BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.12
(180 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ATOX_RAT (Q9WUC4) Copper transport protein ATOX1 (Metal transpor... 39 0.005
ATOX_MOUSE (O08997) Copper transport protein ATOX1 (Metal transp... 39 0.005
CCS1_YEAST (P40202) Superoxide dismutase 1 copper chaperone 39 0.007
ATOX_SHEEP (Q9XT28) Copper transport protein ATOX1 (Metal transp... 39 0.008
CAD2_LISMO (Q60048) Probable cadmium-transporting ATPase (EC 3.6... 33 0.27
CAD1_LISMO (P58414) Probable cadmium-transporting ATPase (EC 3.6... 33 0.27
ATCU_SALTY (Q8ZR95) Copper-transporting P-type ATPase (EC 3.6.3.4) 33 0.36
ATCU_SALTI (Q8Z8S4) Copper-transporting P-type ATPase (EC 3.6.3.4) 33 0.36
YJB1_SCHPO (O74939) Hypothetical protein C553.01c in chromosome III 33 0.47
FIXI_BRAJA (Q59207) Nitrogen fixation protein fixI (E1-E2 type c... 33 0.47
CENF_HUMAN (P49454) CENP-F kinetochore protein (Centromere prote... 33 0.47
ATOX_HUMAN (O00244) Copper transport protein ATOX1 (Metal transp... 33 0.47
ATCU_ECOLI (Q59385) Copper-transporting P-type ATPase (EC 3.6.3.4) 33 0.47
ATCU_ECO57 (Q8XD24) Copper-transporting P-type ATPase (EC 3.6.3.4) 33 0.47
ATX1_YEAST (P38636) Metal homeostasis factor ATX1 32 0.61
VATD_NEUCR (O59941) Vacuolar ATP synthase subunit D (EC 3.6.3.14... 32 1.0
ELA1_RAT (Q63187) Transcription elongation factor B polypeptide ... 32 1.0
ELA1_MOUSE (Q8CB77) Transcription elongation factor B polypeptid... 32 1.0
COPP_HELPY (Q48271) COP associated protein (Copper ion binding p... 32 1.0
PIB4_BOVIN (Q07722) 1-phosphatidylinositol-4,5-bisphosphate phos... 31 1.4
>ATOX_RAT (Q9WUC4) Copper transport protein ATOX1 (Metal transport
protein ATX1)
Length = 68
Score = 39.3 bits (90), Expect = 0.005
Identities = 22/60 (36%), Positives = 36/60 (59%), Gaps = 1/60 (1%)
Query: 121 VAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFWNG 180
V + C GCA V R L+K+ GV FNID+ +K+V + S ++L +++K +A + G
Sbjct: 8 VDMTCGGCAEAVSRVLNKLGGV-EFNIDLPNKKVCIESEHSSDILLATLNKTGKAVSYLG 66
>ATOX_MOUSE (O08997) Copper transport protein ATOX1 (Metal transport
protein ATX1)
Length = 68
Score = 39.3 bits (90), Expect = 0.005
Identities = 22/60 (36%), Positives = 36/60 (59%), Gaps = 1/60 (1%)
Query: 121 VAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFWNG 180
V + C+GCA V R L+K+ GV FNID+ +K+V + S +L +++K +A + G
Sbjct: 8 VDMTCEGCAEAVSRVLNKLGGV-EFNIDLPNKKVCIDSEHSSDTLLATLNKTGKAVSYLG 66
>CCS1_YEAST (P40202) Superoxide dismutase 1 copper chaperone
Length = 249
Score = 38.9 bits (89), Expect = 0.007
Identities = 12/49 (24%), Positives = 28/49 (56%)
Query: 121 VAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESI 169
+ +HC+ C +K L + G+ S N D+E + ++V ++P ++ ++
Sbjct: 13 IPMHCENCVNDIKACLKNVPGINSLNFDIEQQIMSVESSVAPSTIINTL 61
>ATOX_SHEEP (Q9XT28) Copper transport protein ATOX1 (Metal transport
protein ATX1) (Copper chaperone SAH)
Length = 68
Score = 38.5 bits (88), Expect = 0.008
Identities = 21/60 (35%), Positives = 36/60 (60%), Gaps = 1/60 (1%)
Query: 121 VAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFWNG 180
V + C+GC+ V R L+K+ GV F+ID+ +K+V + S +LE++ K +A + G
Sbjct: 8 VDMTCEGCSNAVTRVLNKLGGV-QFDIDLPNKKVCINSEHSVDTLLETLGKTGKAVSYLG 66
>CAD2_LISMO (Q60048) Probable cadmium-transporting ATPase (EC
3.6.3.3) (Cadmium efflux ATPase)
Length = 711
Score = 33.5 bits (75), Expect = 0.27
Identities = 13/39 (33%), Positives = 24/39 (61%)
Query: 123 IHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHIS 161
+ C CA K +R++ ++EGVT ++ + ++TV G S
Sbjct: 12 LSCTNCAAKFERNVKEIEGVTEAIVNFGASKITVTGEAS 50
>CAD1_LISMO (P58414) Probable cadmium-transporting ATPase (EC
