BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0360.9
(107 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MYSN_DROME (Q99323) Myosin heavy chain, non-muscle (Zipper prote... 33 0.092
MYH9_RAT (Q62812) Myosin heavy chain, nonmuscle type A (Cellular... 33 0.16
MYH9_CHICK (P14105) Myosin heavy chain, nonmuscle (Cellular myos... 32 0.20
MYHB_RABIT (P35748) Myosin heavy chain, smooth muscle isoform (S... 32 0.27
MYH9_HUMAN (P35579) Myosin heavy chain, nonmuscle type A (Cellul... 32 0.35
M18A_MOUSE (Q9JMH9) Myosin XVIIIA (Myosin 18A) (Myosin containin... 31 0.45
GYRA_TREPA (O83051) DNA gyrase subunit A (EC 5.99.1.3) 31 0.45
SYS_STRP8 (Q8NZN7) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--... 31 0.59
MYSB_CAEEL (P02566) Myosin heavy chain B (MHC B) 30 0.78
MYHA_BOVIN (Q27991) Myosin heavy chain, nonmuscle type B (Cellul... 30 0.78
GOA4_MOUSE (Q91VW5) Golgi autoantigen, golgin subfamily A member... 30 0.78
REST_CHICK (O42184) Restin (Cytoplasmic linker protein-170) (CLI... 30 1.0
MYHB_CHICK (P10587) Myosin heavy chain, gizzard smooth muscle 30 1.0
M18A_HUMAN (Q92614) Myosin XVIIIA (Myosin 18A) (Myosin containin... 30 1.0
YC86_STAAM (P67277) Hypothetical UPF0144 protein SAV1286 30 1.3
YB69_STAAW (P67279) Hypothetical UPF0144 protein MW1169 30 1.3
YB29_STAAN (P67278) Hypothetical UPF0144 protein SA1129 30 1.3
MERA_STAEP (P08663) Mercuric reductase (EC 1.16.1.1) (Hg(II) red... 30 1.3
YJ00_AQUAE (O67738) Hypothetical protein AQ_1900 29 1.7
MYHA_RAT (Q9JLT0) Myosin heavy chain, nonmuscle type B (Cellular... 29 1.7
>MYSN_DROME (Q99323) Myosin heavy chain, non-muscle (Zipper protein)
(Myosin II) (Non-muscle MHC)
Length = 2057
Score = 33.5 bits (75), Expect = 0.092
Identities = 27/80 (33%), Positives = 44/80 (54%), Gaps = 8/80 (10%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA*EA 60
+++EL+ L E+E+ R+E Q+ T L Q+ E++T L L+ SA K A + E+
Sbjct: 1344 QIAELQVKL-AEIERARSELQEKCTKLQQEAENITNQLEEAELK-ASAAVKSASNM--ES 1399
Query: 61 KIEEID*KEQQVLPEEDNSK 80
++ E QQ+L EE K
Sbjct: 1400 QLTE----AQQLLEEETRQK 1415
>MYH9_RAT (Q62812) Myosin heavy chain, nonmuscle type A (Cellular
myosin heavy chain, type A) (Nonmuscle myosin heavy
chain-A) (NMMHC-A)
Length = 1961
Score = 32.7 bits (73), Expect = 0.16
Identities = 26/72 (36%), Positives = 38/72 (52%), Gaps = 7/72 (9%)
Query: 6 KKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA--*EAKIE 63
K+ L E+E L+TE +D + QQE LR+ Q+VS K E +A EA+I+
Sbjct: 1134 KRDLGEELEALKTELEDTLDSTAAQQE-----LRSKREQEVSILKKTLEDEAKTHEAQIQ 1188
Query: 64 EID*KEQQVLPE 75
E+ K Q + E
Sbjct: 1189 EMRQKHSQAVEE 1200
>MYH9_CHICK (P14105) Myosin heavy chain, nonmuscle (Cellular myosin
heavy chain) (NMMHC)
Length = 1959
