BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0239.10
(553 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MOC_ORYSA (Q84MM9) Protein MONOCULM 1 83 2e-15
DOA_DROME (P49762) Serine/threonine-protein kinase Doa (EC 2.7.1... 36 0.23
DRPL_HUMAN (P54259) Atrophin-1 (Dentatorubral-pallidoluysian atr... 35 0.39
DSH_DROME (P51140) Segment polarity protein dishevelled 34 1.1
SR72_SCHPO (O59787) Signal recognition particle 72 kDa protein h... 33 1.9
PUR2_PYRFU (Q8U3N5) Phosphoribosylamine--glycine ligase (EC 6.3.... 33 1.9
HUNB_DROSL (O46256) Hunchback protein (Fragments) 32 3.3
PK2_DICDI (P28178) Protein kinase 2 (EC 2.7.1.-) 32 4.3
DGAL_CITFR (P23925) D-galactose-binding periplasmic protein prec... 32 4.3
USPA_HAEIN (P44880) Universal stress protein A homolog 32 5.6
KEN_DROME (O77459) Probable transcription factor Ken (Ken and Ba... 32 5.6
KCN3_HUMAN (Q9UGI6) Small conductance calcium-activated potassiu... 32 5.6
HUNB_DROAD (O46232) Hunchback protein (Fragments) 32 5.6
DX_DROME (Q23985) Deltex protein 32 5.6
REPE_ONYPE (P60470) Geminivirus-like replication protein 31 7.4
SRY_MOUSE (Q05738) Sex-determining region Y protein (Testis-dete... 31 9.6
INVO_GALCR (P24710) Involucrin 31 9.6
DGAL_ECOLI (P02927) D-galactose-binding periplasmic protein prec... 31 9.6
>MOC_ORYSA (Q84MM9) Protein MONOCULM 1
Length = 441
Score = 83.2 bits (204), Expect = 2e-15
Identities = 88/369 (23%), Positives = 153/369 (40%), Gaps = 48/369 (13%)
Query: 215 GSAVQRVIFHFAQALQERINKENG---TTLKGSDERVKERHLIELLEVDTNITILCHQKM 271
G A R+ +HFA+AL R++ + G + G R + + +Q
Sbjct: 85 GDAADRLAYHFARALALRVDAKAGHGHVVVGGGAAR----------PASSGAYLAFNQIA 134
Query: 272 PFYQVMQFAGVQAILENVASGTKIHLININIGCGVMCTGLMQALSERQE---NPVEILKI 328
PF + QAILE V ++H+++++ GV L+QA++ER + P E+ ++
Sbjct: 135 PFLRFAHLTANQAILEAVDGARRVHILDLDAVHGVQWPPLLQAIAERADPALGPPEV-RV 193
Query: 329 TAVGVANKTKIEETGKRLMSFAESLNLPFSYNMIFVT-------------------DMME 369
T G A++ + TG RL +FA S++LPF + + ++
Sbjct: 194 TGAG-ADRDTLLRTGNRLRAFARSIHLPFHFTPLLLSCATTAPHHVAGTSTGAAAAASTA 252
Query: 370 IKVEQFDVEDDEAVAVYSPYFLRSLVSDSDSLENLMKVLRKIKPNIMINLGVEA------ 423
+ DE +AV FL +L + D L +K ++ + P ++ EA
Sbjct: 253 AAATGLEFHPDETLAVNCVMFLHNL-AGHDELAAFLKWVKAMSPAVVTIAEREAGGGGGG 311
Query: 424 NHNSPSFVNRFVEALFYYSAYFDCLDTCMKEEHECRMRIE-EMLSEKIRNIVAMEDRERT 482
+ R A+ +YSA F+ L+ + R+ +E E+L +I V R
Sbjct: 312 GDHIDDLPRRVGVAMDHYSAVFEALEATVPPGSRERLAVEQEVLGREIEAAVG-PSGGRW 370
Query: 483 VRSVKIDVWRRFFARYRMVETRLSESAVYQANLVAKKFACGNSCTIDKNGKCLLIGWKGT 542
R I+ W LS AV QA L+ + + + +GW+
Sbjct: 371 WRG--IERWGGAARAAGFAARPLSAFAVSQARLLLRLHYPSEGYLVQEARGACFLGWQTR 428
Query: 543 PIHSISAWK 551
P+ S+SAW+
Sbjct: 429 PLLSVSAWQ 437
>DOA_DROME (P49762) Serine/threonine-protein kinase Doa (EC
