BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0371b.6
(264 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RPA1_DROME (P91875) DNA-directed RNA polymerase I largest subuni... 33 0.54
TOP1_ARATH (P30181) DNA topoisomerase I (EC 5.99.1.2) 32 1.2
SCD1_SCHPO (P40995) Protein scd1 32 1.2
G6PI_BIFLO (Q8G7I6) Glucose-6-phosphate isomerase (EC 5.3.1.9) (... 32 1.6
CSE1_YEAST (P33307) Importin alpha re-exporter (Chromosome segre... 32 1.6
MLL3_MOUSE (Q8BRH4) Myeloid/lymphoid or mixed-lineage leukemia p... 32 2.1
YS89_CAEEL (Q09624) Hypothetical protein ZK945.9 in chromosome II 31 3.5
ST20_CANAL (Q92212) Serine/threonine-protein kinase STE20 homolo... 31 3.5
CHD1_MOUSE (P40201) Chromodomain-helicase-DNA-binding protein 1 ... 31 3.5
NAGB_STRP8 (Q8P0E0) Glucosamine-6-phosphate deaminase (EC 3.5.99... 30 4.6
SPEN_DROME (Q8SX83) Split ends protein 30 6.0
NAGB_BACSU (O35000) Glucosamine-6-phosphate deaminase 1 (EC 3.5.... 30 6.0
IMB3_MOUSE (Q8BKC5) Importin beta-3 (Karyopherin beta-3) (Ran-bi... 30 7.8
IMB3_HUMAN (O00410) Importin beta-3 (Karyopherin beta-3) (Ran-bi... 30 7.8
IBP5_PIG (Q28985) Insulin-like growth factor binding protein 5 p... 30 7.8
CAIC_SALTY (Q8ZRX4) Probable crotonobetaine/carnitine-CoA ligase... 30 7.8
CAIC_SALTI (Q8Z9L4) Probable crotonobetaine/carnitine-CoA ligase... 30 7.8
BPA1_MOUSE (Q91ZU6) Bullous pemphigoid antigen 1, isoforms 1/2/3... 30 7.8
>RPA1_DROME (P91875) DNA-directed RNA polymerase I largest subunit (EC
2.7.7.6)
Length = 1644
Score = 33.5 bits (75), Expect = 0.54
Identities = 24/72 (33%), Positives = 31/72 (42%), Gaps = 5/72 (6%)
Query: 12 DDGSLDGGDENQIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPIQFVE---- 67
DD LD GDE K+N + SD D + T K + T + +DDP E
Sbjct: 1344 DDNDLDNGDEVGRSKAKANDDDSSD-DNDDDDATGVKLKQRKTDEKDYDDPDDVEELHDA 1402
Query: 68 NTEDDHVGDNSD 79
N +DD D D
Sbjct: 1403 NDDDDEAEDEDD 1414
>TOP1_ARATH (P30181) DNA topoisomerase I (EC 5.99.1.2)
Length = 916
Score = 32.3 bits (72), Expect = 1.2
Identities = 28/117 (23%), Positives = 49/117 (40%), Gaps = 20/117 (17%)
Query: 77 NSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMKMDSPKSSPR-----------V 125
NS +++ K KA V + ++ + + ++K++ SP S V
Sbjct: 320 NSTSSAAKPKARNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGV 379
Query: 126 LFPPPLDAGALKFEDKGEAMEVMVSPRRKVENEMATMDCDREEVD----PTDGFNFW 178
+FPPP +K KG+ +++ + + E+ATM E D P NFW
Sbjct: 380 IFPPPYKPHGIKILYKGKPVDLTIE-----QEEVATMFAVMRETDYYTKPQFRENFW 431
>SCD1_SCHPO (P40995) Protein scd1
Length = 872
Score = 32.3 bits (72), Expect = 1.2
Identities = 37/140 (26%), Positives = 59/140 (41%), Gaps = 16/140 (11%)
Query: 29 SNPESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPIQFVENTEDDHVGDNSDTTSEKIKAV 88
S+P SKS + F T+ K S+TT + P F+ ++ VG +S TS+ +
Sbjct: 604 SSPSSKS----SPFTKTTSKDTKSATTT--DERPSDFIRLNSEESVGTSSLRTSQ-TTST 656
Query: 89 VVEADEQETLSV-SQF--FLQLENKFADMKMDSPKSSPRVLFPPPLDAGALKFEDKGEAM 145
