BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0476a.5
(268 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
POFA_SCHPO (Q9P7W4) F-box/WD-repeat protein pof10 (Skp1-binding ... 35 0.25
FXLL_MOUSE (Q8BFZ4) F-box/LRR-repeat protein 21 (F-box and leuci... 34 0.43
FXLC_HUMAN (Q9NXK8) F-box/LRR-repeat protein 12 (F-box and leuci... 32 1.2
CDC4_CANAL (P53699) Cell division control protein 4 32 1.6
SYT_HALN1 (Q9HP27) Threonyl-tRNA synthetase (EC 6.1.1.3) (Threon... 32 2.1
RCA_CUCSA (Q01587) Ribulose bisphosphate carboxylase/oxygenase a... 32 2.1
FXLC_MOUSE (Q9EPX5) F-box/LRR-repeat protein 12 (F-box and leuci... 31 2.8
PSAA_AMPOP (Q9MTQ4) Photosystem I P700 chlorophyll A apoprotein ... 31 3.6
GRK7_HUMAN (Q8WTQ7) G protein-coupled receptor kinase 7 (EC 2.7.... 31 3.6
FX34_HUMAN (Q9NWN3) F-box only protein 34 31 3.6
FX17_HUMAN (Q96EF6) F-box only protein 17 (F-box only protein 26) 31 3.6
UFO_ARATH (Q39090) UNUSUAL FLORAL ORGANS protein 30 4.7
SKP2_HUMAN (Q13309) S-phase kinase-associated protein 2 (F-box p... 30 4.7
FX22_HUMAN (Q8NEZ5) F-box only protein 22 (F-box protein FBX22p44) 30 4.7
PSAA_AMPCA (P58309) Photosystem I P700 chlorophyll A apoprotein ... 30 6.2
CYSP_TRYBB (P14658) Cysteine proteinase precursor (EC 3.4.22.-) 30 6.2
PITB_ECOLI (P43676) Probable low-affinity inorganic phosphate tr... 30 8.0
FX11_RAT (Q7TSL3) F-box only protein 11 30 8.0
FX11_HUMAN (Q86XK2) F-box only protein 11 (Vitiligo-associated p... 30 8.0
>POFA_SCHPO (Q9P7W4) F-box/WD-repeat protein pof10 (Skp1-binding
protein 2)
Length = 662
Score = 34.7 bits (78), Expect = 0.25
Identities = 17/36 (47%), Positives = 21/36 (58%)
Query: 4 LTLPHELIFEILLRLPVRSLLRFKCVCKSWFYLISD 39
L LP E++ I L RSLL +C CK W L+SD
Sbjct: 32 LNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSD 67
>FXLL_MOUSE (Q8BFZ4) F-box/LRR-repeat protein 21 (F-box and
leucine-rich repeat protein 21) (F-box/LRR-repeat
protein 3B) (F-box and leucine-rich repeat protein 3B)
Length = 434
Score = 33.9 bits (76), Expect = 0.43
Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Query: 5 TLPHELIFEILLRLPVRSLLRFKCVCKSWFYLISDPQ-FAKSHFDLNAAPT 54
TLPH +I +I LP+ R VC+ W + P + K F+LN + T
Sbjct: 44 TLPHHVILQIFQYLPLIDRARASSVCRRWNEVFHIPDLWRKFEFELNQSAT 94
>FXLC_HUMAN (Q9NXK8) F-box/LRR-repeat protein 12 (F-box and
leucine-rich repeat protein 12) (F-box protein FBL12)
Length = 326
Score = 32.3 bits (72), Expect = 1.2
Identities = 17/44 (38%), Positives = 23/44 (51%), Gaps = 1/44 (2%)
Query: 6 LPHELIFEILLRLPVRSLLRFKCVCKSWFYLISDPQFAKSHFDL 49
LP ++ EI LPVR +R VC W L+ D ++ H DL
Sbjct: 7 LPDSVLLEIFSYLPVRDRIRISRVCHRWKRLVDD-RWLWRHVDL 49
>CDC4_CANAL (P53699) Cell division control protein 4
Length = 684
Score = 32.0 bits (71), Expect = 1.6
Identities = 14/35 (40%), Positives = 22/35 (62%)
Query: 6 LPHELIFEILLRLPVRSLLRFKCVCKSWFYLISDP 40
+P E+ +IL L ++LL VCK WF +I++P
Sbjct: 218 VPFEVTMKILSYLDYKTLLSVAQVCKKWFDIINNP 252
>SYT_HALN1 (Q9HP27) Threonyl-tRNA synthetase (EC 6.1.1.3)
(Threonine--tRNA ligase) (ThrRS)
Length = 650
Score = 31.6 bits (70), Expect = 2.1
