BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0234.13
(1451 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergeni... 89 7e-17
PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1, m... 57 5e-07
PIF1_YEAST (P07271) DNA repair and recombination protein PIF1, m... 56 8e-07
EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 38 0.18
PRIA_BORBU (Q45032) Primosomal protein N' (Replication factor Y) 34 2.6
GSA_THEVO (Q97B25) Probable glutamate-1-semialdehyde 2,1-aminomu... 34 2.6
Y075_METJA (Q60388) Hypothetical ATP-binding protein MJ0075 34 3.3
HELI_EHV1B (P28934) Probable helicase 34 3.3
EX5A_HAEIN (P45158) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 34 3.3
CLPB_MYCLE (Q9CB26) Chaperone clpB 34 3.3
CLPB_FUSNN (Q8RHQ8) Chaperone clpB 33 4.4
KINH_SYNRA (O43093) Kinesin heavy chain (Synkin) 33 5.7
PYRB_TREDE (Q04595) PYRBI protein [Includes: Aspartate carbamoyl... 33 7.4
KFMS_RAT (Q00495) Macrophage colony stimulating factor I recepto... 33 7.4
RPIA_CHLTR (O84215) Ribose-5-phosphate isomerase A (EC 5.3.1.6) ... 32 9.7
HELI_HCMVA (P16736) Probable helicase 32 9.7
>YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergenic
region
Length = 723
Score = 89.4 bits (220), Expect = 7e-17
Identities = 156/658 (23%), Positives = 279/658 (41%), Gaps = 103/658 (15%)
Query: 825 FQEACSALGLLEDDKEFIDGLIDCAELSG-GMSVRKLFMVLLLSN--SIVTPGKVF---- 877
F ++ + G + D EF L+D L+ S R L M L S T K +
Sbjct: 89 FSQSQGSFGDDDPDAEF-KKLVDVPRLNSYKKSSRSLSMTSSLHKTASASTTQKTYHFDE 147
Query: 878 EETWRLLADGILYKRRKLLCNPALRLDDQTLKTLCLVELEKMLVNNGKTLKDFPGIPYPI 937
+ET R + + R+L + ++D ++K L + K +F G+ +
Sbjct: 148 DETLREVTS-VKSNSRQLSFTSTINIEDSSMK---LSTDSERPAKRSKPSMEFQGLKLTV 203
Query: 938 SDEVPQFENVMLFNELRFDIDDMSVKHNDHLMKLNNGQRKVYDEVIDAVNKSDGGFYFVY 997
++ L + ++D M+ + + L Q +V + + V K FY
Sbjct: 204 PKKIKP-----LLRKTVSNMDSMNHRSASSPVVLTMEQERVVNLI---VKKRTNVFYT-- 253
Query: 998 GSGGTGKTFLWKTLSYRLRS--ERKIVLNVASSGITSLLLPGGRTAHSLFSIPLVLNEDS 1055
GS GTGK+ + +T+ +L S ++ + AS+G+ ++ + GG T H I +
Sbjct: 254 GSAGTGKSVILQTIIRQLSSLYGKESIAITASTGLAAVTI-GGSTLHKWSGIGIGNKTID 312
Query: 1056 CCNIRLGSNKAELL--KHTSLIIWDEAPMVNRWAFEAVDRTLRDIMEVGDVYGGGKPFGG 1113
++ S K L ++T ++I DE MV+ + +++ R I + D PFGG
Sbjct: 313 QLVKKIQSQKDLLAAWRYTKVLIIDEISMVDGNLLDKLEQIARRIRKNDD------PFGG 366
Query: 1114 KVVVLGGDFRQTLPIIPKASREEIVMATINSSRLWKFC--------KVLKLTENMCLHGN 1165
+VL GDF Q P+ K E V+ S +WK C KV + +N +
Sbjct: 367 IQLVLTGDFFQLPPVAKK--DEHNVVKFCFESEMWKRCIQKTILLTKVFRQQDNKLIDIL 424
Query: 1166 DSLHDCEKLVEFSKWILDIGDGNLGDYNDGECDLDIPHDLMVPFKDDAVSSI-------- 1217
+++ E V+ +K I ++ DY DG P +L ++ +S++
Sbjct: 425 NAIRYGELTVDIAKTIRNLNRDI--DYADGIA----PTELYATRREVELSNVKKLQSLPG 478
