BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0302a.8
         (578 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TRA1_LACHE (P35880) Transposase for insertion sequence element I...    41  0.007
TRA6_BURCE (P24575) Transposase for insertion sequence element I...    34  0.91
SYV_ARCFU (O28059) Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--t...    34  0.91
TRA5_RHIME (Q52873) Transposase for insertion sequence element I...    33  2.0
OSTA_ECOLI (P31554) Organic solvent tolerance protein precursor        33  2.7
OSTA_ECOL6 (Q8CWE6) Organic solvent tolerance protein precursor        33  2.7
OSTA_ECO57 (Q8XA13) Organic solvent tolerance protein precursor        33  2.7
TRA6_STAAU (P19775) Transposase for insertion sequence element I...    32  5.9
TRA6_ENTFA (P59787) Transposase for insertion sequence element I...    32  5.9
2ABA_CANTR (P53031) Protein phosphatase PP2A regulatory subunit ...    31  7.7

>TRA1_LACHE (P35880) Transposase for insertion sequence element
           IS1201
          Length = 369

 Score = 41.2 bits (95), Expect = 0.007
 Identities = 27/105 (25%), Positives = 45/105 (42%), Gaps = 5/105 (4%)

Query: 245 VAFDATYGKNRYKC----PFVVFYGVNHHNKSTIFSTALVSNEKIETYVWLLERFLEAMK 300
           V  DATY   R +        +  G+  +    +    +  NE IE +  LL+       
Sbjct: 160 VYLDATYVPLRRETFEREAVYIAIGIKPNGHKEVIDYCIAPNENIEVWTELLQSMKSRGL 219

Query: 301 GKAPLFVITDGDKAMKAAIKQVFPNAHHRLCAWHILDNARKHVHI 345
            +  LF ++DG   MK A+ + +P AH + C  H++ N    V +
Sbjct: 220 EQVELF-LSDGVVGMKTALAKTYPQAHFQRCLVHVMRNICAKVRV 263


>TRA6_BURCE (P24575) Transposase for insertion sequence element
           IS406
          Length = 388

 Score = 34.3 bits (77), Expect = 0.91
 Identities = 25/100 (25%), Positives = 43/100 (43%), Gaps = 12/100 (12%)

Query: 248 DATYGKNRYK-----CPFVVFYGVNHHNKSTIFSTALVSNEKIETYVWLLERFLEAMKG- 301
           DA Y K R +     C  ++  G+    K  +    + ++E    +     RFLE++   
Sbjct: 166 DARYEKVRLEGRIVDCAVLIAVGIEASGKRRVLGCEVATSEAEINW----RRFLESLLAR 221

Query: 302 --KAPLFVITDGDKAMKAAIKQVFPNAHHRLCAWHILDNA 339
             K    +I D    +KAA + V P+   + C +H+  NA
Sbjct: 222 GLKGVTLIIADDHAGLKAARRAVLPSVPWQRCQFHLQQNA 261


>SYV_ARCFU (O28059) Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--tRNA
           ligase) (ValRS)
          Length = 863

 Score = 34.3 bits (77), Expect = 0.91
 Identities = 47/210 (22%), Positives = 83/210 (39%), Gaps = 25/210 (11%)

Query: 127 IAAHRKMNESDIMHMNNMSRSGISTP-QIHNTFASQTGGYQK-------VRFSKGNMYNV 178
           IA +++MN  ++M        G+ T  ++   +  + G   +       V F++ N+  +
Sbjct: 63  IARYKRMNGYEVMFPQGWDCHGLPTEVKVEEKYGIKKGDIPRDEFRRLCVEFTEENIAKM 122

Query: 179 QGRQRRMK-----KKE-----PE---KTDAEFAISYIRGLGSKD--PLLYCRHVANDDGN 223
           +   RRM       KE     PE   KT   F   Y +GL  +D  P+++C         
Sbjct: 123 RETARRMGYSIDWSKEYITMYPEYYSKTQLSFVRMYNKGLIYRDYHPVVFCPRCETTIA- 181

