BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0290.16
(172 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SYC_SULTO (Q96YC3) Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cyst... 33 0.33
SPCA_HUMAN (P02549) Spectrin alpha chain, erythrocyte (Erythroid... 31 1.6
YNZ9_CAEEL (P45969) Hypothetical protein T09A5.9 in chromosome III 30 2.1
SYC_AERPE (Q9YBK6) Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cyst... 30 2.1
PERL_HUMAN (P22079) Lactoperoxidase precursor (EC 1.11.1.7) (LPO... 30 2.1
PEPX_STRGN (Q93M42) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11) ... 30 2.1
SYGB_FUSNN (Q8RH44) Glycyl-tRNA synthetase beta chain (EC 6.1.1.... 30 2.8
SYC_SULSO (Q97WE6) Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cyst... 30 2.8
RK5_ODOSI (P49547) Chloroplast 50S ribosomal protein L5 30 2.8
TEST_RAT (P15242) Testin 2 precursor (CMB-23) [Contains: Testin ... 29 4.7
CHD3_CAEEL (Q22516) Chromodomain helicase-DNA-binding protein 3 ... 29 4.7
PEPX_STRPY (Q99Y58) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11) ... 29 6.1
PEPX_STRP8 (Q8NZH6) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11) ... 29 6.1
PEPX_STRP3 (Q8K5X7) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11) ... 29 6.1
GRPE_MYCCA (P71499) GrpE protein (HSP-70 cofactor) 29 6.1
CATM_MOUSE (Q9JL96) Cathepsin M precursor (EC 3.4.22.-) 29 6.1
CATL_SARPE (Q26636) Cathepsin L precursor (EC 3.4.22.15) 29 6.1
APG1_YEAST (P53104) Autophagy serine/threonine-protein kinase AP... 29 6.1
SPEE_AERPE (Q9YE02) Probable spermidine synthase (EC 2.5.1.16) (... 28 8.0
CATK_CHICK (Q90686) Cathepsin K precursor (EC 3.4.22.38) (JTAP-1) 28 8.0
>SYC_SULTO (Q96YC3) Cysteinyl-tRNA synthetase (EC 6.1.1.16)
(Cysteine--tRNA ligase) (CysRS)
Length = 471
Score = 33.1 bits (74), Expect = 0.33
Identities = 36/149 (24%), Positives = 61/149 (40%), Gaps = 16/149 (10%)
Query: 5 HRPNNQAQSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHC---PVLLRQTFQDWGPKPFR 61
H N +AQS L+ +W +W + S L + ++ + L + + WGP
Sbjct: 235 HHENERAQSE---ALLGKQWVKYWVHVSYLTIRKEKMSKSLGNIIPLNEALKKWGPS--- 288
Query: 62 VLNCW-LGDHRLPALVENSWKDMKVKGWGAFMVKEKLKGLRSVLKEWNREVFGDLRSRRE 120
VL W L H +L N + K +K+ + +R V+KE + S+ E
Sbjct: 289 VLRYWYLSSHYRSSLDFNEDSLEQAKN-ALTRLKDAISIIRDVIKEGPK-----YYSKDE 342
Query: 121 VVVTQINNLDLKEEEVGLDQAEVDLRKAL 149
+ Q N ++L + + D KAL
Sbjct: 343 DIQVQRNIVELIKSFHAAMSEDFDTAKAL 371
>SPCA_HUMAN (P02549) Spectrin alpha chain, erythrocyte (Erythroid
alpha-spectrin)
Length = 2418
