BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0344.4
(242 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
VMP_SOCMV (P15631) Movement protein (Cell-TO-cell transport prot... 49 8e-06
VMP_CERV (P05396) Movement protein (Cell-TO-cell transport protein) 35 0.21
V51K_ACLSP (P27739) 50.8 kDa protein (ORF2) 34 0.28
V51K_ACLSA (P54892) 50.4 kDa protein (ORF2) 33 0.47
MOVP_TMOB (Q83485) Movement protein (Cell-to-cell transport prot... 31 3.1
VS06_ROTBS (Q00734) VP6 protein (Major capsid protein) (Major in... 30 5.2
RPOC_HAEDU (Q7VKL8) DNA-directed RNA polymerase beta' chain (EC ... 30 5.2
SAP4_YEAST (P53036) SIT4-associating protein SAP4 30 6.8
RPOC_HAEIN (P43739) DNA-directed RNA polymerase beta' chain (EC ... 29 8.9
>VMP_SOCMV (P15631) Movement protein (Cell-TO-cell transport
protein) (ORF IA)
Length = 303
Score = 49.3 bits (116), Expect = 8e-06
Identities = 42/152 (27%), Positives = 75/152 (48%), Gaps = 6/152 (3%)
Query: 72 SRIVNQHVRKYRDLHIGLVQLAVKPLTRLRYCDLPILICLRDKRMLRFHESVLAVLECNI 131
S+I + R R +HI +Q+ +K T L+ D P+ + LRD R+L ES +AV N+
Sbjct: 89 SKIEEKQRRLIRYVHISTLQVLIKS-TFLKGLDTPLELTLRDNRLLNLEESKIAVGHGNL 147
Query: 132 QNGPAYFNCFPSYPVCLRDPHVTTCVDLDIKIPNEMFI-DGIPPVSIMYRFCYKVTDNLS 190
+ G F+ + L+D + + L+ K F+ +G SI YR Y ++++
Sbjct: 148 KYGKMKFDVNLQLGLSLKDLDLDRSIILNYKFLRRNFMKEGNHAFSISYRINYALSNSHH 207
Query: 191 GI--KAVRDYTVNE--TEVTEINAENSSRITR 218
+ K ++E +EV E+ S++T+
Sbjct: 208 SVEFKQKEKIYIDELFSEVLELKHPVFSKLTK 239
>VMP_CERV (P05396) Movement protein (Cell-TO-cell transport protein)
Length = 319
Score = 34.7 bits (78), Expect = 0.21
Identities = 34/145 (23%), Positives = 62/145 (42%), Gaps = 8/145 (5%)
Query: 73 RIVNQHVRK-YRDLHIGLVQLAVKPLTRLRYCDLPILICLRDKRMLRFHESVLAVLECNI 131
+I ++ VR D+H G +++ +K R + PI + L D R+ +S+L N+
Sbjct: 106 KIKDEKVRSTISDIHFGAIKVLIKARFR-EGINSPIKMALIDDRITDRQDSILGAAHGNL 164
Query: 132 QNGPAYFNCFPSYPVCLRDPHV-TTCVDLDIKIPNEMFIDGIPPVSIMYRFCYKVTDNLS 190
G F +P Y + D + T + N++ G SI Y Y + ++
Sbjct: 165 VYGKFMFTVYPKYTTSILDQRLDRTLAFIHHFERNDLMRKGDKVFSITYLVAYALANSHH 224
Query: 191 GIKAVRDYTVNET-EVTEINAENSS 214
I DY + E+ ++ +E S
Sbjct: 225 SI----DYKEKDAIEIDDVFSEIGS 245
>V51K_ACLSP (P27739) 50.8 kDa protein (ORF2)
Length = 460
Score = 34.3 bits (77), Expect = 0.28
Identities = 30/131 (22%), Positives = 57/131 (42%), Gaps = 5/131 (3%)
Query: 67 ISILDSRIVNQHVRKYRD---LHIGLVQLAVKPLTRLRYCDLPILICLRDKRMLRFHESV 123
I ++ S V +RK +H G + +++ L R + + + D R F +++
Sbjct: 66 IPVIPSSEVQAVLRKRESTNYVHWGALSISIDALFR-KNAGVSGWCYVYDNRWETFEQAM 124
Query: 124 LAVLECNIQNGPAYFNCFPSYPVCLRDPHVTTCVDLDIKIPNEMFIDGIPPVSI-MYRFC 182
L N+ +G A P++PV L DP ++ + + + N F P+S+ + C
Sbjct: 125 LQKFHFNLDSGSATLVTSPNFPVSLDDPGLSNSISVAVMFENLNFKFESYPISVRVGNMC 184
Query: 183 YKVTDNLSGIK 193
LS +K
Sbjct: 185 RFFDSFLSSVK 195
>V51K_ACLSA (P54892) 50.4 kDa protein (ORF2)
Length = 457
Score = 33.5 bits (75), Expect = 0.47
Identities = 26/114 (22%), Positives = 51/114 (43%), Gaps = 4/114 (3%)
Query: 67 ISILDSRIVNQHVRKYRD---LHIGLVQLAVKPLTRLRYCDLPILICLRDKRMLRFHESV 123
I ++ S V +RK +H G + +++ L R + + + D R F +++
Sbjct: 64 IPVIPSSEVQAVLRKRESTNYVHWGALSISIDALFR-KNAGVSGWCYVYDNRWETFEQAM 122
Query: 124 LAVLECNIQNGPAYFNCFPSYPVCLRDPHVTTCVDLDIKIPNEMFIDGIPPVSI 177
L N+ +G A P++PV L DP ++ + + + N F P+S+
Sbjct: 123 LQKFRFNLDSGSATLVTSPNFPVSLDDPGLSNSISVAVMFENLNFKLESYPISV 176
