BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0335a.6
(109 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ST11_YEAST (P23561) Serine/threonine-protein kinase STE11 (EC 2.... 29 2.2
SHK2_SCHPO (Q10056) Serine/threonine-protein kinase shk2 (EC 2.7... 27 6.5
RUVB_MYCPU (Q98PR1) Holliday junction DNA helicase ruvB 27 6.5
GPMI_HELPJ (Q9ZKM7) 2,3-bisphosphoglycerate-independent phosphog... 27 6.5
RTN_ECOLI (P76446) Rtn protein 27 8.5
PRSN_PIERA (Q9U8Q4) Pierisin (EC 2.4.2.-) (NAD--DNA ADP-ribosylt... 27 8.5
PRSN_PIEBR (Q9GV36) Pierisin (EC 2.4.2.-) (NAD--DNA ADP-ribosylt... 27 8.5
DD25_RAT (Q9QY16) ATP-dependent RNA helicase DDX25 (DEAD-box pro... 27 8.5
>ST11_YEAST (P23561) Serine/threonine-protein kinase STE11 (EC
2.7.1.37)
Length = 738
Score = 28.9 bits (63), Expect = 2.2
Identities = 18/64 (28%), Positives = 25/64 (38%), Gaps = 5/64 (7%)
Query: 9 LPSSFRWPRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRP-----EEDNPQ 63
L S W P +V Q T + + WS +V K PF D S + P+
Sbjct: 638 LQGSVFWMSPEVVKQTATTAKADIWSTGCVVIEMFTGKHPFPDFSQMQAIFKIGTNTTPE 697
Query: 64 LNSW 67
+ SW
Sbjct: 698 IPSW 701
>SHK2_SCHPO (Q10056) Serine/threonine-protein kinase shk2 (EC
2.7.1.37)
Length = 589
Score = 27.3 bits (59), Expect = 6.5
Identities = 19/96 (19%), Positives = 42/96 (42%), Gaps = 16/96 (16%)
Query: 15 WPRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFV---------------DGSLPRPEE 59
W P +V Q + + WS +++ ++N+ P++ +L +P
Sbjct: 475 WMAPEVVKQNEYGTKVDIWSLGIMIIEMIENEPPYLREDPIRALYLIAKNGTPTLKKPNL 534
Query: 60 DNPQLNSWIRNNNVVISWIFNAVSKDITTSMYSDTA 95
+ L S++ N+ + I IF A + ++ T + + A
Sbjct: 535 VSKNLKSFL-NSCLTIDTIFRATAAELLTHSFLNQA 569
>RUVB_MYCPU (Q98PR1) Holliday junction DNA helicase ruvB
Length = 315
Score = 27.3 bits (59), Expect = 6.5
Identities = 13/59 (22%), Positives = 33/59 (55%)
Query: 34 SRAVLVALSMKNKVPFVDGSLPRPEEDNPQLNSWIRNNNVVISWIFNAVSKDITTSMYS 92
S A +++ S+ +++ + G+L + D L + + N+++ +A++K+I +YS
Sbjct: 54 SLASIISNSLNSRIKYAQGNLLDKKTDILTLFASVEENDIIFVDEIHAINKNIEELLYS 112
>GPMI_HELPJ (Q9ZKM7) 2,3-bisphosphoglycerate-independent
phosphoglycerate mutase (EC 5.4.2.1)
(Phosphoglyceromutase) (BPG-independent PGAM) (iPGM)
Length = 491
Score = 27.3 bits (59), Expect = 6.5
Identities = 15/52 (28%), Positives = 25/52 (47%), Gaps = 1/52 (1%)
Query: 6 QSFLPSSFRWPRPRLVSQPLTESNYNTWSRAVLVALSMKNKVPFVDGSLPRP 57
+SF+ +FR R R + L + +N++ R L + P+ D S P P
Sbjct: 239 ESFIFINFRNDRAREIVSALGQKEFNSFKRQAFKKLHIATMTPY-DNSFPYP 289
>RTN_ECOLI (P76446) Rtn protein
Length = 518
Score = 26.9 bits (58), Expect = 8.5
Identities = 12/43 (27%), Positives = 24/43 (54%)
Query: 60 DNPQLNSWIRNNNVVISWIFNAVSKDITTSMYSDTASEIYHGL 102
+ P + W RN + S +F A++ ++T S++ + E Y G+
Sbjct: 143 NKPAIVIWYRNPLLKNSGVFAALNLNLTPSLFYSSRQEDYDGV 185
>PRSN_PIERA (Q9U8Q4) Pierisin (EC 2.4.2.-) (NAD--DNA
ADP-ribosyltransferase) (Pierisin-1)
Length = 850
Score = 26.9 bits (58), Expect = 8.5
Identities = 9/29 (31%), Positives = 20/29 (68%)
Query: 69 RNNNVVISWIFNAVSKDITTSMYSDTASE 97
R +N++++W NA SK++ Y+++ S+
Sbjct: 473 RKSNLLLTWDSNAASKEMVVRAYTESRSK 501
>PRSN_PIEBR (Q9GV36) Pierisin (EC 2.4.2.-) (NAD--DNA
ADP-ribosyltransferase) (Pierisin-2) (Pierisin-B)
Length = 850
Score = 26.9 bits (58), Expect = 8.5
Identities = 9/29 (31%), Positives = 20/29 (68%)
Query: 69 RNNNVVISWIFNAVSKDITTSMYSDTASE 97
R +N++++W NA SK++ Y+++ S+
Sbjct: 473 RKSNLLLTWDSNATSKEMVVRAYTESRSK 501
>DD25_RAT (Q9QY16) ATP-dependent RNA helicase DDX25 (DEAD-box
protein 25) (Gonadotropin-regulated testicular RNA
helicase)
Length = 483
Score = 26.9 bits (58), Expect = 8.5
Identities = 19/56 (33%), Positives = 26/56 (45%), Gaps = 10/56 (17%)
Query: 47 VPFVDGSLPRPEED----------NPQLNSWIRNNNVVISWIFNAVSKDITTSMYS 92
VP +DGSL E+D N LN IR + V S + KD ++ +YS
Sbjct: 40 VPNIDGSLNTEEDDDEDDVVDLAANSLLNKLIRQSLVESSHRVEVLQKDPSSPLYS 95
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.130 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,282,830
Number of Sequences: 164201
Number of extensions: 487758
Number of successful extensions: 1078
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1069
Number of HSP's gapped (non-prelim): 12
length of query: 109
length of database: 59,974,054
effective HSP length: 85
effective length of query: 24
effective length of database: 46,016,969
effective search space: 1104407256
effective search space used: 1104407256
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0335a.6