BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.16
(185 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UGP5_ARATH (Q9C7F7) Uncharacterized GPI-anchored protein At1g279... 50 2e-06
NLT1_TOBAC (Q42952) Nonspecific lipid-transfer protein 1 precurs... 46 4e-05
NLT2_LYCES (P27056) Nonspecific lipid-transfer protein 2 precurs... 44 2e-04
MC1_PINRA (O24493) Male-cone protein 1 precursor (PRMC1) 44 2e-04
NLTC_RICCO (P10975) Nonspecific lipid-transfer protein C, cotyle... 44 3e-04
NLTD_RICCO (Q43119) Nonspecific lipid-transfer protein D, cotyle... 42 8e-04
NLT2_ARATH (Q9S7I3) Nonspecific lipid-transfer protein 2 precurs... 42 0.001
NLT2_SORBI (Q43194) Nonspecific lipid-transfer protein 2 precurs... 41 0.001
NLTA_RICCO (P10973) Nonspecific lipid-transfer protein A (NS-LTP... 41 0.002
NLTA_BRAOL (Q42641) Nonspecific lipid-transfer protein A precurs... 41 0.002
NLT2_TOBAC (Q03461) Nonspecific lipid-transfer protein 2 precurs... 41 0.002
NLT1_HORVU (P07597) Nonspecific lipid-transfer protein 1 precurs... 40 0.002
NL41_HORVU (Q43767) Nonspecific lipid-transfer protein 4.1 precu... 40 0.002
NLTP_PYRCO (Q9M5X6) Nonspecific lipid-transfer protein precursor... 40 0.004
NLTP_CICAR (O23758) Nonspecific lipid-transfer protein precursor... 39 0.005
NLTP_HELAN (Q39950) Nonspecific lipid-transfer protein precursor... 39 0.007
NLTP_BETVU (Q43748) Nonspecific lipid-transfer protein precursor... 39 0.007
NLTP_GERHY (Q39794) Nonspecific lipid-transfer protein precursor... 39 0.009
NLTP_MAIZE (P19656) Nonspecific lipid-transfer protein precursor... 38 0.012
NL43_HORVU (Q42842) Nonspecific lipid-transfer protein 4.3 precu... 38 0.012
>UGP5_ARATH (Q9C7F7) Uncharacterized GPI-anchored protein At1g27950
precursor
Length = 193
Score = 50.4 bits (119), Expect = 2e-06
Identities = 32/113 (28%), Positives = 56/113 (49%), Gaps = 9/113 (7%)
Query: 7 LILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGL 66
L+L + I A A +A + A + +C+ K+T CL F T +T+ P +CC +
Sbjct: 8 LVLVTMTIVA-SIAAAAPAAPGGALADECNQDFQKVTLCLDFATGKATI--PSKKCCDAV 64
Query: 67 KSVLKTAPSCLCEAFKSSSQFGVVL-----NVTKALALPSACKVSASNSVTNC 114
+ + + P CLC + + G L K + LP++C++ + S+TNC
Sbjct: 65 EDIKERDPKCLCFVIQQAKTGGQALKDLGVQEDKLIQLPTSCQLH-NASITNC 116
>NLT1_TOBAC (Q42952) Nonspecific lipid-transfer protein 1 precursor
(LTP 1)
Length = 114
Score = 46.2 bits (108), Expect = 4e-05
Identities = 30/104 (28%), Positives = 49/104 (46%), Gaps = 16/104 (15%)
Query: 8 ILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLK 67
I C V +C V A A + C + + CL+++ N T P G+CC G+K
Sbjct: 7 IACFVVLCMVVAAPCAEA-------ITCGQVTSNLAPCLAYLRN----TGPLGRCCGGVK 55
Query: 68 SVLKTAPS-----CLCEAFKSSSQFGVVLNVTKALALPSACKVS 106
+++ +A + C KS++ +N+ KA LPS C V+
