BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0252c.1
(325 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
HEXP_LEIMA (Q04832) DNA-binding protein HEXBP (Hexamer-binding p... 43 0.001
CPSD_HUMAN (O95639) Cleavage and polyadenylation specificity fac... 43 0.001
CPSD_MOUSE (Q8BQZ5) Cleavage and polyadenylation specificity fac... 40 0.010
GR2B_ARATH (Q38896) Glycine-rich protein 2b (AtGRP2b) 37 0.051
COAT_SOCMV (P15627) Coat protein 37 0.067
GLH2_CAEEL (Q966L9) ATP-dependent RNA helicase glh-2 (EC 3.6.1.-... 36 0.11
GRP2_NICSY (P27484) Glycine-rich protein 2 34 0.44
GAG_SIVAI (Q02843) Gag polyprotein [Contains: Core protein p17; ... 34 0.44
GAG_HV1MA (P04594) Gag polyprotein [Contains: Core protein p17 (... 34 0.57
GLH1_CAEEL (P34689) ATP-dependent RNA helicase glh-1 (EC 3.6.1.-... 33 0.97
RSG1_RAT (P50904) Ras GTPase-activating protein 1 (GTPase-activa... 33 1.3
CNBP_CHICK (O42395) Cellular nucleic acid binding protein (CNBP) 32 1.7
COAT_CAMVC (P03543) Coat protein 32 2.2
CNBP_RAT (P62634) Cellular nucleic acid binding protein (CNBP) (... 32 2.2
CNBP_MOUSE (P53996) Cellular nucleic acid binding protein (CNBP)... 32 2.2
CNBP_HUMAN (P62633) Cellular nucleic acid binding protein (CNBP)... 32 2.2
GAG_RSVP (P03322) Gag polyprotein [Contains: Core protein p19; C... 32 2.8
GAG_MMTVC (P11284) Gag polyprotein [Contains: Protein p10; Phosp... 32 2.8
COAT_CAMVD (P03544) Coat protein 32 2.8
LONM_YEAST (P36775) Lon protease homolog, mitochondrial precurso... 31 3.7
>HEXP_LEIMA (Q04832) DNA-binding protein HEXBP (Hexamer-binding
protein)
Length = 271
Score = 42.7 bits (99), Expect = 0.001
Identities = 19/49 (38%), Positives = 27/49 (54%), Gaps = 2/49 (4%)
Query: 277 PTGEGFTPGLYRPTIAAAGGAGSQAGSREITCFKCGEIGHYSTKCPKGK 325
P+ +G + G Y +G G +G R TC+KCG+ GH S CP G+
Sbjct: 113 PSSQGGSRGGYGQKRGRSGAQGGYSGDR--TCYKCGDAGHISRDCPNGQ 159
Score = 40.8 bits (94), Expect = 0.005
Identities = 24/65 (36%), Positives = 29/65 (43%), Gaps = 8/65 (12%)
Query: 266 GKLQRKKPYQRPTGEGFT-------PG-LYRPTIAAAGGAGSQAGSREITCFKCGEIGHY 317
G + R+ P TG G PG + R A G G G + TC+KCGE GH
Sbjct: 205 GHMSRECPSAGSTGSGDRACYKCGKPGHISRECPEAGGSYGGSRGGGDRTCYKCGEAGHI 264
Query: 318 STKCP 322
S CP
Sbjct: 265 SRDCP 269
Score = 39.7 bits (91), Expect = 0.010
Identities = 14/30 (46%), Positives = 20/30 (66%)
Query: 296 GAGSQAGSREITCFKCGEIGHYSTKCPKGK 325
G G +G+ + TC+KCG+ GH S CP G+
Sbjct: 158 GQGGYSGAGDRTCYKCGDAGHISRDCPNGQ 187
