BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0301c.1
(229 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ERF4_ARATH (O80340) Ethylene responsive element binding factor 4... 161 1e-39
ERF3_ARATH (O80339) Ethylene responsive element binding factor 3... 124 2e-28
AP23_ARATH (P42736) AP2 domain transcription factor RAP2.3 (Rela... 96 9e-20
PTI6_LYCES (O04682) Pathogenesis-related genes transcriptional a... 93 5e-19
ERF5_ARATH (O80341) Ethylene responsive element binding factor 5... 92 8e-19
ERF2_ARATH (O80338) Ethylene responsive element binding factor 2... 92 8e-19
ERF1_ARATH (O80337) Ethylene responsive element binding factor 1... 89 9e-18
DR2A_ARATH (O82132) Dehydration responsive element binding prote... 86 6e-17
PTI5_LYCES (O04681) Pathogenesis-related genes transcriptional a... 84 4e-16
DR2F_ARATH (Q9SVX5) Dehydration responsive element binding prote... 84 4e-16
DR2D_ARATH (Q9LQZ2) Putative dehydration responsive element bind... 84 4e-16
DR2B_ARATH (O82133) Dehydration responsive element binding prote... 82 1e-15
TINY_ARATH (Q39127) Transcriptional factor TINY 79 1e-14
DR2G_ARATH (P61827) Putative dehydration responsive element bind... 77 3e-14
DR2C_ARATH (Q8LFR2) Dehydration responsive element binding prote... 75 1e-13
PZ02_LUPPO (P16146) PPLZ02 protein 75 1e-13
DR2E_ARATH (O80917) Dehydration responsive element binding prote... 73 6e-13
DR1C_ARATH (Q9SYS6) Dehydration responsive element binding prote... 71 2e-12
DR2H_ARATH (Q9SIZ0) Putative dehydration responsive element bind... 71 2e-12
DR1B_ARATH (P93835) Dehydration responsive element binding prote... 70 3e-12
>ERF4_ARATH (O80340) Ethylene responsive element binding factor 4
(AtERF4)
Length = 222
Score = 161 bits (408), Expect = 1e-39
Identities = 103/217 (47%), Positives = 128/217 (58%), Gaps = 19/217 (8%)
Query: 21 NGGVKEVHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAAREFRGPKA 80
+ KE+ +RGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEEAARAYDTAAR+FRG KA
Sbjct: 17 HNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDFRGAKA 76
Query: 81 KTNFP----FPSDNVKN---QSPSQSSTVESSSRDRDVAAAADSSSLDLNLAPSVRFPFR 133
KTNFP V +SPSQSST++ +S V +A + ++ L S+
Sbjct: 77 KTNFPTFLELSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAGNVPPQLELSLGGGGG 136
Query: 134 PQFPQMPAANQVLFFDAILRAGMVG-GSPASQRYGFDFHTYNHPVAASEIQAVAGAQSDS 192
Q+P + V F D ++ G VG G P F PV + GAQSDS
Sbjct: 137 GSCYQIPMSRPVYFLD-LMGIGNVGRGQPPPVTSAF-----RSPVVHVATKMACGAQSDS 190
Query: 193 DSSSVIDLNHYESGVVIKAGGRNFDLDLNYPPMEDMA 229
DSSSV+D +E G ++ + DLDLN PP + A
Sbjct: 191 DSSSVVD---FEGG--MEKRSQLLDLDLNLPPPSEQA 222
>ERF3_ARATH (O80339) Ethylene responsive element binding factor 3
(AtERF3)
Length = 225
Score = 124 bits (310), Expect = 2e-28
Identities = 82/208 (39%), Positives = 112/208 (53%), Gaps = 22/208 (10%)
Query: 21 NGGVKEVHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAAREFRGPKA 80
