BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0307.15
(326 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
VDP_MOUSE (Q9Z1Z0) General vesicular transport factor p115 (Tran... 40 0.010
VDP_HUMAN (O60763) General vesicular transport factor p115 (Tran... 39 0.014
VDP_RAT (P41542) General vesicular transport factor p115 (Transc... 38 0.030
VDP_BOVIN (P41541) General vesicular transport factor p115 (Tran... 37 0.088
GBP5_HUMAN (Q96PP8) Interferon-induced guanylate-binding protein... 35 0.26
MYSA_DROME (P05661) Myosin heavy chain, muscle 35 0.33
YKR9_YEAST (P34237) Hypothetical 77.5 kDa protein in RPL17A-STE3... 34 0.44
YKI5_CAEEL (P46012) Hypothetical protein C01G6.5 in chromosome III 33 0.75
TLN1_MOUSE (P26039) Talin 1 33 0.97
NMCP_ARATH (Q9FLH0) Putative nuclear matrix constituent protein ... 33 1.3
MYS2_SCHPO (Q9USI6) Myosin type II heavy chain 1 33 1.3
CENE_HUMAN (Q02224) Centromeric protein E (CENP-E protein) 33 1.3
TLN1_HUMAN (Q9Y490) Talin 1 32 1.7
CIN8_ASHGO (Q8J1G7) Kinesin-like protein CIN8 32 1.7
SMC1_YEAST (P32908) Structural maintenance of chromosome 1 (DA-b... 32 2.2
RNR_MYCPU (Q98QL0) Ribonuclease R (EC 3.1.-.-) (RNase R) (VacB p... 32 2.2
YMV5_YEAST (Q04746) Hypothetical 58.4 kDa protein in NCA1-HMS1 i... 32 2.8
GCC2_HUMAN (Q8IWJ2) GRIP and coiled-coil domain-containing prote... 32 2.8
C5P1_HUMAN (Q96SZ6) CDK5 regulatory subunit-associated protein 1... 32 2.8
TYCC_BREPA (O30409) Tyrocidine synthetase III [Includes: ATP-dep... 31 3.7
>VDP_MOUSE (Q9Z1Z0) General vesicular transport factor p115
(Transcytosis associated protein) (TAP) (Vesicle docking
protein) (Fragment)
Length = 941
Score = 39.7 bits (91), Expect = 0.010
Identities = 30/105 (28%), Positives = 51/105 (48%), Gaps = 9/105 (8%)
Query: 229 DVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSS--------SESSKLELALMHQWK 280
DV+GRLSAL + K+ N ++ LSE + ++ SS +E KL+L + K
Sbjct: 827 DVEGRLSALLQETKELKNEIKALSEERTAIQKQLDSSNSTIAILQTEKDKLDLEVTDSKK 886
Query: 281 SLFDFMENTFAEESRQLESSFETLKDLRDNCKNDLDSTDNTHFDN 325
D + A++ +++ S LKDL + + +S D D+
Sbjct: 887 EQDDLLV-LLADQDQKILSLKSKLKDLGHPVEEEDESGDQEDDDD 930
>VDP_HUMAN (O60763) General vesicular transport factor p115
(Transcytosis associated protein) (TAP) (Vesicle docking
protein)
Length = 962
Score = 39.3 bits (90), Expect = 0.014
Identities = 32/107 (29%), Positives = 53/107 (48%), Gaps = 11/107 (10%)
Query: 229 DVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSS--------SESSKLELALMHQWK 280
DV+GRLSAL + K+ N ++ LSE + ++ SS +E KLEL + K
Sbjct: 845 DVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKK 904
Query: 281 SLFDFMENTFAEESRQLESSFETLKDLRDNC--KNDLDSTDNTHFDN 325
D + A++ +++ S LKDL +++L+S D D+
Sbjct: 905 EQDDLLV-LLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDD 950
>VDP_RAT (P41542) General vesicular transport factor p115
