BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0476a.9
(361 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
APG_ARATH (P40602) Anter-specific proline-rich protein APG precu... 155 2e-37
APG_BRANA (P40603) Anter-specific proline-rich protein APG (Prot... 138 2e-32
EST_HEVBR (Q7Y1X1) Esterase precursor (EC 3.1.1.-) (Early nodule... 73 1e-12
YE07_MYCPN (P75377) Hypothetical protein MPN407 (F11_orf879) 33 1.5
PPSA_METJA (Q57962) Probable phosphoenolpyruvate synthase (EC 2.... 32 2.5
NUSB_SYNEL (Q8DKS0) N utilization substance protein B homolog (N... 32 2.5
NOG2_NEUCR (Q7SHR8) Nucleolar GTP-binding protein 2 32 3.3
YG1W_YEAST (P53230) Hypothetical 44.2 kDa protein in RME1-TFC4 i... 31 5.6
POLG_CXA24 (P36290) Genome polyprotein [Contains: Coat protein V... 31 5.6
UBPD_YEAST (P38187) Ubiquitin carboxyl-terminal hydrolase 13 (EC... 30 9.5
NOG2_PNECA (Q9C3Z4) Nucleolar GTP-binding protein 2 (Binding-ind... 30 9.5
>APG_ARATH (P40602) Anter-specific proline-rich protein APG
precursor
Length = 534
Score = 155 bits (391), Expect = 2e-37
Identities = 99/319 (31%), Positives = 164/319 (51%), Gaps = 26/319 (8%)
Query: 33 VPCIFVFGDSLSDSGNNNNLPTSAKANFLPYGIDFPATFPTGRYSNGRNPIDKIAQMLGF 92
+P +F FGDS+ D+GNNNNL T K+N+ PYG+DF TGR+SNG D +A+ +G
Sbjct: 202 IPAVFFFGDSVFDTGNNNNLETKIKSNYRPYGMDFKFRVATGRFSNGMVASDYLAKYMGV 261
Query: 93 QEFIPPFAN--LNGSDILKGVNYASGSAGIRKESGSQLGHNVNLGLQLLHHRAIVSRI-- 148
+E +P + + + +D+L GV++ASG AG + S+ + + + QL + + + ++
Sbjct: 262 KEIVPAYLDPKIQPNDLLTGVSFASGGAGYNPTT-SEAANAIPMLDQLTYFQDYIEKVNR 320
Query: 149 -------AHRLGGLDKATQYLNQCLYYVNIGTNDFEQNYFLPNVFNTSRMYTPKQYAKAL 201
++L GL+K Q +++ + V G+ND YF Y +
Sbjct: 321 LVRQEKSQYKLAGLEKTNQLISKGVAIVVGGSNDLIITYFGSGAQRLKN--DIDSYTTII 378
Query: 202 IQQLSHYLQTLHHFGARKSVLVGLDRLGCIP--KLLVNGSCVEEKNVATFLFNDQLKSLV 259
+ ++ L+ +GAR+ ++G LGC+P +L C EE N A+ LFN +L ++
Sbjct: 379 ADSAASFVLQLYGYGARRIGVIGTPPLGCVPSQRLKKKKICNEELNYASQLFNSKLLLIL 438
Query: 260 DRLNKKILMDSKFIFINSTAIIHD-----KSVGFTVTHHGCCPTN--EKGKCIRDGIP-- 310
+L+ K L +S F++++ II + GF T CC T G +
Sbjct: 439 GQLS-KTLPNSTFVYMDIYTIISQMLETPAAYGFEETKKPCCKTGLLSAGALCKKSTSKI 497
Query: 311 CQNRHEYVFWDGIHTTEAA 329
C N Y+FWDG+H T+ A
Sbjct: 498 CPNTSSYLFWDGVHPTQRA 516
>APG_BRANA (P40603) Anter-specific proline-rich protein APG (Protein
CEX) (Fragment)
Length = 449
Score = 138 bits (347), Expect = 2e-32
Identities = 96/320 (30%), Positives = 152/320 (47%), Gaps = 32/320 (10%)
Query: 33 VPCIFVFGDSLSDSGNNNNLPTSAKANFLPYGIDFPATFPTGRYSNGRNPIDKIAQMLGF 92
+P +F FGDS+ D+GNNNNL T K N+ PYG+DFP TGR+SNGR D I++ LG