Sbjct: 56 ALVNSARTTEDRQIACTCLKSAAGAISGINLGKAAGLPSTCGVN 99
>NLT2_LYCES (P27056) Nonspecific lipid-transfer protein 2 precursor
(LTP 2)
Length = 114
Score = 44.3 bits (103), Expect = 2e-04
Identities = 30/107 (28%), Positives = 48/107 (44%), Gaps = 16/107 (14%)
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
VS I C V +C V A A + C + + CL ++ P G CC
Sbjct: 4 VSKIACFVLLCMVVVAPHAEA-------LTCGQVTAGLAPCLPYLQGRG----PLGGCCG 52
Query: 65 GLKSVLKTAPSCL-----CEAFKSSSQFGVVLNVTKALALPSACKVS 106
G+K++L +A + C KS++ +++ KA +PS CKV+
Sbjct: 53 GVKNLLGSAKTTADRKTACTCLKSAANAIKGIDLNKAAGIPSVCKVN 99
>MC1_PINRA (O24493) Male-cone protein 1 precursor (PRMC1)
Length = 92
Score = 43.9 bits (102), Expect = 2e-04
Identities = 25/80 (31%), Positives = 37/80 (46%), Gaps = 15/80 (18%)
Query: 28 APAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSSQF 87
A + DCSN + K+ C S V S KP +CC LK T+ SC+C+
Sbjct: 21 AAVQAEDCSNAMDKLAPCTSAVGLSSNGVKPSSECCDALKG---TSTSCVCK-------- 69
Query: 88 GVVLNVTKALALPSACKVSA 107
+V ++LP+ C + A
Sbjct: 70 ----SVRAVISLPAKCNLPA 85
>NLTC_RICCO (P10975) Nonspecific lipid-transfer protein C,
cotyledon-specific isoform precursor (NS-LTP C)
(Phospholipid transfer protein) (PLTP)
Length = 116
Score = 43.5 bits (101), Expect = 3e-04
Identities = 29/101 (28%), Positives = 48/101 (46%), Gaps = 12/101 (11%)
Query: 23 ASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS-----CL 77
A++N A P CS + +K C+ F T + KP CC+GL+ + +T + +
Sbjct: 19 ANTNEAAVP---CSTVDMKAAACVGFATGKDS--KPSQACCTGLQQLAQTVKTVDDKKAI 73
Query: 78 CEAFKSSSQFGVVLNVTKALALPSACKVSASNSV-TNCGCE 117
C K+SS+ + + +P+AC + V TN CE
Sbjct: 74 CRCLKASSK-SLGIKDQFLSKIPAACNIKVGFPVSTNTNCE 113
>NLTD_RICCO (Q43119) Nonspecific lipid-transfer protein D,
cotyledon-specific isoform precursor (NS-LTP D)
Length = 116
Score = 42.0 bits (97), Expect = 8e-04
Identities = 25/96 (26%), Positives = 44/96 (45%), Gaps = 11/96 (11%)
Query: 27 NAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS-----CLCEAF 81
N + CS + +K C+ F T + KP CC+GL+ + +T S +C
Sbjct: 20 NVSEAAVPCSTVDMKAAACVGFATGKDS--KPSSACCTGLQQLAQTVKSVDDKKAICRCL 77
Query: 82 KSSSQFGVVLNVTKALALPSACKVSAS---NSVTNC 114
K+SS+ + + +P+AC + ++ TNC
Sbjct: 78 KASSK-SLGIKDQFLSKIPAACNIKVGFPVSTATNC 112
>NLT2_ARATH (Q9S7I3) Nonspecific lipid-transfer protein 2 precursor
(LTP 2)
Length = 118
Score = 41.6 bits (96), Expect = 0.001
Identities = 35/119 (29%), Positives = 59/119 (49%), Gaps = 17/119 (14%)
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
V + C+V C + A ++N + C + + C++++T + +T QG CC+
Sbjct: 4 VMKLACMVLACMI-VAGPITANALMS----CGTVNGNLAGCIAYLTRGAPLT--QG-CCN 55
Query: 65 G---LKSVLKTAPSC--LCEAFKSSSQ-FGVVLNVTKALALPSACKVSASNSV---TNC 114
G LK++ T P C +S+++ G LN +A LPSACKV+ + TNC
