BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0320b.5
(930 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergeni... 70 3e-11
PIF1_YEAST (P07271) DNA repair and recombination protein PIF1, m... 50 2e-05
PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1, m... 50 2e-05
EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 37 0.32
HELI_EHV1B (P28934) Probable helicase 35 0.92
PKSJ_BACSU (P40806) Putative polyketide synthase pksJ (PKS) 35 1.2
THDE_MOUSE (Q8K093) Thyrotropin-releasing hormone degrading ecto... 34 1.6
TA4B_HUMAN (Q92750) Transcription initiation factor TFIID 105 kD... 33 2.7
THDE_RAT (Q10836) Thyrotropin-releasing hormone degrading ectoen... 33 3.5
THDE_HUMAN (Q9UKU6) Thyrotropin-releasing hormone degrading ecto... 33 3.5
KITH_EHV4 (P24425) Thymidine kinase (EC 2.7.1.21) 33 4.6
YF19_METJA (Q58914) Hypothetical protein MJ1519 32 6.0
EX5A_BUCBP (Q89AB2) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 32 6.0
>YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergenic
region
Length = 723
Score = 70.1 bits (170), Expect = 3e-11
Identities = 122/483 (25%), Positives = 208/483 (42%), Gaps = 63/483 (13%)
Query: 473 FLYGFGGSGKTFVWNTLSAALRS-EGKIVLNV-ASSGIASLLLPGGRTAHSRFSIPITIH 530
F G G+GK+ + T+ L S GK + + AS+G+A++ + GG T H I I +
Sbjct: 251 FYTGSAGTGKSVILQTIIRQLSSLYGKESIAITASTGLAAVTI-GGSTLHKWSGIGIG-N 308
Query: 531 ESSTCNVRQGSHKAEML---QKASLIIWDEAPMLNKHCFEALDKTLNDIMKTQATFGHDK 587
++ V++ + ++L + ++I DE M++ + + L++ I K +D
Sbjct: 309 KTIDQLVKKIQSQKDLLAAWRYTKVLIIDEISMVDGNLLDKLEQIARRIRK------NDD 362
Query: 588 PFAGKVVVLGGDFRQILPVILKGSRSEIISSSANSSYLWKHC--KVMKLTTNMRLQQAGS 645
PF G +VL GDF Q+ PV K + + S +WK C K + LT R QQ
Sbjct: 363 PFGGIQLVLTGDFFQLPPVAKKDEHN--VVKFCFESEMWKRCIQKTILLTKVFR-QQDNK 419
Query: 646 SSSSLEIKEFADWLLQVGDGTIKPIDED----DSIIEIPTYLLVRESD----NPLLELVN 697
L + + + + TI+ ++ D D I Y RE + L L
Sbjct: 420 LIDILNAIRYGELTVDIAK-TIRNLNRDIDYADGIAPTELYATRREVELSNVKKLQSLPG 478
Query: 698 FAY-LNVVANL-ENHAYFEQRALLAPTLESVEEVNNFMM-------SMIPGEETKYLSYD 748
Y V N E + +L+ + +++E MM ++ G K L +
Sbjct: 479 DLYEFKAVDNAPERYQAILDSSLMVEKVVALKEDAQVMMLKNKPDVELVNGSLGKVLFFV 538
Query: 749 TPCRSDEDSEIDAEWFTSEFLNDLKCSGIPNHRIVLKVGVPIMLIQNIDQSAGLCNGTRL 808
T + EI + E + D+ R+V +V +L ++ + L
Sbjct: 539 TESLVVKMKEI-YKIVDDEVVMDM--------RLVSRVIGNPLLKESKEFRQDLNARPLA 589
Query: 809 IVSALTPYIIVATALSGSK---------TGKPVYIPRLSLT---PSDTGLPFK---FSRR 853
+ L I A +S K GK YI L + P D +P + R
Sbjct: 590 RLERLKILINYAVKISPHKEKFPYVRWTVGKNKYIHELMVPERFPID--IPRENVGLERT 647
Query: 854 QFPITVCFAMTINKSQGQSLSHVGLYLPRPVFTHGQLYVALSRVKSRKRLKILIVDDKGV 913
Q P+ +C+A++I+K+QGQ++ + + L R +F GQ+YVALSR + L++L D +
Sbjct: 648 QIPLMLCWALSIHKAQGQTIQRLKVDL-RRIFEAGQVYVALSRAVTMDTLQVLNFDPGKI 706
Query: 914 VSN 916
+N
Sbjct: 707 RTN 709
>PIF1_YEAST (P07271) DNA repair and recombination protein PIF1,
mitochondrial precursor
Length = 857
