GTS T + V L C++ VPC GV + D+++ ++G MA C N K
Sbjct: 338 SKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNAK 397
>PGLS_MAIZE (P35338) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 270 bits (691), Expect = 4e-72
Identities = 141/360 (39%), Positives = 200/360 (55%), Gaps = 9/360 (2%)
Query: 27 FDIRTFGGAPN--SDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIEI 84
FDI G + N +D T+A AW AC T I+I K + + +N GPCK + I
Sbjct: 41 FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTI 100
Query: 85 QVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQKACGAT--CSK 142
QVDG + A TD + W+ I VD ++G G DG+G + W + +C C
Sbjct: 101 QVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWS-KNSCTKKYDCKI 159
Query: 143 RSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIA 202
++ +F+ + V VT +SKFFH+ + C N + APGDS NTDGIH+
Sbjct: 160 LPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSP--NTDGIHMG 217
Query: 203 KSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIV 262
S+ + I N+ I GDDC+S+G G + I V CGPGHG SIGSLG E+ V +I V
Sbjct: 218 DSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINV 277
Query: 263 KNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSP 322
K+CTL T GVRIK + A V+ + +E+I M + NP+ ID +YCP+ LC+
Sbjct: 278 KDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGA 337
Query: 323 SKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGA--PAMAKCANVK 380
SK+ + +VTFKNI GTS T + + L C++ VPC G + D+++ ++G MA C N K
Sbjct: 338 SKVTVKDVTFKNITGTSSTPEAISLLCTAKVPCTGATMDDVNVEYSGTNNKTMAICTNAK 397
>PGLT_MAIZE (P35339) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 269 bits (687), Expect = 1e-71
Identities = 142/369 (38%), Positives = 201/369 (53%), Gaps = 11/369 (2%)
Query: 27 FDIRTFGGAPN--SDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIEI 84
FDI G + N +D T+A AW AC T I+I K + + +N GPCK + I
Sbjct: 41 FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGDVTI 100
Query: 85 QVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQKACGA-TCSKR 143
QV+G + A TD + W+ I VD ++G G DG+G + W C
Sbjct: 101 QVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCKIL 160
Query: 144 SMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAK 203
++ +F+ + V +T +SKFFH+ + C + + APGDS NTDGIH+
Sbjct: 161 PNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSP--NTDGIHMGD 218
Query: 204 STDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVK 263
S+ V I N+ I GDDC+S+G G + I V CGPGHG SIGSLG E+ V +I VK
Sbjct: 219 SSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 278
Query: 264 NCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPS 323
+CTL T NGVRIK + A + + +E+I M + P+IID +YCP+ LC+ S
Sbjct: 279 DCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGAS 338
Query: 324 KIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGA--PAMAKCANVKP 381
K+ + +VTFKNI GTS T + V L C++ +PC GV + D+++ ++G MA C N K
Sbjct: 339 KVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAK- 397
Query: 382 IIIGKAPSC 390
G A C
Sbjct: 398 ---GSAKGC 403
>PGL1_ARATH (P49062) Exopolygalacturonase clone GBGE184 precursor
(EC 3.2.1.67) (ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 422
Score = 265 bits (677), Expect = 2e-70
Identities = 153/391 (39%), Positives = 217/391 (55%), Gaps = 29/391 (7%)
Query: 17 LLSGSAQSIVFDIRTFG--GAPNSDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNV 74
L + S V+DI FG G +++ +AFLN W Q C S P+ +++ K T+ V
Sbjct: 42 LAANSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIF 101
Query: 75 KGPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQK 134
GPCK+ + + V GTI A T A +W VD L+G GTF G+G + WK
Sbjct: 102 AGPCKSKVTVNVIGTIIATTSGYATP---EWFLFERVDNLVLTGTGTFHGKGEAVWKAD- 157
Query: 135 ACG--ATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSI 192
CG C+ ++ F +K+ + ++S ++K FH+ L+ N K+ AP +S
Sbjct: 158 GCGKKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESP 217
