BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0318.14
(58 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAK_MOUSE (Q9Z1W9) STE20/SPS1-related proline-alanine rich prot... 31 0.53
HOB1_SCHPO (O74352) Protein hob1 (Homolog of Bin1) 31 0.53
MAP2_MOUSE (P20357) Microtubule-associated protein 2 (MAP 2) 30 1.2
CH60_EUGGR (Q39727) Chaperonin CPN60, mitochondrial precursor (H... 30 1.6
TP15_HUMAN (Q9UPQ8) Transmembrane protein 15 (UNQ2422/PRO4980) 29 2.7
MNTH_SALTY (Q9RPF4) Manganese transport protein mntH 29 2.7
MNTH_SALTI (Q8Z4X5) Manganese transport protein mntH 29 2.7
ODP2_ALCEU (Q59098) Dihydrolipoyllysine-residue acetyltransferas... 28 3.5
MNTH_ECOLI (P77145) Manganese transport protein mntH 28 3.5
MAP2_HUMAN (P11137) Microtubule-associated protein 2 (MAP 2) (MA... 28 3.5
PUCK_BACSU (O32140) Uric acid permease pucK 28 4.5
NL13_PARJU (Q40905) Probable nonspecific lipid-transfer protein ... 28 4.5
MTRC_METKA (O32865) Tetrahydromethanopterin S-methyltransferase ... 28 4.5
EXBB_PSEPU (Q05605) Biopolymer transport exbB protein 28 4.5
VTER_EBV (P03219) Probable DNA packaging protein 28 5.9
RLA1_LEIPE (O46313) 60S acidic ribosomal protein P1 28 5.9
NL21_PARJU (P55958) Probable nonspecific lipid-transfer protein ... 28 5.9
FXD2_MOUSE (O35392) Forkhead box protein D2 (Mesoderm/mesenchyme... 28 5.9
Z179_RAT (O70418) Zinc finger protein 179 (Brain finger protein) 27 7.7
Z179_HUMAN (Q9ULX5) Zinc finger protein 179 (Brain finger protein) 27 7.7
>SPAK_MOUSE (Q9Z1W9) STE20/SPS1-related proline-alanine rich
protein kinase (EC 2.7.1.37) (Ste-20 related kinase)
(Serine/threonine-protein kinase 39)
Length = 556
Score = 31.2 bits (69), Expect = 0.53
Identities = 14/28 (50%), Positives = 17/28 (60%)
Query: 12 IVAASMSAALATNEVPAPAPGPASGASA 39
+ AA+ +A A PAPAP PA ASA
Sbjct: 20 VTAAAATAPAAATSAPAPAPAPAPAASA 47
Score = 27.7 bits (60), Expect = 5.9
Identities = 13/29 (44%), Positives = 17/29 (57%)
Query: 10 VLIVAASMSAALATNEVPAPAPGPASGAS 38
V AA+ AA + PAPAP PA+ A+
Sbjct: 20 VTAAAATAPAAATSAPAPAPAPAPAASAA 48
>HOB1_SCHPO (O74352) Protein hob1 (Homolog of Bin1)
Length = 466
Score = 31.2 bits (69), Expect = 0.53
Identities = 23/60 (38%), Positives = 28/60 (46%), Gaps = 5/60 (8%)
Query: 1 MDMKKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLV-----GASVLSFFA 55
M V VAA ++AA A VP PAP PA+ +A V +L A LSF A
Sbjct: 371 MPQPAAAAVTTPVAAPVAAAAAAVPVPPPAPAPAAAPAAEHVVALYDYAAQAAGDLSFHA 430
>MAP2_MOUSE (P20357) Microtubule-associated protein 2 (MAP 2)
Length = 1828
Score = 30.0 bits (66), Expect = 1.2
Identities = 16/40 (40%), Positives = 22/40 (55%)
Query: 14 AASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSF 53
A ++A N P+P P PAS +ATV L+ AS + F
Sbjct: 122 ALPLAAEETANLPPSPPPSPASEQTATVEEDLLTASKMEF 161
>CH60_EUGGR (Q39727) Chaperonin CPN60, mitochondrial precursor (HSP
60)
Length = 569
Score = 29.6 bits (65), Expect = 1.6
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Query: 1 MDMKKVTCVVLIVAASMSAALATNEVPA---PAPGPASGASATVVGSLVG 47