>MOVP_TMOB (Q83485) Movement protein (Cell-to-cell transport
protein) (30 kDa protein)
Length = 274
Score = 30.8 bits (68), Expect = 3.1
Identities = 42/193 (21%), Positives = 83/193 (42%), Gaps = 16/193 (8%)
Query: 31 KIRTNQVYKTSIFEFNPDYEIKTVEKSFAVQGPYQRISILDSRIVNQHVRKYRDLHIGLV 90
K+ ++ S F + TV+K A + IS +D + + V+ ++ ++ LV
Sbjct: 13 KLSKSEEVLPSAFTRMKSVRVSTVDKIMAKEN--DNISEVD---LLKGVKLVKNGYVCLV 67
Query: 91 QLAVKPLTRLR-YCDLPILICLRDKRMLRFHESVLAVLECNIQNGPAYFNCFPSYPVCLR 149
L V L C + ICL DKRM R +E+ L F P+Y + +
Sbjct: 68 GLVVSGEWNLPDNCRGGVSICLIDKRMQRHNEATLGSYTTKASKKNFSFKLIPNYSITSQ 127
Query: 150 DPHVTTCVDLDIKIPNEMFIDGIPPVSIMY-RFCYKVTDNLSGIKAVRDYTVNETEVTEI 208
D ++ + I +G P+S+ + C +N+ K +R+ +VT +
Sbjct: 128 DAERRPW-EVMVNIRGVAMSEGWCPLSLEFVSVCIVHKNNVR--KGLRE------KVTAV 178
Query: 209 NAENSSRITRKIL 221
+ +++ +T +++
Sbjct: 179 SEDDAIELTEEVV 191
>VS06_ROTBS (Q00734) VP6 protein (Major capsid protein) (Major
internal structural protein)
Length = 395
Score = 30.0 bits (66), Expect = 5.2
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 7/64 (10%)
Query: 16 NDSNLEQKLQNWSIPKIRTNQVYKTSIFEFNPDYEIKTVEKSFAVQGPYQR-------IS 68
N+ L ++N I +RT Y+ +I P + V++ F V GPY +S
Sbjct: 265 NNGQLVDMVRNMGIATVRTFDSYRITIDMIRPAAMTQYVQQLFPVGGPYSHQAAYMLTLS 324
Query: 69 ILDS 72
+LD+
Sbjct: 325 VLDA 328
Score = 29.6 bits (65), Expect = 6.8
Identities = 14/46 (30%), Positives = 23/46 (49%), Gaps = 3/46 (6%)
Query: 16 NDSNLEQKLQNWSIPKIRTNQVYK---TSIFEFNPDYEIKTVEKSF 58
N +N Q ++NWS R N VY+ +FE+ Y + ++ F
Sbjct: 128 NFNNSSQSIKNWSAQSRRENPVYEYKNPMVFEYRNSYILHRADQQF 173
>RPOC_HAEDU (Q7VKL8) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta' chain)
(RNA polymerase beta' subunit)
Length = 1420
Score = 30.0 bits (66), Expect = 5.2
Identities = 20/55 (36%), Positives = 30/55 (54%), Gaps = 8/55 (14%)
Query: 189 LSGIKAVRDYTVNETEV------TEINAENSSRITRKILKHDEIT--YPQEWLQG 235
L G+ AV DY VNE + +IN ++ I R++L+ IT Y E+L+G
Sbjct: 1223 LRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLRKAVITNAYDSEFLEG 1277
>SAP4_YEAST (P53036) SIT4-associating protein SAP4
Length = 818
Score = 29.6 bits (65), Expect = 6.8
Identities = 14/36 (38%), Positives = 19/36 (51%)
Query: 5 TLNQHANNSEENDSNLEQKLQNWSIPKIRTNQVYKT 40
TL NN++ NDSN QK + K N++Y T
Sbjct: 502 TLEDKCNNNDSNDSNDNQKQKKNIKKKFHDNELYST 537
>RPOC_HAEIN (P43739) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta' chain)
(RNA polymerase beta' subunit)
Length = 1415
Score = 29.3 bits (64), Expect = 8.9
Identities = 19/55 (34%), Positives = 31/55 (55%), Gaps = 8/55 (14%)
Query: 189 LSGIKAVRDYTVNET------EVTEINAENSSRITRKILKHDEIT--YPQEWLQG 235
L G++AV +Y VNE + +IN ++ I R++L+ IT Y E+L+G
Sbjct: 1223 LRGVRAVTEYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKAVITKAYDSEFLEG 1277
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.137 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,232,661
Number of Sequences: 164201
Number of extensions: 1112034
Number of successful extensions: 2393
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2387
Number of HSP's gapped (non-prelim): 10
length of query: 242
length of database: 59,974,054
effective HSP length: 107
effective length of query: 135
effective length of database: 42,404,547
effective search space: 5724613845
effective search space used: 5724613845
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0344.4