BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0290.12
(143 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PSBS_TOBAC (Q9SMB4) Photosystem II 22 kDa protein, chloroplast p... 42 6e-04
ELI5_HORVU (P14895) High molecular mass early light-inducible pr... 39 0.003
YC17_PORPU (P51355) Hypothetical 5.5 kDa protein ycf17 (ORF48) 38 0.006
PSBS_SOLSG (Q9FPP4) Photosystem II 22 kDa protein, chloroplast p... 38 0.006
PSBS_LYCES (P54773) Photosystem II 22 kDa protein, chloroplast p... 38 0.006
PSBS_ARATH (Q9XF91) Photosystem II 22 kDa protein, chloroplast p... 38 0.006
ELI_PEA (P11432) Early light-induced protein, chloroplast precur... 38 0.006
PSBS_SPIOL (Q02060) Photosystem II 22 kDa protein, chloroplast p... 37 0.011
YC17_CYAPA (P48367) Hypothetical 5.5 kDa protein ycf17 37 0.014
HEMZ_ANASP (Q8YQR8) Ferrochelatase (EC 4.99.1.1) (Protoheme ferr... 36 0.024
HMZ2_ARATH (O04921) Ferrochelatase II, chloroplast precursor (EC... 35 0.071
ELI6_HORVU (P14896) Low molecular mass early light-inducible pro... 34 0.093
ELI9_HORVU (P14897) Low molecular mass early light-inducible pro... 34 0.12
HPPA_PYRAE (Q8ZWI8) Pyrophosphate-energized proton pump (EC 3.6.... 33 0.16
PGCA_CHICK (P07898) Aggrecan core protein precursor (Cartilage-s... 33 0.27
HEMZ_ORYSA (O22101) Ferrochelatase II, chloroplast precursor (EC... 33 0.27
HEMZ_SYNPX (Q7U5G0) Ferrochelatase (EC 4.99.1.1) (Protoheme ferr... 32 0.35
DS22_CRAPL (Q01931) Desiccation stress protein DSP-22, chloropla... 32 0.35
CBR_DUNBA (P27516) Carotene biosynthesis-related protein CBR, ch... 32 0.35
HEMZ_SYNY3 (P54225) Ferrochelatase (EC 4.99.1.1) (Protoheme ferr... 32 0.46
>PSBS_TOBAC (Q9SMB4) Photosystem II 22 kDa protein, chloroplast
precursor (CP22)
Length = 274
Score = 41.6 bits (96), Expect = 6e-04
Identities = 27/95 (28%), Positives = 50/95 (52%), Gaps = 14/95 (14%)
Query: 35 PNLVPSRASFATGTPLLIRRSHGRKLACRAMPVSIRSEQSTKDG-----NGL------DV 83
P+ S +S T T + + +S + A + +P + ++ +DG G+ ++
Sbjct: 43 PSASASASSHFTSTTVALFKSKAKAPAKKVVP---KPKEKVEDGIFGTSGGIGFTKQNEL 99
Query: 84 WLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++GR+AMIGFAA + E TGKG+L + + +P
Sbjct: 100 FVGRVAMIGFAASLLGEAITGKGILAQLNLETGIP 134
Score = 32.7 bits (73), Expect = 0.27
Identities = 19/59 (32%), Positives = 30/59 (50%), Gaps = 3/59 (5%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIFQSA 140
++++GRLA +G A I E+ TGKG L + +P + L + VF F +A
Sbjct: 204 ELFVGRLAQLGIAFSIIGEIITGKGALAQLNFETGVPINEIEPLLLFNI---VFFFVAA 259
>ELI5_HORVU (P14895) High molecular mass early light-inducible
protein HV58, chloroplast precursor (ELIP)
Length = 231
Score = 39.3 bits (90), Expect = 0.003
Identities = 22/56 (39%), Positives = 31/56 (55%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIFQSAS 141
GRLAM+GF A ++VE A G GLL+ G+ S LA + V + + + Q S