Sbjct: 123 IPAVFFFGDSIFDTGNNNNLDTKLKCNYRPYGMDFPMGVATGRFSNGRVASDYISKYLGV 182
Query: 93 QEFIPPFAN--------LNGSDILKGVNYASGSAGIRKESGSQLGHNVNLGLQLLHHRAI 144
+E +P + + L SD+L GV++ASG AG ++ V L L +
Sbjct: 183 KEIVPAYVDKKLQQNNELQQSDLLTGVSFASGGAGYLPQTSE--SWKVTTMLDQLTYFQD 240
Query: 145 VSRIAHRLGGLDKATQYLNQCLYYVNIGTNDFEQNYFLPNVFNTSRMYTPKQYAKALIQQ 204
+ +L G K + +++ V G+ND YF + + +
Sbjct: 241 YKKRMKKLVGKKKTKKIVSKGAAIVVAGSNDLIYTYFGNGAQHLKN--DVDSFTTMMADS 298
Query: 205 LSHYLQTLHHFGARKSVLVGLDRLGCIPKLLVNGS--CVEEKNVATFLFNDQLKSLVDRL 262
+ ++ L+ +GAR+ ++G +GC P V C E+ N A LFN +L ++ +L
Sbjct: 299 AASFVLQLYGYGARRIGVIGTPPIGCTPSQRVKKKKICNEDLNYAAQLFNSKLVIILGQL 358
Query: 263 NKKILMDSKFIF-----INSTAIIHDKSVGFTVTHHGCCPTNEKGKCIRDGIPCQ----- 312
+ K L +S ++ I S + + GF CC K + G+ C+
Sbjct: 359 S-KTLPNSTIVYGDIYSIFSKMLESPEDYGFEEIKKPCC----KIGLTKGGVFCKERTLK 413
Query: 313 ---NRHEYVFWDGIHTTEAA 329
N Y+FWDG+H ++ A
Sbjct: 414 NMSNASSYLFWDGLHPSQRA 433
>EST_HEVBR (Q7Y1X1) Esterase precursor (EC 3.1.1.-) (Early
nodule-specific protein homolog) (Latex allergen Hev b
13)
Length = 391
Score = 73.2 bits (178), Expect = 1e-12
Identities = 90/365 (24%), Positives = 142/365 (38%), Gaps = 63/365 (17%)
Query: 9 LVLSLLVVMVASIMQHSTVLGESQVPCIFVFGDSLSDSGNNNNLPTSAKANFLPYGIDFP 68
+ LS L+ M++ T P IF FGDS SD+G A F P +
Sbjct: 12 ITLSFLLCMLSLAYASETC----DFPAIFNFGDSNSDTGGK-------AAAFYPLNPPYG 60
Query: 69 ATF---PTGRYSNGRNPIDKIAQMLGFQEFIPPFANLNGSDILKGVNYASGSAGIRKESG 125
TF TGRYS+GR ID IA+ ++ P+ + GS+ G ++A+ + I+ +
Sbjct: 61 ETFFHRSTGRYSDGRLIIDFIAESFNL-PYLSPYLSSLGSNFKHGADFATAGSTIKLPTT 119
Query: 126 SQLGHN----VNLGLQLLHHRAIV--SRIAHRLGG----LDKATQYLNQCLYYVNIGTND 175
H L +Q R + S+ GG L Y + LY +IG ND
Sbjct: 120 IIPAHGGFSPFYLDVQYSQFRQFIPRSQFIRETGGIFAELVPEEYYFEKALYTFDIGQND 179
Query: 176 FEQNYFLPNVFNTSRMYTPKQYAKALIQQLSHYLQTLHHFGARKSVLVGLDRLGCIPKLL 235
+ + V + L+ S ++ ++ GAR + +GC+ +L
Sbjct: 180 LTEGFLNLTVEEVNAT------VPDLVNSFSANVKKIYDLGARTFWIHNTGPIGCLSFIL 233
Query: 236 V--------NGSCVEEKNVATFLFNDQLKSLVDRLNKKILMDSKFIFINSTAIIHD---- 283
+ C + N FN +LK +V +L K + + + F+ ++ ++ +
Sbjct: 234 TYFPWAEKDSAGCAKAYNEVAQHFNHKLKEIVAQLRKDLPL-ATFVHVDIYSVKYSLFSE 292
Query: 284 -KSVGFTVTHHGCC-----------------PTNEKGKCIRDGIPCQNRHEYVFWDGIHT 325
+ GF CC T + G I G C V WDG H
Sbjct: 293 PEKHGFEFPLITCCGYGGKYNFSVTAPCGDTVTADDGTKIVVG-SCACPSVRVNWDGAHY 351
Query: 326 TEAAN 330
TEAAN
Sbjct: 352 TEAAN 356
>YE07_MYCPN (P75377) Hypothetical protein MPN407 (F11_orf879)
Length = 879
Score = 32.7 bits (73), Expect = 1.5
Identities = 41/150 (27%), Positives = 59/150 (39%), Gaps = 39/150 (26%)
