BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0299.12
(418 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TRIP_MOUSE (Q8VIG6) TRAF-interacting protein 42 0.003
TRIP_HUMAN (Q9BWF2) TRAF-interacting protein 42 0.003
S24A_HUMAN (O95486) Protein transport protein Sec24A (SEC24-rela... 40 0.015
RH3A_ARATH (O22755) RING-H2 zinc finger protein RHA3a 40 0.015
RNF4_MOUSE (Q9QZS2) RING finger protein 4 39 0.019
RNF4_RAT (O88846) RING finger protein 4 (SNURF) 39 0.032
RNF4_HUMAN (P78317) RING finger protein 4 39 0.032
RAPS_MOUSE (P12672) 43 kDa receptor-associated protein of the sy... 38 0.042
RAPS_HUMAN (Q13702) 43 kDa receptor-associated protein of the sy... 38 0.042
TM25_MOUSE (Q61510) Tripartite motif-containing protein 25 (Zinc... 38 0.055
NF7B_XENLA (Q92021) Nuclear factor 7, brain (xnf7) (xnf7-B) 37 0.072
TRM7_HUMAN (Q9C029) Tripartite motif-containing protein 7 (Glyco... 37 0.094
TM47_HUMAN (Q96LD4) Tripartite motif-containing protein 47 (RING... 37 0.094
RAPS_CHICK (O42393) 43 kDa receptor-associated protein of the sy... 37 0.094
TTC3_HUMAN (P53804) Tetratricopeptide repeat protein 3 (TPR repe... 37 0.12
BSN_RAT (O88778) Bassoon protein 37 0.12
YKD4_YEAST (P36096) Hypothetical 87.9 kDa protein in PTM-IRX1 in... 36 0.16
WETA_EMENI (P22022) Regulatory protein wetA 36 0.16
RH3B_ARATH (Q9ZT49) RING-H2 zinc finger protein RHA3b 36 0.16
RAPS_TORCA (P09108) 43 kDa receptor-associated protein of the sy... 36 0.16
>TRIP_MOUSE (Q8VIG6) TRAF-interacting protein
Length = 470
Score = 42.0 bits (97), Expect = 0.003
Identities = 18/46 (39%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
C+IC + + R A +HCGH FHL C+ F + CP CR
Sbjct: 7 CTICSDFF--DHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCR 50
>TRIP_HUMAN (Q9BWF2) TRAF-interacting protein
Length = 469
Score = 42.0 bits (97), Expect = 0.003
Identities = 18/46 (39%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
C+IC + + R A +HCGH FHL C+ F + CP CR
Sbjct: 7 CTICSDFF--DHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCR 50
>S24A_HUMAN (O95486) Protein transport protein Sec24A (SEC24-related
protein A) (Fragment)
Length = 1078
Score = 39.7 bits (91), Expect = 0.015
Identities = 37/133 (27%), Positives = 52/133 (38%), Gaps = 14/133 (10%)
Query: 280 PGSNARARDRVQALQAYYQPQQPPNSTTMRTPIASGTRRPSIHGGSAPLAPV------AT 333
P SN + Q+ Y Q T R P+AS PS+H G AP P+ AT
Sbjct: 44 PSSNTSKDFDPVSGQSNYGGSQGSGQTLNRPPVASNPVTPSLHSGPAPRMPLPASQNPAT 103
Query: 334 SLDQSGGFF----LIPPSSSGRNF----QEENHLPSRYHAWERDHLPSLSLNHVDRDSGW 385
+ S F L PP + N+ + NH P SL+ NH SG+
Sbjct: 104 TPMPSSSFLPEANLPPPLNWQYNYPSTASQTNHCPRASSQPTVSGNTSLTTNHQYVSSGY 163
Query: 386 RAYHQTFSRPDPA 398
+ +F + P+
Sbjct: 164 PSLQNSFIKSGPS 176
>RH3A_ARATH (O22755) RING-H2 zinc finger protein RHA3a
Length = 185
Score = 39.7 bits (91), Expect = 0.015
Identities = 21/59 (35%), Positives = 29/59 (48%), Gaps = 3/59 (5%)
Query: 19 GGGGKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKI 77