Score = 30.8 bits (68), Expect = 1.6
Identities = 19/82 (23%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Query: 77 ENSWKDMKVKGWGAFMVKEKLKGLRSVLKEWNREVFGDLRSRREVVVTQINNLDLKEEEV 136
E SW + K+ + L+G++++LK+ R + G+L + + ++ + +++
Sbjct: 1724 EESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKR-LEGELVAHEPAIQNVLDMAEKLKDKA 1782
Query: 137 GLDQAEVDLRKALFNDFWAILR 158
+ Q E+ LR A F + W L+
Sbjct: 1783 AVGQEEIQLRLAQFVEHWEKLK 1804
>YNZ9_CAEEL (P45969) Hypothetical protein T09A5.9 in chromosome III
Length = 326
Score = 30.4 bits (67), Expect = 2.1
Identities = 22/66 (33%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Query: 68 GDHRLPALVENSWKDMKVKGWGAFMVKEKLKGLRSVLKEWNREVFGDLRSRREVV---VT 124
G RL +L + D KV+ + KLKGL++V E N F D R V +T
Sbjct: 252 GVERLESLNDFWANDNKVESFSEIEQLSKLKGLQTVYLERNPFYFNDTNQYRRKVMMTLT 311
Query: 125 QINNLD 130
Q+ +D
Sbjct: 312 QVTQID 317
>SYC_AERPE (Q9YBK6) Cysteinyl-tRNA synthetase (EC 6.1.1.16)
(Cysteine--tRNA ligase) (CysRS)
Length = 475
Score = 30.4 bits (67), Expect = 2.1
Identities = 19/68 (27%), Positives = 32/68 (46%), Gaps = 13/68 (19%)
Query: 5 HRPNNQAQSRLDRVLVSNEWGTFWPNCSQLVL-----NRDVSDHCPVLLRQTFQDWGPKP 59
H N +AQS + W +W + S L + ++ + + P LR+ ++WGP P
Sbjct: 235 HHENERAQSES---YFGHRWVRYWLHASYLTIKGEKMSKSLGNIIP--LREALREWGPGP 289
Query: 60 FRVLNCWL 67
R+ WL
Sbjct: 290 LRL---WL 294
>PERL_HUMAN (P22079) Lactoperoxidase precursor (EC 1.11.1.7) (LPO)
(Salivary peroxidase) (SPO)
Length = 712
Score = 30.4 bits (67), Expect = 2.1
Identities = 23/84 (27%), Positives = 39/84 (46%), Gaps = 11/84 (13%)
Query: 48 LRQTFQDWGPKPFRVLNCWLGDHRLPALVENSWKDMKVKGWGAFMVKEKLKGLRSVLKEW 107
L + +Q WGP+P + L L N+W+ VK G + L +S L +
Sbjct: 478 LDENYQPWGPEP---------ELPLHTLFFNTWR--MVKDGGIDPLVRGLLAKKSKLMKQ 526
Query: 108 NREVFGDLRSRREVVVTQINNLDL 131
N+ + G+LR++ +I+ DL
Sbjct: 527 NKMMTGELRNKLFQPTHRIHGFDL 550
>PEPX_STRGN (Q93M42) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11)
(X-Pro dipeptidyl-peptidase) (X-prolyl-dipeptidyl
aminopeptidase) (X-PDAP) (Sg-xPDPP)
Length = 759
Score = 30.4 bits (67), Expect = 2.1
Identities = 23/96 (23%), Positives = 40/96 (40%), Gaps = 10/96 (10%)
Query: 12 QSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCPVLLRQTFQDWGPKPFRVLNCWLGDHR 71
Q R D + ++ FW + + L+ V V+ QDW KP V N + H
Sbjct: 427 QQRKDLERETGDYNQFWHDRNYLIHADKVK--AEVVFTHGSQDWNVKPLHVYNMF---HA 481
Query: 72 LPALVE-----NSWKDMKVKGWGAFMVKEKLKGLRS 102
LPA ++ ++ + + W + +E + L S
Sbjct: 482 LPAHIKKHLFFHNGAHVYINNWQSIDFRESMNALLS 517
