BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0252d.2
(519 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YLE1_SCHPO (Q10451) Hypothetical protein C1093.01 in chromosome I 38 0.073
RBSK_YEAST (P25332) Probable ribokinase (EC 2.7.1.15) 33 1.4
STAR_HORSE (O46689) Steroidogenic acute regulatory protein, mito... 33 2.3
STAR_BOVIN (Q28918) Steroidogenic acute regulatory protein, mito... 33 2.3
STAR_SHEEP (P79245) Steroidogenic acute regulatory protein, mito... 32 5.2
POLG_HE71M (Q66479) Genome polyprotein [Contains: Coat protein V... 31 6.8
LUM_MOUSE (P51885) Lumican precursor (Keratan sulfate proteoglyc... 31 6.8
POLG_HE71B (Q66478) Genome polyprotein [Contains: Coat protein V... 31 8.9
CRN_DROME (P17886) Crooked neck protein 31 8.9
>YLE1_SCHPO (Q10451) Hypothetical protein C1093.01 in chromosome I
Length = 1261
Score = 37.7 bits (86), Expect = 0.073
Identities = 21/82 (25%), Positives = 39/82 (46%), Gaps = 5/82 (6%)
Query: 252 LTQGLWVHSYAKRCKVDSNPILATALVDMYSKCGCVESALTVF-----EGIADKDAGAWN 306
+ +G+ + S KR V N + AL+ ++K G + A F EG++ K+ +
Sbjct: 1126 IVEGIQIVSDMKRYNVSLNAYIVNALIKGFTKVGMISKARYYFDLLECEGMSGKEPSTYE 1185
Query: 307 AMISGVALSGDVRKSLELFHQM 328
M+ D RK++E+ Q+
Sbjct: 1186 NMVRAYLSVNDGRKAMEIVEQL 1207
Score = 34.3 bits (77), Expect = 0.80
Identities = 28/125 (22%), Positives = 52/125 (41%), Gaps = 2/125 (1%)
Query: 305 WNAMISGVALSGDVRKSLELFHQMAAHGTQPTETTFVAVLTACTHGGMVHQGLRLFEEMS 364
+NA++S + + + +LF +M G PT T+ V+ A G +LF EM
Sbjct: 929 YNAVLSKLGRARRTTECWKLFQEMKESGLLPTSVTYGTVINAACRIGDESLAEKLFAEME 988
Query: 365 CTYGVEPRGEHYACVVDLLSRAGMVKEAEKFM--EKMGGLVEADLNVWGAILNACRIYKN 422
+PR Y ++ + +E F +E + + +++A K
Sbjct: 989 NQPNYQPRVAPYNTMIQFEVQTMFNREKALFYYNRLCATDIEPSSHTYKLLMDAYGTLKP 1048
Query: 423 VDVGN 427
V+VG+
Sbjct: 1049 VNVGS 1053
Score = 33.9 bits (76), Expect = 1.0
Identities = 22/83 (26%), Positives = 36/83 (42%), Gaps = 1/83 (1%)
Query: 317 DVRKSLELFHQMAAHGTQPTETTFVAVLTACTHGGMVHQGLRLFEEMSCTYGVEPRGEHY 376
D +L +F + H +P+ + AVL+ + +LF+EM G+ P Y
Sbjct: 906 DATTALNIFEETKRHNVKPSVFLYNAVLSKLGRARRTTECWKLFQEMK-ESGLLPTSVTY 964
Query: 377 ACVVDLLSRAGMVKEAEKFMEKM 399
V++ R G AEK +M
Sbjct: 965 GTVINAACRIGDESLAEKLFAEM 987
Score = 33.1 bits (74), Expect = 1.8
Identities = 19/74 (25%), Positives = 32/74 (42%), Gaps = 4/74 (5%)
Query: 181 GKVGDVESARELFEEMPERNAVS----WSAMMAAYSRVSDFKQVLALFEEMQNRGMKPND 236
G D +A +FEE N ++A+++ R + LF+EM+ G+ P
Sbjct: 902 GDTDDATTALNIFEETKRHNVKPSVFLYNAVLSKLGRARRTTECWKLFQEMKESGLLPTS 961
Query: 237 SVLVTVLTACAHLG 250
TV+ A +G
Sbjct: 962 VTYGTVINAACRIG 975
>RBSK_YEAST (P25332) Probable ribokinase (EC 2.7.1.15)
Length = 333
Score = 33.5 bits (75), Expect = 1.4
Identities = 36/141 (25%), Positives = 49/141 (34%), Gaps = 16/141 (11%)
Query: 381 DLLSRAGMVKEAEKFMEKMGGLVEADLNVWGAILNACRIYKNV---DVGNRVWKKMIDKG 437