Query: 1218 ------VYSTYPDIQRKFFEEEYFIDKAI-LAPTLDIVDSVNQFVLSIVP---GKEKVYL 1267
P+ + + ++K + L ++ N+ + +V GK ++
Sbjct: 479 DLYEFKAVDNAPERYQAILDSSLMVEKVVALKEDAQVMMLKNKPDVELVNGSLGKVLFFV 538
Query: 1268 SSDSVVKVDEDVAIDANWITIEFLNGIKGSGLPDHRLCLK-IGTPIMLMRNIDVSAGLCN 1326
+ VVK+ E I + + D RL + IG P +L + + L N
Sbjct: 539 TESLVVKMKEIYKI------------VDDEVVMDMRLVSRVIGNP-LLKESKEFRQDL-N 584
Query: 1327 GTRLIVLDLRPNLI-YGKVLNGNK---------AGTKTYIPRMTI-------VPSDSGLH 1369
L L+ LI Y ++ +K G YI + + +P + +
Sbjct: 585 ARPLARLERLKILINYAVKISPHKEKFPYVRWTVGKNKYIHELMVPERFPIDIPRE---N 641
Query: 1370 VKVQRRQFPVCVCFAMTINKSQGQTLSRVGVFLPRPVFSHGQLYVALSRVKSRDGLKI 1427
V ++R Q P+ +C+A++I+K+QGQT+ R+ V L R +F GQ+YVALSR + D L++
Sbjct: 642 VGLERTQIPLMLCWALSIHKAQGQTIQRLKVDL-RRIFEAGQVYVALSRAVTMDTLQV 698
>PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1,
mitochondrial precursor
Length = 805
Score = 56.6 bits (135), Expect = 5e-07
Identities = 51/173 (29%), Positives = 84/173 (48%), Gaps = 23/173 (13%)
Query: 971 LNNGQRKVYDEVIDAVNKSDGGFYFVYGSGGTGKTFLWKTL------SYRLRSERKIVLN 1024
L++ Q+++ D V++ + F GS GTGK+ L + + YR +S+R V
Sbjct: 310 LSDEQKRILDMVVEQQHS-----IFFTGSAGTGKSVLLRKIIEVLKSKYRKQSDRVAV-- 362
Query: 1025 VASSGITSLLLPGGRTAHSLFSIPLVLNEDSCCNIRLGSNKAELLK--HTSLIIWDEAPM 1082
AS+G+ + + GG T HS + L ++ NK + + T ++I DE M
Sbjct: 363 TASTGLAACNI-GGVTLHSFAGVGLARESVDLLVSKIKKNKKCVNRWLRTRVLIIDEVSM 421
Query: 1083 VNRWAFEAVDRTLRDIMEVGDVYGGGKPFGGKVVVLGGDFRQTLPIIPKASRE 1135
V+ + ++ R I + KPFGG +VL GDF Q LP +P+ +E
Sbjct: 422 VDAELMDKLEEVARVIRK------DSKPFGGIQLVLTGDFFQ-LPPVPENGKE 467
Score = 56.2 bits (134), Expect = 6e-07
Identities = 29/58 (50%), Positives = 42/58 (72%), Gaps = 1/58 (1%)
Query: 1370 VKVQRRQFPVCVCFAMTINKSQGQTLSRVGVFLPRPVFSHGQLYVALSRVKSRDGLKI 1427
V+ R Q P+ + +A++I+K+QGQTL RV V L R VF GQ YVALSR +++GL++
Sbjct: 704 VQASRSQIPLILAYAISIHKAQGQTLDRVKVDLGR-VFEKGQAYVALSRATTQEGLQV 760
>PIF1_YEAST (P07271) DNA repair and recombination protein PIF1,
mitochondrial precursor
Length = 857
Score = 55.8 bits (133), Expect = 8e-07
Identities = 93/387 (24%), Positives = 157/387 (40%), Gaps = 70/387 (18%)
Query: 950 FNELRFDIDDMSVK-HNDHLMKLNNGQRKVYDEVIDAVNKSDGGFYFVYGSGGTGKTFLW 1008
F EL+ D DD ++ HN +K+ K + +I FY GS GTGK+ L
Sbjct: 211 FTELQNDQDDSNLNPHNGVKVKIPICLSKEQESIIKLAENGHNIFYT--GSAGTGKSILL 268
Query: 1009 KTLSYRLRS--ERKIVLNVASSGITSLLLPGGRTAHSLFSIPLVLNEDSCCNIRLGSNKA 1066
+ + L+ R+ V AS+G+ + + GG T HS I + D ++G
Sbjct: 269 REMIKVLKGIYGRENVAVTASTGLAACNI-GGITIHSFAGILGKGDADKLYK-KVGRRSR 326
Query: 1067 ELLK---HTSLIIWDEAPMVNRWAFEAVDRTLRDIMEVGDVYGGGKPFGGKVVVLGGDFR 1123
+ L+ + ++ DE M++ + +D R I + +PFGG ++ GDF