Sbjct: 56 GVTNLKNMASTTPDRQQACRCLQSAAKAVGPGLNTARAAGLPSACKVNIPYKISASTNC 114
>NLT2_SORBI (Q43194) Nonspecific lipid-transfer protein 2 precursor
(LTP 2)
Length = 122
Score = 41.2 bits (95), Expect = 0.001
Identities = 30/114 (26%), Positives = 53/114 (46%), Gaps = 11/114 (9%)
Query: 4 KVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCC 63
K+++ + VVA A + +S A C + + CLS+ + P CC
Sbjct: 6 KLAVAIAVVAAAAAVVLAATTSEAA----VTCGQVSSAIGPCLSYARGQGS--GPSAGCC 59
Query: 64 SGLKSVLKTAPS-----CLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVT 112
SG++S+ A + C K++++ LNV KA ++PS C VS +++
Sbjct: 60 SGVRSLNSAARTTADRRAACNCLKNAARGIRGLNVGKAASIPSKCGVSIPYTIS 113
>NLTA_RICCO (P10973) Nonspecific lipid-transfer protein A (NS-LTP A)
(Phospholipid transfer protein) (PLTP)
Length = 92
Score = 40.8 bits (94), Expect = 0.002
Identities = 23/89 (25%), Positives = 40/89 (44%), Gaps = 10/89 (11%)
Query: 34 DCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS-----CLCEAFKSSSQFG 88
DC + + C+ F+T V P CC+G++++ AP+ CE K+++
Sbjct: 2 DCGQVNSSLASCIPFLTGG--VASPSASCCAGVQNLKTLAPTSADRRAACECIKAAAARF 59
Query: 89 VVLNVTKALALPSACKVSAS---NSVTNC 114
+ A +LP C V + + TNC
Sbjct: 60 PTIKQDAASSLPKKCGVDINIPISKTTNC 88
>NLTA_BRAOL (Q42641) Nonspecific lipid-transfer protein A precursor
(LTP A) (Wax-associated protein 9A)
Length = 118
Score = 40.8 bits (94), Expect = 0.002
Identities = 32/119 (26%), Positives = 54/119 (44%), Gaps = 15/119 (12%)
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
V + C+V C + A ++N A C + + C+ ++T T+ G CC+
Sbjct: 4 VMKLACLVLACMI-VAGPITANRA----LTCGTVNSNVAPCIGYITQGGTLP---GACCT 55
Query: 65 G---LKSVLKTAPSC--LCEAFKSSSQ-FGVVLNVTKALALPSACKVSASNSV-TNCGC 116
G L S+ +T P C +++++ G LN +A +P AC VS + TN C
Sbjct: 56 GVSKLNSMARTTPDRQQACRCLETAARALGPNLNAGRAAGIPKACGVSVPFPISTNTNC 114
>NLT2_TOBAC (Q03461) Nonspecific lipid-transfer protein 2 precursor
(LTP 2)
Length = 114
Score = 40.8 bits (94), Expect = 0.002
Identities = 30/107 (28%), Positives = 44/107 (41%), Gaps = 16/107 (14%)
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
V I C V +C V A A + C + + CL ++ P G CC
Sbjct: 4 VGKIACFVVLCMVVVAPHAEA-------LSCGQVQSGLAPCLPYLQGRG----PLGSCCG 52
Query: 65 GLKSVLKTAPS-----CLCEAFKSSSQFGVVLNVTKALALPSACKVS 106
G+K +L A S C KS++ +++ KA LP AC V+
Sbjct: 53 GVKGLLGAAKSLSDRKTACTCLKSAANAIKGIDMGKAAGLPGACGVN 99
>NLT1_HORVU (P07597) Nonspecific lipid-transfer protein 1 precursor
(LTP 1) (Probable amylase/protease inhibitor)
Length = 117
Score = 40.4 bits (93), Expect = 0.002
Identities = 23/88 (26%), Positives = 43/88 (48%), Gaps = 8/88 (9%)
Query: 30 APSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS-----CLCEAFKSS 84
A + +C + KM CL++V P G+CC+G++ + A S +C K
Sbjct: 24 AVALNCGQVDSKMKPCLTYVQGGPG---PSGECCNGVRDLHNQAQSSGDRQTVCNCLKGI 80