Score = 32.3 bits (72), Expect = 0.20
Identities = 25/72 (34%), Positives = 38/72 (52%), Gaps = 7/72 (9%)
Query: 6 KKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA--*EAKIE 63
K+ L E+E L+TE +D + QQE LR+ Q+V+ K E +A EA+I+
Sbjct: 1134 KRDLGEELEALKTELEDTLDSTAAQQE-----LRSKREQEVTVLKKTLEDEAKTHEAQIQ 1188
Query: 64 EID*KEQQVLPE 75
E+ K Q + E
Sbjct: 1189 EMRQKHSQAIEE 1200
>MYHB_RABIT (P35748) Myosin heavy chain, smooth muscle isoform (SMMHC)
Length = 1972
Score = 32.0 bits (71), Expect = 0.27
Identities = 25/72 (34%), Positives = 39/72 (53%), Gaps = 7/72 (9%)
Query: 6 KKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGK--KAESQA*EAKIE 63
K+ L E+E L+TE +D T QQE LR Q+V+ K E+++ EA+++
Sbjct: 1141 KRDLGEELEALKTELEDTLDTTATQQE-----LRAKREQEVTVLKKALDEETRSHEAQVQ 1195
Query: 64 EID*KEQQVLPE 75
E+ K QV+ E
Sbjct: 1196 EMRQKHTQVVEE 1207
>MYH9_HUMAN (P35579) Myosin heavy chain, nonmuscle type A (Cellular
myosin heavy chain, type A) (Nonmuscle myosin heavy
chain-A) (NMMHC-A)
Length = 1960
Score = 31.6 bits (70), Expect = 0.35
Identities = 25/72 (34%), Positives = 38/72 (52%), Gaps = 7/72 (9%)
Query: 6 KKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA--*EAKIE 63
K+ L E+E L+TE +D + QQE LR+ Q+V+ K E +A EA+I+
Sbjct: 1134 KRDLGEELEALKTELEDTLDSTAAQQE-----LRSKREQEVNILKKTLEEEAKTHEAQIQ 1188
Query: 64 EID*KEQQVLPE 75
E+ K Q + E
Sbjct: 1189 EMRQKHSQAVEE 1200
>M18A_MOUSE (Q9JMH9) Myosin XVIIIA (Myosin 18A) (Myosin containing PDZ
domain)
Length = 2035
Score = 31.2 bits (69), Expect = 0.45
Identities = 19/76 (25%), Positives = 40/76 (52%), Gaps = 1/76 (1%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA*EA 60
++++ K L ++ +L+ E +++ L + QED+ ++ A A+ +A
Sbjct: 1718 DIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDMAQMNDLQA 1777
Query: 61 KIEEID*KEQQVLPEE 76
+IEE + KE+Q L E+
Sbjct: 1778 QIEESN-KEKQELQEK 1792
>GYRA_TREPA (O83051) DNA gyrase subunit A (EC 5.99.1.3)
Length = 813
Score = 31.2 bits (69), Expect = 0.45
Identities = 21/81 (25%), Positives = 42/81 (50%), Gaps = 11/81 (13%)
Query: 4 ELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLG--------LQDVSADGKKAES 55
++K+ +EVE LRTE +DL + +E +T+ + LG + D+ D ++ +
Sbjct: 432 QMKRLTGLEVESLRTELKDLTELISSLEELLTSPQKVLGVVKKETRDIADMFGDDRRTDI 491
Query: 56 QA*EAK---IEEID*KEQQVL 73
+ E + +E+ KE+ V+
Sbjct: 492 VSNEIEYLDVEDFIQKEEMVI 512
>SYS_STRP8 (Q8NZN7) Seryl-tRNA synthetase (EC 6.1.1.11)
(Serine--tRNA ligase) (SerRS)
Length = 425
Score = 30.8 bits (68), Expect = 0.59
Identities = 21/59 (35%), Positives = 33/59 (55%), Gaps = 5/59 (8%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQ---QQEDVTASLRNLGLQDVSADGKKAESQ 56
EL E ++TL V+ E+L+ E + Q Q+ED T + + +Q VSAD K ++Q
