BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0346.3
(374 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PTC1_CYNPY (O42335) Patched protein homolog 1 (PTC1) (Fragment) 34 0.69
TOP2_PEA (O24308) DNA topoisomerase II (EC 5.99.1.3) (PsTopII) 33 0.90
CN3B_RAT (Q63085) cGMP-inhibited 3',5'-cyclic phosphodiesterase ... 33 1.5
SOMA_XENLA (P12855) Somatotropin A precursor (Growth hormone A) ... 32 2.0
RGSC_RAT (O08774) Regulator of G-protein signaling 12 (RGS12) 32 2.6
GPD2_ARATH (Q9FY99) Glucose-6-phosphate 1-dehydrogenase 2, chlor... 32 2.6
YSNF_BACSU (P94560) Stress response protein ysnF 32 3.4
UCRH_YEAST (P00127) Ubiquinol-cytochrome C reductase complex 17 ... 32 3.4
STRQ_STRPY (P96471) Streptokinase precursor 32 3.4
PTVB_ECOLI (P32154) PTS system, fructose-like-1 IIBC component (... 32 3.4
NUCL_MESAU (P08199) Nucleolin (Protein C23) 32 3.4
NUOG_CANBF (Q7VRV7) NADH-quinone oxidoreductase chain 3 (EC 1.6.... 31 4.5
TUBE_DROVI (Q08171) Tube protein 31 5.8
TOP1_USTMA (P41511) DNA topoisomerase I (EC 5.99.1.2) 31 5.8
TPR_HUMAN (P12270) Nucleoprotein TPR 30 7.6
SIS2_CANTR (Q12600) SIS2 protein (Halotolerance protein HAL3) 30 7.6
RPIA_ARATH (Q9ZU38) Probable-ribose 5-phosphate isomerase (EC 5.... 30 7.6
UBC_ASFM2 (P25869) Ubiquitin-conjugating enzyme E2-21 kDa (EC 6.... 30 9.9
SELA_HAEDU (Q7VKC2) L-seryl-tRNA(Sec) selenium transferase (EC 2... 30 9.9
PGM2_YEAST (P37012) Phosphoglucomutase 2 (EC 5.4.2.2) (Glucose p... 30 9.9
>PTC1_CYNPY (O42335) Patched protein homolog 1 (PTC1) (Fragment)
Length = 257
Score = 33.9 bits (76), Expect = 0.69
Identities = 21/75 (28%), Positives = 40/75 (53%), Gaps = 2/75 (2%)
Query: 161 DSLSELLRHASLQSVAGLLSNLRSHDQISSIFGLLHSDLHEERAAAVLEYMSSMVASVAP 220
+S LLR +LQ++ L++ + ++ +LH + +E++AAA+LE M V
Sbjct: 112 NSNGTLLRAQALQTMFQLMTPKQMYEHFRGYEDVLHINWNEDKAAAILEAWQRMYVEV-- 169
Query: 221 LHHSAERGNLENSLS 235
+H S + + + LS
Sbjct: 170 VHQSVPQNSTQKVLS 184
>TOP2_PEA (O24308) DNA topoisomerase II (EC 5.99.1.3) (PsTopII)
Length = 1462
Score = 33.5 bits (75), Expect = 0.90
Identities = 19/54 (35%), Positives = 29/54 (53%), Gaps = 7/54 (12%)
Query: 324 GRRSLDDNSSEAAPISSGKKENSEMG-------EIIYFRDSDDEMPDSDEDPDD 370
G +S S E ++GKK+N++ G I+ DSD+E+ D D+D DD
Sbjct: 1244 GEKSQAMESEEVQQKAAGKKQNNKRGGAKKKSSTIVLESDSDNEVNDVDDDDDD 1297
>CN3B_RAT (Q63085) cGMP-inhibited 3',5'-cyclic phosphodiesterase B (EC
3.1.4.17) (Cyclic GMP inhibited phosphodiesterase B)
(CGI-PDE B) (CGIPDE1)
Length = 1108
Score = 32.7 bits (73), Expect = 1.5
Identities = 16/43 (37%), Positives = 25/43 (57%)
Query: 331 NSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
NS +AA + G+ +E G+ D DD+ D D+D D++LD
Sbjct: 983 NSYDAAGLLPGQWIEAEEGDDTESDDDDDDDDDDDDDDDEELD 1025
>SOMA_XENLA (P12855) Somatotropin A precursor (Growth hormone A)
(GH-A)