(Transcytosis associated protein) (TAP) (Vesicle docking
protein)
Length = 959
Score = 38.1 bits (87), Expect = 0.030
Identities = 30/105 (28%), Positives = 50/105 (47%), Gaps = 9/105 (8%)
Query: 229 DVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSS--------SESSKLELALMHQWK 280
DV+GRLSAL + K+ N ++ LSE + ++ SS +E KL L + K
Sbjct: 845 DVEGRLSALLQETKELKNEIKALSEERTAIQKQLDSSNSTIAILQTEKDKLYLEVTDSKK 904
Query: 281 SLFDFMENTFAEESRQLESSFETLKDLRDNCKNDLDSTDNTHFDN 325
D + A++ +++ S LKDL + + +S D D+
Sbjct: 905 EQDDLLV-LLADQDQKILSLKSKLKDLGHPVEEEDESGDQEDDDD 948
>VDP_BOVIN (P41541) General vesicular transport factor p115
(Transcytosis associated protein) (TAP) (Vesicle docking
protein)
Length = 961
Score = 36.6 bits (83), Expect = 0.088
Identities = 30/107 (28%), Positives = 54/107 (50%), Gaps = 11/107 (10%)
Query: 229 DVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSS--------SESSKLELALMHQWK 280
DV+GRLSAL + K+ N ++ LSE + ++ SS +E +KLE+ + K
Sbjct: 844 DVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQNEKNKLEVDITDSKK 903
Query: 281 SLFDFMENTFAEESRQLESSFETLKDLRDNC--KNDLDSTDNTHFDN 325
D + A++ +++ S LK+L +++L+S D D+
Sbjct: 904 EQDDLLV-LLADQDQKIFSLKNKLKELGHPVEEEDELESGDQDDEDD 949
>GBP5_HUMAN (Q96PP8) Interferon-induced guanylate-binding protein 5
(GTP-binding protein 5) (Guanine nucleotide-binding
protein 5) (GBP-TA antigen) (UNQ2427/PRO4987)
Length = 586
Score = 35.0 bits (79), Expect = 0.26
Identities = 24/91 (26%), Positives = 46/91 (50%), Gaps = 5/91 (5%)
Query: 235 SALKKSQKDASNAVQKLSEIKDSVMPDQQSSSESSKLELALMHQW---KSLFDFMENTFA 291
+ L +Q++ S AVQK D M + + EL +H+ +++ FM+N+F
Sbjct: 313 AVLALAQRENSAAVQKAIAHYDQQMGQKVQLPMETLQELLDLHRTSEREAIEVFMKNSFK 372
Query: 292 EESRQLESSFETLKDLRDN--CKNDLDSTDN 320
+ + + ETL D + N CK +L+++ +
Sbjct: 373 DVDQSFQKELETLLDAKQNDICKRNLEASSD 403
>MYSA_DROME (P05661) Myosin heavy chain, muscle
Length = 1962
Score = 34.7 bits (78), Expect = 0.33
Identities = 22/98 (22%), Positives = 42/98 (42%)
Query: 227 YQDVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSSSESSKLELALMHQWKSLFDFM 286
+ D+ L+ K S++ A A+ + + D + +Q + KL AL Q K L +
Sbjct: 1737 HSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRL 1796
Query: 287 ENTFAEESRQLESSFETLKDLRDNCKNDLDSTDNTHFD 324
+ A + + + + L+ +N+LD H D
Sbjct: 1797 DEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHAD 1834
>YKR9_YEAST (P34237) Hypothetical 77.5 kDa protein in RPL17A-STE3
intergenic region
Length = 679
Score = 34.3 bits (77), Expect = 0.44
Identities = 30/108 (27%), Positives = 52/108 (47%), Gaps = 13/108 (12%)
Query: 227 YQDVKGRLSALKK-----SQKDASNAVQKLSEIKDSVMPDQQSSSESSKLELALMHQWKS 281
Y +K LSALKK ++ D+ N ++ SE K+ + S + KL+ A + +++S
Sbjct: 342 YNKIKEELSALKKIEFGVNEDDSDNDIR--SEDKNDNTFESSLLSANKKLQ-ATLAEYRS 398
Query: 282 LFDFME---NTFAEESRQLESSFETLKDLRDNCKNDLDSTDNT--HFD 324