Score = 36.6 bits (83), Expect = 0.088
Identities = 14/26 (53%), Positives = 17/26 (64%)
Query: 297 AGSQAGSREITCFKCGEIGHYSTKCP 322
A S+ R TCF+CGE GH S +CP
Sbjct: 34 ADSKGDERSTTCFRCGEEGHMSRECP 59
Score = 36.2 bits (82), Expect = 0.11
Identities = 24/77 (31%), Positives = 34/77 (43%), Gaps = 17/77 (22%)
Query: 257 TSSRSYQGKGKLQRKKPYQRPTGEGFTPGLYR-----------PTIAAAGGAGSQAGSRE 305
TS R+ +G R+ P G+ + +R P A +G AG+
Sbjct: 16 TSCRNCGKEGHYARECPEADSKGDERSTTCFRCGEEGHMSRECPNEARSGAAGA------ 69
Query: 306 ITCFKCGEIGHYSTKCP 322
+TCF+CGE GH S CP
Sbjct: 70 MTCFRCGEAGHMSRDCP 86
Score = 36.2 bits (82), Expect = 0.11
Identities = 13/27 (48%), Positives = 18/27 (66%)
Query: 296 GAGSQAGSREITCFKCGEIGHYSTKCP 322
G G +G+ + C+KCGE GH S +CP
Sbjct: 186 GQGGYSGAGDRKCYKCGESGHMSRECP 212
Score = 32.7 bits (73), Expect = 1.3
Identities = 14/27 (51%), Positives = 19/27 (69%), Gaps = 1/27 (3%)
Query: 297 AGSQAGSREITCFKCGEIGHYSTKCPK 323
AGS GS + C+KCG+ GH S +CP+
Sbjct: 214 AGS-TGSGDRACYKCGKPGHISRECPE 239
>CPSD_HUMAN (O95639) Cleavage and polyadenylation specificity
factor, 30 kDa subunit (CPSF 30 kDa subunit) (NS1
effector domain-binding protein 1) (Neb-1) (No arches
homolog)
Length = 269
Score = 42.7 bits (99), Expect = 0.001
Identities = 26/80 (32%), Positives = 37/80 (45%), Gaps = 9/80 (11%)
Query: 254 PMRTSSRSYQGKGK-LQRKKPYQRPTGEGFTPGLYRP--TIAAAGGAGSQAGSR------ 304
P +T + Q LQR + T + +P R I S AG+R
Sbjct: 182 PQQTQPPAKQSNNPPLQRSSSLIQLTSQNSSPNQQRTPQVIGVMQSQNSSAGNRGPRPLE 241
Query: 305 EITCFKCGEIGHYSTKCPKG 324
++TC+KCGE GHY+ +C KG
Sbjct: 242 QVTCYKCGEKGHYANRCTKG 261
>CPSD_MOUSE (Q8BQZ5) Cleavage and polyadenylation specificity
factor, 30 kDa subunit (CPSF 30 kDa subunit) (Clipper
homolog) (Clipper/CPSF 30K)
Length = 211
Score = 39.7 bits (91), Expect = 0.010
Identities = 16/40 (40%), Positives = 26/40 (65%), Gaps = 1/40 (2%)
Query: 285 GLYRPTIAAAGGAGSQAGSREITCFKCGEIGHYSTKCPKG 324
G+ + ++AG G + ++TC+KCGE GHY+ +C KG
Sbjct: 165 GVMQSQNSSAGNRGPRP-LEQVTCYKCGEKGHYANRCTKG 203
>GR2B_ARATH (Q38896) Glycine-rich protein 2b (AtGRP2b)
Length = 201
Score = 37.4 bits (85), Expect = 0.051
Identities = 17/46 (36%), Positives = 22/46 (46%)
Query: 279 GEGFTPGLYRPTIAAAGGAGSQAGSREITCFKCGEIGHYSTKCPKG 324
G G G Y G G G + +CFKCGE GH + +C +G
Sbjct: 109 GGGRGGGSYGGGYGGRGSGGRGGGGGDNSCFKCGEPGHMARECSQG 154
Score = 34.7 bits (78), Expect = 0.33
Identities = 15/46 (32%), Positives = 23/46 (49%), Gaps = 2/46 (4%)
