BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0302a.10
(243 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RPB1_PLAFD (P14248) DNA-directed RNA polymerase II largest subun... 35 0.13
SUV7_ARATH (Q9C5P1) Histone-lysine N-methyltransferase, H3 lysin... 34 0.36
AMRP_RAT (Q99068) Alpha-2-macroglobulin receptor-associated prot... 32 1.1
MCE1_KLULA (Q6CWR0) mRNA capping enzyme alpha subunit (mRNA guan... 32 1.8
CCR4_YEAST (P31384) Glucose-repressible alcohol dehydrogenase tr... 32 1.8
ASNA_MYCPE (Q8EWQ5) Aspartate--ammonia ligase (EC 6.3.1.1) (Aspa... 32 1.8
KEL1_YEAST (P38853) Kelch repeats protein 1 31 2.4
C5P3_DROME (Q95SK3) CDK5RAP3-like protein 30 4.0
ASNA_LACDE (P54264) Aspartate--ammonia ligase (EC 6.3.1.1) (Aspa... 30 4.0
ASNA_CLOPE (Q8XIY5) Aspartate--ammonia ligase (EC 6.3.1.1) (Aspa... 30 5.3
UAY_EMENI (P49413) Positive regulator of purine utilization 30 6.9
SMC4_SCHPO (P41004) Structural maintenance of chromosome 4 (Chro... 30 6.9
SAB2_MOUSE (Q8VI24) DNA-binding protein SATB2 (Special AT-rich s... 30 6.9
SAB2_HUMAN (Q9UPW6) DNA-binding protein SATB2 (Special AT-rich s... 30 6.9
LMO7_HUMAN (Q8WWI1) LIM domain only protein 7 (LOMP) (F-box only... 30 6.9
KNOB_PLAFN (P06719) Knob-associated histidine-rich protein precu... 30 6.9
KNOB_PLAFG (P09346) Knob-associated histidine-rich protein precu... 30 6.9
KNOB_PLAFD (P05229) Knob-associated histidine-rich protein (KAHR... 30 6.9
KNOB_PLAFA (P13817) Knob-associated histidine-rich protein precu... 30 6.9
KAPR_CANAL (Q9HEW1) cAMP-dependent protein kinase regulatory sub... 30 6.9
>RPB1_PLAFD (P14248) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6)
Length = 2452
Score = 35.4 bits (80), Expect = 0.13
Identities = 38/146 (26%), Positives = 60/146 (41%), Gaps = 22/146 (15%)
Query: 101 YISCNCWVQDTFDIDQTEHNKE--VILNTAKRLYGSHRGKLHEHFKKYETNEMALEHKPD 158
YI + + D DI T+ N E V+ K L G + +F Y+ + A E +
Sbjct: 1553 YIIYSVFSSDELDIIYTDDNSEDLVLRIRVKYLNGEY------NFMNYDVVDNANEQVDE 1606
Query: 159 EISEEDWEHLV-------DKFSSPTYKEMSARNKA-----NKSKQVINHRCGRKSFQAVS 206
+ EED EHLV D+ + T+ N +K K I+ K+ ++S
Sbjct: 1607 Q--EEDEEHLVANDRGNYDETKNSTHPHHDYNNNTTNIFKSKVKNNISSDINTKNEDSIS 1664
Query: 207 YDARDPETQKEPNYQDLWRMTHTNNN 232
++ + E K N + H NNN
Sbjct: 1665 INSSNNEQVKNINSSPVSNNMHNNNN 1690
>SUV7_ARATH (Q9C5P1) Histone-lysine N-methyltransferase, H3 lysine-9
specific 7 (EC 2.1.1.43) (Histone H3-K9
methyltransferase 7) (H3-K9-HMTase 7) (Suppressor of
variegation 3-9 homolog 7) (Su(var)3-9 homolog 7)
Length = 693
Score = 33.9 bits (76), Expect = 0.36
Identities = 17/57 (29%), Positives = 31/57 (53%), Gaps = 2/57 (3%)
Query: 155 HKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQVINHRCGRKSF-QAVSYDAR 210
++P+ + E+ WE + + + PT +SA+ K N + +NH C F Q + Y+ R
Sbjct: 579 YEPELLLEDSWEQVSEFINLPTQVLISAKEKGNVGR-FMNHSCSPNVFWQPIEYENR 634
>AMRP_RAT (Q99068) Alpha-2-macroglobulin receptor-associated protein
precursor (Alpha-2-MRAP) (Low density lipoprotein
receptor-related protein-associated protein 1) (RAP)
(GP330-binding 45 kDa protein) (Fragment)
Length = 357
Score = 32.3 bits (72), Expect = 1.1
Identities = 28/109 (25%), Positives = 48/109 (43%), Gaps = 9/109 (8%)
