BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0487.13
(394 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CCA4_DROME (Q9VE00) Probable cytochrome P450 12a4, mitochondrial... 35 0.25
TLR4_PONPY (Q8SPE9) Toll-like receptor 4 precursor 35 0.43
POLG_BVDVN (P19711) Genome polyprotein [Contains: N-terminal pro... 35 0.43
TLR4_PAPAN (Q9TSP2) Toll-like receptor 4 precursor 34 0.74
TLR4_PANPA (Q9TTN0) Toll-like receptor 4 precursor 34 0.74
TLR4_HUMAN (O00206) Toll-like receptor 4 precursor (hToll) 34 0.74
G3P_METMA (Q8PTD3) Glyceraldehyde-3-phosphate dehydrogenase (EC ... 34 0.74
NT56_DROME (Q27333) Lethal(2)neighbour of tid protein (NOT56) (N... 33 1.6
POLG_BVDVC (Q96662) Genome polyprotein [Contains: N-terminal pro... 32 2.2
G3P2_METAC (P58838) Glyceraldehyde-3-phosphate dehydrogenase 2 (... 31 4.8
G3P1_METAC (P58837) Glyceraldehyde-3-phosphate dehydrogenase 1 (... 31 4.8
EX5C_BUCAI (P57528) Exodeoxyribonuclease V gamma chain (EC 3.1.1... 31 4.8
YJO9_YEAST (P47005) F-box protein YJL149w 31 6.3
Y397_MYCGE (P47637) Hypothetical protein MG397 31 6.3
YABD_BACSU (P37545) Putative deoxyribonuclease yabD (EC 3.1.21.-) 30 8.2
GLMS_METTH (O26273) Glucosamine--fructose-6-phosphate aminotrans... 30 8.2
>CCA4_DROME (Q9VE00) Probable cytochrome P450 12a4, mitochondrial
precursor (EC 1.14.-.-) (CYPXIIA4)
Length = 536
Score = 35.4 bits (80), Expect = 0.25
Identities = 29/119 (24%), Positives = 52/119 (43%), Gaps = 2/119 (1%)
Query: 57 LYGGDEVYARFVEAVYKVILARDFNQPIKKFRLVLTERLKDPANISVWVNLVLKRRVEHI 116
LY +E F + V VI + +P FR V+ L P N+ ++ + + E +
Sbjct: 129 LYHREEYRKDFYQGVMGVIPTQ--GKPWGDFRTVVNPVLMQPKNVRLYYKKMSQVNQEFV 186
Query: 117 DISLHFEDDEFLELPHIVVDTPSMFSCTTLVVLKLEGLELKANFSSVDLPFLKVLHLQD 175
L D + LE P +DT + ++ ++ V+ L+ S+ + LK+ H D
Sbjct: 187 QRILELRDPDTLEAPDDFIDTINRWTLESVSVVALDKQLGLLKNSNKESEALKLFHYLD 245
>TLR4_PONPY (Q8SPE9) Toll-like receptor 4 precursor
Length = 828
Score = 34.7 bits (78), Expect = 0.43
Identities = 42/131 (32%), Positives = 60/131 (45%), Gaps = 24/131 (18%)
Query: 145 TLVVLKLEGLELKAN-----FSSVDLPFLKVLHLQDLLLQDEGCLAEILSGCLALE--DL 197
TL + L+ L AN FS VDLP L+ L L L +GC ++ G +L+ DL
Sbjct: 345 TLELKSLKRLTFTANKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDL 404
Query: 198 KARDVFFDGNKADAEFITLPKLVRADISESGGSQHFMMKVVNNVS-FLRIHEIDYNLIYM 256
DV G + F+ L +L D QH +K ++ S FL + NLIY+