3.6.3.3) (Cadmium efflux ATPase)
Length = 707
Score = 33.5 bits (75), Expect = 0.27
Identities = 15/51 (29%), Positives = 30/51 (58%), Gaps = 7/51 (13%)
Query: 125 CQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRA 175
C CAGK ++++ +EGVT ++ + +++V G SIS++++A
Sbjct: 17 CTNCAGKFEKNVKNLEGVTDAKVNFGAGKISVYGE-------TSISQIEKA 60
>ATCU_SALTY (Q8ZR95) Copper-transporting P-type ATPase (EC 3.6.3.4)
Length = 832
Score = 33.1 bits (74), Expect = 0.36
Identities = 17/70 (24%), Positives = 33/70 (46%), Gaps = 1/70 (1%)
Query: 102 PKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHIS 161
P EL D Q +++ + C C +V+ L + GVT +++ + VMG S
Sbjct: 86 PHELPVATADEESQQLLLS-GMSCASCVTRVQHALQSVPGVTQARVNLAERTALVMGSAS 144
Query: 162 PVVVLESISK 171
+++++ K
Sbjct: 145 AADLVQAVEK 154
>ATCU_SALTI (Q8Z8S4) Copper-transporting P-type ATPase (EC 3.6.3.4)
Length = 832
Score = 33.1 bits (74), Expect = 0.36
Identities = 17/70 (24%), Positives = 33/70 (46%), Gaps = 1/70 (1%)
Query: 102 PKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHIS 161
P EL D Q +++ + C C +V+ L + GVT +++ + VMG S
Sbjct: 86 PHELPVATADEESQQLLLS-GMSCASCVTRVQHALQSVPGVTQARVNLAERTALVMGSAS 144
Query: 162 PVVVLESISK 171
+++++ K
Sbjct: 145 AADLVQAVEK 154
>YJB1_SCHPO (O74939) Hypothetical protein C553.01c in chromosome III
Length = 715
Score = 32.7 bits (73), Expect = 0.47
Identities = 28/110 (25%), Positives = 50/110 (45%), Gaps = 15/110 (13%)
Query: 67 VQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQVVVMRVAIHCQ 126
V + + SS +++S P ++ NL + + +PPK L K P ++F
Sbjct: 595 VPNAIETDSSPSDTISSSPTVRAVNLSPSKNQ-KPPKILTKIP--DIFSK---------- 641
Query: 127 GCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAE 176
AGK L + S I+ S +T + ++ V + + SK+KRA+
Sbjct: 642 --AGKKSPILPTFQESCSLPIEPFSSNITDLPFVNTVTLASTSSKIKRAK 689
>FIXI_BRAJA (Q59207) Nitrogen fixation protein fixI (E1-E2 type
cation ATPase fixI) (EC 3.6.3.-)
Length = 730
Score = 32.7 bits (73), Expect = 0.47
Identities = 13/50 (26%), Positives = 27/50 (54%)
Query: 107 KTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTV 156
+T + + + + +HC GC K++R LS + VT +++ +RV +
Sbjct: 12 RTAGEGIKHIDLAVEGVHCAGCMAKIERGLSAIPDVTLARVNLTDRRVAL 61
>CENF_HUMAN (P49454) CENP-F kinetochore protein (Centromere protein F)
(Mitosin) (AH antigen)
Length = 3210
Score = 32.7 bits (73), Expect = 0.47
Identities = 25/110 (22%), Positives = 51/110 (45%), Gaps = 6/110 (5%)
Query: 70 RSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQVVVMRVAIHCQGCA 129
RS T + S S +KQ + EE+N+ KE+ +N + VM+ CQ
Sbjct: 1111 RSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEVNDLLQENEQLMKVMKTKHECQNLE 1170
Query: 130 GK-VKRHLSKMEGVTSF-----NIDVESKRVTVMGHISPVVVLESISKVK 173
+ ++ + + E + +D+E K +++ + + +V LE++ + K
Sbjct: 1171 SEPIRNSVKERESERNQCNFKPQMDLEVKEISLDSYNAQLVQLEAMLRNK 1220
>ATOX_HUMAN (O00244) Copper transport protein ATOX1 (Metal transport
protein ATX1)
Length = 68
Score = 32.7 bits (73), Expect = 0.47
Identities = 19/60 (31%), Positives = 33/60 (54%), Gaps = 1/60 (1%)
Query: 121 VAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFWNG 180
V + C GCA V R L+K+ GV ++ID+ +K+V + S +L ++ K + + G
Sbjct: 8 VDMTCGGCAEAVSRVLNKLGGV-KYDIDLPNKKVCIESEHSMDTLLATLKKTGKTVSYLG 66
>ATCU_ECOLI (Q59385) Copper-transporting P-type ATPase (EC 3.6.3.4)
Length = 833
Score = 32.7 bits (73), Expect = 0.47
Identities = 13/47 (27%), Positives = 25/47 (52%)
Query: 125 CQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISK 171
C C +V+ L + GVT +++ + VMG SP +++++ K
Sbjct: 109 CASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEK 155
>ATCU_ECO57 (Q8XD24) Copper-transporting P-type ATPase (EC 3.6.3.4)
Length = 833
Score = 32.7 bits (73), Expect = 0.47