Length = 214
Score = 32.3 bits (72), Expect = 2.0
Identities = 31/128 (24%), Positives = 56/128 (43%), Gaps = 14/128 (10%)
Query: 87 SNNNWIHILDCYTDPLGW---KEKPRKSQNVTNPSHQVSLATTSYKTVKDLDKLFSVITE 143
SN N H++ CY++ + + K+ + ++ ++L + V+ L+K+FS
Sbjct: 68 SNKN-SHVVSCYSETIPYPTDKDNTHQKSDLELLRFSLNLIQSWLNPVQALNKVFSNNLV 126
Query: 144 LGRGLIGENKARFCVAIDSLSELLRHASLQSVAGLLS-------NLRSHDQISSIFGLL- 195
G + E I +L + L S +S L NLRS D + ++GLL
Sbjct: 127 FGSSDVYERLKYLEEGIQALMQELEDGSFRSFPFLRPPYERFDINLRSDDALVKVYGLLS 186
Query: 196 --HSDLHE 201
D+H+
Sbjct: 187 CFKKDMHK 194
>RGSC_RAT (O08774) Regulator of G-protein signaling 12 (RGS12)
Length = 1387
Score = 32.0 bits (71), Expect = 2.6
Identities = 20/63 (31%), Positives = 33/63 (51%), Gaps = 4/63 (6%)
Query: 158 VAIDSLSELLRHASLQSVAGLLSNLRSHDQISSI--FGLLHS--DLHEERAAAVLEYMSS 213
+ + S S L H SLQ++ G + LR+ +I S+ ++H L +RA V EY +
Sbjct: 237 IELPSTSSNLEHDSLQAIRGCMRRLRAEQKIHSLVTMKVMHDCVQLVTDRAGVVAEYPAE 296
Query: 214 MVA 216
+A
Sbjct: 297 KLA 299
>GPD2_ARATH (Q9FY99) Glucose-6-phosphate 1-dehydrogenase 2,
chloroplast precursor (EC 1.1.1.49) (G6PD2) (G6PDH2)
Length = 596
Score = 32.0 bits (71), Expect = 2.6
Identities = 25/80 (31%), Positives = 37/80 (46%), Gaps = 5/80 (6%)
Query: 210 YMSSMVASVAPLHHSAERGNLENSLSEQNFTKGRLNVRFKRRNGRVRVTCEEFKVEAGGI 269
Y S +AS +P++ R S S Q L VRF+R++GR V ++ G I
Sbjct: 13 YHSGYLASFSPVNGDRHRSLSFLSASPQGLNPLDLCVRFQRKSGRASVFMQD-----GAI 67
Query: 270 SLTSVSSADGATVAGLLPKV 289
S SS ++ GL +V
Sbjct: 68 VTNSNSSESKTSLKGLKDEV 87
>YSNF_BACSU (P94560) Stress response protein ysnF
Length = 286
Score = 31.6 bits (70), Expect = 3.4
Identities = 13/51 (25%), Positives = 31/51 (60%), Gaps = 6/51 (11%)
Query: 293 LQLSEKEKTDRAKVVLPFEHQGTGRPIQIYDGRRSLDDNSSEAAPISSGKK 343
+++ ++ KT++ + +P H +IY RR +D+N ++AAP++ ++
Sbjct: 166 VEIGKEVKTEKRDMDIPVRHD------EIYVERRPVDENKTDAAPVNDSEE 210
>UCRH_YEAST (P00127) Ubiquinol-cytochrome C reductase complex 17 kDa
protein (EC 1.10.2.2) (Mitochondrial hinge protein)
(Complex III polypeptide VI)
Length = 147
Score = 31.6 bits (70), Expect = 3.4
Identities = 15/45 (33%), Positives = 23/45 (50%), Gaps = 5/45 (11%)
Query: 329 DDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
DDN + G+++ E G+ + +DE D D+D DDD D
Sbjct: 28 DDNEQHEEKAAEGEEKEEENGD-----EDEDEDEDEDDDDDDDED 67
>STRQ_STRPY (P96471) Streptokinase precursor
Length = 440
Score = 31.6 bits (70), Expect = 3.4
Identities = 16/56 (28%), Positives = 25/56 (44%)
Query: 312 HQGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDED 367
H T R I +Y G+R +N+S + E Y RD+ +PD+ +D
Sbjct: 384 HDDTNRIITVYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRDTGTPIPDNPKD 439
>PTVB_ECOLI (P32154) PTS system, fructose-like-1 IIBC component
(Phosphotransferase enzyme II, BC component) (EC
2.7.1.69)
Length = 483