Identities = 20/54 (37%), Positives = 23/54 (42%), Gaps = 5/54 (9%)
Query: 139 YDNSTDDYFLVLIV-----LASMNAKIHAYSVKTNSWDYNHVHVLYMHLGCDYR 187
YDN DD FL+ + L MN HA SW Y + V Y G YR
Sbjct: 320 YDNYVDDMFLLDVNDEEYGLKPMNCPGHATIFNEGSWSYRDLPVRYFEDGKVYR 373
>RCA_CUCSA (Q01587) Ribulose bisphosphate carboxylase/oxygenase
activase, chloroplast precursor (RuBisCO activase) (RA)
Length = 413
Score = 31.6 bits (70), Expect = 2.1
Identities = 11/32 (34%), Positives = 20/32 (62%)
Query: 117 TGFHKQILTSCDFIIGSLYGFGYDNSTDDYFL 148
TG H +L+S ++I L + YDN+ D +++
Sbjct: 99 TGTHNAVLSSYEYISAGLRDYSYDNNVDGFYI 130
>FXLC_MOUSE (Q9EPX5) F-box/LRR-repeat protein 12 (F-box and
leucine-rich repeat protein 12) (F-box protein FBL12)
Length = 326
Score = 31.2 bits (69), Expect = 2.8
Identities = 17/44 (38%), Positives = 23/44 (51%), Gaps = 1/44 (2%)
Query: 6 LPHELIFEILLRLPVRSLLRFKCVCKSWFYLISDPQFAKSHFDL 49
LP ++ EI LPVR +R VC W L+ D ++ H DL
Sbjct: 7 LPDLVLLEIFSYLPVRDRIRISRVCHRWKRLVDD-RWLWRHVDL 49
>PSAA_AMPOP (Q9MTQ4) Photosystem I P700 chlorophyll A apoprotein A1
(PsaA) (PSI-A) (Fragment)
Length = 671
Score = 30.8 bits (68), Expect = 3.6
Identities = 22/94 (23%), Positives = 45/94 (47%), Gaps = 5/94 (5%)
Query: 174 HVHVLYMHLGCDYRHGVFLNNSLHWLVISKVTSLQVVIAYDLSETSLSEIPLLPELAEPV 233
H+ V+++ L + HG + +N L WL + ++ +++ L+ I +
Sbjct: 75 HIGVVFIWLSGMHFHGAYFSNYLDWLQDPSIAPTAQQVSNIANQSVLNPIRVTSGFFNLW 134
Query: 234 LKAECMPTFYQVRVLGG----CLSLCYTGSRWDM 263
L AE + + YQ++V+ +LC+ GS + M
Sbjct: 135 L-AEGITSTYQLKVIAAFGLIASALCFLGSYFHM 167
>GRK7_HUMAN (Q8WTQ7) G protein-coupled receptor kinase 7 (EC
2.7.1.-) (G protein-coupled receptor kinase GRK7)
Length = 553
Score = 30.8 bits (68), Expect = 3.6
Identities = 30/112 (26%), Positives = 47/112 (41%), Gaps = 13/112 (11%)
Query: 121 KQIL--TSCDFIIGSLYGFGYDNSTDDYFLVLIVLASMNAKIHAYSVKTNSWDYNHVHVL 178
K+IL S FI+ Y F S LV+ ++ + K H Y+V T D + V
Sbjct: 240 KEILEKVSSPFIVSLAYAF---ESKTHLCLVMSLMNGGDLKFHIYNVGTRGLDMSRVIFY 296
Query: 179 YMHLGCDYRHGVFLNNSLHWL-VISKVTSLQVVIAYDLSETSLSEIPLLPEL 229
+ C H LH L ++ + + V+ DL LS++ L E+
Sbjct: 297 SAQIACGMLH-------LHELGIVYRDMKPENVLLDDLGNCRLSDLGLAVEM 341
>FX34_HUMAN (Q9NWN3) F-box only protein 34
Length = 305
Score = 30.8 bits (68), Expect = 3.6
Identities = 12/32 (37%), Positives = 20/32 (62%)
Query: 6 LPHELIFEILLRLPVRSLLRFKCVCKSWFYLI 37
LPH ++ +I LP +SL+ KC C + ++I
Sbjct: 172 LPHHIMVKIFRLLPTKSLVALKCTCCYFKFII 203
>FX17_HUMAN (Q96EF6) F-box only protein 17 (F-box only protein 26)
Length = 278
Score = 30.8 bits (68), Expect = 3.6
Identities = 14/36 (38%), Positives = 24/36 (65%), Gaps = 1/36 (2%)
Query: 6 LPHELIFEILLRLPVRSLL-RFKCVCKSWFYLISDP 40
LP EL+ ++L +P RSL+ R + VC++W ++ P
Sbjct: 21 LPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGP 56
>UFO_ARATH (Q39090) UNUSUAL FLORAL ORGANS protein
Length = 442
Score = 30.4 bits (67), Expect = 4.7
Identities = 13/44 (29%), Positives = 24/44 (54%)
Query: 6 LPHELIFEILLRLPVRSLLRFKCVCKSWFYLISDPQFAKSHFDL 49
LP L+ ++ LP + R +CVCK ++ L+ F +++ L
Sbjct: 50 LPPPLLDRVIAFLPPPAFFRTRCVCKRFYSLLFSNTFLETYLQL 93
>SKP2_HUMAN (Q13309) S-phase kinase-associated protein 2 (F-box