E N + QL+ TLK+ + + DL+ +N HF+
Sbjct: 399 KSTAQEEERNELKKSVDQLKQQIATLKEANEKLETDLEKVENVSPHFN 446
>YKI5_CAEEL (P46012) Hypothetical protein C01G6.5 in chromosome III
Length = 952
Score = 33.5 bits (75), Expect = 0.75
Identities = 24/109 (22%), Positives = 48/109 (44%), Gaps = 5/109 (4%)
Query: 166 GRAVELPTMSDSCDSRFPGLERCSVLQGNAGPTSAAAAADLLKSCTCSFCSKAAHIW--- 222
GR PT + P ERC V +G+ + L+ +CS C++ H+W
Sbjct: 844 GRKKATPTEEEEPPKTEPSKERCGVAKGHCISAKYEKRKN-LQWVSCSICNQWFHVWCVR 902
Query: 223 -SDLHYQDVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSSSESSK 270
++ Y++ + L K+A +A+ + MP+++ ++S+
Sbjct: 903 LDNVCYREDETFLCCGSHPSKEAKDALAGRVYARYQAMPNKKPIPQASE 951
>TLN1_MOUSE (P26039) Talin 1
Length = 2541
Score = 33.1 bits (74), Expect = 0.97
Identities = 49/208 (23%), Positives = 72/208 (34%), Gaps = 38/208 (18%)
Query: 72 QKGQNPCLELPFCGDDSLGNVSSQESNLQ-------------LPSGGLEEKNLLGGAVVS 118
QK Q C L D +L V + E +LQ LP +E+ G
Sbjct: 1039 QKAQEACGPLEM--DSALSVVQNLEKDLQEIKAAARDGKLKPLPGETMEKCTQDLGNSTK 1096
Query: 119 GPTEVPVAVLGEIGQGRPLNEGFIDRGIESGQRDV----RAVDNLGSPKCGGRAVELPTM 174
+ +LGEI QG G R + G R + R V L S +A+ L T
Sbjct: 1097 AVSSAIAKLLGEIAQGNENYAGIAARDVAGGLRSLAQAARGVAALTSDP-AVQAIVLDTA 1155
Query: 175 SDSCDSRFPGLERCSVLQGNAG-PTSAAAAADLLKSCTCSFCSKAAHIWSDLHYQDVKGR 233
SD D +E G+ G P S A + K+ T R
Sbjct: 1156 SDVLDKASSLIEEAKKASGHPGDPESQQRLAQVAKAVT-----------------QALNR 1198
Query: 234 LSALKKSQKDASNAVQKLSEIKDSVMPD 261
+ Q+D NA++ + + ++ D
Sbjct: 1199 CVSCLPGQRDVDNALRAVGDASKRLLSD 1226
>NMCP_ARATH (Q9FLH0) Putative nuclear matrix constituent protein
1-like protein (NMCP1-like)
Length = 1042
Score = 32.7 bits (73), Expect = 1.3
Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 9/98 (9%)
Query: 233 RLSALKKSQKDASNAVQKLSEIKDSVMP---DQQSSSESSKLELALMH-QWKSLFDFMEN 288
+L ALK + S KL E D + + + ++ K+E A +W+ + D
Sbjct: 512 KLEALKSETSELSTLEMKLKEELDDLRAQKLEMLAEADRLKVEKAKFEAEWEHI-DVKRE 570
Query: 289 TFAEES----RQLESSFETLKDLRDNCKNDLDSTDNTH 322
+E+ RQ E+ LKD RDN K + D+ N H
Sbjct: 571 ELRKEAEYITRQREAFSMYLKDERDNIKEERDALRNQH 608
>MYS2_SCHPO (Q9USI6) Myosin type II heavy chain 1
Length = 1526
Score = 32.7 bits (73), Expect = 1.3
Identities = 24/87 (27%), Positives = 41/87 (46%), Gaps = 5/87 (5%)
Query: 231 KGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSSSESSKLELALMHQWKSLFDFMENTF 290
K +LS KD SN V L++ + + P E + LMH++ L TF
Sbjct: 1216 KEKLSLASSENKDLSNKVSSLTKQVNELSPKASKVPELERKITNLMHEYSQL----GKTF 1271
Query: 291 AEESRQLESSFETLKDLRDNCKNDLDS 317
+E R+ + ++LR + K++L+S
Sbjct: 1272 EDEKRKALIASRDNEELR-SLKSELES 1297
>CENE_HUMAN (Q02224) Centromeric protein E (CENP-E protein)
Length = 2663
Score = 32.7 bits (73), Expect = 1.3
Identities = 26/93 (27%), Positives = 43/93 (45%), Gaps = 2/93 (2%)
Query: 228 QDVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSSSESSKLELALMHQWKSLFDFME 287