Query: 85 SQFGVVLNVTKALALPSACKVSASNSVT 112
++ LN+ A ++PS C V+ +++
Sbjct: 81 ARGIHNLNLNNAASIPSKCNVNVPYTIS 108
>NL41_HORVU (Q43767) Nonspecific lipid-transfer protein 4.1
precursor (LTP 4.1) (CW21) (CW-21)
Length = 115
Score = 40.4 bits (93), Expect = 0.002
Identities = 31/116 (26%), Positives = 51/116 (43%), Gaps = 20/116 (17%)
Query: 2 ASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQ 61
AS++ L+ V A+ V A + C + ++ C+S+ + KP
Sbjct: 6 ASQLVLVALVAAMLLV----------AADAAISCGQVSSALSPCISYARGNGA--KPPAA 53
Query: 62 CCSGLKSVLKTAPS-----CLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVT 112
CCSG+K + A S C+ KS++ LN KA +PS C VS +++
Sbjct: 54 CCSGVKRLAGAAQSTADKQAACKCIKSAAG---GLNAGKAAGIPSMCGVSVPYAIS 106
>NLTP_PYRCO (Q9M5X6) Nonspecific lipid-transfer protein precursor
(LTP) (Allergen Pyr c 3)
Length = 115
Score = 39.7 bits (91), Expect = 0.004
Identities = 36/124 (29%), Positives = 56/124 (45%), Gaps = 26/124 (20%)
Query: 2 ASKVSLILCVVAIC-AVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
+S V + VVA+C AV AH+ + CS + + C+++V + V
Sbjct: 3 SSAVIKLALVVALCMAVSVAHAIT----------CSQVSANLAPCINYVRSGGAVPP--- 49
Query: 61 QCCSGLKSV---LKTAP----SCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSV-- 111
CC+G+K++ KT P +C C + S GV N A +LP C V+ +
Sbjct: 50 ACCNGIKTINGLAKTTPDRQAACNCLKNLAGSVSGV--NPGNAESLPGKCGVNVPYKIST 107
Query: 112 -TNC 114
TNC
Sbjct: 108 STNC 111
>NLTP_CICAR (O23758) Nonspecific lipid-transfer protein precursor
(LTP)
Length = 116
Score = 39.3 bits (90), Expect = 0.005
Identities = 32/115 (27%), Positives = 44/115 (37%), Gaps = 17/115 (14%)
Query: 8 ILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSG-- 65
++CV I + A A S + C + + CL ++ P QCC G
Sbjct: 6 VVCVALIMCIVIAPMAES------AITCGRVDTALAPCLGYLQGGPG---PSAQCCGGVR 56
Query: 66 -LKSVLKTAPS--CLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSV---TNC 114
L S T P C KS++ LN A ALP C V+ + TNC
Sbjct: 57 NLNSAAVTTPDRQAACNCLKSAAGSISRLNANNAAALPGKCVVNIPYKISTSTNC 111
>NLTP_HELAN (Q39950) Nonspecific lipid-transfer protein precursor
(LTP) (NsLTP) (SDI-9)
Length = 116
Score = 38.9 bits (89), Expect = 0.007
Identities = 32/112 (28%), Positives = 44/112 (38%), Gaps = 17/112 (15%)
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSA-DCSNLILKMTDCLSFVTNDSTVTKPQ 59
MA ++LC C V AP A C + + C+S++T V
Sbjct: 1 MAKMAMMVLCAGVTCMVV--------GAPYTEALSCGQVSSSLAPCISYLTKGGAVPP-- 50
Query: 60 GQCCSGLKSV---LKTAP--SCLCEAFKSSSQFGVVLNVTKALALPSACKVS 106
CCSG+KS+ KT P C KS+ +N A + P C VS
Sbjct: 51 -ACCSGVKSLNSAAKTTPDRQAACGCLKSAYNSISGVNAGNAASFPGKCGVS 101
>NLTP_BETVU (Q43748) Nonspecific lipid-transfer protein precursor
(LTP)
Length = 117
Score = 38.9 bits (89), Expect = 0.007
Identities = 26/93 (27%), Positives = 40/93 (42%), Gaps = 11/93 (11%)
