BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0265.3
(532 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ANGR_VIBAN (P19828) Protein angR 36 0.28
SYE1_COXBU (Q83EV3) Glutamyl-tRNA synthetase 1 (EC 6.1.1.17) (Gl... 33 2.4
VML2_HUMAN (Q8NFU0) Bestrophin 3 (Vitelliform macular dystrophy ... 32 5.4
SKEL_DROME (Q9GPJ1) Skeletor protein 32 5.4
NME2_RAT (Q00960) Glutamate [NMDA] receptor subunit epsilon 2 pr... 31 7.0
NME2_MOUSE (Q01097) Glutamate [NMDA] receptor subunit epsilon 2 ... 31 7.0
NME2_HUMAN (Q13224) Glutamate [NMDA] receptor subunit epsilon 2 ... 31 7.0
ERG4_YEAST (P25340) Delta(24(24(1)))-sterol reductase (EC 1.3.1.... 31 9.2
ELK4_MOUSE (P41158) ETS-domain protein Elk-4 (Serum response fac... 31 9.2
CITT_ECOLI (P77405) Citrate carrier (Citrate transporter) (Citra... 31 9.2
ARO1_EMENI (P07547) Pentafunctional AROM polypeptide [Includes: ... 31 9.2
>ANGR_VIBAN (P19828) Protein angR
Length = 1048
Score = 35.8 bits (81), Expect = 0.28
Identities = 19/62 (30%), Positives = 32/62 (50%)
Query: 466 ITKRSVFDTTIGYYNEEELLGLSPFSFTPSFYVTQAFKAWWSAYWHDISTPIEDCFQRIT 525
ITK + + IG + E L+G+S F + V K + AY +D+ P+ C +++
Sbjct: 277 ITKINDYTGIIGQFTEPLLVGMSGFEQSFLSLVKNNQKKFEEAYHYDVKVPVFQCVNKLS 336
Query: 526 NI 527
NI
Sbjct: 337 NI 338
>SYE1_COXBU (Q83EV3) Glutamyl-tRNA synthetase 1 (EC 6.1.1.17)
(Glutamate--tRNA ligase 1) (GluRS 1)
Length = 469
Score = 32.7 bits (73), Expect = 2.4
Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 10/83 (12%)
Query: 134 FGHWKVTGIFDLIQLSRSPITYNPAMLLSSLFFWERSTNSFHVPFGTLSPTLLDVAAITG 193
F ++ +FD+ +SRSP +NP LL W N ++ T+SPT++ A T
Sbjct: 277 FSREEMVQLFDISAVSRSPAAFNPEKLL-----W---LNQHYLK--TVSPTIIAEAFATQ 326
Query: 194 LWVVGDDYHSSAAPTNPIAIPTD 216
L G D + + IA+ +
Sbjct: 327 LEKAGTDLRNGPSLEQVIALQAE 349
>VML2_HUMAN (Q8NFU0) Bestrophin 3 (Vitelliform macular dystrophy
2-like protein 2)
Length = 473
Score = 31.6 bits (70), Expect = 5.4
Identities = 20/55 (36%), Positives = 23/55 (41%), Gaps = 5/55 (9%)
Query: 465 TITKRSVFDTTIGYYNEEELLGLSPFSFTPSFYVTQAFKAWWSAYWHDISTPIED 519
T +R V+ Y N L P SF FYVT WWS Y S P+ D
Sbjct: 55 TQEQRYVYAQVARYCNRSA--DLIPLSFVLGFYVTLVVNRWWSQY---TSIPLPD 104
>SKEL_DROME (Q9GPJ1) Skeletor protein
Length = 743
Score = 31.6 bits (70), Expect = 5.4
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
Query: 44 VFIPISVNGNLRAILGPLKKAKKGRPNSLPEGYEHFHPSIRGDELILAFQPS 95
+F P+ + G R P+KK RP LP+ + H HP + ++L QPS
Sbjct: 200 LFHPVKLPGR-RPFPAPIKKVPASRP-ILPQQHPHLHPHPQQHPVLLQQQPS 249
>NME2_RAT (Q00960) Glutamate [NMDA] receptor subunit epsilon 2
precursor (N-methyl D-aspartate receptor subtype 2B)
(NR2B) (NMDAR2B)
Length = 1482
Score = 31.2 bits (69), Expect = 7.0
Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 3/58 (5%)
Query: 291 NEAIADIRDPKVASLNAAGPFWLLQLWMNAVFEPSLPAKNPPPTVSNTRIEAFRLEAL 348
N +AD R+P S WLL W VF S+P +NP T S + + A+
Sbjct: 586 NRCLADGREPGGPSFTIGKAIWLL--W-GLVFNNSVPVQNPKGTTSKIMVSVWAFFAV 640
>NME2_MOUSE (Q01097) Glutamate [NMDA] receptor subunit epsilon 2
precursor (N-methyl D-aspartate receptor subtype 2B)
(NR2B) (NMDAR2B)
Length = 1482
Score = 31.2 bits (69), Expect = 7.0
Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 3/58 (5%)
Query: 291 NEAIADIRDPKVASLNAAGPFWLLQLWMNAVFEPSLPAKNPPPTVSNTRIEAFRLEAL 348
N +AD R+P S WLL W VF S+P +NP T S + + A+
Sbjct: 586 NRCLADGREPGGPSFTIGKAIWLL--W-GLVFNNSVPVQNPKGTTSKIMVSVWAFFAV 640
