BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0265.9
(456 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
GSAB_PICPA (Q9HFR4) Pexophagy regulatory protein Gsa11 33 2.0
YC14_PYRHO (O58969) Hypothetical ABC transporter extracellular b... 32 2.6
POLG_HCVJA (P26662) Genome polyprotein [Contains: Capsid protein... 32 4.4
2ABA_CANTR (P53031) Protein phosphatase PP2A regulatory subunit ... 32 4.4
CRTI_ORYSA (Q9ZTN9) Phytoene dehydrogenase, chloroplast precurso... 31 5.8
CRTI_NARPS (Q40406) Phytoene dehydrogenase, chloroplast precurso... 31 5.8
CRTI_LYCES (P28554) Phytoene dehydrogenase, chloroplast precurso... 31 5.8
VPS8_YEAST (P39702) Vacuolar protein sorting-associated protein ... 31 7.5
RR2_WHEAT (P17933) Chloroplast 30S ribosomal protein S2 31 7.5
RR2_ORYSA (P12145) Chloroplast 30S ribosomal protein S2 31 7.5
RR2_MAIZE (P16037) Chloroplast 30S ribosomal protein S2 31 7.5
CRTI_SOYBN (P28553) Phytoene dehydrogenase, chloroplast precurso... 31 7.5
ZFY1_XENLA (Q01611) Zinc finger Y-chromosomal protein 1 30 9.8
YAE6_SCHPO (Q09847) Hypothetical protein C23D3.06c in chromosome I 30 9.8
RR2_OENHO (Q9MTM2) Chloroplast 30S ribosomal protein S2 30 9.8
POLG_HCVTW (P29846) Genome polyprotein [Contains: Capsid protein... 30 9.8
POL2_BAMMN (P89684) Genome polyprotein 2 [Contains: Helper compo... 30 9.8
CRTI_CAPAN (P80093) Phytoene dehydrogenase, chloroplast precurso... 30 9.8
CRTI_ARATH (Q07356) Phytoene dehydrogenase, chloroplast precurso... 30 9.8
>GSAB_PICPA (Q9HFR4) Pexophagy regulatory protein Gsa11
Length = 1862
Score = 32.7 bits (73), Expect = 2.0
Identities = 27/96 (28%), Positives = 45/96 (46%), Gaps = 10/96 (10%)
Query: 91 KEIKNFRKSSTSSTKMLGYMHGNADSFGGNLSTHLRFSYFDHQNDSTEVPCGFFKKFPIS 150
K++K F KS + Y+ NA + + S F DS E+ FF++ +
Sbjct: 700 KKVKTFDKSGIQCS----YLSPNASEIE-LIELEMTMSDFTSLIDSLEL---FFEQLKVV 751
Query: 151 DSDRIAMEKCKNVVVVSAIFNDHDKIRQPKGLGSKT 186
+ ++ + K K V S FN + +R P+ LGS+T
Sbjct: 752 NHPKMDIAKEKTVFRTS--FNSRNPVRNPRALGSQT 785
>YC14_PYRHO (O58969) Hypothetical ABC transporter extracellular
binding protein PH1214 precursor
Length = 441
Score = 32.3 bits (72), Expect = 2.6
Identities = 18/65 (27%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Query: 46 SSPFDPIQTSLISYPSSYGEHKYAVSTTRSSCSSPVLFSDYRDVLKEIKNFRKSSTSSTK 105
+ P+DPIQ S I SS E K S S +D D++ E+ + + ++T+
Sbjct: 363 ADPYDPIQKSFIKELSS-SETKLIPSIAHGSAVPEAFLADLNDIISELATSKDVNKAATE 421
Query: 106 MLGYM 110
++ M
Sbjct: 422 IINAM 426
>POLG_HCVJA (P26662) Genome polyprotein [Contains: Capsid protein C
(Core protein) (P22); Envelope glycoprotein E1 (GP32)