+V D T S+ SQ Q+ + D + RV + FED
Sbjct: 657 IVSNDSSSTASIPSQISRISQVNSLLNDYNYNRQSHITRVYSGTDDGSSVSIFEDTSS-- 714
Query: 146 EVMVSPRRKVENEMATMDCD 165
S ++K+ ++ T DCD
Sbjct: 715 ----STKQKIFDQPTTNDCD 730
>G6PI_BIFLO (Q8G7I6) Glucose-6-phosphate isomerase (EC 5.3.1.9)
(GPI) (Phosphoglucose isomerase) (PGI) (Phosphohexose
isomerase) (PHI)
Length = 566
Score = 32.0 bits (71), Expect = 1.6
Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 5/50 (10%)
Query: 169 VDPTDGFNFWKWSLTGVGAICSFGVAAATICVLVFGSQQKNKF-HQNHKI 217
+DP + F FW W VG S A T +VFG + +F H H+I
Sbjct: 274 IDPNNAFGFWNW----VGGRYSVDSAVGTSLAVVFGPARFEEFLHGFHEI 319
>CSE1_YEAST (P33307) Importin alpha re-exporter (Chromosome
segregation protein CSE1)
Length = 960
Score = 32.0 bits (71), Expect = 1.6
Identities = 18/67 (26%), Positives = 29/67 (42%), Gaps = 3/67 (4%)
Query: 56 QLLHDDPIQFVENTEDDHVGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMK 115
+L DDPI+++ D G ++DT + E E+ + V+ FL F D
Sbjct: 355 ELFEDDPIEYIRR---DLEGSDTDTRRRACTDFLKELKEKNEVLVTNIFLAHMKGFVDQY 411
Query: 116 MDSPKSS 122
M P +
Sbjct: 412 MSDPSKN 418
>MLL3_MOUSE (Q8BRH4) Myeloid/lymphoid or mixed-lineage leukemia
protein 3 homolog (Histone-lysine N-methyltransferase, H3
lysine-4 specific MLL3) (EC 2.1.1.43)
Length = 4903
Score = 31.6 bits (70), Expect = 2.1
Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 10/69 (14%)
Query: 118 SPKSSPRVLFPPPLDAGALKFEDKGEAMEVMVSPRRKVENEMATMDCDREEVDPTD---- 173
SP +SP + FPP +A K E K + ++V V K++ + T+ E+ P +
Sbjct: 4292 SPPASPPISFPPAFEAA--KVESKPDELKVTV----KLKPRLRTVPVGLEDCRPLNKKWR 4345
Query: 174 GFNFWKWSL 182
G + KWS+
Sbjct: 4346 GMKWKKWSI 4354
>YS89_CAEEL (Q09624) Hypothetical protein ZK945.9 in chromosome II
Length = 3178
Score = 30.8 bits (68), Expect = 3.5
Identities = 27/107 (25%), Positives = 45/107 (41%), Gaps = 8/107 (7%)
Query: 45 TSPKSRDSSTTQL-----LHDDPIQFVENTEDDHVGDNSDTTSEKIKAVVVEADEQETLS 99
+S +S DS+TT L + E+T D SDT++ AD TLS
Sbjct: 693 SSTRSSDSTTTSAGSTTTLQESTTTSEESTTDSSTTTISDTSTTSSSPSSTTADSTSTLS 752
Query: 100 VSQFFLQLENKFADMKMDSPKSSPRVLFPPPLDAGALKFEDKGEAME 146
V QF L++ + + + S ++ PL A+ ++ + E
Sbjct: 753 VDQFDFILDSGLSWNETRHNEDSINIV---PLPTNAITPTERSQTFE 796
>ST20_CANAL (Q92212) Serine/threonine-protein kinase STE20 homolog
(EC 2.7.1.37)
Length = 1230
Score = 30.8 bits (68), Expect = 3.5
Identities = 34/147 (23%), Positives = 61/147 (41%), Gaps = 29/147 (19%)
Query: 4 TNDWVLLSDDGSLDGGDE-------NQIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQ 56
TND + S G +D + N +SNPES S+ D P+ + +
Sbjct: 89 TNDRTIESGSGDIDDSQQSHNNNNNNNNNNNESNPES-SEGDDEKTQGMPPRMPGTFNVK 147
Query: 57 LLH-----DDPIQFVENTEDDHVGDNSDTTS---------------EKIKAVVVEADEQE 96
LH D+ Q+ E T+ + + D+ S + V+E +EQ
Sbjct: 148 GLHQGDDSDNEKQYTELTKSINKRTSKDSYSPGTLESPGTLNALETNNVSPAVIE-EEQH 206
Query: 97 TLSVSQFFLQLENKFADMKMDSPKSSP 123
T S+ L L+++ + ++ +P+S+P
Sbjct: 207 TSSLEDLSLSLQHQNENARLSAPRSAP 233