D L AG A F GG G + N Y +VGN + K +
Sbjct: 18 DRLPNAGETFRANHFETHAGGKGLNQAAAIGKLKNPSSRYSVRMIGNVGNDTFGKQLKDT 77
Query: 438 VADCG---THILTYN----------MYREAGWDAEAKRVRSMVSEAGMKKKPGCSIIEVG 484
++DCG TH+ TY + +AG V S+ K C I G
Sbjct: 78 LSDCGVDITHVGTYEGINTGTATILIEEKAGGQNRILIVEGANSKTIYDPKQLCEIFPEG 137
Query: 485 DEVEEFLAGDHSHPQAQELCK 505
E EE++ H P + K
Sbjct: 138 KEEEEYVVFQHEIPDPLSIIK 158
>STAR_HORSE (O46689) Steroidogenic acute regulatory protein,
mitochondrial precursor (StAR) (StARD1)
Length = 285
Score = 32.7 bits (73), Expect = 2.3
Identities = 39/141 (27%), Positives = 55/141 (38%), Gaps = 20/141 (14%)
Query: 303 GAWNAMISGVALSGDVRKSLELFHQMAAH--GTQPTETTFVAVLTA------CTHGGMVH 354
G WN + + + + K + H++AA G FV+V A C GM
Sbjct: 145 GEWNPNVKEIKVLQKIGKDTVITHELAAESAGNLVGPRDFVSVRCAKRRGSTCVLAGMAT 204
Query: 355 QGLRLFEEMSCTYGVEPRGEHYACVVDLLSRAGMVKEAEKFMEKMGGLVEADLNVW--GA 412
Q FEEM GV R EH + L AG + K+ L+ DL W
Sbjct: 205 Q----FEEMPEQKGV-IRAEHGPTCMVLHPLAGSPSKT-----KLTWLLSIDLKGWLPKT 254
Query: 413 ILNACRIYKNVDVGNRVWKKM 433
I+N VD N + K++
Sbjct: 255 IINQVLSQTQVDFANHLRKRL 275
>STAR_BOVIN (Q28918) Steroidogenic acute regulatory protein,
mitochondrial precursor (StAR) (StARD1)
Length = 285
Score = 32.7 bits (73), Expect = 2.3
Identities = 38/137 (27%), Positives = 55/137 (39%), Gaps = 12/137 (8%)
Query: 303 GAWNAMISGVALSGDVRKSLELFHQMAAH--GTQPTETTFVAVLTACTHGGM-VHQGL-R 358
G WN + + + + K + H++AA G FV+V G M V G+
Sbjct: 145 GEWNPNVKEIKVLQKIGKDTVITHELAAEVAGNLVGPRDFVSVRCTKRRGSMCVLAGMAT 204
Query: 359 LFEEMSCTYGVEPRGEHYACVVDLLSRAGMVKEAEKFMEKMGGLVEADLNVW--GAILNA 416
L+EEM GV R EH + L AG K+ L+ DL W I+N
Sbjct: 205 LYEEMPQQKGV-IRAEHGPTCMVLRPLAGSPSRT-----KLTWLLSIDLKGWLPKTIINQ 258
Query: 417 CRIYKNVDVGNRVWKKM 433
VD N + K++
Sbjct: 259 VLSQTQVDFANHLRKRL 275
>STAR_SHEEP (P79245) Steroidogenic acute regulatory protein,
mitochondrial precursor (StAR) (StARD1)
Length = 285
Score = 31.6 bits (70), Expect = 5.2
Identities = 36/137 (26%), Positives = 53/137 (38%), Gaps = 12/137 (8%)
Query: 303 GAWNAMISGVALSGDVRKSLELFHQMAAH--GTQPTETTFVAVLTACTHGGM--VHQGLR 358
G WN + + + + K + H++AA G FV+V G M +
Sbjct: 145 GEWNPSVKEIKVLQKIGKDTIITHELAAEAAGNLVGPRDFVSVRCTKRRGSMCVLAGTAT 204
Query: 359 LFEEMSCTYGVEPRGEHYACVVDLLSRAGMVKEAEKFMEKMGGLVEADLNVW--GAILNA 416
L+EEM GV R EH + L AG K+ L+ DL W I+N
Sbjct: 205 LYEEMPQQKGV-IRAEHGPTCMVLRPLAGSPSRT-----KLTWLLSIDLKGWLPKTIINQ 258
Query: 417 CRIYKNVDVGNRVWKKM 433
VD N + K++
Sbjct: 259 VLSQTQVDFANHLRKRL 275
>POLG_HE71M (Q66479) Genome polyprotein [Contains: Coat protein VP4
(P1A); Coat protein VP2 (P1B); Coat protein VP3 (P1C);
Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29)
(Core protein P2A); Core protein P2B; Core protein P2C;
Core protein P3A; Genome
Length = 2193
Score = 31.2 bits (69), Expect = 6.8
