BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0327.8
(250 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AMPN_MANSE (P91885) Aminopeptidase N precursor (EC 3.4.11.2) (Mi... 34 0.29
RPOC_FUSNN (Q8RHI7) DNA-directed RNA polymerase beta' chain (EC ... 32 1.9
AHR_HUMAN (P35869) Aryl hydrocarbon receptor precursor (Ah recep... 31 2.5
HTPX_SHEON (Q8EDL5) Probable protease htpX homolog (EC 3.4.24.-) 31 3.2
HML2_HELRO (Q01622) Homeobox LOX2 protein (Fragment) 30 4.2
Y640_SYNY3 (P72958) Hypothetical protein sll0640 30 5.5
TBB3_DROME (P08841) Tubulin beta-3 chain (Beta-3 tubulin) 30 5.5
RPOC_BUCAP (P41185) DNA-directed RNA polymerase beta' chain (EC ... 30 5.5
AHR_MUSSP (Q8R4S2) Aryl hydrocarbon receptor precursor (Ah recep... 30 5.5
POL_CAEVC (P33459) Pol polyprotein [Contains: Protease (Retropep... 30 7.1
HTPX_FUSNN (Q8R664) Probable protease htpX homolog (EC 3.4.24.-) 30 7.1
ZNUA_BUCAP (Q8K9M5) High-affinity zinc uptake system protein znu... 29 9.3
RIP1_BRYDI (P33185) Ribosome-inactivating protein bryodin I prec... 29 9.3
>AMPN_MANSE (P91885) Aminopeptidase N precursor (EC 3.4.11.2)
(Microsomal aminopeptidase) (APN2)
Length = 942
Score = 34.3 bits (77), Expect = 0.29
Identities = 39/160 (24%), Positives = 66/160 (40%), Gaps = 33/160 (20%)
Query: 36 IASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQF--ILQTFKQIDQ- 92
I +S +PI+ + ++V +R GT+ E + F QQ + ++ F +ID+
Sbjct: 242 IENSQNPIYPIPFRVYSRP-----GTQNTAEFALEFGQQNMIALEEYTEFPYAFPKIDKA 296
Query: 93 ----FGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVIGILYHEAT 148
F +E+ +++ A+ + S V+ + I+ HE T
Sbjct: 297 AVPDFAAGAMENWGLVIY----REVALLVREGVTTTS---------VKQNIGRIICHENT 343
Query: 149 HIWQWFGNKEAPRG-----LTEGVADFMRLKA-GFAPPHW 182
H+ WFGN+ P L EG A+F A F P W
Sbjct: 344 HM--WFGNEVGPMSWTYTWLNEGFANFFENYATDFVRPQW 381
>RPOC_FUSNN (Q8RHI7) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta' chain)
(RNA polymerase beta' subunit)
Length = 1319
Score = 31.6 bits (70), Expect = 1.9
Identities = 20/62 (32%), Positives = 33/62 (52%), Gaps = 2/62 (3%)
Query: 79 ASQFILQTFKQI--DQFGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVR 136
A QFIL++ +Q+ DQ N +H+ +IV + I S ++FL+ IE D+
Sbjct: 1166 AEQFILESVQQVYRDQGVTVNDKHIEIIVKQMFRKVRIIDSGASLFLEDEVIEKRVVDLE 1225
Query: 137 NE 138
N+
Sbjct: 1226 NK 1227
>AHR_HUMAN (P35869) Aryl hydrocarbon receptor precursor (Ah
receptor) (AhR)
Length = 848
Score = 31.2 bits (69), Expect = 2.5
Identities = 16/55 (29%), Positives = 30/55 (54%)
Query: 95 GKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVIGILYHEATH 149
G N++ ++ ALNG +T+D +F S+ I+ + G +++VI +E H
Sbjct: 109 GLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIH 163
>HTPX_SHEON (Q8EDL5) Probable protease htpX homolog (EC 3.4.24.-)
