BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0258b.1
(207 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SMR2_RAT (P18897) SMR2 protein precursor 32 1.4
GX42_RAT (Q91XR8) Phospholipid hydroperoxide glutathione peroxid... 31 1.8
BOL_DROME (Q24207) Boule protein 31 1.8
ZN90_HUMAN (Q03938) Zinc finger protein 90 (Zinc finger protein ... 31 2.3
Z275_HUMAN (Q9NSD4) Zinc finger protein 275 31 2.3
MT_ARIAR (P55946) Metallothionein (MT) 31 2.3
Z135_HUMAN (P52742) Zinc finger protein 135 30 3.0
TAT_HV1BR (P04610) TAT protein (Transactivating regulatory protein) 30 3.0
M3K1_HUMAN (Q13233) Mitogen-activated protein kinase kinase kina... 30 3.0
YO91_CAEEL (P41842) Hypothetical protein T20B12.1 in chromosome III 30 4.0
RNP4_HUMAN (Q86U06) RNA-binding region containing protein 4 (Spl... 30 4.0
TAT_HV1PV (P04607) TAT protein (Transactivating regulatory protein) 30 5.2
TAT_HV1B1 (P04606) TAT protein (Transactivating regulatory protein) 30 5.2
PYC2_YEAST (P32327) Pyruvate carboxylase 2 (EC 6.4.1.1) (Pyruvic... 30 5.2
PYC1_YEAST (P11154) Pyruvate carboxylase 1 (EC 6.4.1.1) (Pyruvic... 30 5.2
PAP2_XENLA (P51005) Poly(A) polymerase type 2 (EC 2.7.7.19) (PAP... 30 5.2
DLG5_HUMAN (Q8TDM6) Discs, large homolog 5 (Placenta and prostat... 30 5.2
CIC_DROME (Q9U1H0) Putative transcription factor capicua 30 5.2
YJK3_YEAST (P42950) Putative 70.4 kDa transcriptional regulatory... 29 6.8
PPG1_BOVIN (Q865B7) Peroxisome proliferator activated receptor g... 29 6.8
>SMR2_RAT (P18897) SMR2 protein precursor
Length = 137
Score = 31.6 bits (70), Expect = 1.4
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 4/47 (8%)
Query: 51 PVDRRRHRLMEHQPPPTTNNPHHLHEDIPQSHNQNPFTSLEELKFPE 97
P D+ RHR + PP T ++ +L I + PF SL FPE
Sbjct: 87 PSDKERHRKQKRPPPETLHHRENLRPQIYRQFGIRPFGSL----FPE 129
>GX42_RAT (Q91XR8) Phospholipid hydroperoxide glutathione
peroxidase, nuclear (EC 1.11.1.12) (GPX-4)
Length = 253
Score = 31.2 bits (69), Expect = 1.8
Identities = 15/37 (40%), Positives = 20/37 (53%)
Query: 54 RRRHRLMEHQPPPTTNNPHHLHEDIPQSHNQNPFTSL 90
RRR R +P P NP L +D+PQ+ N + F L
Sbjct: 48 RRRARPRRMEPIPEPFNPRPLLQDLPQTSNSHEFLGL 84
>BOL_DROME (Q24207) Boule protein
Length = 228
Score = 31.2 bits (69), Expect = 1.8
Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 6/51 (11%)
Query: 44 HVRSTWIPVDRRRHRLMEHQPPPTTNNPHHLHEDIPQSHNQNPFTSLEELK 94
+V + W + H + H P T+NPH H QSH Q+P S+E+L+
Sbjct: 178 NVPTIWPQNYQENHSPLLHSP---TSNPHSPHS---QSHPQSPCWSIEDLR 222
>ZN90_HUMAN (Q03938) Zinc finger protein 90 (Zinc finger protein
HTF9) (Fragment)
Length = 195
Score = 30.8 bits (68), Expect = 2.3
Identities = 17/58 (29%), Positives = 29/58 (49%), Gaps = 1/58 (1%)
Query: 10 KGSRCQECGNQAKKGC-AYSRCRTCCNNKGFQCQTHVRSTWIPVDRRRHRLMEHQPPP 66