Score = 50.4 bits (119), Expect = 2e-05
Identities = 29/59 (49%), Positives = 41/59 (69%), Gaps = 1/59 (1%)
Query: 851 SRRQFPITVCFAMTINKSQGQSLSHVGLYLPRPVFTHGQLYVALSRVKSRKRLKILIVD 909
SR Q P+ + ++++I+KSQGQ+L V + L R VF GQ YVALSR SR+ L++L D
Sbjct: 690 SRVQLPLMLAWSLSIHKSQGQTLPKVKVDLRR-VFEKGQAYVALSRAVSREGLQVLNFD 747
Score = 43.5 bits (101), Expect = 0.003
Identities = 78/339 (23%), Positives = 130/339 (38%), Gaps = 63/339 (18%)
Query: 473 FLYGFGGSGKTFVWNTLSAALRS--EGKIVLNVASSGIASLLLPGGRTAHSRFSIPITIH 530
F G G+GK+ + + L+ + V AS+G+A+ + GG T HS F+ +
Sbjct: 255 FYTGSAGTGKSILLREMIKVLKGIYGRENVAVTASTGLAACNI-GGITIHS-FAGILGKG 312
Query: 531 ESSTCNVRQGSHKAEMLQK---ASLIIWDEAPMLNKHCFEALDKTLNDIMKTQATFGHDK 587
++ + G + L++ ++ DE ML+ + LD I K +
Sbjct: 313 DADKLYKKVGRRSRKHLRRWENIGALVVDEISMLDAELLDKLDFIARKIRKNH------Q 366
Query: 588 PFAGKVVVLGGDFRQILPVILKGSRSEIISSSANSSYLWKHCKVMKLTTNMRLQQAGSSS 647
PF G ++ GDF Q+ PV +R + A S WK M + +Q G
Sbjct: 367 PFGGIQLIFCGDFFQLPPVSKDPNRP---TKFAFESKAWKEGVKMTIMLQKVFRQRGD-- 421
Query: 648 SSLEIKEFADWLLQVGDGTIKPIDEDDSIIEIPTYLLVRESDNPLLELVNFAYLNVVANL 707
+F + L ++ G I +D++ E D +
Sbjct: 422 -----VKFIEMLNRMRLGNI----DDETEREFKKLSRPLPDDEII--------------- 457
Query: 708 ENHAYFEQRALLAPTLESVEEVNNFMMSMIPGEETKYLSYDTPCRSDEDSEIDAEWFTSE 767
A L T VE NN +S +PG+ + + D DE+ + E
Sbjct: 458 --------PAELYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALEDEELK---ERLLQN 506
Query: 768 FLNDLKCSGIPNHRIVLKVGVPIMLIQNIDQSAGLCNGT 806
FL + LKVG +M+++N+D A L NG+
Sbjct: 507 FL--------APKELHLKVGAQVMMVKNLD--ATLVNGS 535
>PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1,
mitochondrial precursor
Length = 805
Score = 50.4 bits (119), Expect = 2e-05
Identities = 77/340 (22%), Positives = 125/340 (36%), Gaps = 64/340 (18%)
Query: 473 FLYGFGGSGKTF----VWNTLSAALRSEGKIVLNVASSGIASLLLPGGRTAHSRFSIPIT 528
F G G+GK+ + L + R + V AS+G+A+ + GG T HS + +
Sbjct: 329 FFTGSAGTGKSVLLRKIIEVLKSKYRKQSDRVAVTASTGLAACNI-GGVTLHSFAGVGLA 387
Query: 529 IHESS--TCNVRQGSHKAEMLQKASLIIWDEAPMLNKHCFEALDKTLNDIMKTQATFGHD 586
+++ + ++I DE M++ + L++ I K
Sbjct: 388 RESVDLLVSKIKKNKKCVNRWLRTRVLIIDEVSMVDAELMDKLEEVARVIRKDS------ 441
Query: 587 KPFAGKVVVLGGDFRQILPVILKGSRSEIISSSANSSYLWKHCKVMKLTTNMRLQQAGSS 646
KPF G +VL GDF Q+ PV G S+ S WK + +Q
Sbjct: 442 KPFGGIQLVLTGDFFQLPPVPENGKESKFCFESQT----WKSALDFTIGLTHVFRQ---- 493
Query: 647 SSSLEIKEFADWLLQVGDGTIKPIDEDDSIIEIPTYLLVRESDNPLLELVNFAYLNVVAN 706
+ +EF L ++ G + D+S+ + E ++ LL F
Sbjct: 494 ----KDEEFVKMLNELRLGKL----SDESVRKFKVLNRTIEYEDGLLPTELF-------- 537
Query: 707 LENHAYFEQRALLAPTLESVEEVNNFMMSMIPGEETKYLSYDTPCRSDEDSEIDAEWFTS 766
PT VE N+ M I + + D+ D+
Sbjct: 538 --------------PTRYEVERSNDMRMQQINQNPVTFTAIDSGTVRDK----------- 572
Query: 767 EFLNDLKCSGIPNHRIVLKVGVPIMLIQNIDQSAGLCNGT 806
EF + L + +VLKV +MLI+NID L NG+
Sbjct: 573 EFRDRLLQGCMAPATLVLKVNAQVMLIKNIDDQ--LVNGS 610
Score = 49.3 bits (116), Expect = 5e-05