NG VKE+ FRGVRKRPWGR+AAEIRDP KK+RVWLGTFD+AEEAARAYD+AAR RGPKA
Sbjct: 20 NGSVKEIRFRGVRKRPWGRFAAEIRDPWKKARVWLGTFDSAEEAARAYDSAARNLRGPKA 79
Query: 81 KTNFPFPSDNVKNQSPSQSSTVESSSRDRDVAAAADSSSLDLNLAPSVRFPFRPQFPQMP 140
KTNFP + + SP + + R++ + + +D + + + QFP +
Sbjct: 80 KTNFP-----IDSSSPPPPNLRFNQIRNQ------NQNQVDPFMDHRLFTDHQQQFPIVN 128
Query: 141 AANQVLFFDAILRAGMVGGSPASQRYGFDFHTYNHPVAASEIQAVAGAQSDSDSSSVIDL 200
+ G P + + T +P + + DS SSSVID
Sbjct: 129 RPTSSSMSSTV--ESFSGPRPTTMK---PATTKRYPRTPPVVPEDCHSDCDS-SSSVID- 181
Query: 201 NHYESGVVIKAGGRN--FDLDLNYPPME 226
+ + + RN F DLN+PP++
Sbjct: 182 --DDDDIASSSRRRNPPFQFDLNFPPLD 207
>AP23_ARATH (P42736) AP2 domain transcription factor RAP2.3 (Related
to AP2 protein 3) (Cadmium-induced protein AS30)
Length = 248
Score = 95.5 bits (236), Expect = 9e-20
Identities = 47/82 (57%), Positives = 58/82 (70%), Gaps = 4/82 (4%)
Query: 29 FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAAREFRGPKAKTNFPFPS 88
+RG+RKRPWG++AAEIRDP K RVWLGTF+TAEEAA AYD AA++ RG KAK NFP
Sbjct: 79 YRGIRKRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAAMAYDVAAKQIRGDKAKLNFP--- 135
Query: 89 DNVKNQSPSQSSTVESSSRDRD 110
++ + P + SS R D
Sbjct: 136 -DLHHPPPPNYTPPPSSPRSTD 156
>PTI6_LYCES (O04682) Pathogenesis-related genes transcriptional
activator PTI6
Length = 248
Score = 93.2 bits (230), Expect = 5e-19
Identities = 47/99 (47%), Positives = 62/99 (62%), Gaps = 7/99 (7%)
Query: 29 FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAAREFRGPKAKTNFPFPS 88
FRGVR+RPWGR+AAEIRDP + RVWLGT+DT EEAA YD AA + +GP A TNFP
Sbjct: 98 FRGVRQRPWGRWAAEIRDPTRGKRVWLGTYDTPEEAAVVYDKAAVKLKGPDAVTNFPV-- 155
Query: 89 DNVKNQSPSQSSTVESSSRDRDVAAAADSSSLDLNLAPS 127
+ ++ + + + VA D S D+ L+P+
Sbjct: 156 -----STTAEVTVTVTETETESVADGGDKSENDVALSPT 189
>ERF5_ARATH (O80341) Ethylene responsive element binding factor 5
(AtERF5)
Length = 300
Score = 92.4 bits (228), Expect = 8e-19
Identities = 49/84 (58%), Positives = 55/84 (65%), Gaps = 4/84 (4%)
Query: 28 HFRGVRKRPWGRYAAEIRDPGKK-SRVWLGTFDTAEEAARAYDTAAREFRGPKAKTNFPF 86
H+RGVR+RPWG++AAEIRDP K+ SRVWLGTFDTA EAARAYD AA RG KA NFP
Sbjct: 155 HYRGVRQRPWGKFAAEIRDPNKRGSRVWLGTFDTAIEAARAYDEAAFRLRGSKAILNFPL 214
Query: 87 PSDNVKNQSPSQSSTVESSSRDRD 110
V P + RD D
Sbjct: 215 ---EVGKWKPRADEGEKKRKRDDD 235
>ERF2_ARATH (O80338) Ethylene responsive element binding factor 2
(AtERF2)
Length = 243
Score = 92.4 bits (228), Expect = 8e-19
Identities = 50/89 (56%), Positives = 60/89 (67%), Gaps = 6/89 (6%)
Query: 24 VKEVHFRGVRKRPWGRYAAEIRDPGKK-SRVWLGTFDTAEEAARAYDTAAREFRGPKAKT 82
VK H+RGVR+RPWG++AAEIRDP K +RVWLGTF+TAE+AA AYD AA RG +A
Sbjct: 112 VKAKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDIAAFRMRGSRALL 171
Query: 83 NFPF-----PSDNVKNQSPSQSSTVESSS 106
NFP D V+ S SS+ SSS
Sbjct: 172 NFPLRVNSGEPDPVRITSKRSSSSSSSSS 200
>ERF1_ARATH (O80337) Ethylene responsive element binding factor 1
(AtERF1) (EREBP-2 protein)