protein Skp2) (Cyclin A/CDK2-associated protein p45)
(p45skp2) (F-box/LRR-repeat protein 1)
Length = 424
Score = 30.4 bits (67), Expect = 4.7
Identities = 15/35 (42%), Positives = 20/35 (56%)
Query: 5 TLPHELIFEILLRLPVRSLLRFKCVCKSWFYLISD 39
+LP EL+ I L + LL+ VCK W+ L SD
Sbjct: 99 SLPDELLLGIFSCLCLPELLKVSGVCKRWYRLASD 133
>FX22_HUMAN (Q8NEZ5) F-box only protein 22 (F-box protein
FBX22p44)
Length = 403
Score = 30.4 bits (67), Expect = 4.7
Identities = 11/25 (44%), Positives = 17/25 (68%)
Query: 9 ELIFEILLRLPVRSLLRFKCVCKSW 33
E++ +L LP ++LLR CVC+ W
Sbjct: 28 EVVERVLTFLPAKALLRVACVCRLW 52
>PSAA_AMPCA (P58309) Photosystem I P700 chlorophyll A apoprotein A1
(PsaA) (PSI-A)
Length = 678
Score = 30.0 bits (66), Expect = 6.2
Identities = 22/94 (23%), Positives = 44/94 (46%), Gaps = 5/94 (5%)
Query: 174 HVHVLYMHLGCDYRHGVFLNNSLHWLVISKVTSLQVVIAYDLSETSLSEIPLLPELAEPV 233
H+ V ++ L + HG + +N L WL + ++ +++ L+ I +
Sbjct: 82 HIGVAFIWLSGMHFHGAYFSNYLDWLQDPSIAPTAQQVSNIANQSVLNPIRVTSGFFNLW 141
Query: 234 LKAECMPTFYQVRVLGG----CLSLCYTGSRWDM 263
L AE + + YQ++V+ +LC+ GS + M
Sbjct: 142 L-AEGITSTYQLKVIAAFGLIASALCFLGSYFHM 174
>CYSP_TRYBB (P14658) Cysteine proteinase precursor (EC 3.4.22.-)
Length = 450
Score = 30.0 bits (66), Expect = 6.2
Identities = 18/49 (36%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Query: 95 GSCRGFMLLAN-EYQRVIVWNPSTGFHKQILTSCDFIIGSLYGFGYDNS 142
GSC F + N E Q + NP +Q+L SCD I G DN+
Sbjct: 148 GSCWAFSTIGNIEGQWQVAGNPLVSLSEQMLVSCDTIDSGCNGGLMDNA 196
>PITB_ECOLI (P43676) Probable low-affinity inorganic phosphate
transporter 2
Length = 499
Score = 29.6 bits (65), Expect = 8.0
Identities = 14/49 (28%), Positives = 24/49 (48%)
Query: 94 LGSCRGFMLLANEYQRVIVWNPSTGFHKQILTSCDFIIGSLYGFGYDNS 142
+GS G ++ + I+WN T F +S +IG++ G G N+
Sbjct: 88 MGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNA 136
>FX11_RAT (Q7TSL3) F-box only protein 11
Length = 843
Score = 29.6 bits (65), Expect = 8.0
Identities = 14/35 (40%), Positives = 20/35 (57%)
Query: 6 LPHELIFEILLRLPVRSLLRFKCVCKSWFYLISDP 40
LP E++ +I L + L R CVCK + L +DP
Sbjct: 75 LPDEVVLKIFSYLLEQDLCRAACVCKRFSELANDP 109
>FX11_HUMAN (Q86XK2) F-box only protein 11 (Vitiligo-associated
protein VIT-1)
Length = 843
Score = 29.6 bits (65), Expect = 8.0
Identities = 14/35 (40%), Positives = 20/35 (57%)
Query: 6 LPHELIFEILLRLPVRSLLRFKCVCKSWFYLISDP 40
LP E++ +I L + L R CVCK + L +DP
Sbjct: 75 LPDEVVLKIFSYLLEQDLCRAACVCKRFSELANDP 109
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.327 0.142 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,765,526
Number of Sequences: 164201
Number of extensions: 1323309
Number of successful extensions: 3346
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 3333
Number of HSP's gapped (non-prelim): 20
length of query: 268
length of database: 59,974,054
effective HSP length: 108
effective length of query: 160
effective length of database: 42,240,346
effective search space: 6758455360
effective search space used: 6758455360
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0476a.5