Sbjct: 134 GRLAMVGFVAALSVEAARGGGLLDQVGMWSSGLAWFLATAGVFSVASLLPLLQGQS 189
>YC17_PORPU (P51355) Hypothetical 5.5 kDa protein ycf17 (ORF48)
Length = 48
Score = 38.1 bits (87), Expect = 0.006
Identities = 17/27 (62%), Positives = 20/27 (73%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLL 108
+ W GR AMIGF A I +E+ TGKGLL
Sbjct: 15 ETWNGRFAMIGFMAVIFIELVTGKGLL 41
>PSBS_SOLSG (Q9FPP4) Photosystem II 22 kDa protein, chloroplast
precursor (CP22)
Length = 276
Score = 38.1 bits (87), Expect = 0.006
Identities = 16/37 (43%), Positives = 26/37 (70%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++++GR+AMIGFAA + E TGKG+L + + +P
Sbjct: 100 ELFVGRVAMIGFAASLLGEAITGKGILAQLNLETGIP 136
Score = 31.6 bits (70), Expect = 0.60
Identities = 14/37 (37%), Positives = 22/37 (58%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++++GRLA +G A I E+ TGKG L + +P
Sbjct: 206 ELFVGRLAQLGIAFSIIGEIITGKGALAQLNFETGVP 242
>PSBS_LYCES (P54773) Photosystem II 22 kDa protein, chloroplast
precursor (CP22)
Length = 276
Score = 38.1 bits (87), Expect = 0.006
Identities = 16/37 (43%), Positives = 26/37 (70%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++++GR+AMIGFAA + E TGKG+L + + +P
Sbjct: 100 ELFVGRVAMIGFAASLLGEAITGKGILAQLNLETGIP 136
Score = 31.6 bits (70), Expect = 0.60
Identities = 14/37 (37%), Positives = 22/37 (58%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++++GRLA +G A I E+ TGKG L + +P
Sbjct: 206 ELFVGRLAQLGIAFSIIGEIITGKGALAQLNFETGVP 242
>PSBS_ARATH (Q9XF91) Photosystem II 22 kDa protein, chloroplast
precursor (CP22)
Length = 265
Score = 38.1 bits (87), Expect = 0.006
Identities = 16/37 (43%), Positives = 26/37 (70%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++++GR+AMIGFAA + E TGKG+L + + +P
Sbjct: 90 ELFVGRVAMIGFAASLLGEALTGKGILAQLNLETGIP 126
Score = 34.3 bits (77), Expect = 0.093
Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIF 137
++++GRLA +G A + E+ TGKG L + + +P + L+ V A F F
Sbjct: 194 ELFVGRLAQLGIAFSLIGEIITGKGALAQLNIETGIPIQDIEPLVLLNV--AFFFF 247
>ELI_PEA (P11432) Early light-induced protein, chloroplast precursor
(ELIP)
Length = 196
Score = 38.1 bits (87), Expect = 0.006
Identities = 28/62 (45%), Positives = 38/62 (61%), Gaps = 5/62 (8%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENF---GVASPLPT-VALAVTALVGVLTAVFIFQSAS 141
GRLAMIGF A + VE+A G+GL E GVA L T V L++ +L+ V + +S S
Sbjct: 103 GRLAMIGFVAAMGVEIAKGQGLSEQLSGGGVAWFLGTSVLLSLASLIPFFQGVSV-ESKS 161
Query: 142 KN 143
K+
Sbjct: 162 KS 163
>PSBS_SPIOL (Q02060) Photosystem II 22 kDa protein, chloroplast
precursor (CP22)
Length = 274
Score = 37.4 bits (85), Expect = 0.011
Identities = 16/37 (43%), Positives = 26/37 (70%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLP 118
++++GR+AMIGFAA + E TGKG+L + + +P
Sbjct: 99 ELFVGRVAMIGFAASLLGEGITGKGILSQLNLETGIP 135