Query: 193 SINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNP 252
NTDGIH++ + +V IL+S IATGDDCVS+G G+ ++T+ V CGPGHG S+GSLG
Sbjct: 218 --NTDGIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYK 275
Query: 253 TEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYC 312
EE V I V NCT+ T NG+RIKTW S ++ FE+I M +V NP+IIDQ Y
Sbjct: 276 NEEDVSGIHVNNCTMIETDNGLRIKTW-GGSDPSKAVDIKFENIIMQSVKNPIIIDQNY- 333
Query: 313 PSNLCSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTF----- 367
S+ S++ IS++ FKNI+GT+ TKD V + CS VPC+GV + D++L +
Sbjct: 334 ----GSRGGDSQVAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTG 389
Query: 368 ------NGAPAMAKCANVKPIIIGKA--PSC 390
+G A C N I GK P C
Sbjct: 390 GEKKSSSGGLVGALCDNANVIFGGKLSFPMC 420
>PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 460
Score = 248 bits (632), Expect = 2e-65
Identities = 137/383 (35%), Positives = 208/383 (53%), Gaps = 20/383 (5%)
Query: 17 LLSGSAQSIVFDIRTFGGAPN-SDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVK 75
+ + SA + + FG N +D TQAF+ AWK AC+S+ +V+ + Y + +
Sbjct: 89 IATSSAPAKTISVDDFGAKGNGADDTQAFVKAWKAACSSSGAMVLVVPQKNYLVRPIEFS 148
Query: 76 GPCKAPIEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKL--- 132
GPCK+ + +Q+ GTI+A D + W+ V + G GT +G G WK
Sbjct: 149 GPCKSQLTLQIYGTIEASEDRSIYKDIDHWLIFDNVQNLLVVGPGTINGNGNIWWKNSCK 208
Query: 133 ---QKACGATCSKRSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPG 189
Q CG + FN + V+++ +D++ HV C N + AP
Sbjct: 209 IKPQPPCGTYAPTA---VTFNRCNNLVVKNLNIQDAQQIHVIFQNCINVQASCLTVTAPE 265
Query: 190 DSISINTDGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLG 249
DS NTDGIH+ + ++ I +S I TGDDC+S+ G++ + ++ CGPGHG SIGSLG
Sbjct: 266 DSP--NTDGIHVTNTQNITISSSVIGTGDDCISIVSGSQRVQATDITCGPGHGISIGSLG 323
Query: 250 TNPTEEPVENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQ 309
+ +E+ V + V L+ T NG+RIKTW G++ TN+ F+++ M +V NP+IIDQ
Sbjct: 324 EDGSEDHVSGVFVNGAKLSGTSNGLRIKTWKGGSGSA--TNIVFQNVQMNDVTNPIIIDQ 381
Query: 310 QYCPSNLCS-KQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFN 368
YC KQ S +++ NV ++NI+GTS + D + LNCS VPC+G+ L + L N
Sbjct: 382 NYCDHKTKDCKQQKSAVQVKNVLYQNIRGTSASGDAITLNCSQSVPCQGIVLQSVQLQ-N 440
Query: 369 GAPAMAKCANVKPIIIG-KAPSC 390
G A+C NV+P G +P C
Sbjct: 441 G---RAECNNVQPAYKGVVSPRC 460
>PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 467
Score = 237 bits (604), Expect = 4e-62
Identities = 123/348 (35%), Positives = 195/348 (55%), Gaps = 12/348 (3%)
Query: 39 DITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPNA 98
D T+AF AWK AC+STS + +++ K Y + ++ GPCK+ + +Q+ GTI+A D +
Sbjct: 103 DDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTMQIYGTIEASDDRSD 162
Query: 99 LN-GAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQKACGAT----CSKRSMNIGFNFLK 153
W+ V + G GT +G G W Q +C C + F K
Sbjct: 163 YRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWW--QNSCKTNKALPCKDAPTALTFYKSK 220
Query: 154 HSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSN 213
H V+++ ++++ HV+ C N + AP +S NTDGIH+ + ++ I +
Sbjct: 221 HVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSP--NTDGIHVTGTQNIHISSCV 278
Query: 214 IATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNG 273
I TGDDC+S+ +G++ + ++++ CGPGHG SIGSLG +E V +++V L T NG
Sbjct: 279 IGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLCGTTNG 338
Query: 274 VRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFK 333
VRIKTW G++ +N+ F+++ M NV NP+IIDQ YC + ++ S +++ NV ++
Sbjct: 339 VRIKTWQGGSGSA--SNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQ 396
Query: 334 NIKGTSETKDGVILNCSSGVPCEGVELSDISLTF-NGAPAMAKCANVK 380
NIKGT + + +CS PC+G+ L D+ L GA A A C NV+
Sbjct: 397 NIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVE 444
>PGL2_CHAOB (Q7M1E7) Polygalacturonase precursor (PG) (EC 3.2.1.15)
(Pectinase) (Major pollen allergen Cha o 2)
Length = 514
Score = 233 bits (594), Expect = 6e-61
Identities = 137/374 (36%), Positives = 200/374 (52%), Gaps = 13/374 (3%)