Query: 135 HRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQVIN 194
++ K+HE+ +T A E + +S D H+ + + E+ R ++ IN
Sbjct: 179 YKEKIHEYNVLLDTLSRAEEGYENLLSPSDMTHIKSDTLASKHSELKDRLRS------IN 232
Query: 195 HRCGRKSFQAVSYDARDPETQ-KEPNYQDLWRMTHTNNNGEWVNEASRE 242
G + VS+ P T+ +EP DLW + + N E E+ RE
Sbjct: 233 Q--GLDRLRKVSHQGYGPATEFEEPRVIDLWDLAQSANFTEKELESFRE 279
>MCE1_KLULA (Q6CWR0) mRNA capping enzyme alpha subunit (mRNA
guanylyltransferase) (EC 2.7.7.50) (GTP--RNA
guanylyltransferase) (GTase)
Length = 466
Score = 31.6 bits (70), Expect = 1.8
Identities = 22/73 (30%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
Query: 150 EMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQVINHRCGRKSFQAVSYDA 209
+ LE D +S +D E +VD+ S +KE K N KQ NH+ +S + +
Sbjct: 379 QKVLESIGDSVSLDDLEQVVDEMRS-RWKEREQGLK-NAQKQ-FNHQASARSSLSQQHST 435
Query: 210 RDPETQKEPNYQD 222
++Q +P Y D
Sbjct: 436 EPEQSQDQPKYVD 448
>CCR4_YEAST (P31384) Glucose-repressible alcohol dehydrogenase
transcriptional effector (EC 3.1.-.-) (Cytoplasmic
deadenylase) (Carbon catabolite repressor protein 4)
Length = 837
Score = 31.6 bits (70), Expect = 1.8
Identities = 20/81 (24%), Positives = 39/81 (47%), Gaps = 5/81 (6%)
Query: 136 RGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDK--FSSPTYKEMSARNKANKSKQVI 193
R KL E Y+++ + L+ + EE W L+DK ++ + + A+ +K + +
Sbjct: 537 RNKLKEQLLSYDSDLLCLQEVESKTFEEYWVPLLDKHGYTGIFHAKARAKTMHSKDSKKV 596
Query: 194 NHRC---GRKSFQAVSYDARD 211
+ C R F+ ++ DA D
Sbjct: 597 DGCCIFFKRDQFKLITKDAMD 617
>ASNA_MYCPE (Q8EWQ5) Aspartate--ammonia ligase (EC 6.3.1.1)
(Asparagine synthetase A)
Length = 326
Score = 31.6 bits (70), Expect = 1.8
Identities = 31/100 (31%), Positives = 44/100 (44%), Gaps = 3/100 (3%)
Query: 114 IDQTEHNKEVILNTAKRLYGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFS 173
I +TE N E + K++Y R E KKYE A P+EI+ E L+ ++
Sbjct: 122 IKKTERNLETLKVVVKKIYEIIRLCQKEVNKKYEW--FAENLLPEEITFISSEDLLQRYP 179
Query: 174 SPTYKEMSARNKANKSKQVINHRCGRKSFQAVSYDARDPE 213
+ T KE R A+K K V G +D R P+
Sbjct: 180 NKTPKERE-RLIASKYKAVFIIGIGDNLSDGKPHDLRAPD 218
>KEL1_YEAST (P38853) Kelch repeats protein 1
Length = 1164
Score = 31.2 bits (69), Expect = 2.4
Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
Query: 115 DQTEHNKEVILNTAKRLYGSHRGKLHEHFKKYETNEMALEHKPDEISEED 164
+ +HN+++I N A Y S G L H+K+ N LE IS D
Sbjct: 914 ESKQHNEDIINNVAN--YSSQLGSLISHWKENRANSSFLESSSSLISVSD 961
>C5P3_DROME (Q95SK3) CDK5RAP3-like protein
Length = 509
Score = 30.4 bits (67), Expect = 4.0
Identities = 18/79 (22%), Positives = 35/79 (43%)
Query: 151 MALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQVINHRCGRKSFQAVSYDAR 210
+++E + S++ +HL SP Y M A +K V R R++ + ++ + R
Sbjct: 406 VSVEKIIQQTSDKQTQHLFQLKHSPKYANMLATKLQQMTKAVEKLRATREALKQLTIELR 465
Query: 211 DPETQKEPNYQDLWRMTHT 229
+ P ++L T T
Sbjct: 466 EQRQDLNPVLEELIAQTRT 484
>ASNA_LACDE (P54264) Aspartate--ammonia ligase (EC 6.3.1.1)
(Asparagine synthetase A)
Length = 336
Score = 30.4 bits (67), Expect = 4.0
Identities = 24/100 (24%), Positives = 41/100 (41%), Gaps = 3/100 (3%)
Query: 114 IDQTEHNKEVILNTAKRLYGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFS 173