Score = 31.6 bits (70), Expect = 3.4
Identities = 20/81 (24%), Positives = 44/81 (53%), Gaps = 2/81 (2%)
Query: 140 VITELGRGLIGENKARFCVAIDSLSELLRHASLQSVAGLLSNLRSHDQISSIFGLLHSDL 199
VI GRGL G+++ARF A + E+ +L+++ + S L ++ Q+ + +
Sbjct: 63 VILATGRGLSGDDRARF--AGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGK 120
Query: 200 HEERAAAVLEYMSSMVASVAP 220
E ++ +V+ ++ + V++ P
Sbjct: 121 QEVQSGSVMSHLMAGVSAALP 141
>NUCL_MESAU (P08199) Nucleolin (Protein C23)
Length = 713
Score = 31.6 bits (70), Expect = 3.4
Identities = 34/155 (21%), Positives = 60/155 (37%), Gaps = 8/155 (5%)
Query: 218 VAPLHHSAERGNLENSLSEQNFTKGRLNVRFKRRNGRVRVTCEEFKVEAGGISLTSVSSA 277
+AP E + + +SE+ V ++ G+ KV +V +
Sbjct: 16 MAPPPKEVEEDSEDEEMSEEEDDSSGEEVVIPQKKGKKATATPAKKVVVSQTKKVAVPTP 75
Query: 278 DGATVAGLLPKVQFNLQLSEKEKTDRAKVVLPFEHQGT-GRPIQIYDGRRSLDDNSSEAA 336
A A + P + ++K T V P + T + + G++ + A
Sbjct: 76 --AKKAAVTPGKKAAATPAKKAVTPAKAVATPGKKGATQAKALVATPGKKGAVTPAKGAK 133
Query: 337 PISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
+ KKE+S+ E D DD+ DSDED +D+
Sbjct: 134 NGKNAKKEDSDEDE-----DDDDDEDDSDEDEEDE 163
>NUOG_CANBF (Q7VRV7) NADH-quinone oxidoreductase chain 3 (EC
1.6.99.5) (NADH dehydrogenase I, chain G) (NDH-1, chain
G)
Length = 941
Score = 31.2 bits (69), Expect = 4.5
Identities = 19/64 (29%), Positives = 32/64 (49%), Gaps = 5/64 (7%)
Query: 119 HQVSLATTSYKTVKDLDKLFSVITELGRGLIGENKARFCVAIDSLSELLRHASLQSVAGL 178
H V+ S T K +D +F+ +++ G A F I ++L RHAS+ + +
Sbjct: 545 HDVNSIGLSMMTSKSVDDVFNTLSDTNNG-----NAVFNTVIILENDLYRHASVAKINKM 599
Query: 179 LSNL 182
LSN+
Sbjct: 600 LSNI 603
>TUBE_DROVI (Q08171) Tube protein
Length = 514
Score = 30.8 bits (68), Expect = 5.8
Identities = 16/54 (29%), Positives = 25/54 (45%), Gaps = 3/54 (5%)
Query: 319 IQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDDL 372
+ I G LD N ++A +N+ G D D++ D D+D DDD+
Sbjct: 401 LNIASGAGELDGNGAKAR---GDNADNNSSGTNSLSNDDDEQKEDDDDDDDDDV 451
>TOP1_USTMA (P41511) DNA topoisomerase I (EC 5.99.1.2)
Length = 1019
Score = 30.8 bits (68), Expect = 5.8
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 5/60 (8%)
Query: 319 IQIYDGRRSLDDNSSEA-----APISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
+Q G + DDN ++ AP+++ K+++ + D DDE D DE+ DDD D
Sbjct: 73 VQKRSGSSNNDDNDDDSDSDSDAPLTALVKKSNGSDDDEDDDDDDDEGDDDDEEDDDDDD 132
>TPR_HUMAN (P12270) Nucleoprotein TPR
Length = 2349
Score = 30.4 bits (67), Expect = 7.6
Identities = 18/63 (28%), Positives = 31/63 (48%), Gaps = 6/63 (9%)
Query: 311 EHQGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDD 370
+ + T + +QI G D ++ ++ GK G+ + DSDDE D ++D DD
Sbjct: 1908 DSEETSQSLQIDLGPLQSDQQTTTSSQDGQGK------GDDVIVIDSDDEEEDEEDDDDD 1961
Query: 371 DLD 373
+ D
Sbjct: 1962 EDD 1964
>SIS2_CANTR (Q12600) SIS2 protein (Halotolerance protein HAL3)
Length = 531