Q++K L +KD + +K+SE++ + S SKLE+ ++ + L + +E
Sbjct: 1823 QELKANEHQLITLKKDVNETQKKVSEMEQLKKQIKDQSLTLSKLEIENLNLAQELHENLE 1882
Query: 288 --NTFAEESRQLESSFETLKDLRDNCKNDLDST 318
+ +E L ETLK RD K L T
Sbjct: 1883 EMKSVMKERDNLRRVEETLKLERDQLKESLQET 1915
>TLN1_HUMAN (Q9Y490) Talin 1
Length = 2541
Score = 32.3 bits (72), Expect = 1.7
Identities = 48/208 (23%), Positives = 72/208 (34%), Gaps = 38/208 (18%)
Query: 72 QKGQNPCLELPFCGDDSLGNVSSQESNLQ-------------LPSGGLEEKNLLGGAVVS 118
QK Q C L D +L V + E +LQ LP +E+ G
Sbjct: 1039 QKAQEACGPLEM--DSALSVVQNLEKDLQEVKAAARDGKLKPLPGETMEKCTQDLGNSTK 1096
Query: 119 GPTEVPVAVLGEIGQGRPLNEGFIDRGIESGQRDV----RAVDNLGSPKCGGRAVELPTM 174
+ +LGE+ QG G R + G R + R V L S +A+ L T
Sbjct: 1097 AVSSAIAQLLGEVAQGNENYAGIAARDVAGGLRSLAQAARGVAALTSDP-AVQAIVLDTA 1155
Query: 175 SDSCDSRFPGLERCSVLQGNAG-PTSAAAAADLLKSCTCSFCSKAAHIWSDLHYQDVKGR 233
SD D +E G+ G P S A + K+ T R
Sbjct: 1156 SDVLDKASSLIEEAKKAAGHPGDPESQQRLAQVAKAVT-----------------QALNR 1198
Query: 234 LSALKKSQKDASNAVQKLSEIKDSVMPD 261
+ Q+D NA++ + + ++ D
Sbjct: 1199 CVSCLPGQRDVDNALRAVGDASKRLLSD 1226
>CIN8_ASHGO (Q8J1G7) Kinesin-like protein CIN8
Length = 945
Score = 32.3 bits (72), Expect = 1.7
Identities = 27/120 (22%), Positives = 59/120 (48%), Gaps = 6/120 (5%)
Query: 207 LKSCTCSFCSKAAHIWSDLHYQDVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSSS 266
+KS S SK S HYQ++ L + +++ ++ L+ + ++ D+++S
Sbjct: 435 IKSDFLSTKSKDGIYMSHEHYQEIVNDLENCQTEIQESKRQIESLTSQNNLLLKDKKASQ 494
Query: 267 ESSKLELALMHQWKS----LFDFMENTFAEESRQLESSFETLKDLRDNCKNDLDSTDNTH 322
E ++L+ + + + +S L+D +E E+ +L ++ LK+ + L S + TH
Sbjct: 495 EVTELQNSKIKKLQSTIEYLYDKIERQHHNET-ELATTIHKLKEALHTMQGSLKSYE-TH 552
>SMC1_YEAST (P32908) Structural maintenance of chromosome 1 (DA-box
protein SMC1)
Length = 1225
Score = 32.0 bits (71), Expect = 2.2
Identities = 24/93 (25%), Positives = 44/93 (46%), Gaps = 16/93 (17%)
Query: 238 KKSQKDASNAVQKLSEIKDSVMP-DQQSSSESSKLELALMHQWKSLFDFMENTFAEESRQ 296
+K+QKD NA ++ +++ + + S SKLE + K+ D ++ F + +
Sbjct: 847 EKAQKDLENAQVEMKSLEEQEYAIEMKIGSIESKLE-----EHKNHLDELQKKFVTKQSE 901
Query: 297 LESSFETLKDL----------RDNCKNDLDSTD 319
L SS + L+D+ RD K D++ D
Sbjct: 902 LNSSEDILEDMNSNLQVLKRERDGIKEDIEKFD 934
>RNR_MYCPU (Q98QL0) Ribonuclease R (EC 3.1.-.-) (RNase R) (VacB
protein homolog)
Length = 725
Score = 32.0 bits (71), Expect = 2.2
Identities = 25/89 (28%), Positives = 41/89 (45%), Gaps = 15/89 (16%)
Query: 241 QKDASNAVQKLSEIKDSVMPDQQSSSESSKLELALMHQWKSLFDFMENTFAE----ESRQ 296
QK N V+K+S +K S ++++ L + K++ DF E+ E E
Sbjct: 180 QKIYVNFVKKVSTLK----------SNFYQIDIVL-EKSKTIIDFPEDVLDESALIEDHV 228
Query: 297 LESSFETLKDLRDNCKNDLDSTDNTHFDN 325
+E ++ KDLRD +D D FD+
Sbjct: 229 IEKDYQNRKDLRDKLIITIDGEDTKDFDD 257
>YMV5_YEAST (Q04746) Hypothetical 58.4 kDa protein in NCA1-HMS1
intergenic region
Length = 504
Score = 31.6 bits (70), Expect = 2.8