2.7.1.37) (Protein darkener of apricot)
Length = 832
Score = 36.2 bits (82), Expect = 0.23
Identities = 22/76 (28%), Positives = 37/76 (47%), Gaps = 15/76 (19%)
Query: 18 EHEKEDHFSKEQNQQQQQQHDHFSFD-NLMFQDFY-------------FEPALEPKAAFQ 63
+H+++ H ++Q QQQQH H SF +M +++Y F+P PK
Sbjct: 289 QHQQQQHLQQQQQHPQQQQHQHSSFGVGMMSRNYYNMPKQPERKPLQTFDPYAYPKPNQM 348
Query: 64 EP-KEQKNRGVESSQF 78
+P K Q+ + +QF
Sbjct: 349 QPVKYQQQQQHPHTQF 364
>DRPL_HUMAN (P54259) Atrophin-1 (Dentatorubral-pallidoluysian
atrophy protein)
Length = 1185
Score = 35.4 bits (80), Expect = 0.39
Identities = 14/32 (43%), Positives = 20/32 (61%)
Query: 6 GLTNTSNPPPTTEHEKEDHFSKEQNQQQQQQH 37
G +T++PP +T H ++Q QQQQQQH
Sbjct: 467 GAQSTAHPPVSTHHHHHQQQQQQQQQQQQQQH 498
>DSH_DROME (P51140) Segment polarity protein dishevelled
Length = 623
Score = 33.9 bits (76), Expect = 1.1
Identities = 28/117 (23%), Positives = 43/117 (35%), Gaps = 10/117 (8%)
Query: 6 GLTNTSNPPPTTEHEKEDHFSKEQNQQQQQQH----------DHFSFDNLMFQDFYFEPA 55
GLTN +H+++ ++Q+QQQQQQ L Q P
Sbjct: 112 GLTNRKLQQQQQQHQQQQQQQQQQHQQQQQQQQQQVQPVQLAQQQQQQVLHHQKMMGNPL 171
Query: 56 LEPKAAFQEPKEQKNRGVESSQFFGFNKHLVGSNKQVPPSSLASLDLLKNYGSRFKR 112
L+P + + ++S+ FG L SS+ L + K R KR
Sbjct: 172 LQPPPLTYQSASVLSSDLDSTSLFGTESELTLDRDMTDYSSVQRLQVRKKPQRRKKR 228
>SR72_SCHPO (O59787) Signal recognition particle 72 kDa protein
homolog (SRP72)
Length = 561
Score = 33.1 bits (74), Expect = 1.9
Identities = 46/207 (22%), Positives = 95/207 (45%), Gaps = 16/207 (7%)
Query: 204 LQNFQGNSPHHGSAVQ----RVIFHFAQALQ-ERINKENGTTLKGSDERVKERHLIELLE 258
L+N NS H S + ++ F + QA E+ +KE+ LK S + + E + +
Sbjct: 170 LENSDSNSEDHKSQINLCRLQLFFAYLQAGDNEKASKES---LKISKDCLDETS--QAIF 224
Query: 259 VDTNITILCHQKMPFYQVMQFAGVQAILENVASGTKIHLININIGCGVMCTGLMQAL-SE 317
V+ I++ ++ + ++ L ++ + K I N+ M G +++ E
Sbjct: 225 VNNLISMSIDNPYISFRDLHGTNLEKALSSLLASQKKQFIR-NLALLDMAVGKQRSVRKE 283
Query: 318 RQENPVEILKITAVGVANKTKIEETGKRLMSFAESLNLPFSYNMIFVTDMMEIKVEQFDV 377
++ NP E + + + + +TK + K+L + E+L S N++ +M+ K+ +
Sbjct: 284 KKRNPEESIYFSTILLREETKSLISPKKLPGYLENLFKSDSDNIVVALLLMQHKISNGNF 343
Query: 378 EDDEAVAVYSPYFLRSLVSDSDSLENL 404
A+++Y LR+ + S SL L
Sbjct: 344 RG--ALSIYQK--LRTSLEASQSLSVL 366
>PUR2_PYRFU (Q8U3N5) Phosphoribosylamine--glycine ligase (EC
6.3.4.13) (GARS) (Glycinamide ribonucleotide synthetase)
(Phosphoribosylglycinamide synthetase)
Length = 439
Score = 33.1 bits (74), Expect = 1.9
Identities = 27/105 (25%), Positives = 55/105 (51%), Gaps = 15/105 (14%)
Query: 320 ENPVEILKITAVGVANKTKIEETGKRLMSFAESLNLPFSYNMIFVTDMMEIKVEQFDVED 379
ENP++ ++I N+ KI E G RL+ FA ++ +Y ++ + + V + ++E+
Sbjct: 342 ENPIKGVRIEV----NEDKIREEGARLI-FA---SIDENYTLLGSRALAVVGVSE-NLEE 392
Query: 380 DEAVA------VYSPYFLRSLVSDSDSLENLMKVLRKIKPNIMIN 418