Query: 279 GEGFTPGLYRPTIAAAGGAGSQAGSREITCFKCGEIGHYSTKCPKG 324
G G++ G + GG G G ++C+ CGE GH++ C G
Sbjct: 155 GGGYSGGGGGGRYGSGGGGGGGGGG--LSCYSCGESGHFARDCTSG 198
>COAT_SOCMV (P15627) Coat protein
Length = 441
Score = 37.0 bits (84), Expect = 0.067
Identities = 24/99 (24%), Positives = 44/99 (44%), Gaps = 4/99 (4%)
Query: 229 QALVEKATEVELMKNRRMDRAGTGGPMRTSSRSYQGKGKLQRKKPYQRPTGEGFTPGLYR 288
Q L ++ V L ++ D+ G G +++ R+ + K K ++ K Y + + +
Sbjct: 300 QKLAKRQASVGLCCSKIEDKIGKYGCRKSNPRAKKPKKKFRKIKKYPKKNFWKWNNQRKK 359
Query: 289 PTIAAAGGAGSQ----AGSREITCFKCGEIGHYSTKCPK 323
T Q G ++ C+ C E GHY+ +CPK
Sbjct: 360 KTFRKKRPFRKQQTCPTGKKKCQCWLCHEEGHYANECPK 398
>GLH2_CAEEL (Q966L9) ATP-dependent RNA helicase glh-2 (EC 3.6.1.-)
(Germline helicase-2)
Length = 974
Score = 36.2 bits (82), Expect = 0.11
Identities = 14/32 (43%), Positives = 17/32 (52%)
Query: 294 AGGAGSQAGSREITCFKCGEIGHYSTKCPKGK 325
+GG G G R CF C + GH S CP+ K
Sbjct: 359 SGGGGQDRGERNNNCFNCQQPGHRSNDCPEPK 390
Score = 36.2 bits (82), Expect = 0.11
Identities = 14/32 (43%), Positives = 17/32 (52%)
Query: 294 AGGAGSQAGSREITCFKCGEIGHYSTKCPKGK 325
+GG G G R CF C + GH S CP+ K
Sbjct: 245 SGGGGQDRGERNNNCFNCQQPGHRSNDCPEPK 276
>GRP2_NICSY (P27484) Glycine-rich protein 2
Length = 214
Score = 34.3 bits (77), Expect = 0.44
Identities = 15/27 (55%), Positives = 17/27 (62%), Gaps = 3/27 (11%)
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKC 321
GG GS GS CFKCGE GH++ C
Sbjct: 149 GGGGSGGGSG---CFKCGESGHFARDC 172
Score = 32.7 bits (73), Expect = 1.3
Identities = 13/30 (43%), Positives = 17/30 (56%), Gaps = 4/30 (13%)
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKCPKG 324
GG G G C+KCGE GH++ +C G
Sbjct: 187 GGGGGGGGG----CYKCGEDGHFARECTSG 212
>GAG_SIVAI (Q02843) Gag polyprotein [Contains: Core protein p17;
Core protein p24; Core protein p15]
Length = 513
Score = 34.3 bits (77), Expect = 0.44
Identities = 12/22 (54%), Positives = 16/22 (72%)
Query: 304 REITCFKCGEIGHYSTKCPKGK 325
R+I CFKCG+IGH + C G+
Sbjct: 409 RQIKCFKCGKIGHMAKDCKNGQ 430
>GAG_HV1MA (P04594) Gag polyprotein [Contains: Core protein p17
(Matrix protein); Core protein p24 (Core antigen); Core
protein p2; Core protein p7 (Nucleocapsid protein); Core
protein p1; Core protein p6]
Length = 504
Score = 33.9 bits (76), Expect = 0.57
Identities = 36/133 (27%), Positives = 48/133 (36%), Gaps = 26/133 (19%)
Query: 195 FRDQVDEPYMCKRFVRGLRADIEDSVRPLGIMRFQALVEKATEVELMKNRRMD------R 248
FRD VD RF + LRA+ Q + TE L++N D