Sbjct: 33 ELDEKRRTLLVQSEELKAERNIASAAIAQAKRQKEDATQQIAD--MQKVSADIKTIDNQ 89
>MYSB_CAEEL (P02566) Myosin heavy chain B (MHC B)
Length = 1966
Score = 30.4 bits (67), Expect = 0.78
Identities = 24/97 (24%), Positives = 45/97 (45%), Gaps = 3/97 (3%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTA---SLRNLGLQDVSADGKKAESQA 57
++ KK + EVE L+ + QDL +L + + + + +R+L + D A+
Sbjct: 947 DVQRAKKKIEAEVEALKKQIQDLEMSLRKAESEKQSKDHQIRSLQDEMQQQDEAIAKLNK 1006
Query: 58 *EAKIEEID*KEQQVLPEEDNSKDAEN*TGFSLEYLL 94
+ EEI+ K + L E++ + +N LE L
Sbjct: 1007 EKKHQEEINRKLMEDLQSEEDKGNHQNKVKAKLEQTL 1043
Score = 29.6 bits (65), Expect = 1.3
Identities = 24/88 (27%), Positives = 43/88 (48%), Gaps = 4/88 (4%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLR---TTLHQQQEDVTASLRNLGLQDVSADGKKAESQA 57
+L E K +L +E +T+ D L QQ+D + L L Q + + A+ Q
Sbjct: 891 KLVEEKTSLFTNLESTKTQLSDAEERLAKLEAQQKDASKQLSELNDQLADNEDRTADVQR 950
Query: 58 *EAKIE-EID*KEQQVLPEEDNSKDAEN 84
+ KIE E++ ++Q+ E + + AE+
Sbjct: 951 AKKKIEAEVEALKKQIQDLEMSLRKAES 978
>MYHA_BOVIN (Q27991) Myosin heavy chain, nonmuscle type B (Cellular
myosin heavy chain, type B) (Nonmuscle myosin heavy
chain-B) (NMMHC-B)
Length = 1976
Score = 30.4 bits (67), Expect = 0.78
Identities = 26/73 (35%), Positives = 41/73 (55%), Gaps = 9/73 (12%)
Query: 6 KKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKA---ESQA*EAKI 62
K+ L+ E+E L+TE +D T QQE LR Q+V A+ KKA E+++ EA+I
Sbjct: 1141 KRDLSEELEALKTELEDTLDTTAAQQE-----LRTKREQEV-AELKKALEEETKSHEAQI 1194
Query: 63 EEID*KEQQVLPE 75
+++ + L E
Sbjct: 1195 QDMRQRHATALEE 1207
Score = 27.3 bits (59), Expect = 6.6
Identities = 21/83 (25%), Positives = 43/83 (51%), Gaps = 7/83 (8%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA*EA 60
EL + K+ L +VE++RT+ ++L L Q ED L +V+ KA+ +
Sbjct: 1529 ELEKSKRALEQQVEEMRTQLEELEDEL-QATEDAKLRL------EVNMQAMKAQFERDLQ 1581
Query: 61 KIEEID*KEQQVLPEEDNSKDAE 83
+E + +++++L ++ +AE
Sbjct: 1582 TRDEQNEEKKRLLIKQVRELEAE 1604
>GOA4_MOUSE (Q91VW5) Golgi autoantigen, golgin subfamily A member 4
(tGolgin-1)
Length = 2238
Score = 30.4 bits (67), Expect = 0.78
Identities = 23/85 (27%), Positives = 43/85 (50%), Gaps = 10/85 (11%)
Query: 5 LKKTLNVEVEQLRT---EFQDLRTTLHQQQEDVTASLRN----LGLQDVSADGKKAESQA 57
+ K VE E L + E Q L + ++++D+ + R L ++ GK + Q
Sbjct: 2031 IDKAQEVEAELLESHQEETQQLHRKIAEKEDDLRRTARRYEEILDAREEEMTGKVTDLQT 2090
Query: 58 *EAKIEEID*KEQQVLPEEDNSKDA 82
++EE+ K QQ L +E+++KD+
Sbjct: 2091 ---QLEELQKKYQQRLEQEESTKDS 2112
>REST_CHICK (O42184) Restin (Cytoplasmic linker protein-170)
(CLIP-170)
Length = 1433
Score = 30.0 bits (66), Expect = 1.0
Identities = 21/73 (28%), Positives = 37/73 (49%), Gaps = 1/73 (1%)
Query: 2 LSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGL-QDVSADGKKAESQA*EA 60