Length = 268
Score = 89.0 bits (219), Expect = 9e-18
Identities = 50/99 (50%), Positives = 67/99 (67%), Gaps = 10/99 (10%)
Query: 25 KEVHFRGVRKRPWGRYAAEIRDPGKK-SRVWLGTFDTAEEAARAYDTAAREFRGPKAKTN 83
K H+RGVR+RPWG++AAEIRDP K +RVWLGTF+TAE+AA AYD AA RG +A N
Sbjct: 144 KGKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDRAAFRMRGSRALLN 203
Query: 84 FPFPSDN-------VKNQSPSQSSTVESSS--RDRDVAA 113
FP ++ +K++ S SS+ E+ + + R VAA
Sbjct: 204 FPLRVNSGEPDPVRIKSKRSSFSSSNENGAPKKRRTVAA 242
>DR2A_ARATH (O82132) Dehydration responsive element binding protein
2A (DREB2A protein)
Length = 335
Score = 86.3 bits (212), Expect = 6e-17
Identities = 48/98 (48%), Positives = 61/98 (61%), Gaps = 4/98 (4%)
Query: 11 KVSGNGNGNGNGGVKEVH--FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAY 68
K S G G GG + FRGVR+R WG++ AEIR+P + SR+WLGTF TA+EAA AY
Sbjct: 59 KGSKKGCMKGKGGPENSRCSFRGVRQRIWGKWVAEIREPNRGSRLWLGTFPTAQEAASAY 118
Query: 69 DTAAREFRGPKAKTNFP--FPSDNVKNQSPSQSSTVES 104
D AA+ GP A+ NFP S+ S S+ TVE+
Sbjct: 119 DEAAKAMYGPLARLNFPRSDASEVTSTSSQSEVCTVET 156
>PTI5_LYCES (O04681) Pathogenesis-related genes transcriptional
activator PTI5
Length = 161
Score = 83.6 bits (205), Expect = 4e-16
Identities = 49/98 (50%), Positives = 58/98 (59%), Gaps = 15/98 (15%)
Query: 29 FRGVRKRPWGRYAAEIRDPGKK-SRVWLGTFDTAEEAARAYDTAAREFRGPKAKTNFP-- 85
+RGVR+RPWG+YAAEIRD + +RVWLGTF+TAEEAA AYD AA RG KA NFP
Sbjct: 59 YRGVRRRPWGKYAAEIRDSARHGARVWLGTFETAEEAALAYDRAAFRMRGAKALLNFPSE 118
Query: 86 ------------FPSDNVKNQSPSQSSTVESSSRDRDV 111
S++ + S SS E SS DV
Sbjct: 119 IVNASVSVDKLSLCSNSYTTNNNSDSSLNEVSSGTNDV 156
>DR2F_ARATH (Q9SVX5) Dehydration responsive element binding protein
2F (DREB2F protein)
Length = 277
Score = 83.6 bits (205), Expect = 4e-16
Identities = 48/126 (38%), Positives = 71/126 (56%), Gaps = 3/126 (2%)
Query: 5 DKTNAVKVSGNGNGNGNGGVKEV--HFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAE 62
+K++++K G G GG + +RGVR+R WG++ AEIR+P K++R+WLG+F TAE
Sbjct: 2 EKSSSMKQWKKGPARGKGGPQNALCQYRGVRQRTWGKWVAEIREPKKRARLWLGSFATAE 61
Query: 63 EAARAYDTAAREFRGPKAKTNFPFPSDNVKNQSPSQSSTVESSSRDRDVAAAADSSSLDL 122
EAA AYD AA + G A N P N + S S S + + ++ L++
Sbjct: 62 EAAMAYDEAALKLYGHDAYLNLPHLQRNTR-PSLSNSQRFKWVPSRKFISMFPSCGMLNV 120
Query: 123 NLAPSV 128
N PSV
Sbjct: 121 NAQPSV 126
>DR2D_ARATH (Q9LQZ2) Putative dehydration responsive element binding
protein 2D (DREB2D protein)
Length = 206
Score = 83.6 bits (205), Expect = 4e-16
Identities = 47/117 (40%), Positives = 68/117 (57%), Gaps = 10/117 (8%)
Query: 13 SGNGNGNGNGGVKEVH--FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDT 70
S G G GG ++GVR+R WG++ AEIR+P + +R+WLGTFDT+ EAA AYD+
Sbjct: 24 SRKGCMRGKGGPDNASCTYKGVRQRTWGKWVAEIREPNRGARLWLGTFDTSREAALAYDS 83
Query: 71 AAREFRGPKAKTNFP-----FP---SDNVKNQSPSQSSTVESSSRDRDVAAAADSSS 119
AAR+ GP+A N P +P S +PS ++ +SSS ++ + SS
Sbjct: 84 AARKLYGPEAHLNLPESLRSYPKTASSPASQTTPSSNTGGKSSSDSESPCSSNEMSS 140