Score = 37.0 bits (84), Expect = 0.014
Identities = 20/59 (33%), Positives = 33/59 (55%), Gaps = 3/59 (5%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIFQSA 140
++++GRLA +GFA + E+ TGKG L + + +P + L+ V VF F +A
Sbjct: 203 ELFVGRLAQLGFAFSLIGEIITGKGALAQLNIETGVPINEIEPLVLLNV---VFFFIAA 258
>YC17_CYAPA (P48367) Hypothetical 5.5 kDa protein ycf17
Length = 49
Score = 37.0 bits (84), Expect = 0.014
Identities = 16/35 (45%), Positives = 22/35 (62%)
Query: 79 NGLDVWLGRLAMIGFAAGITVEVATGKGLLENFGV 113
+G + W GRLAM+GF A + E TG+G L G+
Sbjct: 14 SGAENWNGRLAMLGFIAALLTESLTGQGTLHFLGI 48
>HEMZ_ANASP (Q8YQR8) Ferrochelatase (EC 4.99.1.1) (Protoheme
ferro-lyase) (Heme synthetase)
Length = 388
Score = 36.2 bits (82), Expect = 0.024
Identities = 16/32 (50%), Positives = 24/32 (75%), Gaps = 1/32 (3%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGV 113
+VW GR+AM+GF A + +E+ TG+GLL G+
Sbjct: 356 EVWNGRIAMLGFIA-LIIELVTGQGLLHMIGL 386
>HMZ2_ARATH (O04921) Ferrochelatase II, chloroplast precursor (EC
4.99.1.1) (Protoheme ferro-lyase) (Heme synthetase)
Length = 512
Score = 34.7 bits (78), Expect = 0.071
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGVASPL 117
+ W GR AM+ A + +EV TGKG L +G+ L
Sbjct: 477 ETWNGRAAMLAVLALLVLEVTTGKGFLHQWGILPSL 512
>ELI6_HORVU (P14896) Low molecular mass early light-inducible
protein HV60, chloroplast precursor (ELIP)
Length = 167
Score = 34.3 bits (77), Expect = 0.093
Identities = 20/56 (35%), Positives = 28/56 (49%), Gaps = 1/56 (1%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIFQSAS 141
GRLAM+GF + VE G GLL G + A V A++ + + V + Q S
Sbjct: 71 GRLAMVGFVTALAVEAGRGDGLLSQLGSGTGQAWFAYTV-AMLSMASLVPLLQGES 125
>ELI9_HORVU (P14897) Low molecular mass early light-inducible
protein HV90, chloroplast precursor (ELIP)
Length = 172
Score = 33.9 bits (76), Expect = 0.12
Identities = 17/42 (40%), Positives = 20/42 (47%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTAL 127
GRLAM+GF + VE G GLL G + A V L
Sbjct: 76 GRLAMVGFVTALAVEAGRGDGLLSQLGSGTGQAWFAYTVAVL 117
>HPPA_PYRAE (Q8ZWI8) Pyrophosphate-energized proton pump (EC
3.6.1.1) (Pyrophosphate-energized inorganic
pyrophosphatase) (H+-PPase) (Membrane-bound
proton-translocating pyrophosphatase)
Length = 721
Score = 33.5 bits (75), Expect = 0.16
Identities = 18/47 (38%), Positives = 27/47 (57%)
Query: 89 AMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVF 135
A+I FAA V G LL+ GV PL +++LA+ A +G+ +F
Sbjct: 263 AIILFAALALVATFAGVNLLKTTGVKHPLSSISLAIYATIGLSVVLF 309
>PGCA_CHICK (P07898) Aggrecan core protein precursor
(Cartilage-specific proteoglycan core protein) (CSPCP)
Length = 2109
Score = 32.7 bits (73), Expect = 0.27
Identities = 23/65 (35%), Positives = 29/65 (44%), Gaps = 8/65 (12%)
Query: 86 GRLAMIGFAAGIT--------VEVATGKGLLENFGVASPLPTVALAVTALVGVLTAVFIF 137
G L M GF G VEV +G E G+ S PTV+L T LV V+T +
