BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0233.16
(1520 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PIF1_YEAST (P07271) DNA repair and recombination protein PIF1, m... 55 2e-06
PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1, m... 55 2e-06
YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergeni... 54 3e-06
EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 37 0.41
SODN_BACCR (Q814I6) Superoxide dismutase [Mn] 2 (EC 1.15.1.1) 35 1.6
HELI_EHV1B (P28934) Probable helicase 35 1.6
PRIA_BORBU (Q45032) Primosomal protein N' (Replication factor Y) 35 2.1
SODN_BACAN (Q81JK8) Superoxide dismutase [Mn] 2 (EC 1.15.1.1) 34 3.5
YF19_METJA (Q58914) Hypothetical protein MJ1519 33 7.8
HELI_VZVD (P09303) Probable helicase 33 7.8
>PIF1_YEAST (P07271) DNA repair and recombination protein PIF1,
mitochondrial precursor
Length = 857
Score = 54.7 bits (130), Expect = 2e-06
Identities = 32/80 (40%), Positives = 52/80 (65%), Gaps = 3/80 (3%)
Query: 1432 DESMPVKFQRRQFPLIASFAMTINKSQGQSLSQVGVYLPKPVFSHGQLYVAISRVKSRAG 1491
DE+ R Q PL+ +++++I+KSQGQ+L +V V L + VF GQ YVA+SR SR G
Sbjct: 682 DENEKPLVSRVQLPLMLAWSLSIHKSQGQTLPKVKVDL-RRVFEKGQAYVALSRAVSREG 740
Query: 1492 LKILICNEDTSQLDVTKNIV 1511
L++L N D +++ + ++
Sbjct: 741 LQVL--NFDRTRIKAHQKVI 758
Score = 44.3 bits (103), Expect = 0.003
Identities = 89/378 (23%), Positives = 154/378 (40%), Gaps = 75/378 (19%)
Query: 1034 CFSKLNDGQLKIYQEIITAINANNGGFFFVYGYGGTGKTFLWKTLTYKLRS--QKQIILN 1091
C SK + +K+ A NG F G GTGK+ L + + L+ ++ +
Sbjct: 236 CLSKEQESIIKL---------AENGHNIFYTGSAGTGKSILLREMIKVLKGIYGRENVAV 286
Query: 1092 VASSGIASLLLPGGRTAHSLFSIPLCLNEDSCCGIPQGSPKAKLLQ----LASLIIWDEA 1147
AS+G+A+ + GG T HS I L + G K L+ + +L++ DE
Sbjct: 287 TASTGLAACNI-GGITIHSFAGI-LGKGDADKLYKKVGRRSRKHLRRWENIGALVV-DEI 343
Query: 1148 PMVSRYAFEALDRTLRDIMRFKIHNSSDKPFGGKVVVLGGDFRQILPVIPKGRRAEIVMS 1207
M+ + LD R I + + +PFGG ++ GDF Q+ PV R
Sbjct: 344 SMLDAELLDKLDFIARKIRK------NHQPFGGIQLIFCGDFFQLPPVSKDPNRP---TK 394
Query: 1208 TINSSRLWRFCKVLNLTQNMRLTTSTCDGDNEEVKHFAKWVLDMGDGKLGDYNDGESNVQ 1267
S+ W+ + +++T + + K++ + +LG+ +D E+ +
Sbjct: 395 FAFESKAWK--------EGVKMTIMLQKVFRQ--RGDVKFIEMLNRMRLGNIDD-ETERE 443
Query: 1268 IPKDLLIYQSSNPIADIVHLMYPDIVQNVGCVKYYSDKAILAPTLDAVDSINQYALSLFP 1327
K S P+ D +I+ A L T V+ N LS P
Sbjct: 444 FKK------LSRPLPD------DEII-----------PAELYSTRMEVERANNSRLSKLP 480
Query: 1328 GNEKTYLSSDSVWRVDEDVGIEADWLTTEFLNGLKCSGMPDHKLVLKEGAPIMLMRNLDI 1387
G + + D DE++ + L FL +L LK GA +M+++NLD
Sbjct: 481 GQVHIFNAIDGGALEDEEL---KERLLQNFL--------APKELHLKVGAQVMMVKNLDA 529
Query: 1388 STGLCNGT-RLIVDYLGP 1404
+ L NG+ +++++ P
Sbjct: 530 T--LVNGSLGKVIEFMDP 545
>PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1,
mitochondrial precursor
Length = 805
Score = 54.7 bits (130), Expect = 2e-06
Identities = 92/356 (25%), Positives = 140/356 (38%), Gaps = 69/356 (19%)
Query: 1038 LNDGQLKIYQEIITAINANNGGFFFVYGYGGTGKTFLWK----TLTYKLRSQKQIILNVA 1093
L+D Q +I ++ ++ F G GTGK+ L + L K R Q + A
Sbjct: 310 LSDEQKRILDMVVEQQHS-----IFFTGSAGTGKSVLLRKIIEVLKSKYRKQSDRVAVTA 364