ID++E + + T +R++ + +G + Y A H P+EIS E L ++
Sbjct: 133 IDKSERTEATLRQTVQRIFETIKGMEYHVRALYP---QAAYHLPEEISFVTSEELEARWP 189
Query: 174 SPTYKEMSARNKANKSKQVINHRCGRKSFQAVSYDARDPE 213
S T E + K + H G +D R P+
Sbjct: 190 SLTPSEREDKICQEKGAVFLEHIGGALPLSKKPHDLRAPD 229
>ASNA_CLOPE (Q8XIY5) Aspartate--ammonia ligase (EC 6.3.1.1)
(Asparagine synthetase A)
Length = 336
Score = 30.0 bits (66), Expect = 5.3
Identities = 20/66 (30%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Query: 114 IDQTEHNKEVILNTAKRLYGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFS 173
ID+ E N E + T K +YG+ + KY E L P++I+ + L D++
Sbjct: 133 IDKEERNLETLKETVKSIYGTFKATEDFIVAKYPHIEKIL---PEDITFITSQELEDRYP 189
Query: 174 SPTYKE 179
T KE
Sbjct: 190 DLTSKE 195
>UAY_EMENI (P49413) Positive regulator of purine utilization
Length = 1060
Score = 29.6 bits (65), Expect = 6.9
Identities = 17/72 (23%), Positives = 36/72 (49%), Gaps = 1/72 (1%)
Query: 162 EEDWEHLVDKFSSPTYKEMSARNKAN-KSKQVINHRCGRKSFQAVSYDARDPETQKEPNY 220
E +E + P E++A++ +N KS ++ + G + S DPE +++ +
Sbjct: 146 EAGYEAYAGSANGPAAGEIAAQDGSNDKSVRIKKEKNGPLGLEKASRYDHDPEVKQDSDA 205
Query: 221 QDLWRMTHTNNN 232
+D WR+ + +N
Sbjct: 206 EDGWRIQNLVSN 217
>SMC4_SCHPO (P41004) Structural maintenance of chromosome 4
(Chromosome segregation protein cut3)
Length = 1324
Score = 29.6 bits (65), Expect = 6.9
Identities = 21/105 (20%), Positives = 49/105 (46%), Gaps = 11/105 (10%)
Query: 116 QTEHNKEVILNTAKRLYGSHRGKLHEHFKKYETNEMALEHKPDEI-------SEEDWEHL 168
+T + K ++ N L S GKL H +K+E E + K +E+ ++ +
Sbjct: 367 ETRNKKTLVQN----LLNSLEGKLQAHLEKFEQTERDISEKNEEVKSLREKAAKVKNDCT 422
Query: 169 VDKFSSPTYKEMSARNKANKSKQVINHRCGRKSFQAVSYDARDPE 213
+K + +Y++ + + + + + +KS +A+S++ + E
Sbjct: 423 SEKKTRQSYEQQTVKIEEQLKFLLNKEKKLKKSIEALSFEKSEAE 467
>SAB2_MOUSE (Q8VI24) DNA-binding protein SATB2 (Special AT-rich
sequence-binding protein 2)
Length = 733
Score = 29.6 bits (65), Expect = 6.9
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Query: 129 KRLYGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANK 188
+R + H GKL EH E+K +E+ E E+ ++ S YK + A+K
Sbjct: 666 QRYHVKHHGKLKEHLGSAVD---VAEYKDEELLTESEENDSEEGSEEMYKVEAEEENADK 722
Query: 189 SK 190
SK
Sbjct: 723 SK 724
>SAB2_HUMAN (Q9UPW6) DNA-binding protein SATB2 (Special AT-rich
sequence-binding protein 2)
Length = 733
Score = 29.6 bits (65), Expect = 6.9
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Query: 129 KRLYGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANK 188
+R + H GKL EH E+K +E+ E E+ ++ S YK + A+K
Sbjct: 666 QRYHVKHHGKLKEHLGSAVD---VAEYKDEELLTESEENDSEEGSEEMYKVEAEEENADK 722
Query: 189 SK 190
SK
Sbjct: 723 SK 724
>LMO7_HUMAN (Q8WWI1) LIM domain only protein 7 (LOMP) (F-box only
protein 20)
Length = 1683
Score = 29.6 bits (65), Expect = 6.9
Identities = 23/86 (26%), Positives = 38/86 (43%), Gaps = 4/86 (4%)
Query: 138 KLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQVINHRC 197
K + K Y ++ + + +EI ++ E K SS T+KEM ++ K + R
Sbjct: 744 KWKDRRKSYTSDLQKKKEEREEIEKQALEK--SKRSSKTFKEMLQDRESQNQKSTVPSRR 801
Query: 198 GRKSFQAVSYDARDPETQ--KEPNYQ 221
SF V + + P T E +YQ
Sbjct: 802 RMYSFDDVLEEGKRPPTMTVSEASYQ 827
>KNOB_PLAFN (P06719) Knob-associated histidine-rich protein