Sbjct: 405 SFNDVITMG----SNFLGLEQLEHLDF------QHSNLKQMSEFSVFLSLR----NLIYL 450
Query: 257 GEDMFHNLTHL 267
D+ H T +
Sbjct: 451 --DISHTHTRV 459
>POLG_BVDVN (P19711) Genome polyprotein [Contains: N-terminal protease
(EC 3.4.22.-) (N-pro) (Autoprotease p20); Capsid protein
C; E(rns) glycoprotein (gp44/48); Envelope glycoprotein
E1 (gp33); Envelope glycoprotein E2 (gp55); Protein P7;
Nonstructural p
Length = 3988
Score = 34.7 bits (78), Expect = 0.43
Identities = 30/120 (25%), Positives = 59/120 (49%), Gaps = 19/120 (15%)
Query: 24 LLTTKEAVTTSILSKRWIPLWRSVPTLDF-------DDSNLYGGDEVYARFVEAVYKV-- 74
LL +A S +S +W ++ S TLDF + GG + +R V A+ ++
Sbjct: 1361 LLPLIKATLISCVSSKWQLIYMSYLTLDFMYYMHRKVIEEISGGTNIISRLVAALIELNW 1420
Query: 75 ILARDFNQPIKKFRLVLTERLKDPANISVWVNLVLKRRVEHIDISLHFEDDEFLELPHIV 134
+ + ++ +KKF L L+ RL+ NL++K +V + ++ + ++E +P I+
Sbjct: 1421 SMEEEESKGLKKFYL-LSGRLR---------NLIIKHKVRNETVASWYGEEEVYGMPKIM 1470
>TLR4_PAPAN (Q9TSP2) Toll-like receptor 4 precursor
Length = 826
Score = 33.9 bits (76), Expect = 0.74
Identities = 42/129 (32%), Positives = 59/129 (45%), Gaps = 24/129 (18%)
Query: 145 TLVVLKLEGLELKAN-----FSSVDLPFLKVLHLQDLLLQDEGCLAEILSGCLALE--DL 197
TL + L+ L AN FS VDLP L+ L L L +GC ++ G +L+ DL
Sbjct: 347 TLELESLKRLTFTANKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDL 406
Query: 198 KARDVFFDGNKADAEFITLPKLVRADISESGGSQHFMMKVVNNVS-FLRIHEIDYNLIYM 256
DV G + F+ L +L D QH +K ++ S FL + NLIY+
Sbjct: 407 SFNDVITMG----SNFLGLEQLEHLDF------QHSNLKQMSQFSVFLSLR----NLIYL 452
Query: 257 GEDMFHNLT 265
D+ H T
Sbjct: 453 --DISHTHT 459
>TLR4_PANPA (Q9TTN0) Toll-like receptor 4 precursor
Length = 839
Score = 33.9 bits (76), Expect = 0.74
Identities = 38/135 (28%), Positives = 62/135 (45%), Gaps = 14/135 (10%)
Query: 145 TLVVLKLEGLELKAN-----FSSVDLPFLKVLHLQDLLLQDEGCLAEILSGCLALEDLKA 199
TL + L+ L +N FS VDLP L+ L L L +GC ++ G +L+ L
Sbjct: 347 TLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYL-- 404
Query: 200 RDVFFDG-NKADAEFITLPKLVRADISESGGSQHFMMKV---VNNVSFLRIHEIDYNLIY 255
D+ F+G + F+ L +L D S Q V + N+ +L I + +
Sbjct: 405 -DLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAF 463
Query: 256 MGEDMFHNLTHLELV 270
G +F+ L+ LE++
Sbjct: 464 NG--IFNGLSSLEVL 476
>TLR4_HUMAN (O00206) Toll-like receptor 4 precursor (hToll)
Length = 839
Score = 33.9 bits (76), Expect = 0.74
Identities = 38/135 (28%), Positives = 62/135 (45%), Gaps = 14/135 (10%)