>NME2_HUMAN (Q13224) Glutamate [NMDA] receptor subunit epsilon 2
precursor (N-methyl D-aspartate receptor subtype 2B)
(NR2B) (NMDAR2B) (N-methyl-D-aspartate receptor subunit
3) (NR3) (hNR3)
Length = 1484
Score = 31.2 bits (69), Expect = 7.0
Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 3/58 (5%)
Query: 291 NEAIADIRDPKVASLNAAGPFWLLQLWMNAVFEPSLPAKNPPPTVSNTRIEAFRLEAL 348
N +AD R+P S WLL W VF S+P +NP T S + + A+
Sbjct: 586 NRCLADGREPGGPSFTIGKAIWLL--W-GLVFNNSVPVQNPKGTTSKIMVSVWAFFAV 640
>ERG4_YEAST (P25340) Delta(24(24(1)))-sterol reductase (EC 1.3.1.71)
(Sterol delta(24(28))-reductase) (C-24(28) sterol
reductase)
Length = 473
Score = 30.8 bits (68), Expect = 9.2
Identities = 21/79 (26%), Positives = 35/79 (43%), Gaps = 9/79 (11%)
Query: 194 LWVVGDDYHSSAAPTNPIAIPTDNIAFSKFIKDHY---VENGEVSDAEHVAFLLYWLSAY 250
+W+ + YH +A+P ++ FIK Y +ENG + FL +W+
Sbjct: 60 MWICAEFYHGK------VALPKAGESWMHFIKHLYQLVLENGIPEKYDWTIFLTFWVFQI 113
Query: 251 VFCTKSLRIPAKLLPLANL 269
+F I K PL++L
Sbjct: 114 IFYYTLPGIWTKGQPLSHL 132
>ELK4_MOUSE (P41158) ETS-domain protein Elk-4 (Serum response factor
accessory protein 1) (SAP-1)
Length = 430
Score = 30.8 bits (68), Expect = 9.2
Identities = 20/41 (48%), Positives = 24/41 (57%), Gaps = 5/41 (12%)
Query: 149 SRSPITYNPAMLLSSLFFWERSTNSFHVPFGTLSPTLLDVA 189
S++PI P+ LLSS+ FW ST S PF LSP L A
Sbjct: 358 SQTPILLTPSPLLSSIHFW--STLS---PFAPLSPARLQGA 393
>CITT_ECOLI (P77405) Citrate carrier (Citrate transporter)
(Citrate/succinate antiporter)
Length = 487
Score = 30.8 bits (68), Expect = 9.2
Identities = 22/64 (34%), Positives = 29/64 (44%), Gaps = 8/64 (12%)
Query: 86 DELILAFQPSYR-------FPFLKDPKRTFRSAPPNPSAGE-GAYLRWLDRVEASKFGHW 137
D L+ F PS FP +K+ F+S P +PSA G YL W+ + S
Sbjct: 149 DILLAPFTPSNTARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSM 208
Query: 138 KVTG 141
VTG
Sbjct: 209 FVTG 212
>ARO1_EMENI (P07547) Pentafunctional AROM polypeptide [Includes:
3-dehydroquinate synthase (EC 4.2.3.4); 3-dehydroquinate
dehydratase (EC 4.2.1.10) (3-dehydroquinase); Shikimate
dehydrogenase (EC 1.1.1.25); Shikimate kinase (EC
2.7.1.71); 3-phosphoshikima
Length = 1586
Score = 30.8 bits (68), Expect = 9.2
Identities = 34/140 (24%), Positives = 58/140 (41%), Gaps = 22/140 (15%)
Query: 205 AAPTNPIAIPTDNIAFSKFIKDHYVENGEVSDAEHVAFLLYWLSAYVFCTKSLRIPAKLL 264
A P + + T+ FSK I H+ GE+S A + W+ Y K L
Sbjct: 1157 AFPEDMLRAVTEMKGFSKIIASHHDPKGELSWAN-----MSWIKFY----------NKAL 1201
Query: 265 PLANLLHEGRIIAMARLVLGN-LYQMINEAIADIRDPKVASLNAAGPFWLLQL---WMNA 320
+++ +++ +AR + N + A+ D + ++N L ++ +M
Sbjct: 1202 EYGDII---KLVGVARNIDDNTALRKFKNWAAEAHDVPLIAINMGDQGQLSRILNGFMTP 1258
Query: 321 VFEPSLPAKNPPPTVSNTRI 340
V PSLP K P +S T I
Sbjct: 1259 VSHPSLPFKAAPGQLSATEI 1278
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.138 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,741,726
Number of Sequences: 164201
Number of extensions: 2964820
Number of successful extensions: 5769
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 5763
Number of HSP's gapped (non-prelim): 12
length of query: 532
length of database: 59,974,054
effective HSP length: 115
effective length of query: 417
effective length of database: 41,090,939
effective search space: 17134921563
effective search space used: 17134921563
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0265.3