>SYGB_FUSNN (Q8RH44) Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)
(Glycine--tRNA ligase beta chain) (GlyRS)
Length = 686
Score = 30.0 bits (66), Expect = 2.8
Identities = 28/109 (25%), Positives = 49/109 (44%), Gaps = 10/109 (9%)
Query: 46 VLLRQTFQD---WGPKPFRVLNC--WL----GDHRLPALVENSWKDMKVKGWGAFMVKEK 96
++L +TFQ W K R W G + +E K KG F + +
Sbjct: 134 LVLEETFQKSMRWSTKTIRFARPIEWFLALYGGKVIDFEIEGIKSSNKSKGHRFFGKEFE 193
Query: 97 LKGLRSVLKEWNRE-VFGDLRSRREVVVTQINNLDLKEEEVGLDQAEVD 144
+ + + LK+ V D+ RR+++ INN L++E+ +D+A +D
Sbjct: 194 VSSVENYLKKIRENNVIIDISERRKMIEEMINNSLLEDEKADVDKALLD 242
>SYC_SULSO (Q97WE6) Cysteinyl-tRNA synthetase (EC 6.1.1.16)
(Cysteine--tRNA ligase) (CysRS)
Length = 470
Score = 30.0 bits (66), Expect = 2.8
Identities = 26/131 (19%), Positives = 57/131 (42%), Gaps = 15/131 (11%)
Query: 5 HRPNNQAQSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHC---PVLLRQTFQDWGPKPFR 61
H N +AQ+ L+ +W T+W + + + + ++ + L + + WGP
Sbjct: 234 HHENERAQTE---ALIGEKWVTYWVHSAFVTIRKEKMSKSLGNIIPLNEAIKKWGPS--- 287
Query: 62 VLNCWLGDHRLPALVENSWKDMKVKGWGAFMVKEKLKGLRSVLKEWNREVF--GDLRSRR 119
VL W + ++ S + ++ +K+ + +R V+ E + D++ R
Sbjct: 288 VLRYWYLTSHYRSPIDFSEEALEQAKSALQRIKDSMAIIRDVISEGPKFYVKDDDIKVYR 347
Query: 120 EVVVTQINNLD 130
E+ +NNL+
Sbjct: 348 EI----LNNLN 354
>RK5_ODOSI (P49547) Chloroplast 50S ribosomal protein L5
Length = 238
Score = 30.0 bits (66), Expect = 2.8
Identities = 19/59 (32%), Positives = 32/59 (54%), Gaps = 6/59 (10%)
Query: 98 KGLRSVLKEWNREVFGDLRSRREVVVTQINNLDLKEEEVGLDQAEVDLRKALFNDFWAI 156
KG+R+V+K+ N E+FG+ + ++ QIN +GL ++ K N+F AI
Sbjct: 26 KGIRAVIKKNNPELFGNPHTIPKLQKIQIN------RGLGLAAQNTNILKKSINEFTAI 78
>TEST_RAT (P15242) Testin 2 precursor (CMB-23) [Contains: Testin 1
(CMB-22)]
Length = 333
Score = 29.3 bits (64), Expect = 4.7
Identities = 12/34 (35%), Positives = 17/34 (49%)
Query: 19 LVSNEWGTFWPNCSQLVLNRDVSDHCPVLLRQTF 52
LV N WG W + L +D S+HC + T+
Sbjct: 297 LVKNSWGEEWGMKGYMKLAKDWSNHCGIATYSTY 330
>CHD3_CAEEL (Q22516) Chromodomain helicase-DNA-binding protein 3
homolog (CHD-3)
Length = 1787
Score = 29.3 bits (64), Expect = 4.7
Identities = 16/44 (36%), Positives = 22/44 (49%), Gaps = 5/44 (11%)
Query: 29 PNCSQLVLNRDVSDHCPVLLRQTFQDWGPKPFRVLNCWLGDHRL 72
P + L+R SDH P LR+ F +G KP W+ HR+
Sbjct: 468 PIFEESTLSRHHSDHDPYKLRERFYQYGVKPE-----WMQIHRI 506
>PEPX_STRPY (Q99Y58) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11)
(X-Pro dipeptidyl-peptidase) (X-prolyl-dipeptidyl
aminopeptidase) (X-PDAP)
Length = 760
Score = 28.9 bits (63), Expect = 6.1