Query: 30 APSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS-----CLCEAFKSS 84
A + C + K+ C+ ++ P CC G+KS+ A S C KS+
Sbjct: 24 AEAITCGLVASKLAPCIGYLQG---APGPSAACCGGIKSLNSAAASPADRKTACTCLKSA 80
Query: 85 SQFGVVLNVTKALALPSACKVSASNSV---TNC 114
+ +N KA +LP C VS ++ TNC
Sbjct: 81 ATSIKGINYGKAASLPRQCGVSVPYAISPNTNC 113
>NLTP_GERHY (Q39794) Nonspecific lipid-transfer protein precursor
(LTP)
Length = 116
Score = 38.5 bits (88), Expect = 0.009
Identities = 34/122 (27%), Positives = 47/122 (37%), Gaps = 16/122 (13%)
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
MAS V +LCV C V SAS A + C + + C ++ V
Sbjct: 1 MASMVMNVLCVAVACMV---FSASY----ADAISCGQVTSGLVPCFGYLAAGGPVPP--- 50
Query: 61 QCCS---GLKSVLKTAP--SCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVT-NC 114
CC+ GL + KT P C K +N+ A +LP C +S +T N
Sbjct: 51 ACCNGVRGLNNAAKTTPDRQTACGCLKGILAANTRINLNNANSLPGKCGISIGYKITPNI 110
Query: 115 GC 116
C
Sbjct: 111 DC 112
>NLTP_MAIZE (P19656) Nonspecific lipid-transfer protein precursor
(LTP) (Phospholipid transfer protein) (PLTP) (Allergen
Zea m 14)
Length = 120
Score = 38.1 bits (87), Expect = 0.012
Identities = 28/109 (25%), Positives = 48/109 (43%), Gaps = 9/109 (8%)
Query: 9 LCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKS 68
L VVA V A++ + A S C + + C+S+ + P CCSG++S
Sbjct: 7 LAVVATAVVALVLLAAATSEAAIS--CGQVASAIAPCISYARGQGS--GPSAGCCSGVRS 62
Query: 69 VLKTAPS-----CLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVT 112
+ A + C K+++ LN A ++PS C VS +++
Sbjct: 63 LNNAARTTADRRAACNCLKNAAAGVSGLNAGNAASIPSKCGVSIPYTIS 111
>NL43_HORVU (Q42842) Nonspecific lipid-transfer protein 4.3
precursor (LTP 4.3)
Length = 115
Score = 38.1 bits (87), Expect = 0.012
Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 10/83 (12%)
Query: 35 CSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS-----CLCEAFKSSSQFGV 89
C + ++ C+S+ + KP CCSG+K + A S C+ KS++
Sbjct: 29 CGQVSSALSPCISYARGNGA--KPPVACCSGVKRLAGAAQSTADKQAACKCIKSAAG--- 83
Query: 90 VLNVTKALALPSACKVSASNSVT 112
LN KA +PS C VS +++
Sbjct: 84 GLNAGKAAGIPSMCGVSVPYAIS 106
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.328 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,755,506
Number of Sequences: 164201
Number of extensions: 714314
Number of successful extensions: 2018
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 62
Number of HSP's that attempted gapping in prelim test: 1992
Number of HSP's gapped (non-prelim): 67
length of query: 185
length of database: 59,974,054
effective HSP length: 104
effective length of query: 81
effective length of database: 42,897,150
effective search space: 3474669150
effective search space used: 3474669150
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0314.16