KG + G K+G ++C T +K FQC T+V+ + I + RH++ + P
Sbjct: 115 KGCESVDEGKVHKRGYNGLNQCLTATQSKVFQCDTYVKVSHIFSNSNRHKIRDTGKKP 172
>Z275_HUMAN (Q9NSD4) Zinc finger protein 275
Length = 458
Score = 30.8 bits (68), Expect = 2.3
Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 1/61 (1%)
Query: 7 QEVKGSRCQECGNQAKKGCAYSR-CRTCCNNKGFQCQTHVRSTWIPVDRRRHRLMEHQPP 65
+E K C+ECG + KK S+ R K F C+ RS + RH+ +
Sbjct: 205 REAKPFECEECGKRFKKNAGLSQHLRVHSREKPFDCEECGRSFKVNTHLFRHQKLHTSEK 264
Query: 66 P 66
P
Sbjct: 265 P 265
>MT_ARIAR (P55946) Metallothionein (MT)
Length = 66
Score = 30.8 bits (68), Expect = 2.3
Identities = 15/32 (46%), Positives = 21/32 (64%), Gaps = 6/32 (18%)
Query: 11 GSRCQECGNQAKKGCAYSRCRTC-CNNKGFQC 41
GS+CQ CG +GCA + C+TC C + G +C
Sbjct: 21 GSKCQ-CG----EGCACASCKTCNCTSDGCKC 47
>Z135_HUMAN (P52742) Zinc finger protein 135
Length = 469
Score = 30.4 bits (67), Expect = 3.0
Identities = 21/78 (26%), Positives = 32/78 (40%), Gaps = 7/78 (8%)
Query: 18 GNQAKKGCAYSRCRT--CCNNKGFQCQTHVRSTWIPVDRRRHRLMEHQPPPTTNNPHHLH 75
GN KKG A +C T C K ++CQ ++ L+EH T P+ H
Sbjct: 2 GNTWKKGEARPKCFTENCVKEKPYKCQECGKAF-----SHSSALIEHHRTHTGERPYECH 56
Query: 76 EDIPQSHNQNPFTSLEEL 93
E + N + T + +
Sbjct: 57 ECLKGFRNSSALTKHQRI 74
>TAT_HV1BR (P04610) TAT protein (Transactivating regulatory
protein)
Length = 86
Score = 30.4 bits (67), Expect = 3.0
Identities = 14/57 (24%), Positives = 24/57 (41%), Gaps = 4/57 (7%)
Query: 18 GNQAKKGCAYSRCRTCCNNKGFQCQTHVRSTWIPVDRRRHRLMEHQPPPTTNNPHHL 74
G+Q K C C+ CC F CQ + + + R + + + PP + H +
Sbjct: 15 GSQPKTACTTCYCKKCC----FHCQVCFTTKALGISYGRKKRRQRRRPPQGSQTHQV 67
>M3K1_HUMAN (Q13233) Mitogen-activated protein kinase kinase kinase
1 (EC 2.7.1.37) (MAPK/ERK kinase kinase 1) (MEK kinase
1) (MEKK 1) (Fragment)
Length = 1495
Score = 30.4 bits (67), Expect = 3.0
Identities = 13/38 (34%), Positives = 20/38 (52%)
Query: 142 PEQQSLNASPLDQHQNLNLTTIHSHDGATMAPPSSSAT 179
P Q LN+SPL H L + + +T + P+ +AT
Sbjct: 945 PHSQCLNSSPLSHHSQLMFPALSTPSSSTPSVPAGTAT 982
>YO91_CAEEL (P41842) Hypothetical protein T20B12.1 in chromosome III
Length = 787
Score = 30.0 bits (66), Expect = 4.0
Identities = 15/36 (41%), Positives = 19/36 (52%)
Query: 62 HQPPPTTNNPHHLHEDIPQSHNQNPFTSLEELKFPE 97
H+ P TT P DIPQS + N T LE++ E
Sbjct: 228 HKDPSTTGTPLPPDSDIPQSLDMNDDTLLEQVAITE 263
>RNP4_HUMAN (Q86U06) RNA-binding region containing protein 4
(Splicing factor SF2) (PP239)
Length = 439
Score = 30.0 bits (66), Expect = 4.0
Identities = 32/138 (23%), Positives = 51/138 (36%), Gaps = 18/138 (13%)
Query: 27 YSRCRTCCNNKGFQCQTHVRSTWI--------PVDRRRHRLMEHQPPPTTNNPHHLHEDI 78
Y R + + G QC+ RS W D RR + ++ PP + H
Sbjct: 84 YRRRNSRSRSPGRQCRHRSRS-WDRRHGSESRSRDHRREDRVHYRSPPLATGYRYGHSKS 142
