BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0403.9
(139 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PSBR_SOLTU (P06183) Photosystem II 10 kDa polypeptide, chloropla... 227 5e-60
PSBR_LYCES (Q40163) Photosystem II 10 kDa polypeptide, chloropla... 227 5e-60
PSBR_TOBAC (Q40519) Photosystem II 10 kDa polypeptide, chloropla... 219 1e-57
PSBR_SPIOL (P10690) Photosystem II 10 kDa polypeptide, chloropla... 219 1e-57
PSBR_ARATH (P27202) Photosystem II 10 kDa polypeptide, chloropla... 209 2e-54
PSBR_BRACM (P49108) Photosystem II 10 kDa polypeptide, chloropla... 207 7e-54
PSBR_HORVU (Q40070) Photosystem II 10 kDa polypeptide, chloropla... 162 2e-40
PSBR_WHEAT (P12358) Photosystem II 10 kDa polypeptide (Fragment) 32 0.32
YJI2_YEAST (P47031) Hypothetical 82.5 kDa protein in EXO70-ARP4 ... 30 1.2
UBP7_YEAST (P40453) Ubiquitin carboxyl-terminal hydrolase 7 (EC ... 30 1.2
RBS_HORVU (Q40004) Ribulose bisphosphate carboxylase small chain... 30 1.6
R161_YEAST (P25343) Reduced viability upon starvation protein 161 30 2.1
BGL1_SACFI (P22506) Beta-glucosidase 1 precursor (EC 3.2.1.21) (... 30 2.1
ROA1_HUMAN (P09651) Heterogeneous nuclear ribonucleoprotein A1 (... 29 2.7
FHAB_BORPE (P12255) Filamentous hemagglutinin 29 2.7
ALB2_PEA (P08688) Albumin 2 (PA2) 29 2.7
REP1_MOUSE (O54916) RalBP1 associated Eps domain containing prot... 29 3.6
MYSB_ACACA (P19706) Myosin heavy chain IB (Myosin heavy chain IL) 29 3.6
TRMD_CORGL (Q8NNX7) tRNA (Guanine-N(1)-)-methyltransferase (EC 2... 28 4.6
TRMD_COREF (Q8CY26) tRNA (Guanine-N(1)-)-methyltransferase (EC 2... 28 4.6
>PSBR_SOLTU (P06183) Photosystem II 10 kDa polypeptide, chloroplast
precursor (Light inducible tissue-specific ST-LS1
protein)
Length = 138
Score = 227 bits (579), Expect = 5e-60
Identities = 111/139 (79%), Positives = 129/139 (91%), Gaps = 1/139 (0%)
Query: 1 MASSVMASVSLKPAGFSLEKSSVRGLPSLSRSSASFRVVASGGKKIKTDTPYGTGGGMNL 60
MAS+VM+S+SLKP F+LEK+SV+GLPSL+RSS+SF+VVASG KK+KTD PYG G M L
Sbjct: 1 MASTVMSSLSLKPT-FTLEKTSVKGLPSLARSSSSFKVVASGVKKLKTDKPYGINGSMAL 59
Query: 61 RNGVDASGRKPRGKGVYQYVDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAVTL 120
R+GVDASGRKP+GKGVYQYVDKYGANVDGYSPIY+T +WSP+GDVY GGTTGLAIWAVTL
Sbjct: 60 RDGVDASGRKPKGKGVYQYVDKYGANVDGYSPIYNTDEWSPSGDVYVGGTTGLAIWAVTL 119
Query: 121 LGLLAGGALLVYNTSALSQ 139
+G+LAGGALLVYNTSAL+Q
Sbjct: 120 VGILAGGALLVYNTSALAQ 138
>PSBR_LYCES (Q40163) Photosystem II 10 kDa polypeptide, chloroplast