Sbjct: 327 KHLRRWENIGALVVDEISMLDAELLDKLDFIARKIRK------NHQPFGGIQLIFCGDFF 380
Query: 1124 QTLPIIPKASREEIVMATINSSRLWK-FCKVLKLTENMCLHGNDSLHDCEKLVEFSKWIL 1182
Q LP + K A S+ WK K+ + + + D V+F + +
Sbjct: 381 Q-LPPVSKDPNRPTKFAF--ESKAWKEGVKMTIMLQKVFRQRGD--------VKFIEMLN 429
Query: 1183 DIGDGNLGDYNDGECDLDIPHDLMVPFKDD-AVSSIVYSTYPDIQRKFFEEEYFIDKAIL 1241
+ GN+ D + E L P DD + + +YST +++R
Sbjct: 430 RMRLGNIDDETEREF-----KKLSRPLPDDEIIPAELYSTRMEVER-------------- 470
Query: 1242 APTLDIVDSVNQFVLSIVPGKEKVYLSSDSVVKVDEDVAIDANWITIEFLNGIKGSGLPD 1301
N LS +PG+ ++ + D DE E + + L
Sbjct: 471 ---------ANNSRLSKLPGQVHIFNAIDGGALEDE-----------ELKERLLQNFLAP 510
Query: 1302 HRLCLKIGTPIMLMRNIDVSAGLCNGT 1328
L LK+G +M+++N+D A L NG+
Sbjct: 511 KELHLKVGAQVMMVKNLD--ATLVNGS 535
Score = 55.8 bits (133), Expect = 8e-07
Identities = 32/64 (50%), Positives = 45/64 (70%), Gaps = 2/64 (3%)
Query: 1372 VQRRQFPVCVCFAMTINKSQGQTLSRVGVFLPRPVFSHGQLYVALSRVKSRDGLKIY-VD 1430
V R Q P+ + ++++I+KSQGQTL +V V L R VF GQ YVALSR SR+GL++ D
Sbjct: 689 VSRVQLPLMLAWSLSIHKSQGQTLPKVKVDL-RRVFEKGQAYVALSRAVSREGLQVLNFD 747
Query: 1431 QTEV 1434
+T +
Sbjct: 748 RTRI 751
>EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)
(Exodeoxyribonuclease V 67 kDa polypeptide)
Length = 608
Score = 38.1 bits (87), Expect = 0.18
Identities = 37/127 (29%), Positives = 54/127 (42%), Gaps = 26/127 (20%)
Query: 1309 GTPIMLMRNIDVSAGLCNGTRLIVLDLRPNLIYGKVLNGNKAGTKTYIPRMTIVPSDSGL 1368
G P+M+ RN D + GL NG I LD GT+ + G
Sbjct: 479 GRPVMIARN-DSALGLFNGDIGIALD-------------RGQGTRVWFAM------PDGN 518
Query: 1369 HVKVQRRQFPVC-VCFAMTINKSQGQTLSRVGVFLP---RPVFSHGQLYVALSRVKSRDG 1424
VQ + P +AMT++KSQG + LP PV + +Y A++R + R
Sbjct: 519 IKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRR-- 576
Query: 1425 LKIYVDQ 1431
L +Y D+
Sbjct: 577 LSLYADE 583
>PRIA_BORBU (Q45032) Primosomal protein N' (Replication factor Y)
Length = 660
Score = 34.3 bits (77), Expect = 2.6
Identities = 19/63 (30%), Positives = 38/63 (60%), Gaps = 6/63 (9%)
Query: 963 KHNDH--LMKLNNGQRKVYDEVIDAVNKSDGGFYFVYGSGGTGKTFLW-KTLSYRLRSER 1019
+H DH ++LNN Q+ +Y E+I + + ++++G G+GKT ++ K Y L E+
Sbjct: 124 EHPDHKKCLELNNEQQNIYKEIIGSEKTN---VFYLFGIPGSGKTEIFIKLCEYYLALEQ 180
Query: 1020 KIV 1022
+++
Sbjct: 181 QVL 183
>GSA_THEVO (Q97B25) Probable glutamate-1-semialdehyde 2,1-aminomutase
(EC 5.4.3.8) (GSA) (Glutamate-1-semialdehyde
aminotransferase) (GSA-AT)
Length = 420
Score = 34.3 bits (77), Expect = 2.6
Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 3/46 (6%)
Query: 1070 KHTSLIIWDEAPMVNRWAFEAVDRTLR---DIMEVGDVYGGGKPFG 1112
KH SL+I+DE R+AF +R DI +G + GGG P G
Sbjct: 225 KHGSLLIFDEVITGYRFAFSPFQDIMRIDPDITTMGKIIGGGLPIG 270