Query: 224 LDRLFWSDGISQLNYQVFGDVVAFDATYGKNRYKCPFVVFYGVNHHNKSTIFSTALVSNE 283
           L  + +  G ++LNY  F D V    T  +    C  +  +  +  NK  I     V   
Sbjct: 182 LAEIEYRQGKTKLNYIKFDDDVIIATTRPELIPACVAIAVHPDDERNKHLIGKKVRVPTT 241

Query: 284 KIETYVWLLERFLEAMKGKAPLFVITDGDK 313
             E  V + +  ++   G   + + T GD+
Sbjct: 242 PYEVEV-IADEEVDPEFGTGVVMICTFGDR 270


>TRA5_RHIME (Q52873) Transposase for insertion sequence element
           ISRM5
          Length = 398

 Score = 33.1 bits (74), Expect = 2.0
 Identities = 23/86 (26%), Positives = 38/86 (43%), Gaps = 7/86 (8%)

Query: 261 VVFYGVNHHNKSTIFSTALVSNEKIETYVWLLERFLEAMKG---KAPLFVITDGDKAMKA 317
           ++  G++   +  I S  +   E    +    + FL  +KG   K    V++D    + A
Sbjct: 182 LIAVGIDWDGRRQILSVEMAGRESRSAW----KDFLVRLKGRGLKGVELVVSDDHAGLVA 237

Query: 318 AIKQVFPNAHHRLCAWHILDNARKHV 343
           AI +V P A  + C  H L NA  H+
Sbjct: 238 AIGEVIPEAAWQRCYVHFLRNALDHL 263


>OSTA_ECOLI (P31554) Organic solvent tolerance protein precursor
          Length = 784

 Score = 32.7 bits (73), Expect = 2.7
 Identities = 33/134 (24%), Positives = 51/134 (37%), Gaps = 14/134 (10%)

Query: 108 KLFSDEHNHELEDDKMCGMIAAHRKMNESDIMHMNNMSRSGISTPQIHNTF----ASQTG 163
           K++ DEH +   DD     +         D +   N+  + +S P   N F     S T 
Sbjct: 296 KVYEDEHPN---DDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTD 352

Query: 164 GYQKVRFSKGNMYNVQGRQRRMKKKEPEKTDAEFAISYIRGLGSKDPLLYCRHVANDDGN 223
           GY   +FS G  Y VQ     +  K+ +    +   SY     S +P L   +  ND G 
Sbjct: 353 GYATQKFSVG--YAVQNFNATVSTKQFQVFSEQNTSSY-----SAEPQLDVNYYQNDVGP 405

Query: 224 LDRLFWSDGISQLN 237
            D   +   +  +N
Sbjct: 406 FDTRIYGQAVHFVN 419


>OSTA_ECOL6 (Q8CWE6) Organic solvent tolerance protein precursor
          Length = 784

 Score = 32.7 bits (73), Expect = 2.7
 Identities = 33/134 (24%), Positives = 51/134 (37%), Gaps = 14/134 (10%)

Query: 108 KLFSDEHNHELEDDKMCGMIAAHRKMNESDIMHMNNMSRSGISTPQIHNTF----ASQTG 163
           K++ DEH +   DD     +         D +   N+  + +S P   N F     S T 
Sbjct: 296 KVYKDEHPN---DDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTD 352

Query: 164 GYQKVRFSKGNMYNVQGRQRRMKKKEPEKTDAEFAISYIRGLGSKDPLLYCRHVANDDGN 223
           GY   +FS G  Y VQ     +  K+ +    +   SY     S +P L   +  ND G 
Sbjct: 353 GYATQKFSVG--YAVQNFNATVSTKQFQVFSEQNTSSY-----SAEPQLDVNYYQNDVGP 405

Query: 224 LDRLFWSDGISQLN 237
            D   +   +  +N
Sbjct: 406 FDTRIYGQAVHFVN 419


>OSTA_ECO57 (Q8XA13) Organic solvent tolerance protein precursor
          Length = 784

 Score = 32.7 bits (73), Expect = 2.7
 Identities = 33/134 (24%), Positives = 51/134 (37%), Gaps = 14/134 (10%)