>CHD1_MOUSE (P40201) Chromodomain-helicase-DNA-binding protein 1
(CHD-1)
Length = 1711
Score = 30.8 bits (68), Expect = 3.5
Identities = 15/61 (24%), Positives = 29/61 (46%)
Query: 31 PESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPIQFVENTEDDHVGDNSDTTSEKIKAVVV 90
P KSD D + + P+S+D S ++ D P+ + E + + S+ +K ++
Sbjct: 1359 PSEKSDEDDDKLNDSKPESKDRSKKSVVSDAPVHITASGEPVPIAEESEELDQKTFSICK 1418
Query: 91 E 91
E
Sbjct: 1419 E 1419
>NAGB_STRP8 (Q8P0E0) Glucosamine-6-phosphate deaminase (EC 3.5.99.6)
(Glucosamine-6-phosphate isomerase) (GNPDA) (GlcN6P
deaminase)
Length = 234
Score = 30.4 bits (67), Expect = 4.6
Identities = 23/95 (24%), Positives = 43/95 (45%), Gaps = 5/95 (5%)
Query: 20 DENQIFLGKSNPESKSDFDMNYF---YCTSPKSRDSSTTQLLHDDPIQF--VENTEDDHV 74
D++ + + N + F NY T+ ++ Q++ + PI F + + H+
Sbjct: 72 DQSYDYFMRQNLFNAKPFKKNYLPNGLATNIEAEAKRYDQIIAEHPIDFQVLGIGRNGHI 131
Query: 75 GDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLEN 109
G N TS K + VV+ E + S+FF +E+
Sbjct: 132 GFNEPGTSFKEETHVVDLQESTIEANSRFFTSIED 166
>SPEN_DROME (Q8SX83) Split ends protein
Length = 5560
Score = 30.0 bits (66), Expect = 6.0
Identities = 28/114 (24%), Positives = 49/114 (42%), Gaps = 8/114 (7%)
Query: 21 ENQIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPIQFVENTEDDHVGDNSDT 80
E+ + + P+S+ D ++ +P DSS L D+ +Q + E + +NS T
Sbjct: 3399 EDMMDIKADTPQSERDLQIDTDTEENPDEADSSGPSLKIDETVQSSSSPEKS-ISNNSPT 3457
Query: 81 TSEKIKAVVVEADEQETL---SVSQFFLQLENKFADMKMDSPKSSPRVLFPPPL 131
E + + Q L S Q + + F +D+PK+ P L P P+
Sbjct: 3458 PRETANIDIPNVESQPKLSNESTPQPSVITKLPF----LDTPKTVPAGLPPSPV 3507
>NAGB_BACSU (O35000) Glucosamine-6-phosphate deaminase 1 (EC
3.5.99.6) (Glucosamine-6-phosphate isomerase 1) (GNPDA
1) (GlcN6P deaminase 1)
Length = 242
Score = 30.0 bits (66), Expect = 6.0
Identities = 27/96 (28%), Positives = 46/96 (47%), Gaps = 6/96 (6%)
Query: 58 LHDDPIQFVENTEDDHVGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLEN---KFADM 114
L D IQ + + H+G N TS K + VV +EQ + +++F +++ K M
Sbjct: 123 LGDTDIQLLGIGRNGHIGFNEPGTSFKSRTHVVTLNEQTRQANARYFPSIDSVPKKALTM 182
Query: 115 KMDSPKSSPRVLFPPPLDAGALKFEDKGEAMEVMVS 150
+ + SS R+L L +G K E + +E +S
Sbjct: 183 GIQTILSSKRILL---LISGKSKAEAVRKLLEGNIS 215
>IMB3_MOUSE (Q8BKC5) Importin beta-3 (Karyopherin beta-3)
(Ran-binding protein 5) (RanBP5)
Length = 1097
Score = 29.6 bits (65), Expect = 7.8
Identities = 32/118 (27%), Positives = 50/118 (42%), Gaps = 19/118 (16%)
Query: 87 AVVVEADEQETLSVSQFFLQLENKFADMKMDSPKSSPRVLFPPPLDAGALKFEDKGEAME 146
AV E Q+ V Q L+ + F DM+ D P+ S + A A + G+ +
Sbjct: 567 AVGKEKFMQDASDVMQLLLKTQTDFNDMEDDDPQISYMI------SAWARMCKILGKEFQ 620
Query: 147 ----VMVSPRRK---VENEMATMDC-DREEVDPTDGFNFWKWSLTGVGAICSFGVAAA 196
V++ P K ++ E+A +D D E + DG W +G SFG+ A
Sbjct: 621 QYLPVVMGPLMKTASIKPEVALLDTQDMENMSDDDG-----WEFVNLGDQQSFGIKTA 673
>IMB3_HUMAN (O00410) Importin beta-3 (Karyopherin beta-3)
(Ran-binding protein 5) (RanBP5)
Length = 1097