>Y075_METJA (Q60388) Hypothetical ATP-binding protein MJ0075
Length = 365
Score = 33.9 bits (76), Expect = 3.3
Identities = 15/53 (28%), Positives = 33/53 (61%), Gaps = 1/53 (1%)
Query: 968 LMKLNNGQRKVYDEVIDAVNKSDGGFYFVYGSGGTGKTFLWKTLSYRLRSERK 1020
+MK + +R++ +E++ ++++ YF+YG +GKT L + RL+ ++K
Sbjct: 2 IMKFFDREREI-NEILGILDETPDNIYFIYGPINSGKTTLMMEIINRLKDDKK 53
>HELI_EHV1B (P28934) Probable helicase
Length = 881
Score = 33.9 bits (76), Expect = 3.3
Identities = 18/44 (40%), Positives = 26/44 (58%)
Query: 1384 AMTINKSQGQTLSRVGVFLPRPVFSHGQLYVALSRVKSRDGLKI 1427
AMTI +SQG +L +V + PR +YVA+SR S L++
Sbjct: 806 AMTIARSQGLSLDKVAICFPRNNLRINSVYVAMSRTVSSRFLRM 849
>EX5A_HAEIN (P45158) Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)
Length = 640
Score = 33.9 bits (76), Expect = 3.3
Identities = 52/218 (23%), Positives = 80/218 (35%), Gaps = 64/218 (29%)
Query: 1249 DSVNQFVLSIVPG------------KEKVYLSSDSVVKVDEDVAIDANWITIEFLNGIKG 1296
D+VNQ V S V K+K+ ++ + + AI + ++ FL ++
Sbjct: 427 DAVNQVVKSAVENYRTFLKMLDDLRKQKIDPNAKNEQGISYAEAIQVQFNSVRFLTALRN 486
Query: 1297 SGL------PDHRLCLK--------------IGTPIMLMRNIDVSAGLCNGTRLIVLDLR 1336
+ L + L L+ IG PIM+ N D + L NG D+
Sbjct: 487 NNLGVENLNKEIALALREQKLLWFRNEQDWYIGKPIMITEN-DHNVRLYNG------DIG 539
Query: 1337 PNLIYGKVLNGNKAGTKTYIPRMTIVPSDSGLHVKVQRRQFPVCVCFAMTINKSQGQTLS 1396
L GKV GN+ IP F MTI+KSQG
Sbjct: 540 LCLANGKVWFGNREVLTNRIPAHE--------------------PAFMMTIHKSQGSEFK 579
Query: 1397 RVGVFLP---RPVFSHGQLYVALSRVKSRDGLKIYVDQ 1431
+ LP PV S ++ ++R K L ++ D+
Sbjct: 580 HTVMVLPTEVNPVLSRELVFTGVTRAKKE--LTVFADE 615
>CLPB_MYCLE (Q9CB26) Chaperone clpB
Length = 848
Score = 33.9 bits (76), Expect = 3.3
Identities = 48/198 (24%), Positives = 79/198 (39%), Gaps = 37/198 (18%)
Query: 962 VKHNDHLMKLNNGQRKVYDEVIDAVNKSDGGF---------YFVYGSGGTGKTFLWKTLS 1012
++ D L K GQ+K V DAV +S G + G G GKT L K L+
Sbjct: 562 LRMEDELGKRVVGQKKAVHAVSDAVRRSRAGVADPNRPTGSFLFLGPTGVGKTELAKALA 621
Query: 1013 YRLRSERKIVLNVASSGITSLLLPGGRTAHSLFSIPLVLNEDSCCNIRLGSNKAELLKHT 1072
L +++ ++ V S A + + P + D G E ++
Sbjct: 622 DFLFDDQRAMVRVDMSEYGE----KHSVARLVGAPPGYIGHDQ------GGQLTEAVRRR 671
Query: 1073 --SLIIWDEAPMVNRWAFEAVDRTLRDIMEVGDVYGGGKPFGGKVVVLGGDFRQTLPIIP 1130
+++++DE + F+ L +++ G + G G+ V DFR T+PI+
Sbjct: 672 PYTVVLFDEVEKAHSDVFD----VLLQVLDEGRLTDG----QGRTV----DFRNTIPILT 719
Query: 1131 ----KASREEIVMATINS 1144
EE VMA + S
Sbjct: 720 SNLGSGGSEEQVMAAVRS 737
>CLPB_FUSNN (Q8RHQ8) Chaperone clpB
Length = 857
Score = 33.5 bits (75), Expect = 4.4
Identities = 23/73 (31%), Positives = 33/73 (44%), Gaps = 11/73 (15%)
Query: 966 DHLMKLNNGQRKVYDEVIDAV----------NKSDGGFYFVYGSGGTGKTFLWKTLSYRL 1015
DH+ + GQ + V D + N+ G F F+ G G GKT+L KTL+Y L