Query: 1094 SSGIASLLLPGGRTAHSLFSIPLCLNE-DSCCGIPQGSPKA--KLLQLASLIIWDEAPMV 1150
S+G+A+ + GG T HS + L D + + K + L+ LII DE MV
Sbjct: 365 STGLAACNI-GGVTLHSFAGVGLARESVDLLVSKIKKNKKCVNRWLRTRVLII-DEVSMV 422
Query: 1151 SRYAFEALDRTLRDIMRFKIHNSSDKPFGGKVVVLGGDFRQILPVIPKGRRAEIVMSTIN 1210
+ L+ R I + KPFGG +VL GDF Q+ PV G+ ++
Sbjct: 423 DAELMDKLEEVARVIRK------DSKPFGGIQLVLTGDFFQLPPVPENGKESKFCF---- 472
Query: 1211 SSRLWRFCKVLNLTQNMRLTTSTCDGDNEEVKHFAKWVLDMGDGKLGDYNDGESNVQIPK 1270
S+ W+ L+ T + LT D E F K + ++ GKL D +
Sbjct: 473 ESQTWK--SALDFT--IGLTHVFRQKDEE----FVKMLNELRLGKLSD-----------E 513
Query: 1271 DLLIYQSSNPIADIVHLMYPDIVQNVGCVKYYSDKAILAPTLDAVDSINQYALSLFPGNE 1330
+ ++ N + + P L PT V+ N + N
Sbjct: 514 SVRKFKVLNRTIEYEDGLLP---------------TELFPTRYEVERSNDMRMQQINQNP 558
Query: 1331 KTYLSSDSVWRVDEDVGIEADWLTTEFLNGLKCSGMPDHKLVLKEGAPIMLMRNLD 1386
T+ + DS G D EF + L M LVLK A +ML++N+D
Sbjct: 559 VTFTAIDS--------GTVRD---KEFRDRLLQGCMAPATLVLKVNAQVMLIKNID 603
Score = 53.5 bits (127), Expect = 4e-06
Identities = 28/59 (47%), Positives = 43/59 (72%), Gaps = 1/59 (1%)
Query: 1437 VKFQRRQFPLIASFAMTINKSQGQSLSQVGVYLPKPVFSHGQLYVAISRVKSRAGLKIL 1495
V+ R Q PLI ++A++I+K+QGQ+L +V V L + VF GQ YVA+SR ++ GL++L
Sbjct: 704 VQASRSQIPLILAYAISIHKAQGQTLDRVKVDLGR-VFEKGQAYVALSRATTQEGLQVL 761
>YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergenic
region
Length = 723
Score = 54.3 bits (129), Expect = 3e-06
Identities = 56/193 (29%), Positives = 90/193 (46%), Gaps = 17/193 (8%)
Query: 1043 LKIYQEIITAINANNGGFFFVYGYGGTGKTFLWKTLTYKLRSQ--KQIILNVASSGIASL 1100
L + QE + + F G GTGK+ + +T+ +L S K+ I AS+G+A++
Sbjct: 232 LTMEQERVVNLIVKKRTNVFYTGSAGTGKSVILQTIIRQLSSLYGKESIAITASTGLAAV 291
Query: 1101 LLPGGRTAHSLFSIPLCLNEDSCCGIPQGSPKAKLL---QLASLIIWDEAPMVSRYAFEA 1157
+ GG T H I + N+ + + + LL + ++I DE MV +
Sbjct: 292 TI-GGSTLHKWSGIGIG-NKTIDQLVKKIQSQKDLLAAWRYTKVLIIDEISMVDGNLLDK 349
Query: 1158 LDRTLRDIMRFKIHNSSDKPFGGKVVVLGGDFRQILPVIPKGRRAEIVMSTINSSRLWRF 1217
L++ R I + +D PFGG +VL GDF Q+ PV K V+ S +W+
Sbjct: 350 LEQIARRIRK------NDDPFGGIQLVLTGDFFQLPPVAKKDEHN--VVKFCFESEMWKR 401
Query: 1218 C--KVLNLTQNMR 1228
C K + LT+ R
Sbjct: 402 CIQKTILLTKVFR 414
Score = 45.1 bits (105), Expect = 0.002
Identities = 23/59 (38%), Positives = 41/59 (68%), Gaps = 1/59 (1%)
Query: 1437 VKFQRRQFPLIASFAMTINKSQGQSLSQVGVYLPKPVFSHGQLYVAISRVKSRAGLKIL 1495
V +R Q PL+ +A++I+K+QGQ++ ++ V L + +F GQ+YVA+SR + L++L
Sbjct: 642 VGLERTQIPLMLCWALSIHKAQGQTIQRLKVDL-RRIFEAGQVYVALSRAVTMDTLQVL 699
>EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)
(Exodeoxyribonuclease V 67 kDa polypeptide)
Length = 608
Score = 37.0 bits (84), Expect = 0.41
Identities = 36/121 (29%), Positives = 53/121 (43%), Gaps = 22/121 (18%)
Query: 1375 EGAPIMLMRNLDISTGLCNGTRLIVDYLGPNVIGAIVLSGTHIGKVVYISRMNLMPSDES 1434
EG P+M+ RN D + GL NG I G + +I K V SR+ P E+
Sbjct: 478 EGRPVMIARN-DSALGLFNGDIGIALDRGQGTRVWFAMPDGNI-KSVQPSRL---PEHET 532
Query: 1435 MPVKFQRRQFPLIASFAMTINKSQGQSLSQVGVYLPK---PVFSHGQLYVAISRVKSRAG 1491
++AMT++KSQG + LP PV + +Y A++R + R