precursor
Length = 138
Score = 227 bits (579), Expect = 5e-60
Identities = 112/139 (80%), Positives = 128/139 (91%), Gaps = 1/139 (0%)
Query: 1 MASSVMASVSLKPAGFSLEKSSVRGLPSLSRSSASFRVVASGGKKIKTDTPYGTGGGMNL 60
MAS+VM+S+SLKP F+LEK SV+GLPSL+RSS+SF+VVASG KK+KTD PYG G M L
Sbjct: 1 MASTVMSSLSLKPT-FTLEKISVKGLPSLTRSSSSFKVVASGVKKLKTDKPYGINGSMAL 59
Query: 61 RNGVDASGRKPRGKGVYQYVDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAVTL 120
R+GVDASGRKP+GKGVYQYVDKYGANVDGYSPIY+T +WSP+GDVY GGTTGLAIWAVTL
Sbjct: 60 RDGVDASGRKPKGKGVYQYVDKYGANVDGYSPIYNTDEWSPSGDVYVGGTTGLAIWAVTL 119
Query: 121 LGLLAGGALLVYNTSALSQ 139
LG+LAGGALLVYNTSAL+Q
Sbjct: 120 LGILAGGALLVYNTSALAQ 138
>PSBR_TOBAC (Q40519) Photosystem II 10 kDa polypeptide, chloroplast
precursor (PII10)
Length = 136
Score = 219 bits (558), Expect = 1e-57
Identities = 108/139 (77%), Positives = 126/139 (89%), Gaps = 3/139 (2%)
Query: 1 MASSVMASVSLKPAGFSLEKSSVRGLPSLSRSSASFRVVASGGKKIKTDTPYGTGGGMNL 60
MAS+VM SLKPA FS+EK++V+GLPSL+RSS+SFRV ASG KK+KTD PYG G M+L
Sbjct: 1 MASTVM---SLKPAPFSVEKTAVKGLPSLARSSSSFRVQASGVKKLKTDKPYGINGSMSL 57
Query: 61 RNGVDASGRKPRGKGVYQYVDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAVTL 120
R+GVDASGRK +GKGVYQ+VDKYGANVDGYSPIY+T DWSP+GDVY GGTTGLAIWAVTL
Sbjct: 58 RDGVDASGRKQKGKGVYQFVDKYGANVDGYSPIYNTDDWSPSGDVYVGGTTGLAIWAVTL 117
Query: 121 LGLLAGGALLVYNTSALSQ 139
+G+LAGGALLV+NTSAL+Q
Sbjct: 118 VGILAGGALLVFNTSALAQ 136
>PSBR_SPIOL (P10690) Photosystem II 10 kDa polypeptide, chloroplast
precursor
Length = 140
Score = 219 bits (558), Expect = 1e-57
Identities = 109/140 (77%), Positives = 125/140 (88%), Gaps = 1/140 (0%)
Query: 1 MASSVMASVSLKPAGFSLE-KSSVRGLPSLSRSSASFRVVASGGKKIKTDTPYGTGGGMN 59
MA+SVM+S+SLKP+ F ++ KS+V+GLPSLSRSSASF V ASG KKIK D P G GGGM
Sbjct: 1 MATSVMSSLSLKPSSFGVDTKSAVKGLPSLSRSSASFTVRASGVKKIKVDKPLGIGGGMK 60
Query: 60 LRNGVDASGRKPRGKGVYQYVDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAVT 119
LR+GVD+SGRKP GKGVYQ+VDKYGANVDGYSPIY+ ++W+PTGDVYAGGTTGL IWAVT
Sbjct: 61 LRDGVDSSGRKPTGKGVYQFVDKYGANVDGYSPIYNEEEWAPTGDVYAGGTTGLLIWAVT 120
Query: 120 LLGLLAGGALLVYNTSALSQ 139
L GLLAGGALLVYNTSAL+Q
Sbjct: 121 LAGLLAGGALLVYNTSALAQ 140
>PSBR_ARATH (P27202) Photosystem II 10 kDa polypeptide, chloroplast
precursor
Length = 140
Score = 209 bits (531), Expect = 2e-54
Identities = 102/140 (72%), Positives = 123/140 (87%), Gaps = 1/140 (0%)
Query: 1 MASSVM-ASVSLKPAGFSLEKSSVRGLPSLSRSSASFRVVASGGKKIKTDTPYGTGGGMN 59
