BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0476b.3
(862 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TRA6_STAAU (P19775) Transposase for insertion sequence element I... 38 0.10
TRA6_ENTFA (P59787) Transposase for insertion sequence element I... 38 0.10
TRA1_LACHE (P35880) Transposase for insertion sequence element I... 37 0.29
TRA6_BURCE (P24575) Transposase for insertion sequence element I... 35 0.65
VGLD_BHV1S (Q08100) Glycoprotein D precursor (Glycoprotein IV) 34 1.9
VGLD_BHV1P (P24906) Glycoprotein D precursor (Glycoprotein IV) 33 2.5
TP3A_HUMAN (Q13472) DNA topoisomerase III alpha (EC 5.99.1.2) 33 2.5
MTLD_THETN (Q8RCS0) Mannitol-1-phosphate 5-dehydrogenase (EC 1.1... 33 3.2
APC_HUMAN (P25054) Adenomatous polyposis coli protein (APC protein) 32 7.2
YURO_BACSU (O32156) Hypothetical ABC transporter extracellular b... 32 9.4
LFTR_DEIRA (Q9RTP4) Leucyl/phenylalanyl-tRNA--protein transferas... 32 9.4
>TRA6_STAAU (P19775) Transposase for insertion sequence element
IS256 in transposon Tn4001
Length = 390
Score = 38.1 bits (87), Expect = 0.10
Identities = 34/127 (26%), Positives = 51/127 (39%), Gaps = 12/127 (9%)
Query: 407 LAFDATYKKNKYRRPLVIFSC-----VNHRNQTTVVGGAVIGNEKEETYVWLLEQLLEAM 461
L D Y K + ++ SC + ++G + E EET+ E L E
Sbjct: 164 LMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKER- 222
Query: 462 DKKSPTTVITDGDKAMRNAIRRVFPNASHRLCAWHLMCNATDNIKIPDFLPKFKTCMFGD 521
+ VI+D K + +AIR+ F N S + C H + N I PK + F +
Sbjct: 223 GLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTI------PKKNSKSFRE 276
Query: 522 FQIGDFK 528
G FK
Sbjct: 277 AVKGIFK 283
>TRA6_ENTFA (P59787) Transposase for insertion sequence element
IS256 in transposon Tn4001
Length = 390
Score = 38.1 bits (87), Expect = 0.10
Identities = 34/127 (26%), Positives = 51/127 (39%), Gaps = 12/127 (9%)
Query: 407 LAFDATYKKNKYRRPLVIFSC-----VNHRNQTTVVGGAVIGNEKEETYVWLLEQLLEAM 461
L D Y K + ++ SC + ++G + E EET+ E L E
Sbjct: 164 LMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKER- 222
Query: 462 DKKSPTTVITDGDKAMRNAIRRVFPNASHRLCAWHLMCNATDNIKIPDFLPKFKTCMFGD 521
+ VI+D K + +AIR+ F N S + C H + N I PK + F +
Sbjct: 223 GLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTI------PKKNSKSFRE 276
Query: 522 FQIGDFK 528
G FK
Sbjct: 277 AVKGIFK 283
>TRA1_LACHE (P35880) Transposase for insertion sequence element
IS1201
Length = 369
Score = 36.6 bits (83), Expect = 0.29
Identities = 24/104 (23%), Positives = 47/104 (45%), Gaps = 5/104 (4%)
Query: 410 DATY---KKNKYRRPLVIFSC-VNHRNQTTVVGGAVIGNEKEETYVWLLEQLLEAMDKKS 465
DATY ++ + R V + + V+ + NE E + LL Q +++ +
Sbjct: 163 DATYVPLRRETFEREAVYIAIGIKPNGHKEVIDYCIAPNENIEVWTELL-QSMKSRGLEQ 221
Query: 466 PTTVITDGDKAMRNAIRRVFPNASHRLCAWHLMCNATDNIKIPD 509
++DG M+ A+ + +P A + C H+M N +++ D
Sbjct: 222 VELFLSDGVVGMKTALAKTYPQAHFQRCLVHVMRNICAKVRVED 265
>TRA6_BURCE (P24575) Transposase for insertion sequence element
IS406
Length = 388
Score = 35.4 bits (80), Expect = 0.65
Identities = 30/100 (30%), Positives = 44/100 (44%), Gaps = 6/100 (6%)
Query: 407 LAFDATYKKNKYRRPLV-----IFSCVNHRNQTTVVGGAVIGNEKEETYVWLLEQLLEAM 461
L DA Y+K + +V I + + V+G V +E E + LE LL A
Sbjct: 163 LFLDARYEKVRLEGRIVDCAVLIAVGIEASGKRRVLGCEVATSEAEINWRRFLESLL-AR 221
Query: 462 DKKSPTTVITDGDKAMRNAIRRVFPNASHRLCAWHLMCNA 501
K T +I D ++ A R V P+ + C +HL NA
Sbjct: 222 GLKGVTLIIADDHAGLKAARRAVLPSVPWQRCQFHLQQNA 261
>VGLD_BHV1S (Q08100) Glycoprotein D precursor (Glycoprotein IV)
Length = 417
Score = 33.9 bits (76), Expect = 1.9
Identities = 26/85 (30%), Positives = 35/85 (40%), Gaps = 14/85 (16%)
Query: 714 IAVLVDLNIHNIPKSLVLERWSQGAKDHLCETPGSSPSLF---RDSAVLVRYVCMLEACR 770
+ V VD + +P+ ERW T G PS F R+ V VRY AC
Sbjct: 26 VTVYVDPPAYPMPRYNYTERW---------HTTGPIPSPFADGREQPVEVRYAASAAACD 76