Length = 287
Score = 30.8 bits (68), Expect = 3.2
Identities = 32/165 (19%), Positives = 70/165 (42%), Gaps = 15/165 (9%)
Query: 81 QFILQTFKQIDQFGGKNIEHVSVIVTA-LNGNSAAITSDNNIF-LDSNYIEGFSGDVRNE 138
+++L T + Q G + V++ + +N + + DN++ + + + G S D E
Sbjct: 76 RWLLDTVARQAQQAGIKMPEVAIYQSPEMNAFATGPSKDNSLVAVSTGLLYGMSQD---E 132
Query: 139 VIGILYHEATHIWQWFGNKEAPRGLTEGVADFMRLKAGFAPPHWVPRGTGSGWDEG---- 194
+ G+L HE +H+ L +GV + + A + S +EG
Sbjct: 133 IEGVLAHEVSHV---ANGDMVTLTLIQGVVNTFVIFAARVVAGIINNFVSSNDEEGEGLG 189
Query: 195 ---YVVTAYFLDYCNGLRDGFVAMLNAMMKDHYSDDFFVKLLGKS 236
Y+ + LD G+ + + ++++ +D+ +L GK+
Sbjct: 190 MFAYMAVVFVLDMLFGILASIIVAYFSRIREYRADEGAARLAGKN 234
>HML2_HELRO (Q01622) Homeobox LOX2 protein (Fragment)
Length = 429
Score = 30.4 bits (67), Expect = 4.2
Identities = 17/68 (25%), Positives = 35/68 (51%), Gaps = 7/68 (10%)
Query: 71 FTQQTIQLASQFILQTFKQIDQFGGKNIEHVSVIV-------TALNGNSAAITSDNNIFL 123
+ QQ L ++F + +QF ++++V++ T LN NS+ +++NI +
Sbjct: 53 YQQQQQLLLAEFDTDYKENAEQFADVSVQNVNISCSNSNNNNTDLNNNSSKCMNNSNIII 112
Query: 124 DSNYIEGF 131
D+N + F
Sbjct: 113 DNNSTDSF 120
>Y640_SYNY3 (P72958) Hypothetical protein sll0640
Length = 612
Score = 30.0 bits (66), Expect = 5.5
Identities = 20/51 (39%), Positives = 29/51 (56%), Gaps = 4/51 (7%)
Query: 24 MSPIIYLMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFEN--EIGIPFT 72
++P+ + MF+V A+S S + + YQ TN PGG RF + IG P T
Sbjct: 543 LNPLAF-MFVVTFAASNSFMSPIGYQ-TNTMVYGPGGYRFTDFARIGAPLT 591
>TBB3_DROME (P08841) Tubulin beta-3 chain (Beta-3 tubulin)
Length = 454
Score = 30.0 bits (66), Expect = 5.5
Identities = 23/82 (28%), Positives = 39/82 (47%), Gaps = 9/82 (10%)
Query: 123 LDSNYIEGFSGDVRNEVIGILYHEATHIWQWFGNKEAPRG----LTEGVADFMR---LKA 175
+DSN I D++ E + + Y+EA+ + + G K PR L G + +R
Sbjct: 30 IDSNGIYVGDSDLQLERVSVYYNEASAVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQ 89
Query: 176 GFAPPHWV--PRGTGSGWDEGY 195
F P ++V G G+ W +G+
Sbjct: 90 LFRPDNFVYGQSGAGNNWAKGH 111
>RPOC_BUCAP (P41185) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta' chain)
(RNA polymerase beta' subunit)
Length = 1413
Score = 30.0 bits (66), Expect = 5.5
Identities = 15/67 (22%), Positives = 34/67 (50%), Gaps = 2/67 (2%)
Query: 76 IQLASQFILQTFKQIDQFGGKNI--EHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSG 133
+Q +++I+ +++ + G I +H+ VI+ + + I S N+ FLD +E
Sbjct: 1226 VQAVTKYIVNEVQEVYRLQGVKINDKHIEVIIRQMLRKATVIKSGNSEFLDGEQVEFSRI 1285
Query: 134 DVRNEVI 140
+ N ++
Sbjct: 1286 KISNRIL 1292
>AHR_MUSSP (Q8R4S2) Aryl hydrocarbon receptor precursor (Ah
receptor) (AhR)
Length = 854
Score = 30.0 bits (66), Expect = 5.5
Identities = 15/54 (27%), Positives = 30/54 (54%)
Query: 96 KNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVIGILYHEATH 149
+N++ ++ ALNG +T+D +F S+ I+ + G +++VI +E H