G + S C+ICL AD + L CGH FH++CI + CP+CR+I
Sbjct: 91 GAAAEEGDSTECAICLTDFADGEEIRVLPL-CGHSFHVECIDKW--LVSRSSCPSCRRI 146
>RNF4_MOUSE (Q9QZS2) RING finger protein 4
Length = 194
Score = 39.3 bits (90), Expect = 0.019
Identities = 22/59 (37%), Positives = 30/59 (50%), Gaps = 6/59 (10%)
Query: 21 GGKGFGSVSCSICLE---VVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
G + G+VSC IC++ + NG R CGH F C+ + +K A CP CRK
Sbjct: 127 GPRPSGTVSCPICMDGYSEIVQNG-RLIVSTECGHVFCSQCLRDS--LKNANTCPTCRK 182
>RNF4_RAT (O88846) RING finger protein 4 (SNURF)
Length = 194
Score = 38.5 bits (88), Expect = 0.032
Identities = 21/54 (38%), Positives = 28/54 (50%), Gaps = 6/54 (11%)
Query: 26 GSVSCSICLE---VVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
G+VSC IC++ + NG R CGH F C+ + +K A CP CRK
Sbjct: 132 GTVSCPICMDGYSEIVQNG-RLIVSTECGHVFCSQCLRDS--LKNANTCPTCRK 182
>RNF4_HUMAN (P78317) RING finger protein 4
Length = 190
Score = 38.5 bits (88), Expect = 0.032
Identities = 21/54 (38%), Positives = 28/54 (50%), Gaps = 6/54 (11%)
Query: 26 GSVSCSICLE---VVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
G+VSC IC++ + NG R CGH F C+ + +K A CP CRK
Sbjct: 128 GTVSCPICMDGYSEIVQNG-RLIVSTECGHVFCSQCLRDS--LKNANTCPTCRK 178
>RAPS_MOUSE (P12672) 43 kDa receptor-associated protein of the
synapse (RAPsyn) (Acetylcholine receptor-associated 43
kDa protein) (43 kDa postsynaptic protein)
Length = 411
Score = 38.1 bits (87), Expect = 0.042
Identities = 18/47 (38%), Positives = 24/47 (50%), Gaps = 5/47 (10%)
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
C +C E + + R A L C H FHL C+ + G CPNCR+
Sbjct: 362 CGLCGESIGERNSRLQA-LPCSHIFHLRCLQN----NGTRSCPNCRR 403
>RAPS_HUMAN (Q13702) 43 kDa receptor-associated protein of the
synapse (RAPsyn) (Acetylcholine receptor-associated 43
kDa protein) (43 kDa postsynaptic protein)
Length = 411
Score = 38.1 bits (87), Expect = 0.042
Identities = 18/47 (38%), Positives = 24/47 (50%), Gaps = 5/47 (10%)
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
C +C E + + R A L C H FHL C+ + G CPNCR+
Sbjct: 362 CGLCGESIGEKNSRLQA-LPCSHIFHLRCLQN----NGTRSCPNCRR 403
>TM25_MOUSE (Q61510) Tripartite motif-containing protein 25 (Zinc
finger protein 147) (Estrogen responsive finger
protein) (Efp)
Length = 634
Score = 37.7 bits (86), Expect = 0.055
Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 6/51 (11%)
Query: 28 VSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKG-AMQCPNCRKI 77
+SCS+CLE+ + CGH F C+ + ++G +CP CRK+
Sbjct: 11 LSCSVCLELFKEP-----VTTPCGHNFCTSCLDETWVVQGPPYRCPQCRKV 56
>NF7B_XENLA (Q92021) Nuclear factor 7, brain (xnf7) (xnf7-B)
Length = 609
Score = 37.4 bits (85), Expect = 0.072
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 5/58 (8%)
Query: 19 GGGGKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
G G ++C +C+E+ D + CGH F CI A+ + + CP CR+
Sbjct: 134 GAAGDFAEELTCPLCVELFKDP-----VMVACGHNFCRSCIDKAWEGQSSFACPECRE 186
>TRM7_HUMAN (Q9C029) Tripartite motif-containing protein 7
(Glycogenin-interacting protein)