MA+SVM +SV+LKPAGF++EK++ RGLPSL+R+ SF++VASG KKIKTD P+G G M+
Sbjct: 1 MAASVMLSSVTLKPAGFTVEKTAARGLPSLTRARPSFKIVASGVKKIKTDKPFGINGSMD 60
Query: 60 LRNGVDASGRKPRGKGVYQYVDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAVT 119
LR+GVDASGRK +G GVY+YVDKYGANVDGYSPIY+ +WS +GDVY GG TGLAIWAVT
Sbjct: 61 LRDGVDASGRKGKGYGVYKYVDKYGANVDGYSPIYNENEWSASGDVYKGGVTGLAIWAVT 120
Query: 120 LLGLLAGGALLVYNTSALSQ 139
L G+LAGGALLVYNTSAL+Q
Sbjct: 121 LAGILAGGALLVYNTSALAQ 140
>PSBR_BRACM (P49108) Photosystem II 10 kDa polypeptide, chloroplast
precursor
Length = 141
Score = 207 bits (526), Expect = 7e-54
Identities = 104/141 (73%), Positives = 124/141 (87%), Gaps = 2/141 (1%)
Query: 1 MASSVM-ASVSLKPAGFSLEKSSVRGLPSLSRSS-ASFRVVASGGKKIKTDTPYGTGGGM 58
MA+SVM +SV+LKPAGF++EK S RGLPSL+R+S +SFR+VASG KKIKTD P+G G M
Sbjct: 1 MAASVMLSSVTLKPAGFTVEKMSARGLPSLTRASPSSFRIVASGVKKIKTDKPFGVNGSM 60
Query: 59 NLRNGVDASGRKPRGKGVYQYVDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAV 118
+LR+GVDASGRK +G GVY++VDKYGANVDGYSPIY+ +WS +GDVY GG TGLAIWAV
Sbjct: 61 DLRDGVDASGRKGKGYGVYKFVDKYGANVDGYSPIYNEDEWSASGDVYKGGVTGLAIWAV 120
Query: 119 TLLGLLAGGALLVYNTSALSQ 139
TL G+LAGGALLVYNTSAL+Q
Sbjct: 121 TLAGILAGGALLVYNTSALAQ 141
>PSBR_HORVU (Q40070) Photosystem II 10 kDa polypeptide, chloroplast
precursor
Length = 138
Score = 162 bits (410), Expect = 2e-40
Identities = 81/139 (58%), Positives = 105/139 (75%), Gaps = 3/139 (2%)
Query: 1 MASSVMASVSLKPAGFSL-EKSSVRGLPSLSRSSASFRVVASGGKKIKTDTPYGTGGGMN 59
M++ VMAS++LKP+ L ++S + G+ +R S +VA KK++T PYG GGG+
Sbjct: 1 MSACVMASLALKPSSSPLLQRSKLGGVRPSARPSLV--IVAKKAKKVQTAQPYGPGGGVA 58
Query: 60 LRNGVDASGRKPRGKGVYQYVDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAVT 119
+ GVDASGR +GKGVYQ+ DKYGANVDGYSPIY ++WSP+GDVY GG TGL +WAVT
Sbjct: 59 FKEGVDASGRVAKGKGVYQFADKYGANVDGYSPIYTPEEWSPSGDVYVGGKTGLFLWAVT 118
Query: 120 LLGLLAGGALLVYNTSALS 138
L G+L GGALLVY+TSAL+
Sbjct: 119 LAGILLGGALLVYSTSALA 137
>PSBR_WHEAT (P12358) Photosystem II 10 kDa polypeptide (Fragment)
Length = 24
Score = 32.3 bits (72), Expect = 0.32
Identities = 13/20 (65%), Positives = 15/20 (75%)
Query: 41 SGGKKIKTDTPYGTGGGMNL 60
SGGKKIK D P G GGG+ +
Sbjct: 1 SGGKKIKVDKPLGLGGGLTV 20
>YJI2_YEAST (P47031) Hypothetical 82.5 kDa protein in EXO70-ARP4
intergenic region
Length = 731
Score = 30.4 bits (67), Expect = 1.2
Identities = 18/55 (32%), Positives = 21/55 (37%)
Query: 82 KYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAVTLLGLLAGGALLVYNTSA 136