Query: 129 GHNVNLGLQLLHHRAIVSRIAHRLGGLDKATQYLNQCLYYVNIGTNDFEQNYFLPNVFNT 188
G N +L L + +HR QY N +Y +N+GTND Q
Sbjct: 586 GSNTSLELYMQNHRF---------------KQYKNIDVYVLNLGTNDINQ--------LA 622
Query: 189 SRMYTPKQYAKALIQQLSHYLQTLHHFGARKSVLVGLDRLGCIPKLLVNGSCVEEKNVAT 248
+YT +QY L+ L LH + V++ P L+ S EKN T
Sbjct: 623 QGVYTVEQYK----HNLTQILDLLHTQSPQAHVVLA----NIAPSSLLRSS---EKNWKT 671
Query: 249 FLFNDQLKSLVDRLNKKI---LMDSKFIFI 275
FND L+ + N L+D K +F+
Sbjct: 672 --FNDFLEGIPKDSNYSSFVHLLDQKSLFL 699
>PPSA_METJA (Q57962) Probable phosphoenolpyruvate synthase (EC
2.7.9.2) (Pyruvate, water dikinase) (PEP synthase)
[Contains: Mja pep intein (Mja pepA intein)]
Length = 1188
Score = 32.0 bits (71), Expect = 2.5
Identities = 20/60 (33%), Positives = 33/60 (54%), Gaps = 6/60 (10%)
Query: 210 QTLHHFGARKSVLVGLDRLGCIPKLLVNGSCVEEKNVATFLFNDQLKSLVDRLNKKILMD 269
Q H + ++VGL RLG IP+L S + AT FN+ L++++ R ++I +D
Sbjct: 650 QNSSHIKKIEGLIVGLYRLGIIPRLRYKRS-----STATIYFNNNLETILQR-TRRIKLD 703
>NUSB_SYNEL (Q8DKS0) N utilization substance protein B homolog (NusB
protein)
Length = 212
Score = 32.0 bits (71), Expect = 2.5
Identities = 25/108 (23%), Positives = 43/108 (39%)
Query: 135 GLQLLHHRAIVSRIAHRLGGLDKATQYLNQCLYYVNIGTNDFEQNYFLPNVFNTSRMYTP 194
G +L + ++ +L L+KA L L G N LP + +
Sbjct: 54 GTELSESQRLLRHSEFQLPHLEKAQMVLQDALQRAEKGLNKIGAALDLPEILYFAHQEEV 113
Query: 195 KQYAKALIQQLSHYLQTLHHFGARKSVLVGLDRLGCIPKLLVNGSCVE 242
+ YA AL++ L Y + R V L+RL + + ++ + VE
Sbjct: 114 QAYAIALLRCLQRYQNEIEELVNRSMVDWQLERLTQLDRAIIEIAVVE 161
>NOG2_NEUCR (Q7SHR8) Nucleolar GTP-binding protein 2
Length = 619
Score = 31.6 bits (70), Expect = 3.3
Identities = 23/100 (23%), Positives = 42/100 (42%), Gaps = 1/100 (1%)
Query: 161 YLNQCLYYVNIGTNDFEQNYFLPNVFNTSRMYTPKQYAKALIQQLS-HYLQTLHHFGARK 219
YL C V ND Q+ L V + P+QY A++ ++ H+++ + K
Sbjct: 373 YLIDCPGIVPPNQNDTPQDLLLRGVVRVENVDNPEQYIPAVLNKVKPHHMERTYELKGWK 432
Query: 220 SVLVGLDRLGCIPKLLVNGSCVEEKNVATFLFNDQLKSLV 259
+ L+ L L+ G + VA + ND ++ +
Sbjct: 433 DHIHFLEMLARKGGRLLKGGEPDVDGVAKMVLNDFMRGKI 472
>YG1W_YEAST (P53230) Hypothetical 44.2 kDa protein in RME1-TFC4
intergenic region
Length = 385
Score = 30.8 bits (68), Expect = 5.6
Identities = 25/98 (25%), Positives = 44/98 (44%), Gaps = 15/98 (15%)
Query: 27 VLGESQVPCIFVFG--DSLSDSGNNNNLPTSAKANFLPYGIDFPATFPT-------GRYS 77
V+ + PC FVFG + + + + + + + G+ +P+ F + YS
Sbjct: 116 VMSSFKAPCRFVFGYGSGVFEQAGYSKSHSKPQIDII-LGVTYPSHFHSINMRQNPQHYS 174
Query: 78 N----GRNPIDKIAQMLGFQEFIPPFANLNGSDILKGV 111
+ G + K Q +G + PFAN+NG D+ GV
Sbjct: 175 SLKYFGSEFVSKF-QQIGAGVYFNPFANINGHDVKYGV 211
>POLG_CXA24 (P36290) Genome polyprotein [Contains: Coat protein VP4
