BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0387.5
(124 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LEPA_CHLTR (O84067) GTP-binding protein lepA 35 0.030
LEPA_CHLMU (Q9PKX6) GTP-binding protein lepA 35 0.039
TBP_DROME (P20227) TATA-box binding protein (TATA-box factor) (T... 33 0.11
LEPA_CHLCV (Q823H7) GTP-binding protein lepA 33 0.11
LEPA_CHLPN (Q9Z8I4) GTP-binding protein lepA 32 0.19
CUP9_YEAST (P41817) Homeobox protein CUP9 31 0.43
MAZ4_SCHCO (P37938) Mating-type protein A-alpha Z4 30 0.95
KLF1_HUMAN (Q13351) Erythroid krueppel-like transcription factor... 30 0.95
SD23_SCHPO (Q09826) Protein sds23/moc1 (Phosphoprotein at statio... 29 1.6
ISTA_SHISO (P16944) Transposase for insertion sequence element I... 28 2.8
ISTA_PSEAE (P15025) Transposase for insertion sequence element IS21 28 2.8
TAT_SIVS4 (P12513) TAT protein (Transactivating regulatory protein) 28 3.6
RNFC_HAEIN (P71397) Electron transport complex protein rnfC 28 3.6
YAB4_SCHPO (Q09806) Hypothetical protein C2G11.04 in chromosome I 28 4.7
Y893_HUMAN (O94967) Hypothetical protein KIAA0893 28 4.7
V1A_CCMV (P27752) 1A protein [Includes: Helicase; Methyltransfer... 28 4.7
HDC_DROME (Q9N2M8) Headcase protein [Contains: Headcase short pr... 28 4.7
BAB2_DROME (Q9W0K4) Bric-a-brac protein 2 28 4.7
PESC_HUMAN (O00541) Pescadillo homolog 1 27 6.2
NEBU_HUMAN (P20929) Nebulin 27 6.2
>LEPA_CHLTR (O84067) GTP-binding protein lepA
Length = 602
Score = 35.0 bits (79), Expect = 0.030
Identities = 24/96 (25%), Positives = 42/96 (43%), Gaps = 1/96 (1%)
Query: 19 QLSLLIMDEDDDPLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQSTQPR 78
+L LI D DP V+ + SG I + + + + G G F+P++T
Sbjct: 194 ELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMATKGSSFEVLGIGAFLPEATLME 253
Query: 79 RKQRKGRTSSYAKYQKQSQDTRMISHV-PIKNPFKE 113
R G+ + K+ +D ++ V +K+P KE
Sbjct: 254 GSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKE 289
>LEPA_CHLMU (Q9PKX6) GTP-binding protein lepA
Length = 602
Score = 34.7 bits (78), Expect = 0.039
Identities = 24/96 (25%), Positives = 42/96 (43%), Gaps = 1/96 (1%)
Query: 19 QLSLLIMDEDDDPLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQSTQPR 78
+L LI D DP V+ + SG I + + + + G G F+P++T
Sbjct: 194 ELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMSTKGSSFEVLGIGAFLPEATLIE 253
Query: 79 RKQRKGRTSSYAKYQKQSQDTRMISHV-PIKNPFKE 113
R G+ + K+ +D ++ V +K+P KE
Sbjct: 254 GSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKE 289
>TBP_DROME (P20227) TATA-box binding protein (TATA-box factor) (TATA
binding factor) (TATA sequence-binding protein)
(Transcription initiation factor TFIID TBP subunit)
Length = 353
Score = 33.1 bits (74), Expect = 0.11
Identities = 23/87 (26%), Positives = 42/87 (47%), Gaps = 5/87 (5%)
Query: 25 MDEDDDPLASCAVHPQSFS--GAIHPPPQSAILYEQVLRRQSK---GTGVFIPQSTQPRR 79
M+ D +A+ HP + S ++ P P S+ + Q ++QS G+G+F + + P
Sbjct: 21 MEADQQIVANPVYHPPAVSQPDSLMPAPGSSSVQHQQQQQQSDASGGSGLFGHEPSLPLA 80
Query: 80 KQRKGRTSSYAKYQKQSQDTRMISHVP 106
++ A YQ+Q Q ++ S P
Sbjct: 81 HKQMQSYQPSASYQQQQQQQQLQSQAP 107
>LEPA_CHLCV (Q823H7) GTP-binding protein lepA
Length = 602
Score = 33.1 bits (74), Expect = 0.11
Identities = 23/95 (24%), Positives = 41/95 (42%), Gaps = 1/95 (1%)
Query: 19 QLSLLIMDEDDDPLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQSTQPR 78
+L LI D DP V+ + SG I + + + + G G F+P++T
Sbjct: 194 ELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMATKGSAFEVLGVGAFLPEATLIE 253