+ ELK + N V EF +L+T + + + D + NL L Q+ E +A +A
Sbjct: 628 MEELKVSFNKGVGAQTAEFAELKTQMEKVKLDYENEMSNLKLKQENEKSQHLKEIEALKA 687
Query: 61 KIEEID*KEQQVL 73
K+ E+ +++Q L
Sbjct: 688 KLLEVTEEKEQTL 700
Score = 28.1 bits (61), Expect = 3.9
Identities = 19/67 (28%), Positives = 37/67 (54%), Gaps = 4/67 (5%)
Query: 1 ELSELKKTLNVE---VEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA 57
EL++ K+ LN+E VE+L+ EF+ L+ Q+ + + A+L+ ++ G+ +
Sbjct: 1127 ELTKSKELLNLENKKVEELKKEFEALKLAAAQKSQQL-AALQEENVKLAEELGRSRDEVT 1185
Query: 58 *EAKIEE 64
K+EE
Sbjct: 1186 SHQKLEE 1192
>MYHB_CHICK (P10587) Myosin heavy chain, gizzard smooth muscle
Length = 1978
Score = 30.0 bits (66), Expect = 1.0
Identities = 23/72 (31%), Positives = 38/72 (51%), Gaps = 7/72 (9%)
Query: 6 KKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSA--DGKKAESQA*EAKIE 63
K+ L+ E+E L+TE +D T QQE LR Q+V+ + E++ EA+++
Sbjct: 1146 KRDLSEELEALKTELEDTLDTTATQQE-----LRAKREQEVTVLKRALEEETRTHEAQVQ 1200
Query: 64 EID*KEQQVLPE 75
E+ K Q + E
Sbjct: 1201 EMRQKHTQAVEE 1212
Score = 28.5 bits (62), Expect = 2.9
Identities = 21/76 (27%), Positives = 42/76 (54%), Gaps = 8/76 (10%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAE-SQA*E 59
EL + K+TL +VE+++T+ ++L L Q ED L +V+ K++ + +
Sbjct: 1534 ELEKSKRTLEQQVEEMKTQLEELEDEL-QAAEDAKLRL------EVNMQAMKSQFERDLQ 1586
Query: 60 AKIEEID*KEQQVLPE 75
A+ E+ + K +Q+L +
Sbjct: 1587 ARDEQNEEKRRQLLKQ 1602
>M18A_HUMAN (Q92614) Myosin XVIIIA (Myosin 18A) (Myosin containing PDZ
domain)
Length = 2054
Score = 30.0 bits (66), Expect = 1.0
Identities = 18/76 (23%), Positives = 40/76 (51%), Gaps = 1/76 (1%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA*EA 60
++++ K L ++ +L+ E +++ L + QED+ ++ A A+ +A
Sbjct: 1722 DIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQA 1781
Query: 61 KIEEID*KEQQVLPEE 76
++EE + KE+Q L E+
Sbjct: 1782 QLEEAN-KEKQELQEK 1796
>YC86_STAAM (P67277) Hypothetical UPF0144 protein SAV1286
Length = 519
Score = 29.6 bits (65), Expect = 1.3
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA*EA 60
+L E K+ + EQ E ++ R+ L +Q+ + NL + D K + E+
Sbjct: 57 KLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSDLLDKKDEILEQKES 116
Query: 61 KIEEID*KEQQVLPEEDN 78
KIEE K+QQV +E +
Sbjct: 117 KIEE---KQQQVDAKESS 131
>YB69_STAAW (P67279) Hypothetical UPF0144 protein MW1169
Length = 519
Score = 29.6 bits (65), Expect = 1.3
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA*EA 60
+L E K+ + EQ E ++ R+ L +Q+ + NL + D K + E+
Sbjct: 57 KLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSDLLDKKDEILEQKES 116
Query: 61 KIEEID*KEQQVLPEEDN 78
KIEE K+QQV +E +
Sbjct: 117 KIEE---KQQQVDAKESS 131
>YB29_STAAN (P67278) Hypothetical UPF0144 protein SA1129
