BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0335a.7
         (632 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

CO3_HUMAN (P01024) Complement C3 precursor [Contains: C3a anaphy...    34  1.3
CO3_CAVPO (P12387) Complement C3 precursor [Contains: C3a anaphy...    32  3.9
UBP8_MOUSE (Q80U87) Ubiquitin carboxyl-terminal hydrolase 8 (EC ...    32  6.6
EFL4_MOUSE (P60882) Multiple EGF-like-domain protein 4                 31  8.6
EFL4_HUMAN (Q7Z7M0) Multiple EGF-like-domain protein 4 (Multiple...    31  8.6
CYG1_BOVIN (P16068) Guanylate cyclase soluble, beta-1 chain (EC ...    31  8.6

>CO3_HUMAN (P01024) Complement C3 precursor [Contains: C3a
           anaphylatoxin]
          Length = 1663

 Score = 33.9 bits (76), Expect = 1.3
 Identities = 30/107 (28%), Positives = 45/107 (42%), Gaps = 14/107 (13%)

Query: 187 RFSNELRKRAAGNTCKINF-ESPRSTLFPRFSRFYMCLDGCKRGFLAGCRPFIGLDGCHL 245
           ++  ELRK      C+    E+P      R +RF    + CK+ FL  C     L   H 
Sbjct: 685 KYPKELRK-----CCEDGMRENPMRFSCQRRTRFISLGEACKKVFLDCCNYITELRRQHA 739

Query: 246 KTKHRGILLAAVARD--ANEQYFPLAFGVVESETKDSWMWFMELLMD 290
           +  H G+  + +  D  A E        V  SE  +SW+W +E L +
Sbjct: 740 RASHLGLARSNLDEDIIAEENI------VSRSEFPESWLWNVEDLKE 780


>CO3_CAVPO (P12387) Complement C3 precursor [Contains: C3a
           anaphylatoxin]
          Length = 1666

 Score = 32.3 bits (72), Expect = 3.9
 Identities = 23/92 (25%), Positives = 41/92 (44%), Gaps = 7/92 (7%)

Query: 206 ESPRSTLFPRFSRFYMCLDGCKRGFLAGCRPFIGLDGCHLKTKHRGILLAAVARDANEQY 265
           E+P      R +R+    + C + FL  C     L   H + ++ G+  +    D +E  
Sbjct: 705 ENPMQFSCQRRARYVSLGEACVKAFLDCCTYMAQLRQQHRREQNLGLARS----DMDEDI 760

Query: 266 FPLAFGVVESETKDSWMWFMELLMDDIDPERS 297
            P    +  S+  +SW+W +E L    +PER+
Sbjct: 761 IPEEDIISRSQFPESWLWTIEELK---EPERN 789


>UBP8_MOUSE (Q80U87) Ubiquitin carboxyl-terminal hydrolase 8 (EC
           3.1.2.15) (Ubiquitin thiolesterase 8)
           (Ubiquitin-specific processing protease 8)
           (Deubiquitinating enzyme 8) (mUBPy)
          Length = 1080

 Score = 31.6 bits (70), Expect = 6.6
 Identities = 19/91 (20%), Positives = 43/91 (46%), Gaps = 5/91 (5%)

Query: 541 YENYVTPLPGPNQWIVTPHDPVCILPPMYKRSAGRPK-----KLRRRDPYENDPNPTKLQ 595
           +  ++ P+ G  ++  +P + V + PP    S+  P      K++ + P E D  P+KL+
Sbjct: 617 WAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDREPSKLK 676

Query: 596 RGGATWKCSRCKQYGHNKRGCKNPVPEEQSE 626
           R  ++   ++  Q    +R    P+   +++
Sbjct: 677 RSYSSPDITQALQEEEKRRPAVTPMVNRENK 707


>EFL4_MOUSE (P60882) Multiple EGF-like-domain protein 4
          Length = 2330

 Score = 31.2 bits (69), Expect = 8.6
 Identities = 21/86 (24%), Positives = 33/86 (37%), Gaps = 1/86 (1%)

Query: 499  TCRFWELNGFPCRHAASALTFNGNDPKQFVDDCYKREAYNATYENYVTPLPGPNQWIVTP 558
            TC   +  G PC++     T  G+ P     DCYK +        + +PL G   + +  
Sbjct: 1873 TCNEQDGTGCPCQNNTETGTCQGSSPSDR-RDCYKYQCAKCRESFHGSPLGGQQCYRLIS 1931

Query: 559  HDPVCILPPMYKRSAGRPKKLRRRDP 584
             +  C L P  + +     K R   P
Sbjct: 1932 VEQECCLDPTSQTNCFHEPKRRALGP 1957


>EFL4_HUMAN (Q7Z7M0) Multiple EGF-like-domain protein 4 (Multiple
            epidermal growth factor-like domains 8)
          Length = 2386

 Score = 31.2 bits (69), Expect = 8.6
 Identities = 21/86 (24%), Positives = 33/86 (37%), Gaps = 1/86 (1%)

Query: 499  TCRFWELNGFPCRHAASALTFNGNDPKQFVDDCYKREAYNATYENYVTPLPGPNQWIVTP 558
            TC   +  G PC++     T  G+ P     DCYK +        + +PL G   + +  
Sbjct: 1929 TCNEQDGTGCPCQNNTETGTCQGSSPSDR-RDCYKYQCAKCRESFHGSPLGGQQCYRLIS 1987

Query: 559  HDPVCILPPMYKRSAGRPKKLRRRDP 584
             +  C L P  + +     K R   P
Sbjct: 1988 VEQECCLDPTSQTNCFHEPKRRALGP 2013


>CYG1_BOVIN (P16068) Guanylate cyclase soluble, beta-1 chain (EC
           4.6.1.2) (GCS-beta-1) (Soluble guanylate cyclase small
           subunit)
          Length = 619

 Score = 31.2 bits (69), Expect = 8.6
 Identities = 18/44 (40%), Positives = 22/44 (49%), Gaps = 3/44 (6%)

Query: 43  LGMDFSSLLQFKDALHDHFVTIGREFEYMKNDSIRCRVKCRSKG 86
           LG +    LQ  DALHDH  TI   +  M+  S RC    + KG
Sbjct: 90  LGSNVREFLQNLDALHDHLATI---YPGMRAPSFRCTDADKGKG 130


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.323    0.137    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,953,918
Number of Sequences: 164201
Number of extensions: 3478475
Number of successful extensions: 6655
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 6655
Number of HSP's gapped (non-prelim): 6
length of query: 632
length of database: 59,974,054
effective HSP length: 116
effective length of query: 516
effective length of database: 40,926,738
effective search space: 21118196808
effective search space used: 21118196808
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 69 (31.2 bits)