Identities = 37/146 (25%), Positives = 60/146 (40%), Gaps = 19/146 (13%)
Query: 9 NQAQSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCPVLLRQTFQDWGPKPFRV------ 62
NQ LDR S ++ FW + + L + C V+ QDW KP +V
Sbjct: 428 NQQSQALDRQ--SGDYNQFWHDRNYLKNAHQIK--CDVVYTHGLQDWNVKPRQVYEIFNA 483
Query: 63 ----LNCWLGDHRLPALVENSWKDMKVKGWGAFMVKEKLKGLRS--VLKE--WNREVFGD 114
+N L H+ + ++W+ + + ++ +KL GL + L E W
Sbjct: 484 LPSTINKHLFLHQGEHVYMHNWQSIDFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQ 543
Query: 115 LRSRREVVVTQ-INNLDLKEEEVGLD 139
R+V T I LDL +E + +D
Sbjct: 544 NWQERKVFGTSTIKELDLGQELLLID 569
>PEPX_STRP8 (Q8NZH6) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11)
(X-Pro dipeptidyl-peptidase) (X-prolyl-dipeptidyl
aminopeptidase) (X-PDAP)
Length = 760
Score = 28.9 bits (63), Expect = 6.1
Identities = 37/146 (25%), Positives = 60/146 (40%), Gaps = 19/146 (13%)
Query: 9 NQAQSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCPVLLRQTFQDWGPKPFRV------ 62
NQ LDR S ++ FW + + L + C V+ QDW KP +V
Sbjct: 428 NQQSQALDRQ--SGDYNQFWHDRNYLKNAHQIK--CDVVYTHGLQDWNVKPRQVYEIFNA 483
Query: 63 ----LNCWLGDHRLPALVENSWKDMKVKGWGAFMVKEKLKGLRS--VLKE--WNREVFGD 114
+N L H+ + ++W+ + + ++ +KL GL + L E W
Sbjct: 484 LPSTINKHLFLHQGEHVYMHNWQSIDFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQ 543
Query: 115 LRSRREVVVTQ-INNLDLKEEEVGLD 139
R+V T I LDL +E + +D
Sbjct: 544 NWQERKVFGTSTIKELDLGQELLLID 569
>PEPX_STRP3 (Q8K5X7) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11)
(X-Pro dipeptidyl-peptidase) (X-prolyl-dipeptidyl
aminopeptidase) (X-PDAP)
Length = 760
Score = 28.9 bits (63), Expect = 6.1
Identities = 37/146 (25%), Positives = 60/146 (40%), Gaps = 19/146 (13%)
Query: 9 NQAQSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCPVLLRQTFQDWGPKPFRV------ 62
NQ LDR S ++ FW + + L + C V+ QDW KP +V
Sbjct: 428 NQQSQALDRQ--SGDYNQFWHDRNYLKNAHQIK--CDVVYTHGLQDWNVKPRQVYEIVNA 483
Query: 63 ----LNCWLGDHRLPALVENSWKDMKVKGWGAFMVKEKLKGLRS--VLKE--WNREVFGD 114
+N L H+ + ++W+ + + ++ +KL GL + L E W
Sbjct: 484 LPSTINKHLFLHQGEHVYMHNWQSIDFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQ 543
Query: 115 LRSRREVVVTQ-INNLDLKEEEVGLD 139
R+V T I LDL +E + +D
Sbjct: 544 NWQERKVFGTSTIKELDLGQELLLID 569
>GRPE_MYCCA (P71499) GrpE protein (HSP-70 cofactor)
Length = 206
Score = 28.9 bits (63), Expect = 6.1
Identities = 20/75 (26%), Positives = 38/75 (50%), Gaps = 12/75 (16%)
Query: 73 PALVENSWKDMKVKGWGAFMVKEKLKGLRSVLKEWNREVFGDLRSRREVVVTQINNLDLK 132
P + +N + +K K A + + LK L LK+ D+ S R++ + +I+NL K