Length = 519
Score = 29.6 bits (65), Expect = 1.3
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA*EA 60
+L E K+ + EQ E ++ R+ L +Q+ + NL + D K + E+
Sbjct: 57 KLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSDLLDKKDEILEQKES 116
Query: 61 KIEEID*KEQQVLPEEDN 78
KIEE K+QQV +E +
Sbjct: 117 KIEE---KQQQVDAKESS 131
>MERA_STAEP (P08663) Mercuric reductase (EC 1.16.1.1) (Hg(II)
reductase)
Length = 547
Score = 29.6 bits (65), Expect = 1.3
Identities = 20/64 (31%), Positives = 35/64 (54%), Gaps = 6/64 (9%)
Query: 31 QEDVTASLRNLGLQDVSADGKKAESQA*EAKIEEID*KEQQV-----LPEEDNSKDAEN* 85
+E VT +L G +DVSAD ++ E+ E ++I+ +Q + P E+ S+ +EN
Sbjct: 19 EEHVTEALEQAGAKDVSADFRRGEA-IFELSDDQIEKAKQNISAAGYQPGEEESQPSENS 77
Query: 86 TGFS 89
F+
Sbjct: 78 VDFN 81
>YJ00_AQUAE (O67738) Hypothetical protein AQ_1900
Length = 168
Score = 29.3 bits (64), Expect = 1.7
Identities = 12/34 (35%), Positives = 22/34 (64%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDV 34
+++E+K TL +EQ FQDL +++ +ED+
Sbjct: 30 DIAEIKATLKTFMEQTDKRFQDLNQRINELREDM 63
>MYHA_RAT (Q9JLT0) Myosin heavy chain, nonmuscle type B (Cellular
myosin heavy chain, type B) (Nonmuscle myosin heavy
chain-B) (NMMHC-B)
Length = 1976
Score = 29.3 bits (64), Expect = 1.7
Identities = 26/73 (35%), Positives = 40/73 (54%), Gaps = 9/73 (12%)
Query: 6 KKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKA---ESQA*EAKI 62
K+ L+ E+E L+TE +D T QQE LR Q+V A+ KKA E++ EA+I
Sbjct: 1141 KRDLSEELEALKTELEDTLDTTAAQQE-----LRTKREQEV-AELKKALEDETKNHEAQI 1194
Query: 63 EEID*KEQQVLPE 75
+++ + L E
Sbjct: 1195 QDMRQRHATALEE 1207
Score = 27.3 bits (59), Expect = 6.6
Identities = 21/83 (25%), Positives = 43/83 (51%), Gaps = 7/83 (8%)
Query: 1 ELSELKKTLNVEVEQLRTEFQDLRTTLHQQQEDVTASLRNLGLQDVSADGKKAESQA*EA 60
EL + K+ L +VE++RT+ ++L L Q ED L +V+ KA+ +
Sbjct: 1529 ELEKSKRALEQQVEEMRTQLEELEDEL-QATEDAKLRL------EVNMQAMKAQFERDLQ 1581
Query: 61 KIEEID*KEQQVLPEEDNSKDAE 83
+E + +++++L ++ +AE
Sbjct: 1582 TRDEQNEEKKRLLLKQVRELEAE 1604
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.331 0.144 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,547,751
Number of Sequences: 164201
Number of extensions: 293865
Number of successful extensions: 1623
Number of sequences better than 10.0: 60
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 39
Number of HSP's that attempted gapping in prelim test: 1558
Number of HSP's gapped (non-prelim): 104
length of query: 107
length of database: 59,974,054
effective HSP length: 83
effective length of query: 24
effective length of database: 46,345,371
effective search space: 1112288904
effective search space used: 1112288904
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0360.9