>DR2B_ARATH (O82133) Dehydration responsive element binding protein
2B (DREB2B protein)
Length = 330
Score = 81.6 bits (200), Expect = 1e-15
Identities = 50/108 (46%), Positives = 63/108 (58%), Gaps = 5/108 (4%)
Query: 3 PRDKTNAVKVSGNGNGNGNGGVKEVH--FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDT 60
P+ K A K S G G GG H FRGVR+R WG++ AEIR+P +R+WLGTF T
Sbjct: 51 PKRKVPA-KGSKKGCMKGKGGPDNSHCSFRGVRQRIWGKWVAEIREPKIGTRLWLGTFPT 109
Query: 61 AEEAARAYDTAAREFRGPKAKTNFP--FPSDNVKNQSPSQSSTVESSS 106
AE+AA AYD AA G A+ NFP S+ S S+ TVE+ +
Sbjct: 110 AEKAASAYDEAATAMYGSLARLNFPQSVGSEFTSTSSQSEVCTVENKA 157
>TINY_ARATH (Q39127) Transcriptional factor TINY
Length = 218
Score = 78.6 bits (192), Expect = 1e-14
Identities = 43/85 (50%), Positives = 54/85 (62%), Gaps = 7/85 (8%)
Query: 29 FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAAREFRGPKAKTNFPFPS 88
+RGVRKR WG++ +EIR+P KKSR+WLGTF + E AARA+D AA +G A NFP
Sbjct: 36 YRGVRKRNWGKWVSEIREPRKKSRIWLGTFPSPEMAARAHDVAALSIKGASAILNFP--- 92
Query: 89 DNVKNQSPSQSSTVESSSRDRDVAA 113
++ P SS S RD VAA
Sbjct: 93 -DLAGSFPRPSSL---SPRDIQVAA 113
>DR2G_ARATH (P61827) Putative dehydration responsive element
binding protein 2G (DREB2G protein)
Length = 307
Score = 77.0 bits (188), Expect = 3e-14
Identities = 41/87 (47%), Positives = 55/87 (63%), Gaps = 3/87 (3%)
Query: 3 PRDKTNAVKVSGNGNGNGNGGVKEVH--FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDT 60
P K N + S G G GG + FRGVR+R WG++ AEIR+P + +R+WLGTF+T
Sbjct: 7 PAKKRNMGR-SRKGCMKGKGGPENATCTFRGVRQRTWGKWVAEIREPNRGTRLWLGTFNT 65
Query: 61 AEEAARAYDTAAREFRGPKAKTNFPFP 87
+ EAA AYD AA++ G +AK N P
Sbjct: 66 SVEAAMAYDEAAKKLYGHEAKLNLVHP 92
>DR2C_ARATH (Q8LFR2) Dehydration responsive element binding protein
2C (DREB2C protein)
Length = 341
Score = 75.5 bits (184), Expect = 1e-13
Identities = 45/117 (38%), Positives = 67/117 (56%), Gaps = 12/117 (10%)
Query: 11 KVSGNGNGNGNGGVKE--VHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAY 68
K S G G GG + +RGVR+R WG++ AEIR+P +R+WLGTF ++ EAA AY
Sbjct: 52 KGSRKGCMKGKGGPENGICDYRGVRQRRWGKWVAEIREPDGGARLWLGTFSSSYEAALAY 111
Query: 69 DTAAREFRGPKAKTNFPFPSDNVKNQSPSQSST------VESSSRDRDVAAAADSSS 119
D AA+ G A+ N P + N+S S ++T V + S + +V A D+++
Sbjct: 112 DEAAKAIYGQSARLNLP----EITNRSSSTAATATVSGSVTAFSDESEVCAREDTNA 164
>PZ02_LUPPO (P16146) PPLZ02 protein
Length = 164
Score = 75.1 bits (183), Expect = 1e-13
Identities = 37/73 (50%), Positives = 51/73 (69%), Gaps = 6/73 (8%)
Query: 29 FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAAREFRGPKAKTNFPFPS 88
+RG R+R WG + +EIR K+R+W GTF++AE+AARAYD AAR G +A+TNFP+
Sbjct: 8 YRGFRQRHWGSWVSEIRHSILKTRIWQGTFESAEDAARAYDEAARLMCGTRARTNFPY-- 65
Query: 89 DNVKNQSPSQSST 101
N + SQSS+
Sbjct: 66 ----NPNASQSSS 74
>DR2E_ARATH (O80917) Dehydration responsive element binding protein
2E (DREB2E protein)
Length = 244
Score = 72.8 bits (177), Expect = 6e-13
Identities = 41/85 (48%), Positives = 49/85 (57%), Gaps = 10/85 (11%)