Sbjct: 1166 GVLEMSGFPGGAVESSGEASGVEVTSGLASGEESGLTSGFPTVSLVDTTLVEVVTQTSVA 1225
Query: 138 QSASK 142
Q +
Sbjct: 1226 QEVGE 1230
Score = 27.7 bits (60), Expect = 8.7
Identities = 17/43 (39%), Positives = 26/43 (59%), Gaps = 1/43 (2%)
Query: 92 GFAAGIT-VEVATGKGLLENFGVASPLPTVALAVTALVGVLTA 133
GF+ I+ E+ +G E G+AS PT++L + LV V+TA
Sbjct: 921 GFSGEISGTELISGLPSGEESGLASGFPTISLVDSTLVEVVTA 963
>HEMZ_ORYSA (O22101) Ferrochelatase II, chloroplast precursor (EC
4.99.1.1) (Protoheme ferro-lyase) (Heme synthetase)
(Fragment)
Length = 494
Score = 32.7 bits (73), Expect = 0.27
Identities = 13/32 (40%), Positives = 20/32 (61%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGV 113
+ W GR AM+ A + +EV TG+G L +G+
Sbjct: 458 ETWNGRAAMLAVLALLVLEVTTGEGFLHQWGI 489
>HEMZ_SYNPX (Q7U5G0) Ferrochelatase (EC 4.99.1.1) (Protoheme
ferro-lyase) (Heme synthetase)
Length = 391
Score = 32.3 bits (72), Expect = 0.35
Identities = 16/35 (45%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Query: 79 NGLDVWLGRLAMIGFAAGITVEVATGKGLLENFGV 113
N +VW GRLAM+GF+A +E+ +G G L G+
Sbjct: 357 NSSEVWNGRLAMLGFSA-FLLELISGHGPLHALGL 390
>DS22_CRAPL (Q01931) Desiccation stress protein DSP-22, chloroplast
precursor
Length = 199
Score = 32.3 bits (72), Expect = 0.35
Identities = 21/44 (47%), Positives = 26/44 (58%), Gaps = 3/44 (6%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLE---NFGVASPLPTVALAVTA 126
GR AMIGF A + VE+ATG+ + N GV L T A+ V A
Sbjct: 104 GRSAMIGFVAAVGVELATGRDVFSQVFNGGVMWFLLTSAVLVLA 147
>CBR_DUNBA (P27516) Carotene biosynthesis-related protein CBR,
chloroplast precursor
Length = 172
Score = 32.3 bits (72), Expect = 0.35
Identities = 15/48 (31%), Positives = 26/48 (53%)
Query: 86 GRLAMIGFAAGITVEVATGKGLLENFGVASPLPTVALAVTALVGVLTA 133
GRLAM+GF A + E++TG+ +L G L + + + ++ A
Sbjct: 82 GRLAMLGFVAALGAELSTGESVLTQLGDQPTLIALTFVLFSAASLIPA 129
>HEMZ_SYNY3 (P54225) Ferrochelatase (EC 4.99.1.1) (Protoheme
ferro-lyase) (Heme synthetase)
Length = 387
Score = 32.0 bits (71), Expect = 0.46
Identities = 16/32 (50%), Positives = 23/32 (71%), Gaps = 1/32 (3%)
Query: 82 DVWLGRLAMIGFAAGITVEVATGKGLLENFGV 113
+VW GRLAM+GF A + VE+ +G+G L G+
Sbjct: 356 EVWNGRLAMLGFIA-LLVELISGQGPLHFVGL 386
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.133 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,142,628
Number of Sequences: 164201
Number of extensions: 533257
Number of successful extensions: 1397
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 1362
Number of HSP's gapped (non-prelim): 51
length of query: 143
length of database: 59,974,054
effective HSP length: 99
effective length of query: 44
effective length of database: 43,718,155
effective search space: 1923598820
effective search space used: 1923598820
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0290.12