Identities = 26/56 (46%), Positives = 40/56 (71%), Gaps = 1/56 (1%)
Query: 851 SRRQFPITVCFAMTINKSQGQSLSHVGLYLPRPVFTHGQLYVALSRVKSRKRLKIL 906
SR Q P+ + +A++I+K+QGQ+L V + L R VF GQ YVALSR +++ L++L
Sbjct: 707 SRSQIPLILAYAISIHKAQGQTLDRVKVDLGR-VFEKGQAYVALSRATTQEGLQVL 761
>EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5) (Exodeoxyribonuclease V 67 kDa polypeptide)
Length = 608
Score = 36.6 bits (83), Expect = 0.32
Identities = 19/46 (41%), Positives = 26/46 (56%), Gaps = 3/46 (6%)
Query: 861 FAMTINKSQGQSLSHVGLYLP---RPVFTHGQLYVALSRVKSRKRL 903
+AMT++KSQG H L LP PV T +Y A++R + R L
Sbjct: 534 WAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRRLSL 579
>HELI_EHV1B (P28934) Probable helicase
Length = 881
Score = 35.0 bits (79), Expect = 0.92
Identities = 19/44 (43%), Positives = 26/44 (58%)
Query: 862 AMTINKSQGQSLSHVGLYLPRPVFTHGQLYVALSRVKSRKRLKI 905
AMTI +SQG SL V + PR +YVA+SR S + L++
Sbjct: 806 AMTIARSQGLSLDKVAICFPRNNLRINSVYVAMSRTVSSRFLRM 849
>PKSJ_BACSU (P40806) Putative polyketide synthase pksJ (PKS)
Length = 5045
Score = 34.7 bits (78), Expect = 1.2
Identities = 25/88 (28%), Positives = 40/88 (45%), Gaps = 12/88 (13%)
Query: 755 EDSEIDAEWFTSEFLNDLKCSGIPNHRIVLKVGVPIMLIQNIDQSAGLCNGTRLIVSALT 814
EDSE+ TSE +N L +G+ +L Q+ D+ A + +++ +T
Sbjct: 1250 EDSEVFITLTTSELVNTLSWNGV----------TTALLDQDWDEIAQTASDRKVLTRTVT 1299
Query: 815 PYIIVATALSGSKTGKP--VYIPRLSLT 840
P + + TGKP V IP +LT
Sbjct: 1300 PENLAYVIYTSGSTGKPKGVMIPHKALT 1327
>THDE_MOUSE (Q8K093) Thyrotropin-releasing hormone degrading
ectoenzyme (EC 3.4.19.6) (TRH-degrading ectoenzyme)
(TRH-DE) (TRH-specific aminopeptidase) (Thyroliberinase)
(Pyroglutamyl-peptidase II) (PAP-II)
Length = 1025
Score = 34.3 bits (77), Expect = 1.6
Identities = 14/38 (36%), Positives = 24/38 (62%)
Query: 463 LFRPITEDFFFLYGFGGSGKTFVWNTLSAALRSEGKIV 500
+F+ +D+ ++ +G + + +WNTLS ALR GK V
Sbjct: 546 VFQRGLQDYLTIHKYGNAARNDLWNTLSEALRRNGKYV 583
>TA4B_HUMAN (Q92750) Transcription initiation factor TFIID 105 kDa
subunit (TAFII-105) (TAFII105) (Fragment)
Length = 801
Score = 33.5 bits (75), Expect = 2.7
Identities = 49/219 (22%), Positives = 97/219 (43%), Gaps = 30/219 (13%)
Query: 646 SSSSLEIKEFA----DWLLQVGDGTIKPIDEDDSI--IEIPTYLLVRESDNPLLELVNFA 699
+SSS IK+ A L Q+G + + + S+ + +PT ++ PL +
Sbjct: 98 NSSSQLIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLK 157
Query: 700 YLNVVA--------NLENHAYFEQRALLAPT-LESVEEVNNFMMSMIP----GEETKYLS 746
++ A NL+ + L+PT LE+V++ NF+ +I G ++ +
Sbjct: 158 PSSLGASSTPSNEPNLKAENSAAVQINLSPTMLENVKKCKNFLAMLIKLACSGSQSPEMG 217
Query: 747 YDTPCRSDE--DSEIDAEWFTSEFLNDLKCSGIPNHRIVLKVGVPIM--LIQNIDQSAGL 802
+ ++ D++I+AE FT + +LK S P+ LK V + L+ N
Sbjct: 218 QNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQ 277
Query: 803 C---NGTRLIVSALTPYI----IVATALSGSKTGKPVYI 834
C + ++++ T + +V T +S S++ K + +
Sbjct: 278 CVQQTSSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIV 316