+Y D + D DWS Y G L W +T LGLL G Y A
Sbjct: 470 EYNRAADDLISLLDISDWSHALYTYFAGCCYLENWRMTQLGLLNDGKEQFYKERA 524
>UBP7_YEAST (P40453) Ubiquitin carboxyl-terminal hydrolase 7 (EC
3.1.2.15) (Ubiquitin thiolesterase 7)
(Ubiquitin-specific processing protease 7)
(Deubiquitinating enzyme 7)
Length = 1071
Score = 30.4 bits (67), Expect = 1.2
Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Query: 31 RSSASFRVVASGGKKIKTDTPYGTGGGMNLRNGVDASGRK 70
RS + FR++ GK+ + TP+ TGG + N D +K
Sbjct: 907 RSRSPFRMLGGSGKRSSSSTPFSTGGN-DSNNSSDYKNKK 945
>RBS_HORVU (Q40004) Ribulose bisphosphate carboxylase small chain,
chloroplast precursor (EC 4.1.1.39) (RuBisCO small
subunit)
Length = 174
Score = 30.0 bits (66), Expect = 1.6
Identities = 20/44 (45%), Positives = 27/44 (60%), Gaps = 1/44 (2%)
Query: 1 MASSVMASVSLKPAGFSLEKSSVRGLPSLSRSSASFRVVASGGK 44
MA +VMAS + A F KS+ GLP RS+AS V++GG+
Sbjct: 1 MAPTVMASSATSVAPFQGLKSTA-GLPVSRRSNASSASVSNGGR 43
>R161_YEAST (P25343) Reduced viability upon starvation protein 161
Length = 265
Score = 29.6 bits (65), Expect = 2.1
Identities = 18/52 (34%), Positives = 27/52 (51%)
Query: 11 LKPAGFSLEKSSVRGLPSLSRSSASFRVVASGGKKIKTDTPYGTGGGMNLRN 62
L+ AG +L+K + L SL +AS +A + D+ Y GGG N+ N
Sbjct: 40 LQRAGEALQKEAKGFLDSLRAVTASQTTIAEVISNLYDDSKYVAGGGYNVGN 91
>BGL1_SACFI (P22506) Beta-glucosidase 1 precursor (EC 3.2.1.21)
(Gentiobiase) (Cellobiase) (Beta-D-glucoside
glucohydrolase)
Length = 876
Score = 29.6 bits (65), Expect = 2.1
Identities = 14/41 (34%), Positives = 18/41 (43%)
Query: 80 VDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTGLAIWAVTL 120
V +GA + G D S G+VY G TG + W L
Sbjct: 293 VSDWGAQLSGVYSAISGLDMSMPGEVYGGWNTGTSFWGQNL 333
>ROA1_HUMAN (P09651) Heterogeneous nuclear ribonucleoprotein A1
(Helix-destabilizing protein) (Single-strand binding
protein) (hnRNP core protein A1)
Length = 371
Score = 29.3 bits (64), Expect = 2.7
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Query: 53 GTGGGMNLRNGVDASGRKPRGKGVYQYVDKYGANVDGYSPIYDTKDWSPTGDVYAGGTTG 112
G GG N G + SGR G G + YG + DGY+ + + G Y+GG+ G
Sbjct: 208 GFGGNDNFGRGGNFSGRG--GFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRG 265
>FHAB_BORPE (P12255) Filamentous hemagglutinin
Length = 3590
Score = 29.3 bits (64), Expect = 2.7
Identities = 25/63 (39%), Positives = 30/63 (46%), Gaps = 12/63 (19%)
Query: 21 SSVRGLPSLSRSSASFRVVASGGKKIKTDTPYGTGGGMNLRNGVDASGR-------KPRG 73
SSV GL S +V A GK + D+ G GGG+ + VD SGR K RG