(P1A); Coat protein VP2 (P1B); Coat protein VP3 (P1C);
Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29) (Core
protein P2A); Core protein P2B; Core protein P2C; Core
protein P3A; Genome
Length = 2214
Score = 30.8 bits (68), Expect = 5.6
Identities = 22/88 (25%), Positives = 34/88 (38%), Gaps = 3/88 (3%)
Query: 81 NPIDKIAQMLGFQEFIPPFANLNGSDILKGVNYASGSAGIRKESGSQLGHNVNLGLQLLH 140
N + QM+ EFIPP A+L IL +Y S + + H+ L +
Sbjct: 1317 NDMKLFCQMVSTVEFIPPMASLEEKGILFTSDYVLASTNSHTITPPTVSHSDALNRRFAF 1376
Query: 141 HRAIVSRIAHRLGG---LDKATQYLNQC 165
+ + H + G + ATQ C
Sbjct: 1377 DMEVYTMSEHSIKGKLNMATATQLCKDC 1404
>UBPD_YEAST (P38187) Ubiquitin carboxyl-terminal hydrolase 13 (EC
3.1.2.15) (Ubiquitin thiolesterase 13)
(Ubiquitin-specific processing protease 13)
(Deubiquitinating enzyme 13)
Length = 688
Score = 30.0 bits (66), Expect = 9.5
Identities = 35/142 (24%), Positives = 58/142 (40%), Gaps = 20/142 (14%)
Query: 86 IAQMLGFQEFIPPFANLNGSDILKGVNYASGSAGI------RKESGSQLGHNVNLGLQLL 139
+ ++L ++ +N N D LKG + +S S + R S SQ+ L+
Sbjct: 312 VGRVLNYENPSRGSSNSNNLD-LKGESNSSLSTPLDKKDTRRSSSSSQISPEHRKKSALI 370
Query: 140 HHRAIVSRIAHRLGGLDKATQYLNQCLYYVNIGTNDFEQNYFLPN-----------VFNT 188
R V I H L G DKAT Y + + I N + P+ +FNT
Sbjct: 371 --RGPVLNIDHSLNGSDKATLYSSLRDIFECITENTYLTGVVSPSSFVDVLKRENVLFNT 428
Query: 189 SRMYTPKQYAKALIQQLSHYLQ 210
+ ++ L+ +LS Y++
Sbjct: 429 TMHQDAHEFFNFLLNELSEYIE 450
>NOG2_PNECA (Q9C3Z4) Nucleolar GTP-binding protein 2
(Binding-inducible GTPase)
Length = 483
Score = 30.0 bits (66), Expect = 9.5
Identities = 20/101 (19%), Positives = 41/101 (39%), Gaps = 2/101 (1%)
Query: 161 YLNQCLYYVNIGTNDFEQNYFLPNVFNTSRMYTPKQYAKALIQ--QLSHYLQTLHHFGAR 218
++ C V +ND E + ++ P+QY A++ + H +T G
Sbjct: 340 FMIDCPGIVPPNSNDSETEIIIKGALRIEKVSNPEQYIHAILNLCETKHLERTYQISGWE 399
Query: 219 KSVLVGLDRLGCIPKLLVNGSCVEEKNVATFLFNDQLKSLV 259
++ L L+ G V+E ++A + ND ++ +
Sbjct: 400 NDSTKFIELLARKTGKLLKGGEVDESSIAKMVINDFIRGKI 440
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.137 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,107,616
Number of Sequences: 164201
Number of extensions: 1799586
Number of successful extensions: 3775
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 3759
Number of HSP's gapped (non-prelim): 12
length of query: 361
length of database: 59,974,054
effective HSP length: 111
effective length of query: 250
effective length of database: 41,747,743
effective search space: 10436935750
effective search space used: 10436935750
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0476a.9