BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0327.6
(442 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CD2_RAT (P08921) T-cell surface antigen CD2 precursor (T-cell su... 35 0.30
ENAH_MOUSE (Q03173) Enabled protein homolog (NPC derived proline... 34 0.86
UBQ4_HUMAN (Q9NRR5) Ubiquilin 4 (Ataxin-1 ubiquitin-like interac... 32 3.3
PTK1_YEAST (P36002) Serine/threonine-protein kinase PTK1/STK1 (E... 32 4.3
RS19_LISMO (P66484) 30S ribosomal protein S19 31 5.6
RS19_LISMF (Q71WF0) 30S ribosomal protein S19 31 5.6
RS19_LISIN (P66485) 30S ribosomal protein S19 31 5.6
WS14_CAEEL (P41846) WBSCR14 protein homolog (Mlx interactor) 30 9.5
R3HD_HUMAN (Q15032) R3H domain protein 1 30 9.5
PO22_MOUSE (Q00196) POU domain, class 2, transcription factor 2 ... 30 9.5
PO22_HUMAN (P09086) POU domain, class 2, transcription factor 2 ... 30 9.5
>CD2_RAT (P08921) T-cell surface antigen CD2 precursor (T-cell
surface antigen T11/Leu-5) (LFA-2) (LFA-3 receptor)
(OX-34 antigen)
Length = 344
Score = 35.4 bits (80), Expect = 0.30
Identities = 25/86 (29%), Positives = 39/86 (45%), Gaps = 11/86 (12%)
Query: 46 RVRLRRGGDIHRVLARLPPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPPGNHI----- 100
R R R+G ++ +R+ ++ + P S+ P+ S APP PPG+H+
Sbjct: 233 RNRRRKGEELEIKASRMSTVERGPKPHSTQASAPASQ--NPVASQAPP-PPGHHLQTPGH 289
Query: 101 ---PLTHRAR*HLPKIPAPETEAGTH 123
P +HR R H PK P + H
Sbjct: 290 RPLPPSHRNREHQPKKRPPPSGTQVH 315
>ENAH_MOUSE (Q03173) Enabled protein homolog (NPC derived
proline-rich protein 1) (NDPP-1)
Length = 802
Score = 33.9 bits (76), Expect = 0.86
Identities = 21/58 (36%), Positives = 25/58 (42%)
Query: 63 PPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPETEA 120
PP+ A P P PLPS+ PP PP PL ++A P PAP A
Sbjct: 549 PPLPSGPAYASALPPPPGPPPPPPLPSTGPPPPPPPPPPLPNQAPPPPPPPPAPPLPA 606
>UBQ4_HUMAN (Q9NRR5) Ubiquilin 4 (Ataxin-1 ubiquitin-like
interacting protein A1U)
Length = 601
Score = 32.0 bits (71), Expect = 3.3
Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Query: 13 SGLERVRLTTQVRASE*GQQLNSLNRTQSRANLRVRLRRGGDIHRVLARL 62
SG +V+ T +VR + +QLNS+ ANL+ + GGDI+ + RL
Sbjct: 547 SGNSQVQ-TPEVRFQQQLEQLNSMGFINREANLQALIATGGDINAAIERL 595
>PTK1_YEAST (P36002) Serine/threonine-protein kinase PTK1/STK1 (EC
2.7.1.37)
Length = 649
Score = 31.6 bits (70), Expect = 4.3
Identities = 21/57 (36%), Positives = 26/57 (44%), Gaps = 6/57 (10%)
Query: 51 RGGDIHRVLARLPPIQHP--KASLGIREMQPFSR*PKP----LPSSAPPTPPGNHIP 101
RG I +A P +P K + IR M + P P L SS PP PP H+P
Sbjct: 532 RGFHIATDVAATTPTSNPFLKNRVPIRSMVDIAAHPSPTATVLASSPPPPPPATHVP 588
>RS19_LISMO (P66484) 30S ribosomal protein S19
Length = 92
Score = 31.2 bits (69), Expect = 5.6
Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 15/85 (17%)
Query: 287 QKIVKGNFEKSEHISKVKAAARSSRRTRTKSWRMDQD-----AEGNAAIFD------VVL 335
+ + KG F + KV+AAA S ++ K+W A++D V +
Sbjct: 3 RSLKKGPFVDDHLMKKVEAAAESEKKQVIKTWSRRSTIFPTFVGQTIAVYDGRKHVPVYV 62
Query: 336 HDNMTGLEVGAWKPT----GQTGDD 356
++M G ++G + PT G GDD
Sbjct: 63 QEDMVGHKLGEFAPTRTYRGHAGDD 87
>RS19_LISMF (Q71WF0) 30S ribosomal protein S19
Length = 92
Score = 31.2 bits (69), Expect = 5.6
Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 15/85 (17%)
Query: 287 QKIVKGNFEKSEHISKVKAAARSSRRTRTKSWRMDQD-----AEGNAAIFD------VVL 335
+ + KG F + KV+AAA S ++ K+W A++D V +
Sbjct: 3 RSLKKGPFVDDHLMKKVEAAAESEKKQVIKTWSRRSTIFPTFVGQTIAVYDGRKHVPVYV 62
Query: 336 HDNMTGLEVGAWKPT----GQTGDD 356
++M G ++G + PT G GDD
Sbjct: 63 QEDMVGHKLGEFAPTRTYRGHAGDD 87
>RS19_LISIN (P66485) 30S ribosomal protein S19
Length = 92
Score = 31.2 bits (69), Expect = 5.6
Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 15/85 (17%)