+D KV L+ AAS++ + T E PAP PA+G +G + G
Sbjct: 512 IDPAKVVKTALVDAASVAGLMITTEAAITDIPAPAPAAGGGMDGMGGMGG 561
>TP15_HUMAN (Q9UPQ8) Transmembrane protein 15 (UNQ2422/PRO4980)
Length = 538
Score = 28.9 bits (63), Expect = 2.7
Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Query: 23 TNEVPAPAPGPASGASATVV--GSLVGASVLSFFA 55
T E P+PAPGP + S +V+ ++V A VLS A
Sbjct: 2 TRECPSPAPGPGAPLSGSVLAEAAVVFAVVLSIHA 36
>MNTH_SALTY (Q9RPF4) Manganese transport protein mntH
Length = 413
Score = 28.9 bits (63), Expect = 2.7
Identities = 13/25 (52%), Positives = 17/25 (68%)
Query: 34 ASGASATVVGSLVGASVLSFFALFH 58
A+G S+TVVG+L G V+ F FH
Sbjct: 297 AAGLSSTVVGTLAGQVVMQGFVRFH 321
>MNTH_SALTI (Q8Z4X5) Manganese transport protein mntH
Length = 413
Score = 28.9 bits (63), Expect = 2.7
Identities = 13/25 (52%), Positives = 17/25 (68%)
Query: 34 ASGASATVVGSLVGASVLSFFALFH 58
A+G S+TVVG+L G V+ F FH
Sbjct: 297 AAGLSSTVVGTLAGQVVMQGFVRFH 321
>ODP2_ALCEU (Q59098) Dihydrolipoyllysine-residue acetyltransferase
component of pyruvate dehydrogenase complex (EC
2.3.1.12) (E2) (Dihydrolipoamide acetyltransferase
component of pyruvate dehydrogenase complex)
Length = 553
Score = 28.5 bits (62), Expect = 3.5
Identities = 13/27 (48%), Positives = 16/27 (59%)
Query: 14 AASMSAALATNEVPAPAPGPASGASAT 40
AA+ +A PAPAP PA+ AS T
Sbjct: 205 AAAAAAPAPAASAPAPAPAPAAAASGT 231
>MNTH_ECOLI (P77145) Manganese transport protein mntH
Length = 412
Score = 28.5 bits (62), Expect = 3.5
Identities = 13/25 (52%), Positives = 17/25 (68%)
Query: 34 ASGASATVVGSLVGASVLSFFALFH 58
A+G S+TVVG+L G V+ F FH
Sbjct: 297 AAGLSSTVVGTLAGQVVMQGFIRFH 321
>MAP2_HUMAN (P11137) Microtubule-associated protein 2 (MAP 2)
(MAP-2)
Length = 1827
Score = 28.5 bits (62), Expect = 3.5
Identities = 15/40 (37%), Positives = 21/40 (52%)
Query: 14 AASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSF 53
A ++A N P+P P PAS + TV L+ AS + F
Sbjct: 122 ALPLAAEETANLPPSPPPSPASEQTVTVEEDLLTASKMEF 161
>PUCK_BACSU (O32140) Uric acid permease pucK
Length = 430
Score = 28.1 bits (61), Expect = 4.5
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 5/50 (10%)
Query: 10 VLIVAASMSAALATNEVPA---PAPGPAS--GASATVVGSLVGASVLSFF 54
+LI+A+S+S L VPA G AS S V+GSL ++ +FF
Sbjct: 369 LLIIASSVSLGLGATTVPALFSSLSGAASVLAGSGIVIGSLTAIALHAFF 418
>NL13_PARJU (Q40905) Probable nonspecific lipid-transfer protein 1
precursor (LTP) (Major pollen allergen Par j 1.0201)
(Par j I) (P1 protein)
Length = 138
Score = 28.1 bits (61), Expect = 4.5
Identities = 16/38 (42%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Query: 9 VVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLV 46
+V+IVAA + + + PAPAPG + TVVG+L+
Sbjct: 12 LVVIVAAVLVWTSSASVAPAPAPG-SEETCGTVVGALM 48
>MTRC_METKA (O32865) Tetrahydromethanopterin S-methyltransferase
subunit C (EC 2.1.1.86)
(N5-methyltetrahydromethanopterin--coenzyme M
methyltransferase subunit C)
Length = 270
Score = 28.1 bits (61), Expect = 4.5
Identities = 16/35 (45%), Positives = 21/35 (59%)