(GP35); Envelope glycoprotein E2 (GP68) (GP70) (NS1);
Protein P7; Nonstructural protein NS2 (P21) (EC
3.4.22.-); Protease/helicase NS
Length = 3010
Score = 31.6 bits (70), Expect = 4.4
Identities = 30/103 (29%), Positives = 44/103 (42%), Gaps = 28/103 (27%)
Query: 17 LLCFSLFYLFTSLFLALYTISFQSKCLFRSS--PFDPIQTSLISYPSSYGEHKYAVSTTR 74
++C S+ Y +T + + C S P +P+ SL+ + H STT
Sbjct: 2416 VVCCSMSYTWTGALI--------TPCAAEESKLPINPLSNSLLRH------HSMVYSTTS 2461
Query: 75 SSCSS----------PVLFSDYRDVLKEIKNFRKSSTSSTKML 107
S S VL YRDVLKE+K K+ST ++L
Sbjct: 2462 RSASLRQKKVTFDRLQVLDDHYRDVLKEMK--AKASTVKARLL 2502
>2ABA_CANTR (P53031) Protein phosphatase PP2A regulatory subunit B
(PR55) (Cell division control protein 55)
Length = 508
Score = 31.6 bits (70), Expect = 4.4
Identities = 22/67 (32%), Positives = 31/67 (45%), Gaps = 3/67 (4%)
Query: 46 SSPFDPIQTSLISYPSSYGEHKYAVSTTRSSCSSPV-LFSDYRDVLKEIKNFRKSSTSST 104
S+ F P+Q +L Y SS G K + + S C S +F +Y D NF TSS
Sbjct: 222 SAEFHPLQCNLFMYSSSKGTIKLSDMRSNSLCDSHAKIFEEYLD--PSSHNFFTEITSSI 279
Query: 105 KMLGYMH 111
+ + H
Sbjct: 280 SDVKFSH 286
>CRTI_ORYSA (Q9ZTN9) Phytoene dehydrogenase, chloroplast precursor
(EC 1.14.99.-) (Phytoene desaturase)
Length = 566
Score = 31.2 bits (69), Expect = 5.8
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 258 IWIDAKLKLMVDPLLLIHSLVISENADMAISKHPFY 293
IW D KLK D LL S ++S ADM+++ +Y
Sbjct: 396 IWFDRKLKNTYDHLLFSRSSLLSVYADMSVTCKEYY 431
>CRTI_NARPS (Q40406) Phytoene dehydrogenase, chloroplast precursor
(EC 1.14.99.-) (Phytoene desaturase)
Length = 570
Score = 31.2 bits (69), Expect = 5.8
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 258 IWIDAKLKLMVDPLLLIHSLVISENADMAISKHPFY 293
IW D KLK D LL S ++S ADM+++ +Y
Sbjct: 401 IWFDRKLKNTYDHLLFTRSPLLSVYADMSVTCKEYY 436
>CRTI_LYCES (P28554) Phytoene dehydrogenase, chloroplast precursor
(EC 1.14.99.-) (Phytoene desaturase)
Length = 583
Score = 31.2 bits (69), Expect = 5.8
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 258 IWIDAKLKLMVDPLLLIHSLVISENADMAISKHPFY 293
IW D KLK D LL S ++S ADM+++ +Y
Sbjct: 415 IWFDRKLKNTYDHLLFSRSSLLSVYADMSVTCKEYY 450
>VPS8_YEAST (P39702) Vacuolar protein sorting-associated protein
VPS8
Length = 1274
Score = 30.8 bits (68), Expect = 7.5
Identities = 40/156 (25%), Positives = 63/156 (39%), Gaps = 14/156 (8%)
Query: 261 DAKLKLMVDPLLLIHSLVISENADMAISKHPFYVHTMEEAMATARWKKWWDINALKMQME 320
D K+ L +D LIH L+I N IS+ FY+ T + ++ W DI +
Sbjct: 378 DFKILLRLD--FLIHDLMIPPNKYFVISRRSFYLLT-NYSFKIGKFVSWSDITLRHILKG 434
Query: 321 TY------CENGLQPWNP----SKLPYVSDVPDSALILRKHGLGSNLFSCLVFNELEAFN 370