Query: 771 KMCTLACPLIDDFRQTWEIVGGHTQ 795
+ +A P + R WE V H +
Sbjct: 77 MLALIADPQVG--RTLWEAVRRHAR 99
>VGLD_BHV1P (P24906) Glycoprotein D precursor (Glycoprotein IV)
Length = 417
Score = 33.5 bits (75), Expect = 2.5
Identities = 26/85 (30%), Positives = 35/85 (40%), Gaps = 14/85 (16%)
Query: 714 IAVLVDLNIHNIPKSLVLERWSQGAKDHLCETPGSSPSLF---RDSAVLVRYVCMLEACR 770
+ V VD + +P+ ERW T G PS F R+ V VRY AC
Sbjct: 26 VTVYVDPPAYPMPRYNYTERW---------HTTGPIPSPFADGREQPVEVRYATSAAACD 76
Query: 771 KMCTLACPLIDDFRQTWEIVGGHTQ 795
+ +A P + R WE V H +
Sbjct: 77 MLALIADPQVG--RTLWEAVRRHAR 99
>TP3A_HUMAN (Q13472) DNA topoisomerase III alpha (EC 5.99.1.2)
Length = 1001
Score = 33.5 bits (75), Expect = 2.5
Identities = 21/68 (30%), Positives = 27/68 (38%), Gaps = 1/68 (1%)
Query: 792 GHTQHLENKNNPSVPEGVTGTPSANAGLTTHRRRGRPCGPSTSR-LRAKRPLKCSTCKVI 850
G Q ++ P + T L + R RP S+ AK+P KCS C
Sbjct: 931 GFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQP 990
Query: 851 GHNRLTCP 858
GH R CP
Sbjct: 991 GHTRPFCP 998
>MTLD_THETN (Q8RCS0) Mannitol-1-phosphate 5-dehydrogenase (EC
1.1.1.17)
Length = 386
Score = 33.1 bits (74), Expect = 3.2
Identities = 29/111 (26%), Positives = 47/111 (42%), Gaps = 16/111 (14%)
Query: 378 HTEDADKNLEHLFWCD----GIGRMNYSVFGDALAFDATYKKNKYRRPLVIFSCVNHRNQ 433
H E+ DK L+ D +G N S G L +K K +PL I +C N
Sbjct: 68 HLEEEDKVLDAFLEADMVTTSVGVSNLSSIGGRLKKYLKARKEKNEKPLDIMACENALFA 127
Query: 434 TTVVGGAVIGNEKEETYVWL----------LEQLLEA--MDKKSPTTVITD 472
T V+ V+ E ++ +L +++++ A +DKK P V +
Sbjct: 128 TDVLRERVVKEEDDDFITYLNLKVGFPNTAVDRIVPAVKIDKKLPVDVAVE 178
>APC_HUMAN (P25054) Adenomatous polyposis coli protein (APC protein)
Length = 2843
Score = 32.0 bits (71), Expect = 7.2
Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 6/47 (12%)
Query: 810 TGTPSANAGLTTHRRRGRPCGPSTSRLRAKRPLKCSTCKVIGHNRLT 856
T TPS+NA +R PS+++ R+ +P K +TCKV N+ T
Sbjct: 1218 TSTPSSNA------KRQNQLHPSSAQSRSGQPQKAATCKVSSINQET 1258
>YURO_BACSU (O32156) Hypothetical ABC transporter extracellular
binding protein yurO precursor
Length = 422
Score = 31.6 bits (70), Expect = 9.4
Identities = 22/100 (22%), Positives = 45/100 (45%), Gaps = 7/100 (7%)
Query: 526 DFKRKWESLVE-EFNLHENPWIIDMYARRHTWAAAYFRGKFFAGF-RTTSRCEGFNAQLG 583
D + W+ L++ L E+ + + + TW +++ G +R + +NA+ G
Sbjct: 165 DPPKTWDELIDVSKKLKEHGYTPISFGTKATWTISHYIGTLNQRMVDEKTREKDYNAKTG 224
Query: 584 KFVKRRY-----NLKEFLQHFHHCLEYMRHREVEADFRSG 618
+F Y L+E + +F+ + + H V F+SG
Sbjct: 225 EFTDEGYVKALEKLQELMPYFNKHVNSVDHEYVRQQFKSG 264
>LFTR_DEIRA (Q9RTP4) Leucyl/phenylalanyl-tRNA--protein transferase
(EC 2.3.2.6) (L/F-transferase) (Leucyltransferase)
(Phenyalanyltransferase)
Length = 210
Score = 31.6 bits (70), Expect = 9.4
Identities = 22/77 (28%), Positives = 32/77 (40%), Gaps = 8/77 (10%)
Query: 619 YGDPGLKAPPIFQSIELSASRILTRQLFFRFRPTIVSAADMIITNCEDTLGMSMHTVKRS 678
YG PG P+ + L +R + R+L FRP++ A D +I C L S
Sbjct: 42 YGVPGRALVPLTEDAGLHVARRMRREL-KHFRPSVDLAFDEVIAGCRGHL-------PGS 93
Query: 679 QESSREWLVSYYTTTYE 695
E EW+ Y+
Sbjct: 94 PERDGEWISDELVEIYQ 110
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.351 0.156 0.561
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,632,668
Number of Sequences: 164201
Number of extensions: 3919682
Number of successful extensions: 15535
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 15529
Number of HSP's gapped (non-prelim): 14
length of query: 862
length of database: 59,974,054
effective HSP length: 119
effective length of query: 743
effective length of database: 40,434,135
effective search space: 30042562305
effective search space used: 30042562305
T: 11
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.9 bits)
S2: 70 (31.6 bits)
Lotus: description of TM0476b.3