Query: 26 VFDIRTFG--GAPNSDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIE 83
VF++ +G G D T+AF W AC S +V + + +N + +GPC+ +
Sbjct: 58 VFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLS 117
Query: 84 IQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAW--KLQKACGAT-C 140
+VDGTI A DP + W+ + F L G G DG+G W + + G T C
Sbjct: 118 FKVDGTIVAQPDPARWKNSKIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKVVNGRTVC 177
Query: 141 SKRSMN--IGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDG 198
+ R+ I ++ K TV+ +T +S FH+ C G KI AP DS NTDG
Sbjct: 178 NDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSP--NTDG 235
Query: 199 IHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVE 258
I I S I I TGDDC+++G G+ +ITI ++ CGPGHG SIGSLG + + V
Sbjct: 236 IDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSRAEVS 295
Query: 259 NIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCS 318
++ V +TQNG+RIKTW G + + +T+E++ M+N NP++I+Q YC S
Sbjct: 296 HVHVNRAKFIDTQNGLRIKTWQGGSGLA--SYITYENVEMINSENPILINQFYCTSASAC 353
Query: 319 KQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTF-NGAPAMAKCA 377
+ S ++I VT+KNI GTS T + L CS VPC G++LS++SL +G PA
Sbjct: 354 QNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPASCVDK 413
Query: 378 NVKPIIIGK-APSC 390
N + G+ P+C
Sbjct: 414 NARGFYSGRLIPTC 427
>PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 462
Score = 228 bits (581), Expect = 2e-59
Identities = 126/347 (36%), Positives = 189/347 (54%), Gaps = 10/347 (2%)
Query: 38 SDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPN 97
+D T+AF AWK AC+S S IV Y + + GPCK+ + +++ GTI+A +D +
Sbjct: 97 TDDTKAFEKAWKDACSSGSVL-IVPENKNYLLKQITFSGPCKSDLRVKIRGTIEASSDQS 155
Query: 98 ALNG--AFQWVTIGYVDYFTLSGNGTFDGRGASAW--KLQKACGATCSKRSMNIGFNFLK 153
G +W+ + TL G GT +G G + W ++ C + F K
Sbjct: 156 DWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCKRKKSLPCKSAPTALTFRSCK 215
Query: 154 HSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSN 213
+ V ++ KDS+ H++ C + + AP S NTDGIHI + + ++NS
Sbjct: 216 NLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSP--NTDGIHITGTQRIHVMNSV 273
Query: 214 IATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNG 273
I TGDDC+S+ G+K + N+ CGPGHG SIGSLG +E V ++V L +T NG
Sbjct: 274 IGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNG 333
Query: 274 VRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFK 333
+RIKTW G++ N+ F++I M NV NP+IIDQ YC S + S +K+SNV +
Sbjct: 334 LRIKTWQGGSGSA--KNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYM 391
Query: 334 NIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGA-PAMAKCANV 379
NI+GTS ++ V +CS PC+G + +I+L NG C+N+
Sbjct: 392 NIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKETTMSCSNI 438
>PGL2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.15)
(Pectinase) (Major pollen allergen Jun a 2)
Length = 507
Score = 228 bits (580), Expect = 3e-59
Identities = 136/362 (37%), Positives = 187/362 (51%), Gaps = 12/362 (3%)
Query: 24 SIVFDIRTFG--GAPNSDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAP 81
+IVF++ +G G D T AF W AC S +V + + +N + GPC+
Sbjct: 57 AIVFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANKKFVVNNLVFYGPCQPH 116
Query: 82 IEIQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQKAC--GAT 139
+VDGTI A DP + W+ + F L G G DG+G W Q G T
Sbjct: 117 FSFKVDGTIAAYPDPAKWKNSKIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKTINGRT 176
Query: 140 CSK---RSMNIGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINT 196
R I +F K TV+ +T +S FH+ C G KI AP DS NT
Sbjct: 177 VCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDSP--NT 234
Query: 197 DGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEP 256
DGI I S +I I TGDDCV++G G+ +ITI ++ CGPGHG SIGSLG +
Sbjct: 235 DGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNSRSE 294
Query: 257 VENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNL 316