Identities = 13/47 (27%), Positives = 25/47 (52%)
Query: 125 CQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISK 171
C C +V+ L + GVT +++ + VMG SP +++++ K
Sbjct: 109 CASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEK 155
>ATX1_YEAST (P38636) Metal homeostasis factor ATX1
Length = 73
Score = 32.3 bits (72), Expect = 0.61
Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Query: 121 VAIHCQGCAGKVKRHLSKME-GVTSFNIDVESKRVTVMGHISPVVVLESISK 171
V + C GC+G V + L+K+E V+ +I +E + V V + +LE I K
Sbjct: 11 VVMTCSGCSGAVNKVLTKLEPDVSKIDISLEKQLVDVYTTLPYDFILEKIKK 62
>VATD_NEUCR (O59941) Vacuolar ATP synthase subunit D (EC 3.6.3.14)
(V-ATPase D subunit) (Vacuolar proton pump D subunit)
Length = 266
Score = 31.6 bits (70), Expect = 1.0
Identities = 19/100 (19%), Positives = 44/100 (44%), Gaps = 13/100 (13%)
Query: 37 VVVPKRHDRTIFVDDDHTRLINYANKYSKLVQSRSRSTSSDRESVSRVPKIKQRNLDQVE 96
V++P+ + +++ + L K V ++ + +++ ++ + K +Q+ L +
Sbjct: 178 VIIPRTENTIKYINSELDELDREEFYRLKKVAAKKQRDNAETDAQMKAKKAEQQRLALAD 237
Query: 97 EENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHL 136
EN ++ + TP D + GC G +RHL
Sbjct: 238 SENAEGEQTENTPADIL-------------GCRGGRRRHL 264
>ELA1_RAT (Q63187) Transcription elongation factor B polypeptide 3
(RNA polymerase II transcription factor SIII subunit A)
(SIII p110) (Elongin A) (EloA) (Elongin 110 kDa subunit)
Length = 773
Score = 31.6 bits (70), Expect = 1.0
Identities = 14/46 (30%), Positives = 24/46 (51%)
Query: 66 LVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTD 111
L + S+STS + S ++PK + D+++ P +K PTD
Sbjct: 432 LKKKDSKSTSKNLNSAQKLPKANENKSDKLQPAGAEPTRPRKVPTD 477
>ELA1_MOUSE (Q8CB77) Transcription elongation factor B polypeptide 3
(RNA polymerase II transcription factor SIII subunit A)
(SIII p110) (Elongin A) (EloA) (Elongin 110 kDa subunit)
Length = 773
Score = 31.6 bits (70), Expect = 1.0
Identities = 13/46 (28%), Positives = 25/46 (54%)
Query: 66 LVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTD 111
L + S+STS + S ++PK+ + ++++ P +K PTD
Sbjct: 432 LKKKDSKSTSKNLNSAQKLPKVNENKSEKLQPAGAEPTRPRKVPTD 477
>COPP_HELPY (Q48271) COP associated protein (Copper ion binding
protein)
Length = 66
Score = 31.6 bits (70), Expect = 1.0
Identities = 12/35 (34%), Positives = 22/35 (62%)
Query: 122 AIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTV 156
+I C C K+++ + ++EGV+ ++ VE K V V
Sbjct: 9 SITCNHCVDKIEKFVGEIEGVSFIDVSVEKKSVVV 43
>PIB4_BOVIN (Q07722) 1-phosphatidylinositol-4,5-bisphosphate
phosphodiesterase beta 4 (EC 3.1.4.11) (Phosphoinositide
phospholipase C) (PLC-beta-4) (Phospholipase C-beta-4)
(PCL-C1) (Fragment)
Length = 1023
Score = 31.2 bits (69), Expect = 1.4
Identities = 15/53 (28%), Positives = 31/53 (58%), Gaps = 1/53 (1%)
Query: 55 RLINYANKYSKLVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQK 107
R +N +N K ++ R R + + ++ K++ +L+ VE++NE KE+Q+
Sbjct: 956 RELNSSNT-KKFLEERKRLAMKQSKEMDQLKKVQLEHLEFVEKQNEQAKEMQQ 1007
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.129 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,537,825
Number of Sequences: 164201
Number of extensions: 659663
Number of successful extensions: 2483
Number of sequences better than 10.0: 60
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 31
Number of HSP's that attempted gapping in prelim test: 2441
Number of HSP's gapped (non-prelim): 72
length of query: 180
length of database: 59,974,054
effective HSP length: 103
effective length of query: 77
effective length of database: 43,061,351
effective search space: 3315724027
effective search space used: 3315724027
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0314.12