Query: 287 QKIVKGNFEKSEHISKVKAAARSSRRTRTKSWRMDQD-----AEGNAAIFD------VVL 335
+ + KG F + KV+AAA S ++ K+W A++D V +
Sbjct: 3 RSLKKGPFVDDHLMKKVEAAAESEKKQVIKTWSRRSTIFPTFVGQTIAVYDGRKHVPVYV 62
Query: 336 HDNMTGLEVGAWKPT----GQTGDD 356
++M G ++G + PT G GDD
Sbjct: 63 QEDMVGHKLGEFAPTRTYRGHAGDD 87
>WS14_CAEEL (P41846) WBSCR14 protein homolog (Mlx interactor)
Length = 1009
Score = 30.4 bits (67), Expect = 9.5
Identities = 18/68 (26%), Positives = 30/68 (43%)
Query: 40 QSRANLRVRLRRGGDIHRVLARLPPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPPGNH 99
Q ++N V L + R+L PP+ P + + Q P+ L S+ P P +H
Sbjct: 468 QQQSNFLVSLPHQSHVERILNNQPPLVPPPSRSNLLPTQNDPYLPQFLQSTQPTPQPQSH 527
Query: 100 IPLTHRAR 107
P+ +R
Sbjct: 528 DPMMAPSR 535
>R3HD_HUMAN (Q15032) R3H domain protein 1
Length = 971
Score = 30.4 bits (67), Expect = 9.5
Identities = 50/229 (21%), Positives = 90/229 (38%), Gaps = 20/229 (8%)
Query: 178 EDPQNVLPHRRSRHRALFHAHHQLEAHVQLEYTVGFHDGFIQVRARVDNIRLHVARLGFN 237
E P+ P S HR L H++ A+ L++ V G + + N R+ + +
Sbjct: 142 ESPRKKFPPMTSYHRMLL---HRVAAYFGLDHNVD-QSGKSVIVNKTSNTRIPDQKFNEH 197
Query: 238 KDGDDVDFIDEKHFPSRVRVWVGPEIGAAYVGGLSLGRSTENNEREVEIQKIVKGNFEKS 297
D + +++ R + + RS ERE E Q+ F +
Sbjct: 198 IKDDKGEDFQKRYILKRDNSSFDKDDNQVRIRLKDDRRSKSIEEREEEYQRARDRIFSQD 257
Query: 298 EHIS--------KVKAAARSSRRTRTKSWRMDQDAEGNAAIFDVVLHDNMTGLEVGAWKP 349
S +++ SS + R + +R+++DA G + H + T E+ +P
Sbjct: 258 SLCSQENYIIDKRLQDEDASSTQQRRQIFRVNKDASGRS----TNSHQSSTENELKYSEP 313
Query: 350 TGQTGDDPVHGMRGRYSGASRPFNKSG-SVVIAGDEYG--EEVGWRLSK 395
+ D +R ++ + SG SV+ GD G + +G RLSK
Sbjct: 314 RPWSSTDSDSSLRNLKPAVTKASSFSGISVLTRGDSSGSSKSIG-RLSK 361
>PO22_MOUSE (Q00196) POU domain, class 2, transcription factor 2
(Octamer-binding transcription factor 2) (Oct-2) (OTF-2)
(Lymphoid-restricted immunoglobulin octamer binding
protein NF-A2)
Length = 462
Score = 30.4 bits (67), Expect = 9.5
Identities = 30/95 (31%), Positives = 34/95 (35%), Gaps = 12/95 (12%)
Query: 85 KPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPETEAGTHIMDHGLTHTGISL-----QFL 139
K P S PT P I + P P P A H+ L TG L Q L
Sbjct: 47 KASPFSVSPTGPSTKIKAEDPSGDSAPAAPPPPQPAQPHLPQAQLMLTGSQLAGDIQQLL 106
Query: 140 Q-------PRLHRAPLLALRL*RAQRSWVTLLPIP 167
Q P H P L +AQ+S LLP P
Sbjct: 107 QLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLPTP 141
>PO22_HUMAN (P09086) POU domain, class 2, transcription factor 2
(Octamer-binding transcription factor 2) (Oct-2) (OTF-2)
(Lymphoid-restricted immunoglobulin octamer binding
protein NF-A2)
Length = 478
Score = 30.4 bits (67), Expect = 9.5
Identities = 30/95 (31%), Positives = 34/95 (35%), Gaps = 12/95 (12%)
Query: 85 KPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPETEAGTHIMDHGLTHTGISL-----QFL 139
K P S PT P I + P P P A H+ L TG L Q L
Sbjct: 47 KTSPFSVSPTGPSTKIKAEDPSGDSAPAAPLPPQPAQPHLPQAQLMLTGSQLAGDIQQLL 106
Query: 140 Q-------PRLHRAPLLALRL*RAQRSWVTLLPIP 167
Q P H P L +AQ+S LLP P
Sbjct: 107 QLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLPTP 141
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.141 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,033,541
Number of Sequences: 164201
Number of extensions: 2450790
Number of successful extensions: 8162
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 8147
Number of HSP's gapped (non-prelim): 22
length of query: 442
length of database: 59,974,054
effective HSP length: 113
effective length of query: 329
effective length of database: 41,419,341
effective search space: 13626963189
effective search space used: 13626963189
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 67 (30.4 bits)
Lotus: description of TM0327.6