Sbjct: 565 DHIKERVKGQDEAIKSVADTMLRSVAGLKDPNRPMGSFIFL-GPTGVGKTYLAKTLAYNL 623
Query: 1016 RSERKIVLNVASS 1028
V+ + S
Sbjct: 624 FDSEDNVVRIDMS 636
>KINH_SYNRA (O43093) Kinesin heavy chain (Synkin)
Length = 935
Score = 33.1 bits (74), Expect = 5.7
Identities = 16/45 (35%), Positives = 23/45 (50%), Gaps = 4/45 (8%)
Query: 966 DHLMKLNNGQRKVYD----EVIDAVNKSDGGFYFVYGSGGTGKTF 1006
D + +N Q+ V+D ++D V G F YG G+GKTF
Sbjct: 51 DKVFGMNTAQKDVFDYSIKTIVDDVTAGYNGTVFAYGQTGSGKTF 95
>PYRB_TREDE (Q04595) PYRBI protein [Includes: Aspartate
carbamoyltransferase (EC 2.1.3.2) (Aspartate
transcarbamylase) (ATCase); Aspartate
carbamoyltransferase regulatory region]
Length = 535
Score = 32.7 bits (73), Expect = 7.4
Identities = 12/24 (50%), Positives = 16/24 (66%)
Query: 18 DRKYECQYCGALHWYAEGVRKKKN 41
D +Y CQYCG +H + E +KKN
Sbjct: 512 DNRYACQYCGTIHTFKEIWGEKKN 535
>KFMS_RAT (Q00495) Macrophage colony stimulating factor I receptor
precursor (CSF-1-R) (EC 2.7.1.112) (Fms proto-oncogene)
(c-fms)
Length = 978
Score = 32.7 bits (73), Expect = 7.4
Identities = 16/36 (44%), Positives = 23/36 (63%)
Query: 599 GVFVERRDVQLDNGYVVPYNAKLLMKYQAHINIVYC 634
G F RD+ D+ YVV NA+L +K+ A +I+YC
Sbjct: 793 GDFGLARDIMNDSNYVVKGNARLPVKWMAPESILYC 828
>RPIA_CHLTR (O84215) Ribose-5-phosphate isomerase A (EC 5.3.1.6)
(Phosphoriboisomerase A) (PRI)
Length = 242
Score = 32.3 bits (72), Expect = 9.7
Identities = 20/52 (38%), Positives = 30/52 (57%), Gaps = 1/52 (1%)
Query: 983 IDAVNKSDGGFYFVYGSGGTGKTFLWKTLSYRLRSERKIVLNVASSGITSLL 1034
++AV + G GSG T + F+ L R+R+ER ++ VASS I+ LL
Sbjct: 23 LEAVALVEPGMCVGLGSGSTAREFIL-ALGDRVRTERLVITAVASSRISQLL 73
>HELI_HCMVA (P16736) Probable helicase
Length = 956
Score = 32.3 bits (72), Expect = 9.7
Identities = 23/59 (38%), Positives = 33/59 (54%), Gaps = 2/59 (3%)
Query: 1384 AMTINKSQGQTLSRVGV-FLPRPV-FSHGQLYVALSRVKSRDGLKIYVDQTEVPPLEHT 1440
AMTI KSQG +L +V V F P +YVA+SRV + L + V+ +P ++T
Sbjct: 879 AMTIAKSQGLSLEKVAVDFGDHPKNLKMSHIYVAMSRVTDPEHLMMNVNPLRLPYEKNT 937
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.141 0.433
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 180,946,178
Number of Sequences: 164201
Number of extensions: 8279996
Number of successful extensions: 17848
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 17825
Number of HSP's gapped (non-prelim): 23
length of query: 1451
length of database: 59,974,054
effective HSP length: 123
effective length of query: 1328
effective length of database: 39,777,331
effective search space: 52824295568
effective search space used: 52824295568
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 72 (32.3 bits)
Lotus: description of TM0234.13