Y E+ LQP+ P KL ++ L+ + L L+ + +N
Sbjct: 435 DYLGALEFIESLLQPYCPLANLLKLDNNTEERTKQLMEPFYNLSLAALRFLIKKDNADYN 494
Query: 371 PRDQLAFAFVRDNMNPKLKLNMF-EVEVFEQVTMEF 405
QL VR KL+ ++VF + +EF
Sbjct: 495 RVYQLLMVVVRVLQQSSKKLDSIPSLDVFLEQGLEF 530
>RR2_WHEAT (P17933) Chloroplast 30S ribosomal protein S2
Length = 236
Score = 30.8 bits (68), Expect = 7.5
Identities = 11/34 (32%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Query: 307 KKWWDINALKM-QMETYCENGLQPWNPSKLPYVS 339
+++W+IN +M + + +G++ WNP PY+S
Sbjct: 3 RRYWNINLKEMIEAGVHFGHGIKKWNPKMAPYIS 36
>RR2_ORYSA (P12145) Chloroplast 30S ribosomal protein S2
Length = 236
Score = 30.8 bits (68), Expect = 7.5
Identities = 11/34 (32%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Query: 307 KKWWDINALKM-QMETYCENGLQPWNPSKLPYVS 339
+++W+IN +M + + +G++ WNP PY+S
Sbjct: 3 RRYWNINLKEMIEAGVHFGHGIKKWNPKMAPYIS 36
>RR2_MAIZE (P16037) Chloroplast 30S ribosomal protein S2
Length = 236
Score = 30.8 bits (68), Expect = 7.5
Identities = 11/34 (32%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Query: 307 KKWWDINALKM-QMETYCENGLQPWNPSKLPYVS 339
+++W+IN +M + + +G++ WNP PY+S
Sbjct: 3 RRYWNINLKEMIEAGVHFGHGIKKWNPKMAPYIS 36
>CRTI_SOYBN (P28553) Phytoene dehydrogenase, chloroplast precursor
(EC 1.14.99.-) (Phytoene desaturase)
Length = 570
Score = 30.8 bits (68), Expect = 7.5
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 258 IWIDAKLKLMVDPLLLIHSLVISENADMAISKHPFY 293
IW D KLK D LL S ++S ADM+++ +Y
Sbjct: 402 IWFDRKLKNTYDHLLFSRSPLLSVYADMSVTCKEYY 437
>ZFY1_XENLA (Q01611) Zinc finger Y-chromosomal protein 1
Length = 794
Score = 30.4 bits (67), Expect = 9.8
Identities = 12/45 (26%), Positives = 24/45 (52%)
Query: 132 HQNDSTEVPCGFFKKFPISDSDRIAMEKCKNVVVVSAIFNDHDKI 176
H+NDS EV C + + D+++I + + + + S + D K+
Sbjct: 191 HENDSEEVNCDDYLMISLDDAEKIDEDGAEEITMGSVVEGDSSKL 235
>YAE6_SCHPO (Q09847) Hypothetical protein C23D3.06c in chromosome I
Length = 1325
Score = 30.4 bits (67), Expect = 9.8
Identities = 22/63 (34%), Positives = 30/63 (46%), Gaps = 3/63 (4%)
Query: 362 VFNELEAFNPRDQLAFAFVRDNMNPKLKLNMFEVEVFEQVTMEFRHNLGLSNGSTAKKLS 421
VF + AF Q +F+F NMN KL F+ + T E + NL + S + K S
Sbjct: 541 VFGQPSAFG---QSSFSFGTSNMNQKLDFGTFKSPLSGAATGEAKTNLEKAVTSASGKAS 597
Query: 422 RTG 424
TG
Sbjct: 598 FTG 600
>RR2_OENHO (Q9MTM2) Chloroplast 30S ribosomal protein S2
Length = 236
Score = 30.4 bits (67), Expect = 9.8
Identities = 11/33 (33%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Query: 307 KKWWDINALKM-QMETYCENGLQPWNPSKLPYV 338
+++W+IN +M + Y +G++ WNP PY+
Sbjct: 3 RRYWNINLEEMMEAGVYFGHGIKKWNPRMAPYI 35