E +A V P F R V +S+E +K+++ ++ + +N
Sbjct: 393 AEKIAQSAIRHVKGPIFYRKDVGTRESIEKRIKIMKSLRGDFDVN 437
>HUNB_DROSL (O46256) Hunchback protein (Fragments)
Length = 196
Score = 32.3 bits (72), Expect = 3.3
Identities = 14/34 (41%), Positives = 18/34 (52%)
Query: 12 NPPPTTEHEKEDHFSKEQNQQQQQQHDHFSFDNL 45
NPP T + E + ++Q QQQQQQ D L
Sbjct: 53 NPPSNTNLQLEQYLKQQQQQQQQQQQQQQPMDTL 86
>PK2_DICDI (P28178) Protein kinase 2 (EC 2.7.1.-)
Length = 479
Score = 32.0 bits (71), Expect = 4.3
Identities = 27/89 (30%), Positives = 39/89 (43%), Gaps = 5/89 (5%)
Query: 8 TNTSNPPPTTEHEKEDHFSKEQNQQQQQQHDHFSFDNLMFQDFYFEPALEPKAAFQEPKE 67
T+T P PT + D+ +++Q QQQQ S DN Q PA A Q P
Sbjct: 34 TSTPTPTPTPTQQNLDNSAQQQQQQQQTATAAVSLDNKEQQQQQNIPA----PATQTPIT 89
Query: 68 QKNR-GVESSQFFGFNKHLVGSNKQVPPS 95
Q +E SQ N ++ G++ + S
Sbjct: 90 QTGTPTIEESQKNTDNNNINGASNEASSS 118
>DGAL_CITFR (P23925) D-galactose-binding periplasmic protein
precursor (GBP) (D-galactose/ D-glucose binding protein)
(GGBP)
Length = 332
Score = 32.0 bits (71), Expect = 4.3
Identities = 16/45 (35%), Positives = 25/45 (55%)
Query: 501 VETRLSESAVYQANLVAKKFACGNSCTIDKNGKCLLIGWKGTPIH 545
V T ES + Q +L+AK +A + ++K+GK + KG P H
Sbjct: 131 VGTDSKESGIIQGDLIAKHWAANPNWDLNKDGKIQFVLLKGEPGH 175
>USPA_HAEIN (P44880) Universal stress protein A homolog
Length = 141
Score = 31.6 bits (70), Expect = 5.6
Identities = 27/123 (21%), Positives = 54/123 (42%), Gaps = 20/123 (16%)
Query: 294 KIHLININIGCGVMCTGLMQALSERQENPVEILKITAVGVANKTKIEETGKRLMSFAESL 353
K+ +I++++ + TGL+ ++ + ET K L+ AES+
Sbjct: 32 KLSIIHVDVNFSDLYTGLIDVNMSSMQDRIST---------------ETQKALLDLAESV 76
Query: 354 NLPFSYNMIFVTDMMEI---KVEQFDVEDDEAVAVYSPYFLRSLVSDSDSLENLMKVLRK 410
+ P S + D+ ++ +EQ+DV D V + F L+S + + N +K+
Sbjct: 77 DYPISEKLSGSGDLGQVLSDAIEQYDV--DLLVTGHHQDFWSKLMSSTRQVMNTIKIDML 134
Query: 411 IKP 413
+ P
Sbjct: 135 VVP 137
>KEN_DROME (O77459) Probable transcription factor Ken (Ken and
Barbie protein)
Length = 601
Score = 31.6 bits (70), Expect = 5.6
Identities = 13/33 (39%), Positives = 16/33 (48%)
Query: 7 LTNTSNPPPTTEHEKEDHFSKEQNQQQQQQHDH 39
+ N SN PP+ + S N QQQQ H H
Sbjct: 221 IVNLSNAPPSGTSGSNSNISTSSNHQQQQHHHH 253
>KCN3_HUMAN (Q9UGI6) Small conductance calcium-activated potassium
channel protein 3 (SK3) (SKCa3)
Length = 736
Score = 31.6 bits (70), Expect = 5.6
Identities = 26/94 (27%), Positives = 39/94 (40%), Gaps = 13/94 (13%)
Query: 14 PPTTEHEKEDHFSKEQNQQQQQQHDHFSFDNLMFQDFYFEPAL--EPKAAFQEPKEQKNR 71
PP + +++ ++Q QQQQQQ H Q P L AF+ P +
Sbjct: 63 PPQLQQQQQQQQQQQQQQQQQQQPPHPLSQLAQLQSQPVHPGLLHSSPTAFRAPPSSNST 122
Query: 72 GV------ESSQFFGFNKHLVGSNKQVPPSSLAS 99
+ + SQ N HL+G + PSS A+
Sbjct: 123 AILHPSSRQGSQ-LNLNDHLLGHS----PSSTAT 151
>HUNB_DROAD (O46232) Hunchback protein (Fragments)