Query: 13 VAASMSAALATNEVPAPAPGPASGASATVVGSLVG 47
VAA ++AAL+ N VPA A VVG+L+G
Sbjct: 93 VAAILAAALSGNTVPAWAAAIIGTVIGAVVGALLG 127
>EXBB_PSEPU (Q05605) Biopolymer transport exbB protein
Length = 329
Score = 28.1 bits (61), Expect = 4.5
Identities = 16/36 (44%), Positives = 18/36 (49%)
Query: 15 ASMSAALATNEVPAPAPGPASGASATVVGSLVGASV 50
AS AA A PA AP PA+ SA V + A V
Sbjct: 41 ASTPAAAAAPATPAAAPAPAADGSAPVADAPAAAPV 76
>VTER_EBV (P03219) Probable DNA packaging protein
Length = 690
Score = 27.7 bits (60), Expect = 5.9
Identities = 14/43 (32%), Positives = 23/43 (52%)
Query: 7 TCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLVGAS 49
TCVV +V++ ++ EV G +S SA + S++G S
Sbjct: 531 TCVVALVSSIVTLHPHLEEVKVAVEGNSSQDSAVAIASIIGES 573
>RLA1_LEIPE (O46313) 60S acidic ribosomal protein P1
Length = 107
Score = 27.7 bits (60), Expect = 5.9
Identities = 14/34 (41%), Positives = 20/34 (58%)
Query: 3 MKKVTCVVLIVAASMSAALATNEVPAPAPGPASG 36
++K + L+ AA+ A AT+ APA G ASG
Sbjct: 50 LEKKSVEALMAAAATQAPTATSAAAAPAAGEASG 83
>NL21_PARJU (P55958) Probable nonspecific lipid-transfer protein 2
precursor (LTP 2) (Major pollen allergen Par j 2.0101)
(Par j II) (P2 protein)
Length = 133
Score = 27.7 bits (60), Expect = 5.9
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Query: 3 MKKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLV 46
M+ V+ L+V A+ A ++ E PAPAP P A VV ++
Sbjct: 1 MRTVSMAALVVIAAALAWTSSAE-PAPAPAPGEEACGKVVQDIM 43
>FXD2_MOUSE (O35392) Forkhead box protein D2 (Mesoderm/mesenchyme
forkhead 2) (MF-2)
Length = 492
Score = 27.7 bits (60), Expect = 5.9
Identities = 15/36 (41%), Positives = 22/36 (60%), Gaps = 2/36 (5%)
Query: 15 ASMSAALATNEVPAPAPGPASGASATVVGSLVGASV 50
AS+ A+ A+ PAPAP P SG S + + +GA +
Sbjct: 324 ASVFASAAS--APAPAPAPGSGPSPAGLPAFLGAEL 357
>Z179_RAT (O70418) Zinc finger protein 179 (Brain finger protein)
Length = 631
Score = 27.3 bits (59), Expect = 7.7
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Query: 12 IVAASMSAALATNEVPAPAPGPASGAS-ATVVGSLVGASV 50
+V A M+AA E A G A GA+ A VVG VGA +
Sbjct: 565 VVGAGMAAAALAAEAGMVAAGAAVGATGAAVVGGGVGAGL 604
>Z179_HUMAN (Q9ULX5) Zinc finger protein 179 (Brain finger protein)
Length = 632
Score = 27.3 bits (59), Expect = 7.7
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Query: 12 IVAASMSAALATNEVPAPAPGPASGAS-ATVVGSLVGASV 50
+V A M+AA E A G A GA+ A VVG VGA +
Sbjct: 566 VVGAGMAAAALAAEAGMVAAGAAVGATGAAVVGGGVGAGL 605
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.128 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,639,776
Number of Sequences: 164201
Number of extensions: 153385
Number of successful extensions: 1705
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1658
Number of HSP's gapped (non-prelim): 41
length of query: 58
length of database: 59,974,054
effective HSP length: 34
effective length of query: 24
effective length of database: 54,391,220
effective search space: 1305389280
effective search space used: 1305389280
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0318.14