Sbjct: 298 FRDYVD------RFFKTLRAE----------QATQEVKNWMTETLLVQNANPDCKTILKA 341
Query: 249 AGTGGPMRTSSRSYQGKGKLQRKKPYQRPTGEGFTPGLYRPTIAAAGGAGSQAGSREITC 308
G G + + QG G K R E + T A G+ G + I C
Sbjct: 342 LGPGATLEEMMTACQGVGGPSHKA---RVLAEAMSQAT-NSTAAIMMQRGNFKGQKRIKC 397
Query: 309 FKCGEIGHYSTKC 321
F CG+ GH + C
Sbjct: 398 FNCGKEGHLARNC 410
>GLH1_CAEEL (P34689) ATP-dependent RNA helicase glh-1 (EC 3.6.1.-)
(Germline helicase-1)
Length = 763
Score = 33.1 bits (74), Expect = 0.97
Identities = 16/46 (34%), Positives = 21/46 (44%)
Query: 278 TGEGFTPGLYRPTIAAAGGAGSQAGSREITCFKCGEIGHYSTKCPK 323
TG G G + + G G G R CF C + GH S+ CP+
Sbjct: 130 TGFGSGGGSFGGGNSGFGEGGHGGGERNNNCFNCQQPGHRSSDCPE 175
>RSG1_RAT (P50904) Ras GTPase-activating protein 1
(GTPase-activating protein) (GAP) (Ras p21 protein
activator) (p120GAP) (RasGAP)
Length = 1038
Score = 32.7 bits (73), Expect = 1.3
Identities = 21/55 (38%), Positives = 27/55 (48%), Gaps = 2/55 (3%)
Query: 249 AGTGGPMRTSSRSYQGKGKLQRKKPYQRPTGEGFTPGLYRPTIAAAGGAGSQAGS 303
AGTGG T S +Y ++ K P P PGL +AAA G G+ +GS
Sbjct: 16 AGTGGAAATGSSAYPAACRV--KLPAAPPMAVAPCPGLADTDLAAALGGGAASGS 68
>CNBP_CHICK (O42395) Cellular nucleic acid binding protein (CNBP)
Length = 172
Score = 32.3 bits (72), Expect = 1.7
Identities = 10/17 (58%), Positives = 13/17 (75%)
Query: 308 CFKCGEIGHYSTKCPKG 324
CFKCG GH++ +CP G
Sbjct: 6 CFKCGRTGHWARECPTG 22
>COAT_CAMVC (P03543) Coat protein
Length = 488
Score = 32.0 bits (71), Expect = 2.2
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Query: 253 GPMRTSSRSYQGKGKLQRK--KPYQRPTGEGFTPGLYRPTIAAAGGAGSQA--GSREITC 308
G +TS + Y + K + K KPY++ + F G Y G G ++ C
Sbjct: 355 GGKKTSKKKYHKRYKKRYKVYKPYKKK--KKFRSGKYFKPKEKKGSKRKYCPKGKKDCRC 412
Query: 309 FKCGEIGHYSTKCP 322
+ C GHY+ +CP
Sbjct: 413 WICNIEGHYANECP 426
>CNBP_RAT (P62634) Cellular nucleic acid binding protein (CNBP)
(Zinc finger protein 9)
Length = 177
Score = 32.0 bits (71), Expect = 2.2
Identities = 10/17 (58%), Positives = 13/17 (75%)
Query: 308 CFKCGEIGHYSTKCPKG 324
CFKCG GH++ +CP G
Sbjct: 6 CFKCGRSGHWARECPTG 22
>CNBP_MOUSE (P53996) Cellular nucleic acid binding protein (CNBP)
(Zinc finger protein 9)
Length = 178
Score = 32.0 bits (71), Expect = 2.2
Identities = 10/17 (58%), Positives = 13/17 (75%)
Query: 308 CFKCGEIGHYSTKCPKG 324
CFKCG GH++ +CP G
Sbjct: 6 CFKCGRSGHWARECPTG 22
>CNBP_HUMAN (P62633) Cellular nucleic acid binding protein (CNBP)
(Zinc finger protein 9)
Length = 177
