BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0358.5
         (189 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

CA14_ARATH (Q9SHV5) Putative clathrin assembly protein At2g01920       33  0.51
LEU2_BACCR (Q81G10) 3-isopropylmalate dehydratase large subunit ...    31  1.5
NODA_MOUSE (P43021) Nodal precursor                                    30  4.3
LEU2_BACAN (Q81T66) 3-isopropylmalate dehydratase large subunit ...    28  9.7
FAS5_RHOFA (P46377) Hypothetical 47.9 kDa oxidoreductase in fasc...    28  9.7
DCUA_WOLSU (O34245) Anaerobic C4-dicarboxylate transporter dcuA        28  9.7
DCUA_HELPY (O25425) Anaerobic C4-dicarboxylate transporter dcuA        28  9.7
DCUA_HELPJ (Q9ZLC0) Anaerobic C4-dicarboxylate transporter dcuA        28  9.7

>CA14_ARATH (Q9SHV5) Putative clathrin assembly protein At2g01920
          Length = 308

 Score = 32.7 bits (73), Expect = 0.51
 Identities = 25/87 (28%), Positives = 42/87 (47%), Gaps = 23/87 (26%)

Query: 104 VYDTVNARHGAFKPLFKAISNATAELKVALTTRFQYKTWGIKSK----FHGLHLQGILPI 159
           +Y  + +   +FKP+ +A+S     L+V  T     + W +  K     HGL   GI+ +
Sbjct: 48  IYRYIQSNPSSFKPIIRAVS-----LRVEHT-----RNWTVALKCLMLLHGLFFSGIMTV 97

Query: 160 DSDGKL-------SRKKKKYPLSRSSR 179
           DS G+L        R+K ++  SR+ R
Sbjct: 98  DSIGRLPFDLSGFGRRKSRF--SRTGR 122


>LEU2_BACCR (Q81G10) 3-isopropylmalate dehydratase large subunit (EC
           4.2.1.33) (Isopropylmalate isomerase) (Alpha-IPM
           isomerase) (IPMI)
          Length = 464

 Score = 31.2 bits (69), Expect = 1.5
 Identities = 23/60 (38%), Positives = 30/60 (49%), Gaps = 5/60 (8%)

Query: 99  GKTRSVYDTVNARHGAFKPLFKAISNATAELKVALTTRFQYKTWGIKSKFHGLHLQGILP 158
           GKT    D+  A HGAF  L  A    T+E++  L T+     W  K K  G+ L+G LP
Sbjct: 120 GKTIVCGDSHTATHGAFGAL--AFGIGTSEVEHVLATQ---TLWQRKPKAMGIELKGKLP 174


>NODA_MOUSE (P43021) Nodal precursor
          Length = 354

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 27/94 (28%), Positives = 36/94 (37%), Gaps = 18/94 (19%)

Query: 23  LCLWLALRPKSPSYSVVFLSIEQHPGENGSIFYSLEIENPNKDSSIYYDDIILSFLYGQQ 82
           L LW   +P SPS     LS+ + P     I  SL+ ++ +     +      SFL  Q+
Sbjct: 28  LPLWTRGQPSSPSPLAYMLSLYRDPLPRADIIRSLQAQDVDVTGQNWTFTFDFSFL-SQE 86

Query: 83  EDKV-----------------GETTIGSFHQGTG 99
           ED V                 G  TI  FHQ  G
Sbjct: 87  EDLVWADVRLQLPGPMDIPTEGPLTIDIFHQAKG 120


>LEU2_BACAN (Q81T66) 3-isopropylmalate dehydratase large subunit (EC
           4.2.1.33) (Isopropylmalate isomerase) (Alpha-IPM
           isomerase) (IPMI)
          Length = 464

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 22/59 (37%), Positives = 29/59 (48%), Gaps = 5/59 (8%)

Query: 99  GKTRSVYDTVNARHGAFKPLFKAISNATAELKVALTTRFQYKTWGIKSKFHGLHLQGIL 157
           GKT    D+  A HGAF  L  A    T+E++  L T+     W  K K  G+ L+G L
Sbjct: 120 GKTIVCGDSHTATHGAFGAL--AFGIGTSEVEHVLATQ---TLWQRKPKAMGIELKGKL 173


>FAS5_RHOFA (P46377) Hypothetical 47.9 kDa oxidoreductase in
           fasciation locus (ORF5)
          Length = 438

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 25/100 (25%), Positives = 43/100 (43%), Gaps = 13/100 (13%)

Query: 79  YGQQEDKVGETTI----GSFHQGTGKTRS-VYDTVNARHGAFKPLFKAISNATAELKVAL 133
           +G Q D V    +    G F + +  + S ++D V    G F  +  A    TA  +   
Sbjct: 139 FGLQTDNVDSLAVVTGSGDFRECSAVSNSELFDAVRGGLGQFGVIVNATIRLTAAHESVR 198

Query: 134 TTRFQYKTWGI------KSKFHGL--HLQGILPIDSDGKL 165
             + QY   G+      ++  + L  H+QG + +D+DG L
Sbjct: 199 QYKLQYSNLGVFLGDQLRAMSNRLFDHVQGRIRVDADGHL 238


>DCUA_WOLSU (O34245) Anaerobic C4-dicarboxylate transporter dcuA
          Length = 433

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 14/38 (36%), Positives = 21/38 (54%)

Query: 4  GKRFYVFLMEFIGFLGLLVLCLWLALRPKSPSYSVVFL 41
          G R     + + G LG++VLCL L L+P S    V+ +
Sbjct: 16 GVRLGGIAIGYAGGLGMIVLCLGLGLKPGSIPIDVILI 53


>DCUA_HELPY (O25425) Anaerobic C4-dicarboxylate transporter dcuA
          Length = 443

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 11/28 (39%), Positives = 18/28 (64%)

Query: 14 FIGFLGLLVLCLWLALRPKSPSYSVVFL 41
          + G LG+L+LCL+L L P    + V+ +
Sbjct: 28 YAGGLGVLILCLFLGLNPGKIPFDVILI 55


>DCUA_HELPJ (Q9ZLC0) Anaerobic C4-dicarboxylate transporter dcuA
          Length = 443

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 11/28 (39%), Positives = 18/28 (64%)

Query: 14 FIGFLGLLVLCLWLALRPKSPSYSVVFL 41
          + G LG+L+LCL+L L P    + V+ +
Sbjct: 28 YAGGLGVLILCLFLGLNPGKIPFDVILI 55


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.320    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,920,131
Number of Sequences: 164201
Number of extensions: 896617
Number of successful extensions: 2035
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 2031
Number of HSP's gapped (non-prelim): 8
length of query: 189
length of database: 59,974,054
effective HSP length: 104
effective length of query: 85
effective length of database: 42,897,150
effective search space: 3646257750
effective search space used: 3646257750
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)