Sbjct: 108 QNLQEGEFLLQALNGFVLVVTADALVFYASSTIQDYLGFQQSDVIHQSVYELIH 161
>POL_CAEVC (P33459) Pol polyprotein [Contains: Protease
(Retropepsin) (EC 3.4.23.-); Reverse
transcriptase/ribonuclease H (EC 2.7.7.49) (EC 3.1.26.4)
(RT); Integrase (IN)]
Length = 1109
Score = 29.6 bits (65), Expect = 7.1
Identities = 15/35 (42%), Positives = 20/35 (56%), Gaps = 1/35 (2%)
Query: 149 HIWQWFGNKEAPRGLTEGVADFMRL-KAGFAPPHW 182
H+ QW +E +GLTE + + K G APPHW
Sbjct: 167 HVPQWPLTEEKLKGLTEIIDKLVEEGKLGKAPPHW 201
>HTPX_FUSNN (Q8R664) Probable protease htpX homolog (EC 3.4.24.-)
Length = 309
Score = 29.6 bits (65), Expect = 7.1
Identities = 25/93 (26%), Positives = 38/93 (39%), Gaps = 16/93 (17%)
Query: 59 GGTRFENEIGIPFTQQTIQLASQFI-LQTFKQIDQFGGKNIEHVSVIVTALNGNSAAITS 117
GG E E T+ L SQ + LQ F +I + +I + T + NSA +
Sbjct: 83 GGASTEKE---KLVVDTVTLLSQKLDLQKFPEIGVYPSNDI---NAFATGASKNSAMVAV 136
Query: 118 DNNIFLDSNYIEGFSGDVRNEVIGILYHEATHI 150
+ N E+IG+L HE +H+
Sbjct: 137 SQGLLNSMN---------ETEIIGVLAHEMSHV 160
>ZNUA_BUCAP (Q8K9M5) High-affinity zinc uptake system protein znuA
precursor
Length = 317
Score = 29.3 bits (64), Expect = 9.3
Identities = 21/65 (32%), Positives = 28/65 (42%), Gaps = 1/65 (1%)
Query: 29 YLMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTFK 88
Y L + G A+ + VTN P GT EN +PF I+ S FI+
Sbjct: 30 YASILTVFKPLGFIAAAIAHNVTNVEVIPPNGTTVENYYLLPFDLIKIK-HSDFIILIGD 88
Query: 89 QIDQF 93
QI+ F
Sbjct: 89 QIEPF 93
>RIP1_BRYDI (P33185) Ribosome-inactivating protein bryodin I
precursor (EC 3.2.2.22) (rRNA N-glycosidase) (BD1)
Length = 290
Score = 29.3 bits (64), Expect = 9.3
Identities = 30/133 (22%), Positives = 53/133 (39%), Gaps = 7/133 (5%)
Query: 11 LSSCAALIYHWTKMSPIIYLMFLVIIASSGSPIHAVQYQVTNRATGT--PGGTRFENEIG 68
L S +Y++T S L+ L+ + + ++ Q+ R T P E
Sbjct: 155 LDSAITTLYYYTASSAASALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENN 214
Query: 69 IPFTQQTIQLASQFILQTFKQIDQFGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYI 128
+ IQ+AS Q + G N + VS+ N ++ +TS+ + L+ N I
Sbjct: 215 WSALSKQIQIASTNNGQFESPVVLIDGNN-QRVSIT----NASARVVTSNIALLLNRNNI 269
Query: 129 EGFSGDVRNEVIG 141
D+ +IG
Sbjct: 270 AAIGEDISMTLIG 282
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.139 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,753,847
Number of Sequences: 164201
Number of extensions: 1332842
Number of successful extensions: 2641
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 2638
Number of HSP's gapped (non-prelim): 14
length of query: 250
length of database: 59,974,054
effective HSP length: 108
effective length of query: 142
effective length of database: 42,240,346
effective search space: 5998129132
effective search space used: 5998129132
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0327.8