Length = 511
Score = 37.0 bits (84), Expect = 0.094
Identities = 23/74 (31%), Positives = 30/74 (40%), Gaps = 18/74 (24%)
Query: 26 GSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGA-------------MQCP 72
G +CSICLE+ R + CGH F CIG + GA + CP
Sbjct: 25 GEATCSICLELF-----REPVSVECGHSFCRACIGRCWERPGAGSVGAATRAPPFPLPCP 79
Query: 73 NCRKIEKGQWLYAN 86
CR+ + L N
Sbjct: 80 QCREPARPSQLRPN 93
>TM47_HUMAN (Q96LD4) Tripartite motif-containing protein 47 (RING
finger protein 100) (Gene overexpressed in astrocytoma
protein)
Length = 638
Score = 37.0 bits (84), Expect = 0.094
Identities = 22/62 (35%), Positives = 30/62 (47%), Gaps = 14/62 (22%)
Query: 24 GFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIK---------GAMQCPNC 74
G G SC ICLE + R L CGH F L C+G+ + + GA +CP C
Sbjct: 3 GSGPFSCPICLEPL-----REPVTLPCGHNFCLACLGALWPHRGASGAGGPGGAARCPLC 57
Query: 75 RK 76
++
Sbjct: 58 QE 59
>RAPS_CHICK (O42393) 43 kDa receptor-associated protein of the
synapse (RAPsyn) (Acetylcholine receptor-associated 43
kDa protein) (43 kDa postsynaptic protein)
Length = 411
Score = 37.0 bits (84), Expect = 0.094
Identities = 17/48 (35%), Positives = 25/48 (51%), Gaps = 5/48 (10%)
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKI 77
C +C E + + ++ A L C H FHL C+ G CPNCR++
Sbjct: 362 CGMCGESIGEKNNQLQA-LPCSHFFHLKCL----QTNGTRGCPNCRRL 404
>TTC3_HUMAN (P53804) Tetratricopeptide repeat protein 3 (TPR repeat
protein 3) (TPR repeat protein D)
Length = 2025
Score = 36.6 bits (83), Expect = 0.12
Identities = 25/75 (33%), Positives = 33/75 (43%), Gaps = 12/75 (16%)
Query: 26 GSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKIEKGQWLYA 85
G+ SC IC EV R L CGH++H C +KG CP C +G+ L
Sbjct: 1953 GASSCEICHEVFKSKNVRV---LKCGHKYHKGCFKQW--LKGQSACPAC----QGRDLLT 2003
Query: 86 N---GGRSYPEFNMD 97
GR +P N +
Sbjct: 2004 EESPSGRGWPSQNQE 2018
>BSN_RAT (O88778) Bassoon protein
Length = 3937
Score = 36.6 bits (83), Expect = 0.12
Identities = 29/110 (26%), Positives = 47/110 (42%), Gaps = 9/110 (8%)
Query: 217 PFSTASSRLVAADQPSVSPGNQRPVRGGSDVPRSGSFMHPFLVG----HSSAARAGSSVA 272
P + R P SP +G P S + P H+S ++ GS A
Sbjct: 3663 PHARDMGRHETRPHPQASPAPAMQKKGQPGYPSSADYSQPSRAPSAYHHASDSKKGSRQA 3722
Query: 273 SS---LIPPYPGSNARARDRVQALQAYYQPQQPPNSTTMRTPIASGTRRP 319
S ++ P P A+A+ ++Q QA PQQ ++ +TP + +R+P
Sbjct: 3723 HSGPTVLQPKP--EAQAQPQMQGRQAVPGPQQSQPPSSRQTPSGTASRQP 3770
Score = 33.5 bits (75), Expect = 1.0
Identities = 46/196 (23%), Positives = 76/196 (38%), Gaps = 19/196 (9%)
Query: 209 HGWEHHSPPFSTASSRLVAADQPSVSPGNQRPVRGGSDVPRSGSFMHPFLVGHSSAARAG 268
+G+ H P T S A P+ PG P G +P +F HP GH +A
Sbjct: 3066 NGFLAHQAPTYTGPSTYPA---PTYPPGTSYPAEPG--LPSQPAF-HP--TGHYAAPTPM 3117
Query: 269 SSVASSLIPPYPGSNARARDRVQALQAYYQPQQPPNSTTMRTPIASGTRRPSIHGGSAPL 328
+ S+ P S+A + Q A + + P N + +P + + PS G S P
Sbjct: 3118 PTTQSAPFPVQADSHAAHQKPRQTSLADLEQKVPTNYEVISSPAVTVSSTPSETGYSGP- 3176
Query: 329 APVATSLDQS-------GGFFLIPPSSSGRNFQEENHLPSRYHAWERDHLPSLSLNHVDR 381
V++S + GG S + ++H S R+++ ++ + +