Query: 79 RKQRKGRTSSYAKYQKQSQDTRMISHV-PIKNPFK 112
R G+ + K+ +D ++ V +K+P K
Sbjct: 254 GSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAK 288
>LEPA_CHLPN (Q9Z8I4) GTP-binding protein lepA
Length = 602
Score = 32.3 bits (72), Expect = 0.19
Identities = 26/110 (23%), Positives = 44/110 (39%), Gaps = 8/110 (7%)
Query: 14 ADLSKQLSLLIMDEDDDPLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQ 73
A +L L+ D DP V+ + SG + + + + + G G F+P+
Sbjct: 189 APAETELKALVFDSHYDPYVGIMVYVRIISGELKKGDRITFMAAKGSSFEVLGIGAFLPK 248
Query: 74 STQPRRKQRKGRTSSYAKYQKQSQDTRMISHV-----PIKNP---FKETN 115
+T R G+ + K+ +D ++ V P K P FKE N
Sbjct: 249 ATFIEGSLRPGQVGFFIANLKKVKDVKIGDTVTKTKHPAKTPLEGFKEIN 298
>CUP9_YEAST (P41817) Homeobox protein CUP9
Length = 306
Score = 31.2 bits (69), Expect = 0.43
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 6/82 (7%)
Query: 40 QSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQS----TQPRRKQRKGRTSSYAKYQKQ 95
+S S P +SA +YE + + + G P S ++ RRKQ GR S+ K Q
Sbjct: 116 KSASDDAKPCYKSAPIYEIINKEKDAGAQYNRPFSDFVESKSRRKQNSGRRSNLPKETVQ 175
Query: 96 SQDTRMISHVPIKNPFKETNDR 117
+T +++H + NP+ ++
Sbjct: 176 ILNTWLLNH--LNNPYPTQQEK 195
>MAZ4_SCHCO (P37938) Mating-type protein A-alpha Z4
Length = 940
Score = 30.0 bits (66), Expect = 0.95
Identities = 18/71 (25%), Positives = 33/71 (46%)
Query: 28 DDDPLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTS 87
DD+ ++ A P+ FS + P PQ+ + S+G+ P S P+ K+ TS
Sbjct: 482 DDEEVSPLAKKPRIFSPPVRPRPQAIRVSLPSPAPSSRGSTPTSPVSPSPKAKRPAQATS 541
Query: 88 SYAKYQKQSQD 98
A + + ++
Sbjct: 542 LLASHPMKKRE 552
>KLF1_HUMAN (Q13351) Erythroid krueppel-like transcription factor
(EKLF) (Erythroid transcription factor)
Length = 362
Score = 30.0 bits (66), Expect = 0.95
Identities = 19/53 (35%), Positives = 26/53 (48%), Gaps = 4/53 (7%)
Query: 6 ELEDDLFFADLSKQLSLLIMD----EDDDPLASCAVHPQSFSGAIHPPPQSAI 54
E EDD AD + L LL+ + E +CA+ P SGA +PPP +
Sbjct: 61 EEEDDERGADATWDLDLLLTNFSGPEPGGAPQTCALAPSEASGAQYPPPPETL 113
>SD23_SCHPO (Q09826) Protein sds23/moc1 (Phosphoprotein at
stationary phase 1 protein)
Length = 408
Score = 29.3 bits (64), Expect = 1.6
Identities = 17/42 (40%), Positives = 21/42 (49%)
Query: 59 VLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTR 100
VL KGT P S R R+G TSS+ + K S DT+
Sbjct: 365 VLYAHMKGTLPPAPHSAPSLRHGRRGSTSSHRSHSKASVDTQ 406
>ISTA_SHISO (P16944) Transposase for insertion sequence element
IS640
Length = 315
Score = 28.5 bits (62), Expect = 2.8
Identities = 10/27 (37%), Positives = 18/27 (66%)
Query: 70 FIPQSTQPRRKQRKGRTSSYAKYQKQS 96
F+P++ +PRR + KG+ KY K++
Sbjct: 213 FLPRACRPRRARTKGKVERMVKYLKEN 239
>ISTA_PSEAE (P15025) Transposase for insertion sequence element IS21
Length = 390
Score = 28.5 bits (62), Expect = 2.8
Identities = 10/27 (37%), Positives = 18/27 (66%)
Query: 70 FIPQSTQPRRKQRKGRTSSYAKYQKQS 96
F+P++ +PRR + KG+ KY K++
Sbjct: 213 FLPRACRPRRARTKGKVERMVKYLKEN 239
>TAT_SIVS4 (P12513) TAT protein (Transactivating regulatory protein)
Length = 117
Score = 28.1 bits (61), Expect = 3.6
Identities = 28/103 (27%), Positives = 42/103 (40%), Gaps = 16/103 (15%)
Query: 25 MDEDDDPLASCAVHPQSFSGAIHPPPQSAIL---YEQVLRRQ----------SKGTGVFI 71
+ E ++ L SC H S S P P+SA L Y + ++ KG G+
Sbjct: 5 LKEQENSLESCREHSSSISEVDVPTPESANLEACYNKCYCKRCCYHCQHCFLKKGLGICY 64
Query: 72 PQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKET 114
Q RR +K + + + +S TR + P K KET
Sbjct: 65 EQ--HRRRTPKKTKANPFPASNNRSLSTRARNRQP-KKEKKET 104