Query: 145 TLVVLKLEGLELKAN-----FSSVDLPFLKVLHLQDLLLQDEGCLAEILSGCLALEDLKA 199
TL + L+ L +N FS VDLP L+ L L L +GC ++ G +L+ L
Sbjct: 347 TLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYL-- 404
Query: 200 RDVFFDG-NKADAEFITLPKLVRADISESGGSQHFMMKV---VNNVSFLRIHEIDYNLIY 255
D+ F+G + F+ L +L D S Q V + N+ +L I + +
Sbjct: 405 -DLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAF 463
Query: 256 MGEDMFHNLTHLELV 270
G +F+ L+ LE++
Sbjct: 464 NG--IFNGLSSLEVL 476
>G3P_METMA (Q8PTD3) Glyceraldehyde-3-phosphate dehydrogenase (EC
1.2.1.59) (GAPDH) (NAD(P)-dependent
glyceraldehyde-3-phosphate dehydrogenase)
Length = 335
Score = 33.9 bits (76), Expect = 0.74
Identities = 26/94 (27%), Positives = 45/94 (47%), Gaps = 4/94 (4%)
Query: 73 KVILARDFNQPIKKFRLVLTERLKDPANISVW-VNLVLKRRVEHIDISLHFEDDEFLELP 131
+VI D +KK R+ L R DP +I +N ++ + I + H D LP
Sbjct: 148 RVIYPIDRELGVKKVRVTLARRATDPNDIKKGPINAIVP---DPIKLPSHHGPDIKSVLP 204
Query: 132 HIVVDTPSMFSCTTLVVLKLEGLELKANFSSVDL 165
HI + T ++ TTL+ L +E+ + ++ D+
Sbjct: 205 HINITTAALKIPTTLMHLHTVNMEVNTDCTAEDI 238
>NT56_DROME (Q27333) Lethal(2)neighbour of tid protein (NOT56)
(NOT45)
Length = 510
Score = 32.7 bits (73), Expect = 1.6
Identities = 26/85 (30%), Positives = 39/85 (45%), Gaps = 7/85 (8%)
Query: 300 QFYNVYLNKLGAKDWQYPSSVP-ECILLHLKECCLNHYRGT---KGELQFAKYIMEHG-- 353
QFY Y + L W P S+ C++L L E C N Y T L F I+ G
Sbjct: 426 QFYVWYFHSLPYLAWSTPYSLGVRCLILGLIEYCWNTYPSTNFSSAALHFTHIILLAGVA 485
Query: 354 -RLLNKMTICSSTAEKQGEKLENLK 377
+L+ M I ++ +Q E+ + L+
Sbjct: 486 KQLIQTMRINNAAKREQQEQQKKLQ 510
>POLG_BVDVC (Q96662) Genome polyprotein [Contains: N-terminal protease
(EC 3.4.22.-) (N-pro) (Autoprotease p20); Capsid protein
C; E(rns) glycoprotein (gp44/48); Envelope glycoprotein
E1 (gp33); Envelope glycoprotein E2 (gp55); Protein P7;
Nonstructural p
Length = 3907
Score = 32.3 bits (72), Expect = 2.2
Identities = 28/124 (22%), Positives = 59/124 (47%), Gaps = 19/124 (15%)
Query: 20 HILSLLTTKEAVTTSILSKRWIPLWRSVPTLDF-------DDSNLYGGDEVYARFVEAVY 72
+I LL A S +S +W ++ + TLDF + G V +R + A+
Sbjct: 1366 NISILLPLIRATLISCISSKWQMVYMAYLTLDFMYYMHRKVIEEISGSTNVMSRVIAALI 1425
Query: 73 KV--ILARDFNQPIKKFRLVLTERLKDPANISVWVNLVLKRRVEHIDISLHFEDDEFLEL 130
++ + + ++ +KKF +L+ RL+ NL++K +V + ++ + ++E +
Sbjct: 1426 ELNWSMEEEESKGLKKF-FILSGRLR---------NLIIKHKVRNQTVASWYGEEEVYGM 1475
Query: 131 PHIV 134
P +V