Length = 192
Score = 31.6 bits (70), Expect = 5.6
Identities = 16/34 (47%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Query: 12 NPPPTTEHEKEDHFSKEQNQQQQQQHDHFSFDNL 45
NPP T + E + K Q QQQQQQH D L
Sbjct: 53 NPPSNTNLQLEQYL-KHQQQQQQQQHQQQPMDTL 85
>DX_DROME (Q23985) Deltex protein
Length = 738
Score = 31.6 bits (70), Expect = 5.6
Identities = 12/33 (36%), Positives = 18/33 (54%)
Query: 7 LTNTSNPPPTTEHEKEDHFSKEQNQQQQQQHDH 39
+T +P PT+ ++ + QQQQQQH H
Sbjct: 248 MTRQQHPLPTSHQVQQQQHQHQHQQQQQQQHHH 280
>REPE_ONYPE (P60470) Geminivirus-like replication protein
Length = 434
Score = 31.2 bits (69), Expect = 7.4
Identities = 25/106 (23%), Positives = 53/106 (49%), Gaps = 11/106 (10%)
Query: 25 FSKEQNQQQQQQHDHFSFDNLMFQDFYFEPALEPKAA--FQEPKEQKNRGVESSQFF--G 80
F+ +N ++++ DH +N+ +DF++ P +EP+ ++ + +N + F G
Sbjct: 83 FNIPKNIKEKKLIDH---ENIKIKDFFYSPHIEPRPGNPIKDTTDVRNYVKKDGDFIEEG 139
Query: 81 FNKHL--VGSNKQVPPSSLASLDLLKNYGSRFKRPRGQNILSKTKT 124
KH+ + +K P+ L S L NY + ++ Q ++ +KT
Sbjct: 140 TFKHVRYIKLSKNEKPTELES--LTNNYFLKLRKEYQQKEINISKT 183
>SRY_MOUSE (Q05738) Sex-determining region Y protein
(Testis-determining factor)
Length = 395
Score = 30.8 bits (68), Expect = 9.6
Identities = 14/53 (26%), Positives = 26/53 (48%)
Query: 19 HEKEDHFSKEQNQQQQQQHDHFSFDNLMFQDFYFEPALEPKAAFQEPKEQKNR 71
H+++ F Q QQQQQQ F + Q F+ + + F + ++Q+ +
Sbjct: 218 HQQQQQFHDHQQQQQQQQQQQFHDHHQQKQQFHDHHHHQQQQQFHDHQQQQQQ 270
>INVO_GALCR (P24710) Involucrin
Length = 384
Score = 30.8 bits (68), Expect = 9.6
Identities = 17/58 (29%), Positives = 27/58 (46%)
Query: 15 PTTEHEKEDHFSKEQNQQQQQQHDHFSFDNLMFQDFYFEPALEPKAAFQEPKEQKNRG 72
P E+E H K+Q QQ+ QQH ++ +D + E Q+ +EQ + G
Sbjct: 247 PQGPQEQELHLGKQQQQQESQQHQEQHEEHQKAEDLGQQHRQEKAQREQQLEEQLDEG 304
>DGAL_ECOLI (P02927) D-galactose-binding periplasmic protein
precursor (GBP) (D-galactose/ D-glucose binding protein)
(GGBP)
Length = 332
Score = 30.8 bits (68), Expect = 9.6
Identities = 15/45 (33%), Positives = 24/45 (53%)
Query: 501 VETRLSESAVYQANLVAKKFACGNSCTIDKNGKCLLIGWKGTPIH 545
V T ES + Q +L+AK +A ++K+G+ + KG P H
Sbjct: 131 VGTDSKESGIIQGDLIAKHWAANQGWDLNKDGQIQFVLLKGEPGH 175
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.134 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,040,503
Number of Sequences: 164201
Number of extensions: 2485518
Number of successful extensions: 9816
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 9727
Number of HSP's gapped (non-prelim): 69
length of query: 553
length of database: 59,974,054
effective HSP length: 115
effective length of query: 438
effective length of database: 41,090,939
effective search space: 17997831282
effective search space used: 17997831282
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0239.10