Score = 30.4 bits (67), Expect = 7.6
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 4/45 (8%)
Query: 329 DDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
D++ EA K+N + II D DD+ D D+D DDD D
Sbjct: 464 DEDEDEA----DDSKDNIDESAIIDDDDDDDDDDDDDDDDDDDDD 504
>RPIA_ARATH (Q9ZU38) Probable-ribose 5-phosphate isomerase (EC
5.3.1.6) (Phosphoriboisomerase)
Length = 265
Score = 30.4 bits (67), Expect = 7.6
Identities = 20/73 (27%), Positives = 41/73 (55%), Gaps = 9/73 (12%)
Query: 127 SYKTVKDLDKLFSVITELGRGLIGENKARFCVAIDSLSELLRHASLQSVAGLLSNLRSHD 186
+YK V+ ++ ++ LG G ++ A+D + ELLR L+++ G+ ++ ++ +
Sbjct: 41 AYKAVEFVES--GMVLGLGTGSTAKH------AVDRIGELLRQGKLENIVGIPTSKKTQE 92
Query: 187 QISSIFGLLHSDL 199
Q S+ G+ SDL
Sbjct: 93 QALSL-GIPLSDL 104
>UBC_ASFM2 (P25869) Ubiquitin-conjugating enzyme E2-21 kDa (EC
6.3.2.19) (Ubiquitin-protein ligase) (Ubiquitin carrier
protein)
Length = 213
Score = 30.0 bits (66), Expect = 9.9
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 11/58 (18%)
Query: 325 RRSLDDNSSE-----------AAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
++SLD+ S+E P+ S E+ EM + Y DDE + DE+ DD+
Sbjct: 156 KKSLDECSAEDIEYFKNVPVNVLPVPSDDYEDEEMEDGTYILTYDDEDEEEDEEMDDE 213
>SELA_HAEDU (Q7VKC2) L-seryl-tRNA(Sec) selenium transferase (EC
2.9.1.1) (Cysteinyl-tRNA(Sec) selenium transferase)
(Selenocysteine synthase) (Selenocysteinyl-tRNA(Sec)
synthase)
Length = 460
Score = 30.0 bits (66), Expect = 9.9
Identities = 31/95 (32%), Positives = 45/95 (46%), Gaps = 18/95 (18%)
Query: 160 IDSLSE-LLRHASL---QSVAGLLS----NLRSHDQIS--SIFGLLHSDLHEERAAAVLE 209
IDSL + +L H SL Q LLS NL+ QI+ +F L + LH A+
Sbjct: 40 IDSLRQHILTHQSLPVDQPADNLLSLIATNLQQTQQIAMKKVFNLTGTILHTNLGRAIWS 99
Query: 210 YMSSMVASVAPLHHSA--------ERGNLENSLSE 236
+ AS A HHSA +R + +N++S+
Sbjct: 100 EAAITAASDAMRHHSALEFDIEAGKRSHRDNTISQ 134
>PGM2_YEAST (P37012) Phosphoglucomutase 2 (EC 5.4.2.2) (Glucose
phosphomutase 2) (PGM 2)
Length = 569
Score = 30.0 bits (66), Expect = 9.9
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 7/42 (16%)
Query: 112 QNVTNPSHQVSLATTSYKTVKDLDKLFSVITELGRGLIGENK 153
++VTN ++S TSYK +KD EL G IG+NK
Sbjct: 143 ESVTNAIWEISKKLTSYKIIKD-------FPELDLGTIGKNK 177
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.314 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,233,438
Number of Sequences: 164201
Number of extensions: 1642341
Number of successful extensions: 4860
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 4763
Number of HSP's gapped (non-prelim): 63
length of query: 374
length of database: 59,974,054
effective HSP length: 112
effective length of query: 262
effective length of database: 41,583,542
effective search space: 10894888004
effective search space used: 10894888004
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0346.3