Score = 32.0 bits (71), Expect = 2.2
Identities = 10/17 (58%), Positives = 13/17 (75%)
Query: 308 CFKCGEIGHYSTKCPKG 324
CFKCG GH++ +CP G
Sbjct: 6 CFKCGRSGHWARECPTG 22
>GAG_RSVP (P03322) Gag polyprotein [Contains: Core protein p19; Core
protein p2A; Core protein p2B; Core protein p10; Capsid
protein p27; Inner coat protein p12; Protease p15 (EC
3.4.23.-)]
Length = 701
Score = 31.6 bits (70), Expect = 2.8
Identities = 13/29 (44%), Positives = 17/29 (57%), Gaps = 1/29 (3%)
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKCPK 323
G GS +R + C+ CG GHY +CPK
Sbjct: 497 GQTGSGGRARGL-CYTCGSPGHYQAQCPK 524
>GAG_MMTVC (P11284) Gag polyprotein [Contains: Protein p10;
Phosphorylated protein pp21; Protein p3; Protein p8;
Major core protein p27; Nucleic acid binding protein
p14]
Length = 591
Score = 31.6 bits (70), Expect = 2.8
Identities = 14/31 (45%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKCPKGK 325
GG G Q GS+ CF CG+ GH C + K
Sbjct: 515 GGKGGQ-GSKGPVCFSCGKTGHIKRDCKEEK 544
>COAT_CAMVD (P03544) Coat protein
Length = 490
Score = 31.6 bits (70), Expect = 2.8
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Query: 253 GPMRTSSRSYQGKGKLQRK--KPYQRPTGEGFTPGLYRPTIAAAGGAGSQA--GSREITC 308
G +TS + Y + K + K KPY++ + F G Y G G ++ C
Sbjct: 356 GCKKTSKKKYHKRYKKKYKAYKPYKKK--KKFRSGKYFKPKEKKGSKQKYCPKGKKDCRC 413
Query: 309 FKCGEIGHYSTKCP 322
+ C GHY+ +CP
Sbjct: 414 WICNIEGHYANECP 427
>LONM_YEAST (P36775) Lon protease homolog, mitochondrial precursor
(EC 3.4.21.-)
Length = 1133
Score = 31.2 bits (69), Expect = 3.7
Identities = 22/63 (34%), Positives = 30/63 (46%), Gaps = 5/63 (7%)
Query: 215 DIEDSVRPLGIMRFQALVEKATEVELMKNRRMDRAGTGGPMRTSSRSYQGKGKLQRKKPY 274
D ++ V P R +V K E E KN R + +GG +SSRS G G ++K P
Sbjct: 119 DTDNDVEPT---RDDEIVNKDQEGEASKNSRS--SASGGGQSSSSRSDSGDGSSKQKPPK 173
Query: 275 QRP 277
P
Sbjct: 174 DVP 176
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.136 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,199,007
Number of Sequences: 164201
Number of extensions: 1656960
Number of successful extensions: 4255
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 4174
Number of HSP's gapped (non-prelim): 104
length of query: 325
length of database: 59,974,054
effective HSP length: 110
effective length of query: 215
effective length of database: 41,911,944
effective search space: 9011067960
effective search space used: 9011067960
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0252c.1