Query: 11 KVSGNGNGNGNGGVKE--VHFRGVRKRPWGRYAAEIRDP--------GKKSRVWLGTFDT 60
K S G G GG + FRGVR+R WG++ AEIR+P + R+WLGTF T
Sbjct: 50 KGSKKGCMRGKGGPENPVCRFRGVRQRVWGKWVAEIREPVSHRGANSSRSKRLWLGTFAT 109
Query: 61 AEEAARAYDTAAREFRGPKAKTNFP 85
A EAA AYD AA GP A+ NFP
Sbjct: 110 AAEAALAYDRAASVMYGPYARLNFP 134
>DR1C_ARATH (Q9SYS6) Dehydration responsive element binding protein
1C (DREB1C protein) (C-repeat binding factor 2)
(C-repeat/dehydration responsive element binding factor
2) (CRT/DRE binding factor 2)
Length = 216
Score = 71.2 bits (173), Expect = 2e-12
Identities = 50/154 (32%), Positives = 74/154 (47%), Gaps = 13/154 (8%)
Query: 29 FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAAREFRGPKAKTNFP--- 85
+RGVR+R G++ E+R+P KK+R+WLGTF TAE AARA+D AA RG A NF
Sbjct: 51 YRGVRQRNSGKWVCELREPNKKTRIWLGTFQTAEMAARAHDVAAIALRGRSACLNFADSA 110
Query: 86 ----FPSDNVKNQSPSQSSTVESSSRDRDVAAAADSSSLDL--NLAPSVRFPFRPQFPQM 139
P + ++ + +D D+ LD+ L ++ P + Q
Sbjct: 111 WRLRIPESTCAKEIQKAAAEAALNFQDEMCHMTTDAHGLDMEETLVEAIYTPEQSQDAFY 170
Query: 140 PAANQVLFFDAIL---RAGMVGGSPASQ-RYGFD 169
+L ++L GM+ SP+ Q Y FD
Sbjct: 171 MDEEAMLGMSSLLDNMAEGMLLPSPSVQWNYNFD 204
>DR2H_ARATH (Q9SIZ0) Putative dehydration responsive element binding
protein 2H (DREB2H protein)
Length = 164
Score = 70.9 bits (172), Expect = 2e-12
Identities = 34/77 (44%), Positives = 49/77 (63%), Gaps = 2/77 (2%)
Query: 11 KVSGNGNGNGNGGVKE--VHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAY 68
K S G G GG + + GVR+R WG++ AEIR+PG+ +++WLGTF ++ EAA AY
Sbjct: 54 KRSRKGCMKGKGGPENGICDYTGVRQRTWGKWVAEIREPGRGAKLWLGTFSSSYEAALAY 113
Query: 69 DTAAREFRGPKAKTNFP 85
D A++ G A+ N P
Sbjct: 114 DEASKAIYGQSARLNLP 130
>DR1B_ARATH (P93835) Dehydration responsive element binding protein
1B (DREB1B protein) (C-repeat binding factor 1)
(C-repeat/dehydration responsive element binding factor
1) (CRT/DRE binding factor 1)
Length = 213
Score = 70.5 bits (171), Expect = 3e-12
Identities = 32/56 (57%), Positives = 42/56 (74%)
Query: 29 FRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAAREFRGPKAKTNF 84
+RGVR+R G++ +E+R+P KK+R+WLGTF TAE AARA+D AA RG A NF
Sbjct: 48 YRGVRQRNSGKWVSEVREPNKKTRIWLGTFQTAEMAARAHDVAALALRGRSACLNF 103
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.314 0.131 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,067,290
Number of Sequences: 164201
Number of extensions: 1161680
Number of successful extensions: 2856
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 35
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 2803
Number of HSP's gapped (non-prelim): 53
length of query: 229
length of database: 59,974,054
effective HSP length: 107
effective length of query: 122
effective length of database: 42,404,547
effective search space: 5173354734
effective search space used: 5173354734
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0301c.1