Sbjct: 533 --------------TWAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRRLS 578
Query: 1492 L 1492
L
Sbjct: 579 L 579
>SODN_BACCR (Q814I6) Superoxide dismutase [Mn] 2 (EC 1.15.1.1)
Length = 208
Score = 35.0 bits (79), Expect = 1.6
Identities = 18/64 (28%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Query: 1001 PHVEANTLTEYG---NVLLLNELNFDVVEMSNLHDECFSKLNDGQLKIYQEIITAINANN 1057
PH+++NTL+ + + +N LN + + LH++ +L + +EI+TA+ N
Sbjct: 18 PHIDSNTLSIHHGKHHATYVNNLNATLENYTELHNKSLEELLCNLDTLPKEIVTAVRNNG 77
Query: 1058 GGFF 1061
GG +
Sbjct: 78 GGHY 81
>HELI_EHV1B (P28934) Probable helicase
Length = 881
Score = 35.0 bits (79), Expect = 1.6
Identities = 19/51 (37%), Positives = 29/51 (56%)
Query: 1444 FPLIASFAMTINKSQGQSLSQVGVYLPKPVFSHGQLYVAISRVKSRAGLKI 1494
+ L + AMTI +SQG SL +V + P+ +YVA+SR S L++
Sbjct: 799 YGLSSKLAMTIARSQGLSLDKVAICFPRNNLRINSVYVAMSRTVSSRFLRM 849
>PRIA_BORBU (Q45032) Primosomal protein N' (Replication factor Y)
Length = 660
Score = 34.7 bits (78), Expect = 2.1
Identities = 21/60 (35%), Positives = 36/60 (60%), Gaps = 5/60 (8%)
Query: 1031 HDECFSKLNDGQLKIYQEIITAINANNGGFFFVYGYGGTGKTFLW-KTLTYKLRSQKQII 1089
H +C +LN+ Q IY+EII + N F+++G G+GKT ++ K Y L ++Q++
Sbjct: 128 HKKCL-ELNNEQQNIYKEIIGSEKTN---VFYLFGIPGSGKTEIFIKLCEYYLALEQQVL 183
>SODN_BACAN (Q81JK8) Superoxide dismutase [Mn] 2 (EC 1.15.1.1)
Length = 208
Score = 33.9 bits (76), Expect = 3.5
Identities = 18/64 (28%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Query: 1001 PHVEANTLTEYG---NVLLLNELNFDVVEMSNLHDECFSKLNDGQLKIYQEIITAINANN 1057
P++++NTL+ + + +N LN + S LH++ +L + +EI+TA+ N
Sbjct: 18 PYIDSNTLSIHHGKHHATYVNNLNAALENYSELHNKSLEELLCNLETLPKEIVTAVRNNG 77
Query: 1058 GGFF 1061
GG +
Sbjct: 78 GGHY 81
>YF19_METJA (Q58914) Hypothetical protein MJ1519
Length = 1175
Score = 32.7 bits (73), Expect = 7.8
Identities = 18/44 (40%), Positives = 26/44 (58%), Gaps = 3/44 (6%)
Query: 1449 SFAMTINKSQGQSLSQVGVYLPKPV---FSHGQLYVAISRVKSR 1489
++A+TI+KSQG V + +PK + S LY AI+R K R
Sbjct: 960 AYAITIHKSQGSGFENVILIIPKGLNKFVSKEMLYTAITRAKKR 1003
>HELI_VZVD (P09303) Probable helicase
Length = 881
Score = 32.7 bits (73), Expect = 7.8
Identities = 19/51 (37%), Positives = 27/51 (52%)
Query: 1444 FPLIASFAMTINKSQGQSLSQVGVYLPKPVFSHGQLYVAISRVKSRAGLKI 1494
+ L + AMTI +SQG SL +V + +YVA+SR S LK+
Sbjct: 799 YGLSSKLAMTIARSQGLSLEKVAICFTADKLRLNSVYVAMSRTVSSRFLKM 849
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.359 0.162 0.586
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 147,991,032
Number of Sequences: 164201
Number of extensions: 5658222
Number of successful extensions: 28114
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 28097
Number of HSP's gapped (non-prelim): 17
length of query: 1520
length of database: 59,974,054
effective HSP length: 123
effective length of query: 1397
effective length of database: 39,777,331
effective search space: 55568931407
effective search space used: 55568931407
T: 11
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.8 bits)
S2: 73 (32.7 bits)
Lotus: description of TM0233.16