Identities = 29/91 (31%), Positives = 41/91 (44%), Gaps = 12/91 (13%)
Query: 264 RCKVDSNPILATALVDMYSKCGCVESALTVFEGIADKDAGA-WNAMISGVALSGDVRKSL 322
RC ++S+ T L +S+ G V EG + + A W+ I+G A +R+ +
Sbjct: 632 RCVLNSHSTAETTLDSFFSRAGLVGEIDLPLEGTTNPNGYANWDIDITGYA---QMRRKV 688
Query: 323 ELFHQMAAHGTQPTETTFVAVLTACTHGGMV 353
ELF M E TFV ACT G V
Sbjct: 689 ELFTYMRF----DAEFTFV----ACTPTGEV 711
>LUM_MOUSE (P51885) Lumican precursor (Keratan sulfate proteoglycan
lumican) (KSPG lumican)
Length = 338
Score = 31.2 bits (69), Expect = 6.8
Identities = 27/107 (25%), Positives = 52/107 (48%), Gaps = 7/107 (6%)
Query: 188 SARELFEEMPERNAVSWSAMMAAYSRVSDFKQVLALFEEMQNRGMKPNDSVLVTVLTACA 247
S EL + N+ + S+++ + K + + E ++N ++ N+ V + C
Sbjct: 237 SHNELADSGVPGNSFNISSLLELDLSYNKLKSIPTVNENLENYYLEVNELEKFDVKSFCK 296
Query: 248 HLGALTQGLWVHSYAKRCKVDSNPILATAL-VDMYSKCGCVESALTV 293
LG L+ +S K ++D NP+ ++L DMY +C V + +TV
Sbjct: 297 ILGPLS-----YSKIKHLRLDGNPLTQSSLPPDMY-ECLRVANEITV 337
>POLG_HE71B (Q66478) Genome polyprotein [Contains: Coat protein VP4
(P1A); Coat protein VP2 (P1B); Coat protein VP3 (P1C);
Coat protein VP1 (P1D); Picornain 2A (EC 3.4.22.29)
(Core protein P2A); Core protein P2B; Core protein P2C;
Core protein P3A; Genome
Length = 2193
Score = 30.8 bits (68), Expect = 8.9
Identities = 31/108 (28%), Positives = 46/108 (41%), Gaps = 12/108 (11%)
Query: 247 AHLGALTQGLWVHSYAKRCKVDSNPILATALVDMYSKCGCVESALTVFEGIADKDAGA-W 305
A +GA + RC ++S+ T L +S+ G V +G + + A W
Sbjct: 615 AEIGASSNASDESMIETRCVLNSHSTAETTLDSFFSRAGLVGEIDLPLKGTTNPNGYANW 674
Query: 306 NAMISGVALSGDVRKSLELFHQMAAHGTQPTETTFVAVLTACTHGGMV 353
+ I+G A +R+ +ELF M E TFV ACT G V
Sbjct: 675 DIDITGYA---QMRRKVELFTYMRF----DAEFTFV----ACTPTGRV 711
>CRN_DROME (P17886) Crooked neck protein
Length = 702
Score = 30.8 bits (68), Expect = 8.9
Identities = 22/98 (22%), Positives = 44/98 (44%), Gaps = 14/98 (14%)
Query: 144 GFIEFYCASRQLQTARLLFDQTTE---KDVVLWTAMIDGYGKVGDVESARELFE------ 194
G+I+ R+ + R+L+++ E ++ V W + +GD + AR +FE
Sbjct: 445 GYIDLEIQLREFERCRMLYEKFLEFGPENCVTWMKFAELENLLGDTDRARAIFELAVQQP 504
Query: 195 --EMPERNAVSWSAMMAAYSRVSDFKQVLALFEEMQNR 230
+MPE + W A + + + + L+E + R
Sbjct: 505 RLDMPE---LLWKAYIDFEVALGETELARQLYERLLER 539
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.133 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,043,288
Number of Sequences: 164201
Number of extensions: 2317680
Number of successful extensions: 5865
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 5858
Number of HSP's gapped (non-prelim): 15
length of query: 519
length of database: 59,974,054
effective HSP length: 115
effective length of query: 404
effective length of database: 41,090,939
effective search space: 16600739356
effective search space used: 16600739356
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0252d.2