precursor (KAHRP)
Length = 657
Score = 29.6 bits (65), Expect = 6.9
Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 4/85 (4%)
Query: 132 YGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQ 191
YG H + H KK+E N+ E K + S++ +H +K S +K+ + ++ KSK+
Sbjct: 355 YGKHGDEKHHSSKKHEGNDGEGEKK--KKSKKHKDHDGEKKKSKKHKD-NEDAESVKSKK 411
Query: 192 VINHRCGRKSFQAVSYDARDPETQK 216
+H C +K + D D E+ K
Sbjct: 412 HKSHDCEKKKSKK-HKDNEDAESVK 435
>KNOB_PLAFG (P09346) Knob-associated histidine-rich protein
precursor (KAHRP) (KP)
Length = 634
Score = 29.6 bits (65), Expect = 6.9
Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 4/85 (4%)
Query: 132 YGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQ 191
YG H + H KK+E N+ E K + S++ +H +K S +K+ + ++ KSK+
Sbjct: 352 YGKHGDEKHHSSKKHEGNDGEGEKK--KKSKKHKDHDGEKKKSKKHKD-NEDAESVKSKK 408
Query: 192 VINHRCGRKSFQAVSYDARDPETQK 216
+H C +K + D D E+ K
Sbjct: 409 HKSHDCEKKKSKK-HKDNEDAESVK 432
>KNOB_PLAFD (P05229) Knob-associated histidine-rich protein (KAHRP)
(Fragment)
Length = 277
Score = 29.6 bits (65), Expect = 6.9
Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 4/85 (4%)
Query: 132 YGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQ 191
YG H + H KK+E N+ E K + S++ +H +K S +K+ + ++ KSK+
Sbjct: 167 YGKHGDEKHHSSKKHEGNDGEGEKK--KKSKKHKDHDGEKKKSKKHKD-NEDAESVKSKK 223
Query: 192 VINHRCGRKSFQAVSYDARDPETQK 216
+H C +K + D D E+ K
Sbjct: 224 HKSHDCEKKKSKK-HKDNEDAESVK 247
>KNOB_PLAFA (P13817) Knob-associated histidine-rich protein
precursor (KAHRP) (HRPI) (Fragment)
Length = 473
Score = 29.6 bits (65), Expect = 6.9
Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 4/85 (4%)
Query: 132 YGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQ 191
YG H + H KK+E N+ E K + S++ +H +K S +K+ + ++ KSK+
Sbjct: 352 YGKHGDEKHHSSKKHEGNDGEGEKK--KKSKKHKDHDGEKKKSKKHKD-NEDAESVKSKK 408
Query: 192 VINHRCGRKSFQAVSYDARDPETQK 216
+H C +K + D D E+ K
Sbjct: 409 HKSHDCEKKKSKK-HKDNEDAESVK 432
>KAPR_CANAL (Q9HEW1) cAMP-dependent protein kinase regulatory
subunit (PKA regulatory subunit) (PKA-R)
Length = 459
Score = 29.6 bits (65), Expect = 6.9
Identities = 27/102 (26%), Positives = 42/102 (40%), Gaps = 8/102 (7%)
Query: 115 DQTEHNKEVILNTAKRLYGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSS 174
D+ H K+ +TA G K + KK +N E PD+ S+ S
Sbjct: 99 DEDPHAKDTKTDTAAAAVGGGIFKSNFDVKKSASNPPTKEVDPDDPSKP------SSSSQ 152
Query: 175 PTYKEMSARNKANKSKQVINHRCGRKSFQAVSYDARDPETQK 216
P + SA +K SK + R++ +VS +A +P K
Sbjct: 153 PNQQSASASSKTPSSKIPVAFNANRRT--SVSAEALNPAKLK 192
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.133 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,290,277
Number of Sequences: 164201
Number of extensions: 1177744
Number of successful extensions: 3106
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 3103
Number of HSP's gapped (non-prelim): 26
length of query: 243
length of database: 59,974,054
effective HSP length: 107
effective length of query: 136
effective length of database: 42,404,547
effective search space: 5767018392
effective search space used: 5767018392
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0302a.10