>RNFC_HAEIN (P71397) Electron transport complex protein rnfC
Length = 819
Score = 28.1 bits (61), Expect = 3.6
Identities = 13/31 (41%), Positives = 15/31 (47%)
Query: 42 FSGAIHPPPQSAILYEQVLRRQSKGTGVFIP 72
F G IHPP + Q LR GT +IP
Sbjct: 16 FKGGIHPPEMKSQSNSQPLRHLPLGTDFYIP 46
>YAB4_SCHPO (Q09806) Hypothetical protein C2G11.04 in chromosome I
Length = 301
Score = 27.7 bits (60), Expect = 4.7
Identities = 13/45 (28%), Positives = 21/45 (45%)
Query: 70 FIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKET 114
F+P + +P+ ++K SSY Q I HV +N + T
Sbjct: 63 FLPSNFRPKTHKKKKSVSSYLSSTIQKNANENIPHVQCRNDSQST 107
>Y893_HUMAN (O94967) Hypothetical protein KIAA0893
Length = 919
Score = 27.7 bits (60), Expect = 4.7
Identities = 11/29 (37%), Positives = 17/29 (57%)
Query: 15 DLSKQLSLLIMDEDDDPLASCAVHPQSFS 43
DL+KQL ++++ E D + C H Q S
Sbjct: 874 DLTKQLPIMVVGEHKDKVIQCRWHTQDLS 902
>V1A_CCMV (P27752) 1A protein [Includes: Helicase;
Methyltransferase]
Length = 958
Score = 27.7 bits (60), Expect = 4.7
Identities = 14/38 (36%), Positives = 21/38 (54%)
Query: 80 KQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKETNDR 117
K+ RTS Y ++ S+ +R ISH I +P + T R
Sbjct: 825 KRGGNRTSKYLGWKSHSKVSRSISHKEIASPLQVTLSR 862
>HDC_DROME (Q9N2M8) Headcase protein [Contains: Headcase short
protein]
Length = 1080
Score = 27.7 bits (60), Expect = 4.7
Identities = 16/69 (23%), Positives = 34/69 (49%), Gaps = 8/69 (11%)
Query: 47 HPPPQSAILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHVP 106
HP Q ++Q +R+Q + Q QP+++Q++ + + + +Q Q + H P
Sbjct: 719 HPHAQQ---HQQQVRQQQQQQ-----QQQQPQQQQQQQQQQTQQQQSQQQQQQQQQQHQP 770
Query: 107 IKNPFKETN 115
+ + F+ N
Sbjct: 771 VVSTFQRGN 779
>BAB2_DROME (Q9W0K4) Bric-a-brac protein 2
Length = 1067
Score = 27.7 bits (60), Expect = 4.7
Identities = 27/103 (26%), Positives = 41/103 (39%), Gaps = 9/103 (8%)
Query: 15 DLSKQLSLLIMDEDDDPLASC-AVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQ 73
+L+ ++L D D DP A P+ P+ + Q R++S+ P
Sbjct: 334 ELASATAILQQDGDADPDEEMKAKRPRLL-------PEGVLDLNQRQRKRSRDGSYATPS 386
Query: 74 -STQPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKETN 115
S Q + R SS Q QSQ M + ++NPF N
Sbjct: 387 PSLQGGESEISERGSSGTPGQSQSQPLAMTTSTIVRNPFASPN 429
>PESC_HUMAN (O00541) Pescadillo homolog 1
Length = 588
Score = 27.3 bits (59), Expect = 6.2
Identities = 12/38 (31%), Positives = 22/38 (57%)
Query: 76 QPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKE 113
+P+ K++ + S+ A+ +D R + H PI N F+E
Sbjct: 47 EPKHKKKVNKGSTAARTFYLIKDIRFLLHEPIVNKFRE 84
>NEBU_HUMAN (P20929) Nebulin
Length = 6669
Score = 27.3 bits (59), Expect = 6.2
Identities = 14/62 (22%), Positives = 28/62 (44%)
Query: 52 SAILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPF 111
S++LY R+Q +G ++ + + RR + R S KY + + + + +P
Sbjct: 6398 SSVLYSDSFRKQIQGKAAYVLDTPEMRRVRETQRHISTVKYHEDFEKHKGCFTPVVTDPI 6457
Query: 112 KE 113
E
Sbjct: 6458 TE 6459
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.131 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,735,038
Number of Sequences: 164201
Number of extensions: 565514
Number of successful extensions: 1411
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1397
Number of HSP's gapped (non-prelim): 26
length of query: 124
length of database: 59,974,054
effective HSP length: 100
effective length of query: 24
effective length of database: 43,553,954
effective search space: 1045294896
effective search space used: 1045294896
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0387.5