Score = 29.6 bits (65), Expect = 7.8
Identities = 32/118 (27%), Positives = 50/118 (42%), Gaps = 19/118 (16%)
Query: 87 AVVVEADEQETLSVSQFFLQLENKFADMKMDSPKSSPRVLFPPPLDAGALKFEDKGEAME 146
AV E Q+ V Q L+ + F DM+ D P+ S + A A + G+ +
Sbjct: 567 AVGKEKFMQDASDVMQLLLKTQTDFNDMEDDDPQISYMI------SAWARMCKILGKEFQ 620
Query: 147 ----VMVSPRRK---VENEMATMDC-DREEVDPTDGFNFWKWSLTGVGAICSFGVAAA 196
V++ P K ++ E+A +D D E + DG W +G SFG+ A
Sbjct: 621 QYLPVVMGPLMKTASIKPEVALLDTQDMENMSDDDG-----WEFVNLGDQQSFGIKTA 673
>IBP5_PIG (Q28985) Insulin-like growth factor binding protein 5
precursor (IGFBP-5) (IBP-5) (IGF-binding protein 5)
Length = 271
Score = 29.6 bits (65), Expect = 7.8
Identities = 27/115 (23%), Positives = 48/115 (41%), Gaps = 17/115 (14%)
Query: 59 HDDPI--QFVENTEDDHVGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMKM 116
H++P + E T + T ++KA V+ D ++ L+ S+F EN
Sbjct: 118 HEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAEN------- 170
Query: 117 DSPKSSPRVLFPPPL----DAGALKFEDKGEAMEVMVSPRRKVENEMATMDCDRE 167
+ PRV+ P + + G + + E+ SP R V + +CDR+
Sbjct: 171 ---TAHPRVILAPEMRQESEQGPCRRHMEASLQELKASP-RMVPRAVYLPNCDRK 221
>CAIC_SALTY (Q8ZRX4) Probable crotonobetaine/carnitine-CoA ligase
(EC 6.3.2.-)
Length = 517
Score = 29.6 bits (65), Expect = 7.8
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Query: 74 VGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMKMDS 118
VG E IKA +V +E ETLS ++FF EN A K+ S
Sbjct: 452 VGIKDAIRDEAIKAFIV-LNEGETLSEAEFFSFCENNMAKFKVPS 495
>CAIC_SALTI (Q8Z9L4) Probable crotonobetaine/carnitine-CoA ligase
(EC 6.3.2.-)
Length = 517
Score = 29.6 bits (65), Expect = 7.8
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Query: 74 VGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMKMDS 118
VG E IKA +V +E ETLS ++FF EN A K+ S
Sbjct: 452 VGIKDAIRDEAIKAFIV-LNEGETLSEAEFFSFCENNMAKFKVPS 495
>BPA1_MOUSE (Q91ZU6) Bullous pemphigoid antigen 1, isoforms 1/2/3/4
(BPA) (Hemidesmosomal plaque protein) (Dystonia
musculorum protein) (Dystonin)
Length = 7389
Score = 29.6 bits (65), Expect = 7.8
Identities = 15/57 (26%), Positives = 26/57 (45%)
Query: 2 DTTNDWVLLSDDGSLDGGDENQIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQLL 58
D T+D LS G++D GD L ++ +D D + ++ +P D L+
Sbjct: 2332 DNTSDCGPLSHKGAIDAGDYETSLLAGQQSDTATDSDSDDYFYDTPLFEDEDHDSLI 2388
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.132 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,833,850
Number of Sequences: 164201
Number of extensions: 1294369
Number of successful extensions: 3499
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 3495
Number of HSP's gapped (non-prelim): 21
length of query: 264
length of database: 59,974,054
effective HSP length: 108
effective length of query: 156
effective length of database: 42,240,346
effective search space: 6589493976
effective search space used: 6589493976
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0371b.6