Sbjct: 29 PHVKKNQYLKLKTKLDTALLEVQNLKDLNETLKK-------DIESERQLNLAEISNLTKK 81
Query: 133 EEEVGLDQAEVDLRK 147
+Q E++++K
Sbjct: 82 -----YNQKEIEIQK 91
>CATM_MOUSE (Q9JL96) Cathepsin M precursor (EC 3.4.22.-)
Length = 333
Score = 28.9 bits (63), Expect = 6.1
Identities = 11/28 (39%), Positives = 16/28 (56%)
Query: 19 LVSNEWGTFWPNCSQLVLNRDVSDHCPV 46
LV N GT W N + ++RD +HC +
Sbjct: 297 LVKNSMGTQWGNKGYMKISRDKGNHCGI 324
>CATL_SARPE (Q26636) Cathepsin L precursor (EC 3.4.22.15)
Length = 339
Score = 28.9 bits (63), Expect = 6.1
Identities = 11/41 (26%), Positives = 21/41 (50%)
Query: 12 QSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCPVLLRQTF 52
+S +D LV N WGT W + + R+ ++ C + ++
Sbjct: 296 ESGMDYWLVKNSWGTTWGEQGYIKMARNQNNQCGIATASSY 336
>APG1_YEAST (P53104) Autophagy serine/threonine-protein kinase APG1
(EC 2.7.1.37)
Length = 897
Score = 28.9 bits (63), Expect = 6.1
Identities = 24/82 (29%), Positives = 38/82 (46%), Gaps = 11/82 (13%)
Query: 81 KDMKVKGWGAFMVKEKLKGLR-SVLKEWNREVFGDLRSRREVVVTQINNLDLKE------ 133
K M+V + +EK LR +VL +W RE F + + + + +IN+L K
Sbjct: 714 KSMQVTSNWWYESQEKSCSLRVNVLVQWLREKFNECLEKADFLRLKINDLRFKHASEVAE 773
Query: 134 ----EEVGLDQAEVDLRKALFN 151
EE G + V L K L++
Sbjct: 774 NQTLEEKGSSEEPVYLEKLLYD 795
>SPEE_AERPE (Q9YE02) Probable spermidine synthase (EC 2.5.1.16)
(Putrescine aminopropyltransferase) (SPDSY)
Length = 306
Score = 28.5 bits (62), Expect = 8.0
Identities = 17/45 (37%), Positives = 21/45 (45%), Gaps = 2/45 (4%)
Query: 101 RSVLKEWNREVFGDLRSRREVVVTQINNLDLKEEEVGLDQAEVDL 145
R L+EW+R F D R EVV+ N + E G D DL
Sbjct: 119 REHLREWHRGAFDD--PRAEVVIDDAWNYVASKAETGFDVVIADL 161
>CATK_CHICK (Q90686) Cathepsin K precursor (EC 3.4.22.38) (JTAP-1)
Length = 334
Score = 28.5 bits (62), Expect = 8.0
Identities = 11/36 (30%), Positives = 18/36 (49%)
Query: 11 AQSRLDRVLVSNEWGTFWPNCSQLVLNRDVSDHCPV 46
AQ ++ N WGT W N ++L R++ C +
Sbjct: 290 AQKGTKHWIIKNSWGTEWGNKGYVLLARNMKQTCGI 325
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.138 0.460
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,240,407
Number of Sequences: 164201
Number of extensions: 800025
Number of successful extensions: 1830
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 1826
Number of HSP's gapped (non-prelim): 20
length of query: 172
length of database: 59,974,054
effective HSP length: 102
effective length of query: 70
effective length of database: 43,225,552
effective search space: 3025788640
effective search space used: 3025788640
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0290.16