Sbjct: 2519 SSVNGLIQ----GRSVKVDAGKGKVVVADSK-GAGGGIEADDEVDVSGRDIGIEGGKLRG 2573
Query: 74 KGV 76
K V
Sbjct: 2574 KDV 2576
>ALB2_PEA (P08688) Albumin 2 (PA2)
Length = 231
Score = 29.3 bits (64), Expect = 2.7
Identities = 18/47 (38%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Query: 42 GGKKIKTDTPYGTGGGMNLRNGVDASGRKPRGKGVYQYVDKYGANVD 88
G KKI P+ G NG+DA+ R RGK VY + A +D
Sbjct: 101 GPKKIADMFPFFEG--TVFENGIDAAYRSTRGKEVYLFKGDQYARID 145
>REP1_MOUSE (O54916) RalBP1 associated Eps domain containing protein
1 (RalBP1-interacting protein 1)
Length = 743
Score = 28.9 bits (63), Expect = 3.6
Identities = 10/19 (52%), Positives = 13/19 (67%)
Query: 99 WSPTGDVYAGGTTGLAIWA 117
WSP GD AG + G A+W+
Sbjct: 149 WSPFGDAQAGSSAGDAVWS 167
>MYSB_ACACA (P19706) Myosin heavy chain IB (Myosin heavy chain IL)
Length = 1147
Score = 28.9 bits (63), Expect = 3.6
Identities = 19/63 (30%), Positives = 27/63 (42%), Gaps = 6/63 (9%)
Query: 19 EKSSVRGLPSLSRSSASFRVVASGGKKIKTD------TPYGTGGGMNLRNGVDASGRKPR 72
+ S PS+ +S A+ ++ + G +TD TP G GGG G GR
Sbjct: 877 KNESASAQPSIKKSRANIQIGIATGLPKETDSSPPNWTPSGGGGGYGGGRGGGGGGRGAA 936
Query: 73 GKG 75
G G
Sbjct: 937 GGG 939
>TRMD_CORGL (Q8NNX7) tRNA (Guanine-N(1)-)-methyltransferase (EC
2.1.1.31) (M1G-methyltransferase) (tRNA [GM37]
methyltransferase)
Length = 289
Score = 28.5 bits (62), Expect = 4.6
Identities = 12/22 (54%), Positives = 15/22 (67%)
Query: 40 ASGGKKIKTDTPYGTGGGMNLR 61
A+GG K DTPYG G GM ++
Sbjct: 52 ATGGHKAVDDTPYGGGPGMVMK 73
>TRMD_COREF (Q8CY26) tRNA (Guanine-N(1)-)-methyltransferase (EC
2.1.1.31) (M1G-methyltransferase) (tRNA [GM37]
methyltransferase)
Length = 291
Score = 28.5 bits (62), Expect = 4.6
Identities = 12/22 (54%), Positives = 15/22 (67%)
Query: 40 ASGGKKIKTDTPYGTGGGMNLR 61
A+GG K DTPYG G GM ++
Sbjct: 56 ATGGHKAVDDTPYGGGPGMVMK 77
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.312 0.132 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,845,552
Number of Sequences: 164201
Number of extensions: 851329
Number of successful extensions: 1886
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 1859
Number of HSP's gapped (non-prelim): 33
length of query: 139
length of database: 59,974,054
effective HSP length: 99
effective length of query: 40
effective length of database: 43,718,155
effective search space: 1748726200
effective search space used: 1748726200
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0403.9