V + + +TQNG+RIKTW G + +++T+E++ M+N NP++I+Q YC S
Sbjct: 295 VSFVHLDGAKFIDTQNGLRIKTWQGGSGLA--SHITYENVEMINAENPILINQFYCTSAA 352
Query: 317 CSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGAPAMAKC 376
K S +KI +VTFKNI GTS T + L CS VPC ++LS++ L +A C
Sbjct: 353 ACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSVPCSNIKLSNVFLKLTSG-KVATC 411
Query: 377 AN 378
N
Sbjct: 412 VN 413
>PGL2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase) (Major pollen allergen Cry j 2) (Cry j II)
Length = 514
Score = 218 bits (556), Expect = 2e-56
Identities = 126/362 (34%), Positives = 186/362 (50%), Gaps = 16/362 (4%)
Query: 26 VFDIRTFG--GAPNSDITQAFLNAWKQACASTSPSKIVISKATYKMNAVNVKGPCKAPIE 83
+F++ +G G D T+AF AW+ AC S +V + +N + GPC+
Sbjct: 58 IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117
Query: 84 IQVDGTIQAPTDPNALNGAFQWVTIGYVDYFTLSGNGTFDGRGASAWKLQKACGATCSKR 143
+VDG I A +P + W+ + FTL G G DG+G W Q C +
Sbjct: 118 FKVDGIIAAYQNPASWKNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQ--CKWVNGRE 175
Query: 144 SMN-------IGFNFLKHSTVRSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINT 196
N I F+F ++ + +S FH+ C G I AP DS NT
Sbjct: 176 ICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSP--NT 233
Query: 197 DGIHIAKSTDVKILNSNIATGDDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEP 256
DGI I S + + + I TGDDCV++G G+ +I I ++ CGPGHG SIGSLG +
Sbjct: 234 DGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAE 293
Query: 257 VENIIVKNCTLTNTQNGVRIKTWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNL 316
V + V +TQNG+RIKTW G+ +++ +E++ M+N NP++I+Q YC S
Sbjct: 294 VSYVHVNGAKFIDTQNGLRIKTW--QGGSGMASHIIYENVEMINSENPILINQFYCTSAS 351
Query: 317 CSKQSPSKIKISNVTFKNIKGTSETKDGVILNCSSGVPCEGVELSDISLTFNGAPAMAKC 376
+ S ++I +VT+KNI+GTS T + L CS +PC+ ++LSDISL +A C
Sbjct: 352 ACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLTSG-KIASC 410
Query: 377 AN 378
N
Sbjct: 411 LN 412
>PGLR_LYCES (P05117) Polygalacturonase 2A precursor (EC 3.2.1.15)
(PG-2A) (Pectinase)
Length = 457
Score = 218 bits (554), Expect = 3e-56
Identities = 117/343 (34%), Positives = 188/343 (54%), Gaps = 12/343 (3%)
Query: 43 AFLNAWKQACASTSPSKIVISK-ATYKMNAVNVKGPCKAPIEIQVDGTIQAPTDPNALNG 101
AF AW +AC+S +P + V+ K Y + + GPC++ I +++ G+++A + +
Sbjct: 94 AFEQAWNEACSSRTPVQFVVPKNKNYLLKQITFSGPCRSSISVKIFGSLEASSKISDYKD 153
Query: 102 AFQWVTIGYVDYFTLSGNGTFDGRGASAW----KLQKACGATCSKRSMNIGFNFLKHSTV 157
W+ V + G GT +G G W K+ K+ C + F K+ V
Sbjct: 154 RRLWIAFDSVQNLVVGGGGTINGNGQVWWPSSCKINKSL--PCRDAPTALTFWNCKNLKV 211
Query: 158 RSVTSKDSKFFHVTLLGCTNFTFDGFKIIAPGDSISINTDGIHIAKSTDVKILNSNIATG 217
++ SK+++ H+ CTN I A S NTDG+H++ + ++I ++ I TG
Sbjct: 212 NNLKSKNAQQIHIKFESCTNVVASNLMINASAKSP--NTDGVHVSNTQYIQISDTIIGTG 269
Query: 218 DDCVSLGDGNKHITIHNVKCGPGHGFSIGSLGTNPTEEPVENIIVKNCTLTNTQNGVRIK 277
DDC+S+ G++++ N+ CGPGHG SIGSLG+ +E V N+ V + +NGVRIK
Sbjct: 270 DDCISIVSGSQNVQATNITCGPGHGISIGSLGSGNSEAYVSNVTVNEAKIIGAENGVRIK 329
Query: 278 TWPTAPGTSPVTNLTFEDITMVNVYNPVIIDQQYCPSNLCSKQSPSKIKISNVTFKNIKG 337
TW G+ +N+ F ++ M +V P+IIDQ YC Q S +++ NV ++NIKG
Sbjct: 330 TW--QGGSGQASNIKFLNVEMQDVKYPIIIDQNYCDRVEPCIQQFSAVQVKNVVYENIKG 387
Query: 338 TSETKDGVILNCSSGVPCEGVELSDISLT-FNGAPAMAKCANV 379
TS TK + +CS+ PCEG+ + +I+L +G P+ A C NV
Sbjct: 388 TSATKVAIKFDCSTNFPCEGIIMENINLVGESGKPSEATCKNV 430
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,453,803
Number of Sequences: 164201
Number of extensions: 2160600
Number of successful extensions: 4588
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 4386
Number of HSP's gapped (non-prelim): 57
length of query: 392
length of database: 59,974,054
effective HSP length: 112
effective length of query: 280
effective length of database: 41,583,542
effective search space: 11643391760
effective search space used: 11643391760
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 67 (30.4 bits)
Lotus: description of TM0490.17