Sbjct: 3177 -AVSSSYEHGKAPEHPRGGDRSSVSQSPAPTYPSDSHYTSLEQNVPRNYVMIDDISELTK 3235
Query: 382 DSGWRAYHQTFSRPDP 397
DS A RP+P
Sbjct: 3236 DSTPTA--SDSQRPEP 3249
>YKD4_YEAST (P36096) Hypothetical 87.9 kDa protein in PTM-IRX1
intergenic region precursor
Length = 758
Score = 36.2 bits (82), Expect = 0.16
Identities = 21/66 (31%), Positives = 28/66 (41%), Gaps = 12/66 (18%)
Query: 20 GGGKGFGSVSCSICLEVVA----------DNGDRSWAKLHCGHQFHLDCIGSAFNIKGAM 69
GGG +V C+IC+ V S+ C H FH C+ + N K +
Sbjct: 689 GGGTAEHTVDCAICMSDVPIYIEEIPETHKVDQHSYMVTPCNHVFHTSCLENWMNYK--L 746
Query: 70 QCPNCR 75
QCP CR
Sbjct: 747 QCPVCR 752
>WETA_EMENI (P22022) Regulatory protein wetA
Length = 555
Score = 36.2 bits (82), Expect = 0.16
Identities = 48/198 (24%), Positives = 81/198 (40%), Gaps = 27/198 (13%)
Query: 129 LPSPFDHDMLGQHAIFAEHTAVSSGSHHCPYIAYVGPIHPSSSN--SGGTVSEASNFNHW 186
+PSP + + GQ + T S+ + P PS+ + S S+ + W
Sbjct: 256 MPSPSANALAGQQQRYLSQTGTSA----------LTPSPPSARDVFSSPHSSDPQSMPSW 305
Query: 187 NGSSVPNDMPASFTFPAVDLHYHGWEHHSPPFSTASSRLVAADQPSVSPGNQRPVRGGSD 246
+ S+ + PA P + H W SP S + R + Q SP QRPV+ ++
Sbjct: 306 HSESL--NTPAFQYTPELSDHQTWW---SPMPSEVAQRHASYQQMIASPAPQRPVQAAAN 360
Query: 247 VPRSGSFMHPFLVGHSSAAR--AGSSVASSLIPPYPGSNARARDRVQALQAYYQPQQPPN 304
G F+ L+ + SS SS IP ++ V+A + PQ+ +
Sbjct: 361 ---HGDFLQGGLMIQLDPTQFDISSSFPSSTIPTTANNHDNLAYNVEA----HAPQKYVD 413
Query: 305 STTMRT-PIASGTRRPSI 321
+++ T + +R PSI
Sbjct: 414 ASSFNTQAVPHPSRSPSI 431
>RH3B_ARATH (Q9ZT49) RING-H2 zinc finger protein RHA3b
Length = 200
Score = 36.2 bits (82), Expect = 0.16
Identities = 18/56 (32%), Positives = 28/56 (49%), Gaps = 3/56 (5%)
Query: 22 GKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKI 77
G G S C+IC+ ++ + L C H FH+ CI + + CP+CR+I
Sbjct: 105 GDGDSSTECAICITEFSEGEEIRILPL-CSHAFHVACIDKWLTSRSS--CPSCRRI 157
>RAPS_TORCA (P09108) 43 kDa receptor-associated protein of the
synapse (RAPSYN) (Acetylcholine receptor-associated 43
kDa protein) (43 kDa postsynaptic protein)
Length = 411
Score = 36.2 bits (82), Expect = 0.16
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 5/47 (10%)
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
C +C E + D + A L C H FHL C+ G CPNC++
Sbjct: 362 CGLCGESIGDQNSQLQA-LPCSHLFHLKCL----QTNGNRGCPNCKR 403
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.133 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,575,853
Number of Sequences: 164201
Number of extensions: 2718879
Number of successful extensions: 8988
Number of sequences better than 10.0: 143
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 133
Number of HSP's that attempted gapping in prelim test: 8908
Number of HSP's gapped (non-prelim): 200
length of query: 418
length of database: 59,974,054
effective HSP length: 113
effective length of query: 305
effective length of database: 41,419,341
effective search space: 12632899005
effective search space used: 12632899005
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 67 (30.4 bits)
Lotus: description of TM0299.12