Query: 108 KLFSDEHNHELEDDKMCGMIAAHRKMNESDIMHMNNMSRSGISTPQIHNTF----ASQTG 163
           K++ DEH +   DD     +         D +   N+  + +S P   N F     S T 
Sbjct: 296 KVYEDEHPN---DDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTD 352

Query: 164 GYQKVRFSKGNMYNVQGRQRRMKKKEPEKTDAEFAISYIRGLGSKDPLLYCRHVANDDGN 223
           GY   +FS G  Y VQ     +  K+ +    +   SY     S +P L   +  ND G 
Sbjct: 353 GYATQKFSVG--YAVQNFNATVSTKQFQVFSEQNTSSY-----SAEPQLDVNYYQNDVGP 405

Query: 224 LDRLFWSDGISQLN 237
            D   +   +  +N
Sbjct: 406 FDTRIYGQAVHFVN 419


>TRA6_STAAU (P19775) Transposase for insertion sequence element
           IS256 in transposon Tn4001
          Length = 390

 Score = 31.6 bits (70), Expect = 5.9
 Identities = 27/109 (24%), Positives = 44/109 (39%), Gaps = 6/109 (5%)

Query: 233 ISQLNYQVFGDVVAFDATYGKNRY---KCPFVVFYGVNHHNKSTIFSTALVSNEKIETYV 289
           +S+ NY      V +     +NR     C   +  G+       I    + S E  ET+ 
Sbjct: 156 LSEKNYPYLMTDVLYIKVREENRVLSKSCHIAI--GITKDGDREIIGFMIQSGESEETWT 213

Query: 290 WLLERFLEAMKGKAPLFVITDGDKAMKAAIKQVFPNAHHRLCAWHILDN 338
              E +L+    +    VI+D  K + +AI++ F N   + C  H L N
Sbjct: 214 TFFE-YLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRN 261


>TRA6_ENTFA (P59787) Transposase for insertion sequence element
           IS256 in transposon Tn4001
          Length = 390

 Score = 31.6 bits (70), Expect = 5.9
 Identities = 27/109 (24%), Positives = 44/109 (39%), Gaps = 6/109 (5%)

Query: 233 ISQLNYQVFGDVVAFDATYGKNRY---KCPFVVFYGVNHHNKSTIFSTALVSNEKIETYV 289
           +S+ NY      V +     +NR     C   +  G+       I    + S E  ET+ 
Sbjct: 156 LSEKNYPYLMTDVLYIKVREENRVLSKSCHIAI--GITKDGDREIIGFMIQSGESEETWT 213

Query: 290 WLLERFLEAMKGKAPLFVITDGDKAMKAAIKQVFPNAHHRLCAWHILDN 338
              E +L+    +    VI+D  K + +AI++ F N   + C  H L N
Sbjct: 214 TFFE-YLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRN 261


>2ABA_CANTR (P53031) Protein phosphatase PP2A regulatory subunit B
           (PR55) (Cell division control protein 55)
          Length = 508

 Score = 31.2 bits (69), Expect = 7.7
 Identities = 20/82 (24%), Positives = 33/82 (39%), Gaps = 10/82 (12%)

Query: 220 DDGNLDRLFWSDGISQLNYQVFGD----------VVAFDATYGKNRYKCPFVVFYGVNHH 269
           D G+++ +  +D IS + +   GD          VV F+    K +  C +  F     H
Sbjct: 11  DKGDIENITEADIISTVEFDHTGDFLATGDKGGRVVLFERNQSKKKQSCEYKFFTEFQSH 70

Query: 270 NKSTIFSTALVSNEKIETYVWL 291
           +    +  +L   EKI    WL
Sbjct: 71  DAEFDYLKSLEIEEKINKIKWL 92


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.340    0.149    0.499 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,426,147
Number of Sequences: 164201
Number of extensions: 2629545
Number of successful extensions: 7384
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 7381
Number of HSP's gapped (non-prelim): 11
length of query: 578
length of database: 59,974,054
effective HSP length: 116
effective length of query: 462
effective length of database: 40,926,738
effective search space: 18908152956
effective search space used: 18908152956
T: 11
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 69 (31.2 bits)