Identities = 20/91 (21%), Positives = 41/91 (44%), Gaps = 3/91 (3%)
Query: 232 GRLSALKKSQKDASNAVQKLSEI-KDSVMPDQQSS--SESSKLELALMHQWKSLFDFMEN 288
G L +SQ +A +Q + I DS++P E + + L+ +W ++ +
Sbjct: 324 GMSKDLSESQMEAIELLQGFNSILHDSLLPSMTDEIVPEMTNFKNTLLQEWTAITSTLNG 383
Query: 289 TFAEESRQLESSFETLKDLRDNCKNDLDSTD 319
FA + ++ S+F + + + K LD +
Sbjct: 384 DFALWNEEIFSTFNDISEKLNGTKKKLDDIE 414
>GCC2_HUMAN (Q8IWJ2) GRIP and coiled-coil domain-containing protein
2 (Golgi coiled coil protein GCC185) (CTCL tumor antigen
se1-1) (CLL-associated antigen KW-11)
Length = 1583
Score = 31.6 bits (70), Expect = 2.8
Identities = 23/98 (23%), Positives = 51/98 (51%), Gaps = 15/98 (15%)
Query: 225 LHYQDVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSSSES--SKLELALMHQWKSL 282
+H ++VK + ++ S K+ + KL+E+K++++ ++S ++ K E L + K+
Sbjct: 146 IHQEEVKELMCQIEASAKEHEAEINKLNELKENLVKQCEASEKNIQKKYECELENLRKAT 205
Query: 283 FD-----------FMENTFAEE--SRQLESSFETLKDL 307
+ ENTF E+ + +++ +TLK+L
Sbjct: 206 SNANQDNQICSILLQENTFVEQVVNEKVKHLEDTLKEL 243
>C5P1_HUMAN (Q96SZ6) CDK5 regulatory subunit-associated protein 1
(CDK5 activator-binding protein C42) (CGI-05) (HSPC167)
Length = 601
Score = 31.6 bits (70), Expect = 2.8
Identities = 26/86 (30%), Positives = 44/86 (50%), Gaps = 5/86 (5%)
Query: 227 YQDVKGRLSALKKSQKDASNAVQKLSEIKDSVMPDQQSSSESSKLELALMHQWKSLFDFM 286
Y+D+ RL A+ +S + A+N + L E VMP Q S+S +S +++M ++ +
Sbjct: 208 YRDLP-RLLAVAESGQQAANVLLSLDETYADVMPVQTSASATSAF-VSIMRGCDNMCSYC 265
Query: 287 ENTFA---EESRQLESSFETLKDLRD 309
F E SR + S E +K L +
Sbjct: 266 IVPFTRGRERSRPIASILEEVKKLSE 291
>TYCC_BREPA (O30409) Tyrocidine synthetase III [Includes:
ATP-dependent asparagine adenylase (AsnA) (Asparagine
activase); ATP-dependent glutamine adenylase (GlnA)
(Glutamine activase); ATP-dependent tyrosine adenylase
(TyrA) (Tyrosine activase); ATP-depe
Length = 6486
Score = 31.2 bits (69), Expect = 3.7
Identities = 21/78 (26%), Positives = 38/78 (47%), Gaps = 8/78 (10%)
Query: 64 KDKVV---VSQQKGQNPCLELPFCGDDSLGNVSSQESNLQLPSGGLEEKNLLGGAVVS-- 118
+DK + +++Q + P CG+D+L E + QL + L EK + G++VS
Sbjct: 462 RDKTIFELIAEQASRTPAKAAVVCGEDTLTYQELMERSAQL-ANALREKGIASGSIVSIM 520
Query: 119 --GPTEVPVAVLGEIGQG 134
E+ VA++ + G
Sbjct: 521 AEHSLELIVAIMAVLRSG 538
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.311 0.129 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,201,157
Number of Sequences: 164201
Number of extensions: 1617064
Number of successful extensions: 3538
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 3522
Number of HSP's gapped (non-prelim): 45
length of query: 326
length of database: 59,974,054
effective HSP length: 110
effective length of query: 216
effective length of database: 41,911,944
effective search space: 9052979904
effective search space used: 9052979904
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0307.15