>POLG_HCVTW (P29846) Genome polyprotein [Contains: Capsid protein C
(Core protein) (P22); Envelope glycoprotein E1 (GP32)
(GP35); Envelope glycoprotein E2 (GP68) (GP70) (NS1);
Protein P7; Nonstructural protein NS2 (P21) (EC
3.4.22.-); Protease/helicase NS
Length = 3010
Score = 30.4 bits (67), Expect = 9.8
Identities = 39/154 (25%), Positives = 57/154 (36%), Gaps = 47/154 (30%)
Query: 17 LLCFSLFYLFTSLFLALYTISFQSKCLFRSS--PFDPIQTSLISYPSSYGEHKYAVSTTR 74
++C S+ Y +T + + C S P + + SL+ + H +TT
Sbjct: 2416 VICCSMSYTWTGALI--------TPCAAEESKLPINALSNSLLRH------HNMVYATTS 2461
Query: 75 SSCSS----------PVLFSDYRDVLKEIKNFRKSSTSSTKML----------------- 107
S S VL YRDVLKE+K K+ST K+L
Sbjct: 2462 RSASQRQKKVTIDRLQVLDDHYRDVLKEMK--AKASTVKAKLLSVEEACKLTPPHSARSK 2519
Query: 108 -GYMHGNADSFGGNLSTHLRFSYFDHQNDSTEVP 140
GY + + G H+R + D D TE P
Sbjct: 2520 FGYGAKDVRNLSGKAINHIRSVWKDLLED-TETP 2552
>POL2_BAMMN (P89684) Genome polyprotein 2 [Contains: Helper
component proteinase (EC 3.4.22.45) (HC-pro); 70 kDa
protein]
Length = 891
Score = 30.4 bits (67), Expect = 9.8
Identities = 12/40 (30%), Positives = 24/40 (60%)
Query: 220 GVWRVVKVSKEYLYLNPAMNGVIPKYLVHRLFPNSQFSIW 259
G+W ++K + Y L+ A +GV +Y+++ + Q+S W
Sbjct: 198 GMWNILKAGRVYELLSLAADGVGCEYMLYPVGAAPQYSFW 237
>CRTI_CAPAN (P80093) Phytoene dehydrogenase, chloroplast precursor
(EC 1.14.99.-) (Phytoene desaturase)
Length = 582
Score = 30.4 bits (67), Expect = 9.8
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 258 IWIDAKLKLMVDPLLLIHSLVISENADMAISKHPFY 293
IW D KLK D LL S ++S ADM+++ +Y
Sbjct: 414 IWFDRKLKNTSDNLLFSRSPLLSVYADMSVTCKEYY 449
>CRTI_ARATH (Q07356) Phytoene dehydrogenase, chloroplast precursor
(EC 1.14.99.-) (Phytoene desaturase)
Length = 566
Score = 30.4 bits (67), Expect = 9.8
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 258 IWIDAKLKLMVDPLLLIHSLVISENADMAISKHPFY 293
IW D KLK D LL S ++S ADM+++ +Y
Sbjct: 396 IWFDRKLKNTYDHLLFSRSNLLSVYADMSLTCKEYY 431
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.136 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,196,434
Number of Sequences: 164201
Number of extensions: 2146438
Number of successful extensions: 4729
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 4720
Number of HSP's gapped (non-prelim): 20
length of query: 456
length of database: 59,974,054
effective HSP length: 114
effective length of query: 342
effective length of database: 41,255,140
effective search space: 14109257880
effective search space used: 14109257880
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 67 (30.4 bits)
Lotus: description of TM0265.9