>THDE_RAT (Q10836) Thyrotropin-releasing hormone degrading
ectoenzyme (EC 3.4.19.6) (TRH-degrading ectoenzyme)
(TRH-DE) (TRH-specific aminopeptidase) (Thyroliberinase)
(Pyroglutamyl-peptidase II) (PAP-II)
Length = 1025
Score = 33.1 bits (74), Expect = 3.5
Identities = 13/38 (34%), Positives = 24/38 (62%)
Query: 463 LFRPITEDFFFLYGFGGSGKTFVWNTLSAALRSEGKIV 500
+F+ +D+ ++ +G + + +WNTLS AL+ GK V
Sbjct: 546 VFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYV 583
>THDE_HUMAN (Q9UKU6) Thyrotropin-releasing hormone degrading
ectoenzyme (EC 3.4.19.6) (TRH-degrading ectoenzyme)
(TRH-DE) (TRH-specific aminopeptidase) (Thyroliberinase)
(Pyroglutamyl-peptidase II) (PAP-II)
Length = 1024
Score = 33.1 bits (74), Expect = 3.5
Identities = 13/38 (34%), Positives = 24/38 (62%)
Query: 463 LFRPITEDFFFLYGFGGSGKTFVWNTLSAALRSEGKIV 500
+F+ +D+ ++ +G + + +WNTLS AL+ GK V
Sbjct: 545 VFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYV 582
>KITH_EHV4 (P24425) Thymidine kinase (EC 2.7.1.21)
Length = 352
Score = 32.7 bits (73), Expect = 4.6
Identities = 26/85 (30%), Positives = 37/85 (42%), Gaps = 8/85 (9%)
Query: 518 TAH--SRFSIPITIHESSTCNVRQGSHKAEMLQKASLIIWDEAPMLNKHCFEALDKTLND 575
TAH SRF+ P I TC + G+ Q +++D P+ + CF A L D
Sbjct: 98 TAHYQSRFTTPYLILHDHTCTLFGGNSLQRGTQPDLTLVFDRHPVASTVCFPAARYLLGD 157
Query: 576 -----IMKTQATFGHDKPFAGKVVV 595
+M AT + P G +VV
Sbjct: 158 MSMCALMAMVATLPRE-PQGGNIVV 181
>YF19_METJA (Q58914) Hypothetical protein MJ1519
Length = 1175
Score = 32.3 bits (72), Expect = 6.0
Identities = 18/53 (33%), Positives = 33/53 (61%), Gaps = 5/53 (9%)
Query: 861 FAMTINKSQGQSLSHVGLYLPRPV---FTHGQLYVALSRVKSRKRLKILIVDD 910
+A+TI+KSQG +V L +P+ + + LY A++R K KRL +++ ++
Sbjct: 961 YAITIHKSQGSGFENVILIIPKGLNKFVSKEMLYTAITRAK--KRLYVIVEEE 1011
>EX5A_BUCBP (Q89AB2) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5)
Length = 618
Score = 32.3 bits (72), Expect = 6.0
Identities = 21/65 (32%), Positives = 36/65 (55%), Gaps = 6/65 (9%)
Query: 861 FAMTINKSQGQSLSHVGLYLP---RPVFTHGQLYVALSRVKSRKRLKILIVDDKGVVSNC 917
+ MT++KSQG S V L LP + T +Y A++R S+K+L I D+ + +
Sbjct: 544 WTMTVHKSQGSEFSEVVLILPTIMTSILTKELIYTAVTR--SKKKLTI-YSDENIFIKSL 600
Query: 918 TRNVV 922
+N++
Sbjct: 601 KKNII 605
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.344 0.151 0.484
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,384,140
Number of Sequences: 164201
Number of extensions: 3488117
Number of successful extensions: 15745
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 15724
Number of HSP's gapped (non-prelim): 24
length of query: 930
length of database: 59,974,054
effective HSP length: 120
effective length of query: 810
effective length of database: 40,269,934
effective search space: 32618646540
effective search space used: 32618646540
T: 11
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 71 (32.0 bits)
Lotus: description of TM0320b.5