Sbjct: 1476 PKVV 1479
>G3P2_METAC (P58838) Glyceraldehyde-3-phosphate dehydrogenase 2 (EC
1.2.1.59) (GAPDH 2) (NAD(P)-dependent
glyceraldehyde-3-phosphate dehydrogenase 2)
Length = 335
Score = 31.2 bits (69), Expect = 4.8
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 4/82 (4%)
Query: 85 KKFRLVLTERLKDPANISVW-VNLVLKRRVEHIDISLHFEDDEFLELPHIVVDTPSMFSC 143
KK R+ L R DP +I +N ++ + I + H D +PHI + + +M
Sbjct: 160 KKVRVTLARRATDPNDIKKGPINAIVP---DPIKLPSHHGPDVKTVIPHIDITSAAMKIP 216
Query: 144 TTLVVLKLEGLELKANFSSVDL 165
TTL+ + +ELK ++ D+
Sbjct: 217 TTLMHVHTVNMELKNECTAEDV 238
>G3P1_METAC (P58837) Glyceraldehyde-3-phosphate dehydrogenase 1 (EC
1.2.1.59) (GAPDH 1) (NAD(P)-dependent
glyceraldehyde-3-phosphate dehydrogenase 1)
Length = 335
Score = 31.2 bits (69), Expect = 4.8
Identities = 21/83 (25%), Positives = 41/83 (49%), Gaps = 4/83 (4%)
Query: 84 IKKFRLVLTERLKDPANISVW-VNLVLKRRVEHIDISLHFEDDEFLELPHIVVDTPSMFS 142
+KK R +L R DP +I +N ++ V+ + H D +PHI + + ++
Sbjct: 159 VKKVRAILARRATDPNDIKKGPINAIVLHPVK---LPSHHGPDVRSVIPHINITSAALLV 215
Query: 143 CTTLVVLKLEGLELKANFSSVDL 165
TTL+ L +E+ + ++ D+
Sbjct: 216 PTTLMHLHTVNMEVDTDCTAEDI 238
>EX5C_BUCAI (P57528) Exodeoxyribonuclease V gamma chain (EC
3.1.11.5)
Length = 1070
Score = 31.2 bits (69), Expect = 4.8
Identities = 19/70 (27%), Positives = 31/70 (44%), Gaps = 8/70 (11%)
Query: 259 DMFHNLTHLELVYTTFNRDWFEVLE---FLKYCPKLEVLVIKQPQFYNVYLNK-----LG 310
D F N T +E + W E+++ Y K+ + ++K+ FY Y N G
Sbjct: 552 DFFQNNTKIEKSIQIIQKKWIEIIDDSLSSNYLKKISINILKKIFFYKYYDNNHEIFLPG 611
Query: 311 AKDWQYPSSV 320
++ YP SV
Sbjct: 612 VVNFCYPDSV 621
>YJO9_YEAST (P47005) F-box protein YJL149w
Length = 663
Score = 30.8 bits (68), Expect = 6.3
Identities = 35/117 (29%), Positives = 53/117 (44%), Gaps = 12/117 (10%)
Query: 83 PIKKFRLVLTERLKDPANISVWVNLVLKRRVEHIDISLHFEDDEFLEL------PHIVVD 136
P+ K V +R I W + +LKR E + FE+ E L L HI+VD
Sbjct: 289 PLTKLSEVFDKRTLKILTIISWNDTLLKRETEMLKDFKEFENLEDLSLISIKQDVHILVD 348
Query: 137 TPSMFSCTTLVVLKLEGLELKANFSSVDLPFLKVL----HLQDLLLQDEGCLAEILS 189
S S T L LK++ LE + FL +L LQ + L+ +G + +I++
Sbjct: 349 LFS--SLTNLKRLKMDFLEEYVPEPTNPHIFLSILLACSKLQFIDLRYDGLIPQIIN 403
>Y397_MYCGE (P47637) Hypothetical protein MG397
Length = 566
Score = 30.8 bits (68), Expect = 6.3
Identities = 32/136 (23%), Positives = 58/136 (42%), Gaps = 24/136 (17%)
Query: 167 FLKVLHLQDLLLQDEGCLAEILSGCLALEDLKARDVFFDG--NKADAEFITLPKLVRADI 224