Query: 79 PQSHNQNPFTSLEELKFPEAMSSMAVF-----SSVRVRSMDDSVNEMAYQTSVNI----G 129
P ++P + PE + VF + +R R ++D + + V I
Sbjct: 143 PHFREKSPVREPVDNLSPEERDARTVFCMQLAARIRPRDLEDFFSAVGKVRDVRIISDRN 202
Query: 130 GHRFSGILYDQGPEQQSL 147
R GI Y + E QS+
Sbjct: 203 SRRSKGIAYVEFCEIQSV 220
>TAT_HV1PV (P04607) TAT protein (Transactivating regulatory
protein)
Length = 86
Score = 29.6 bits (65), Expect = 5.2
Identities = 14/57 (24%), Positives = 24/57 (41%), Gaps = 4/57 (7%)
Query: 18 GNQAKKGCAYSRCRTCCNNKGFQCQTHVRSTWIPVDRRRHRLMEHQPPPTTNNPHHL 74
G+Q K C C+ CC F CQ + + + R + + + PP + H +
Sbjct: 15 GSQPKTACTNCYCKKCC----FHCQVCFITKALGISYGRKKRRQRRRPPQGSQTHQV 67
>TAT_HV1B1 (P04606) TAT protein (Transactivating regulatory
protein)
Length = 86
Score = 29.6 bits (65), Expect = 5.2
Identities = 14/57 (24%), Positives = 24/57 (41%), Gaps = 4/57 (7%)
Query: 18 GNQAKKGCAYSRCRTCCNNKGFQCQTHVRSTWIPVDRRRHRLMEHQPPPTTNNPHHL 74
G+Q K C C+ CC F CQ + + + R + + + PP + H +
Sbjct: 15 GSQPKTACTNCYCKKCC----FHCQVCFITKALGISYGRKKRRQRRRPPQGSQTHQV 67
>PYC2_YEAST (P32327) Pyruvate carboxylase 2 (EC 6.4.1.1) (Pyruvic
carboxylase 2) (PCB 2)
Length = 1180
Score = 29.6 bits (65), Expect = 5.2
Identities = 13/28 (46%), Positives = 19/28 (67%)
Query: 153 DQHQNLNLTTIHSHDGATMAPPSSSATA 180
D HQ+L T + +HD AT+AP ++ A A
Sbjct: 567 DAHQSLLATRVRTHDLATIAPTTAHALA 594
>PYC1_YEAST (P11154) Pyruvate carboxylase 1 (EC 6.4.1.1) (Pyruvic
carboxylase 1) (PCB 1)
Length = 1178
Score = 29.6 bits (65), Expect = 5.2
Identities = 13/28 (46%), Positives = 19/28 (67%)
Query: 153 DQHQNLNLTTIHSHDGATMAPPSSSATA 180
D HQ+L T + +HD AT+AP ++ A A
Sbjct: 566 DAHQSLLATRVRTHDLATIAPTTAHALA 593
>PAP2_XENLA (P51005) Poly(A) polymerase type 2 (EC 2.7.7.19) (PAP)
(Polynucleotide adenylyltransferase)
Length = 484
Score = 29.6 bits (65), Expect = 5.2
Identities = 34/141 (24%), Positives = 63/141 (44%), Gaps = 19/141 (13%)
Query: 59 LMEHQPPPTTNNPHHLHEDIPQSHNQNPFTSLEELKFPEAMSSMAVFSSVRVRSMDDSVN 118
+M Q T N+ L+E IP++ F+S +SSM + + V+ + + ++V
Sbjct: 324 VMVPQNNSTENSGGSLNESIPETATHPAFSSTPRPLVTRVVSSMPLVNQVQ-KPVTNTVT 382
Query: 119 EMAYQTSVNIGGHRFSGILYDQGP--------EQQSLNASPLDQHQNL---NLTTIHSHD 167
+M S G R S ++ P E S N++ +D+ L L +HS +
Sbjct: 383 KM---PSPVAGVKRTSSPTNEESPKKTKTEEDENDSSNSTEVDEQNKLEPEELKEVHSEE 439
Query: 168 GATM----APPSSSATAAHEL 184
++ A PSS +++ +L
Sbjct: 440 KSSSPVPGALPSSQRSSSTDL 460
>DLG5_HUMAN (Q8TDM6) Discs, large homolog 5 (Placenta and prostate
DLG) (Discs large protein P-dlg)
Length = 1809
Score = 29.6 bits (65), Expect = 5.2
Identities = 25/74 (33%), Positives = 30/74 (39%), Gaps = 17/74 (22%)
Query: 141 GPEQQSLNASPLDQHQNLNLTTI----HSHDGATMAPPSSSATAAHELFFPQP------- 189
GPE Q+ N L QH N T I D PP S++ H+ F P
Sbjct: 714 GPEVQAHNKRNLIQHNNSTQTDIFYTDRLEDRKEPGPPGGSSSFLHKPFPGGPLQVCPQA 773