+L VL+ LL ++ + C+ +D++ + F + N IT + DI
Sbjct: 171 YLNVLNRISLLFKE-------IENCIQAKDVEKQIAFLNKLHNILSQTIITANRQYMFDI 223
Query: 225 SESGGSQHFMM-------------KVVNNVSFLRIHEIDYNLIYMGEDMFHNLTHLELVY 271
S + HF + KV+N+ L++HE+ N+ +D + L +L L+
Sbjct: 224 KLSYLTHHFKVLIQKVKQMELMKNKVINSQDLLKLHELINNITIKLKDCTNFLNNLNLML 283
Query: 272 TTFNRDWFEVLEFLKY 287
N D EV E + +
Sbjct: 284 CERNMD--EVQEAISW 297
>YABD_BACSU (P37545) Putative deoxyribonuclease yabD (EC 3.1.21.-)
Length = 255
Score = 30.4 bits (67), Expect = 8.2
Identities = 32/131 (24%), Positives = 50/131 (37%), Gaps = 14/131 (10%)
Query: 260 MFHNLTHLELVYTTFNRDWFEVLEFLKYCPKLEVLVIKQPQFYNVYLN-------KLGAK 312
+ HN E V T + E + + +C V ++ N YL+ K K
Sbjct: 126 IIHNRDATEDVVTILKEEGAEAVGGIMHCFTGSAEVARECMKMNFYLSFGGPVTFKNAKK 185
Query: 313 DWQYPSSVPECILLHLKECCL---NHYRGTKGELQFAKYIMEHGRLLNKMTICSSTAEKQ 369
+ +P LL +C + +RG + E + KY+ E L +MT E
Sbjct: 186 PKEVVKEIPNDRLLIETDCPFLTPHPFRGKRNEPSYVKYVAEQIAELKEMTF----EEIA 241
Query: 370 GEKLENLKKLF 380
EN K+LF
Sbjct: 242 SITTENAKRLF 252
>GLMS_METTH (O26273) Glucosamine--fructose-6-phosphate
aminotransferase [isomerizing] (EC 2.6.1.16)
(Hexosephosphate aminotransferase)
(D-fructose-6-phosphate amidotransferase) (GFAT)
(L-glutamine-D-fructose-6-phosphate amidotransferase)
(Glucosamine-6-ph
Length = 589
Score = 30.4 bits (67), Expect = 8.2
Identities = 23/80 (28%), Positives = 42/80 (51%), Gaps = 11/80 (13%)
Query: 160 FSSVDLPFLKVLHLQDLLLQDEGCLAEILSGCLALEDLKARDVFFDGNKADAEFITLPKL 219
F + D+P + + H ++ D+G + IL G L + D+ +GN + E ++
Sbjct: 189 FLASDVPAI-LNHTSRVIYLDDGEMV-ILDGDLRVTDM-------EGNTVEKEVHSIDW- 238
Query: 220 VRADISESGGSQHFMMKVVN 239
AD++E GG HFM+K ++
Sbjct: 239 -SADMAEKGGYDHFMLKEIH 257
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.140 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,292,091
Number of Sequences: 164201
Number of extensions: 1958872
Number of successful extensions: 5418
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 5415
Number of HSP's gapped (non-prelim): 16
length of query: 394
length of database: 59,974,054
effective HSP length: 112
effective length of query: 282
effective length of database: 41,583,542
effective search space: 11726558844
effective search space used: 11726558844
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 67 (30.4 bits)
Lotus: description of TM0487.13