Query: 190 ------RSLASFRS 197
RSL+SFRS
Sbjct: 774 CPSASERSLSSFRS 787
>CIC_DROME (Q9U1H0) Putative transcription factor capicua
Length = 1403
Score = 29.6 bits (65), Expect = 5.2
Identities = 29/121 (23%), Positives = 47/121 (37%), Gaps = 14/121 (11%)
Query: 55 RRHRLMEHQPPPTTNNPHHLHEDIPQSHNQNPFTSLEELKFPEAMSSMAVF--SSVRVRS 112
R H + PPP + P L P SH P K + A + S+ R
Sbjct: 967 RNHNGLSSLPPPVSAQPTMLLHGYPPSHGVEPPARSPSYKSMPSTPKSATYLMSAPPERG 1026
Query: 113 MDDSVNEMAY-------QTSVNIGGHRFSGILYDQGPEQQSLNASPLDQHQNLNLTTIHS 165
MD ++ A ++ ++ G +F IL P L +PL + +NL+ + S
Sbjct: 1027 MDGGMSGCASAAASGGDESDIDADGQQF--IL---APTPAQLGRAPLQRRKNLSQSKSES 1081
Query: 166 H 166
+
Sbjct: 1082 N 1082
>YJK3_YEAST (P42950) Putative 70.4 kDa transcriptional regulatory
protein in IME2-MEF2 intergenic region
Length = 618
Score = 29.3 bits (64), Expect = 6.8
Identities = 22/102 (21%), Positives = 46/102 (44%)
Query: 83 NQNPFTSLEELKFPEAMSSMAVFSSVRVRSMDDSVNEMAYQTSVNIGGHRFSGILYDQGP 142
N + SL+E ++P +S SS+ + ++ +S + Q +IG + YD+
Sbjct: 250 NNSQIFSLQESEYPNPSASTRGNSSLHLTNLLNSTKSVNDQKDSSIGHSTSTFNTYDEVV 309
Query: 143 EQQSLNASPLDQHQNLNLTTIHSHDGATMAPPSSSATAAHEL 184
+ ++ L ++ N T SH+ + SS+ + +L
Sbjct: 310 SRPFISLDMLHLNRGANANTHPSHNAKLESECDSSSHSDADL 351
>PPG1_BOVIN (Q865B7) Peroxisome proliferator activated receptor
gamma coactivator 1 alpha (PPAR gamma coactivator-1
alpha) (PPARGC-1 alpha) (PGC-1 alpha)
Length = 796
Score = 29.3 bits (64), Expect = 6.8
Identities = 35/162 (21%), Positives = 62/162 (37%), Gaps = 26/162 (16%)
Query: 58 RLMEHQPPPTTNNPHHLH-----------EDIPQSHNQNPFTSLEELKFPEAMS------ 100
R +E++ P++N P +H E S +P ++ ++L+ E +
Sbjct: 406 RQLENKDAPSSNGPGQIHSSTDSDPCYLRETAEVSRQVSPGSTRKQLQDQEIRAELNKHF 465
Query: 101 ---SMAVFS-----SVRVRSMDDSVNEMAYQTSVNIGGHRFSGILYDQGPEQQSLNASPL 152
S AVF + +R D S + + G G+ D E LN SP
Sbjct: 466 GHPSQAVFDDKADKTSELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLN-SPW 524
Query: 153 DQHQNLNLTTIHSHDGATMAPPSSSATAAHELFFPQPRSLAS 194
D Q+ +L + + +P S + LF +P+ + S
Sbjct: 525 DGTQSYSLFDVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRS 566
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.129 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,684,818
Number of Sequences: 164201
Number of extensions: 1041315
Number of successful extensions: 2882
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 2868
Number of HSP's gapped (non-prelim): 35
length of query: 207
length of database: 59,974,054
effective HSP length: 105
effective length of query: 102
effective length of database: 42,732,949
effective search space: 4358760798
effective search space used: 4358760798
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0258b.1