BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0233.11
(95 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RK25_PEA (P11892) 50S ribosomal protein CL25, chloroplast precursor 94 8e-20
CAR6_HUMAN (Q9BX69) Caspase recruitment domain protein 6 34 0.056
NCD_DROME (P20480) Claret segregational protein 33 0.16
TUL4_MOUSE (Q9JIL5) Tubby-like protein 4 (Tubby superfamily prot... 31 0.62
RAI1_HUMAN (Q7Z5J4) Retinoic acid induced protein 1 31 0.62
UL35_HCMVA (P16766) Hypothetical protein UL35 30 0.81
BASP_BOVIN (P80724) Brain acid soluble protein 1 (BASP1 protein)... 30 0.81
PGCA_RAT (P07897) Aggrecan core protein precursor (Cartilage-spe... 30 1.4
Z512_HUMAN (Q96ME7) Zinc finger protein 512 29 2.4
TUL4_HUMAN (Q9NRJ4) Tubby-like protein 4 (Tubby superfamily prot... 29 2.4
PGCA_MOUSE (Q61282) Aggrecan core protein precursor (Cartilage-s... 29 2.4
TEGU_HCMVA (P16785) Probable large tegument protein 28 5.2
NO20_MEDTR (P93329) Early nodulin 20 precursor (N-20) 28 5.2
MANB_ASPNG (Q9UUZ3) Beta-mannosidase precursor (EC 3.2.1.25) (Ma... 28 5.2
DPOL_HPBHE (P13846) P protein [Includes: DNA-directed DNA polyme... 28 5.2
PSD1_CAEEL (Q18115) 26S proteasome non-ATPase regulatory subunit... 27 6.8
MI13_HUMAN (Q8N3F8) Molecule interacting with Rab13 (MIRab13) (M... 27 6.8
DDF1_BOVIN (O97902) 130-kDa phosphatidylinositol 4,5-biphosphate... 27 6.8
PINH_ARATH (Q9XGW1) PINHEAD protein (ZWILLE protein) 27 8.9
GID_MYXXA (Q9S449) Protein gid homolog 27 8.9
>RK25_PEA (P11892) 50S ribosomal protein CL25, chloroplast precursor
Length = 104
Score = 93.6 bits (231), Expect = 8e-20
Identities = 54/102 (52%), Positives = 69/102 (66%), Gaps = 12/102 (11%)
Query: 4 LSSCSSIFGVAMAAAPP--------QMERR---GNGVIVCSSRPQKKATAHHRKSRPRKS 52
++S SSIFG ++ AP + ERR G +I CSSRPQKK+TAHH K+RPRKS
Sbjct: 1 MASVSSIFGCGVSMAPNSSLRNKAIRTERRSACGGLLIECSSRPQKKSTAHHMKTRPRKS 60
Query: 53 QPWDINRKPTVYAPLPPLPSDWTLVVSAADASSDEAVAVTPS 94
+ D NRKPTVYAPLPPLP D+T+V+ ADAS+ + P+
Sbjct: 61 RLSDRNRKPTVYAPLPPLPPDFTIVI-PADASTVDFTPPPPT 101
>CAR6_HUMAN (Q9BX69) Caspase recruitment domain protein 6
Length = 1037
Score = 34.3 bits (77), Expect = 0.056
Identities = 18/57 (31%), Positives = 28/57 (48%), Gaps = 2/57 (3%)
Query: 32 CSSRPQKKATAHHRKSRPRKSQPWDINRKPTVYAPLPPLPSDWTLVVSAADASSDEA 88
C S+P + + + ++P+ SQPW KP+ P PP P + S A A +A
Sbjct: 975 CQSQPSQTKPSPCKSTQPKPSQPWPPQSKPS--QPRPPQPKSSSTNPSQAKAHHSKA 1029
>NCD_DROME (P20480) Claret segregational protein
Length = 700
Score = 32.7 bits (73), Expect = 0.16
Identities = 27/76 (35%), Positives = 31/76 (40%), Gaps = 8/76 (10%)
Query: 19 PPQMERRGNGVIVCSSRPQKKATAHHRKSRPRKSQPWDIN----RKPTVYAP-LPPLPSD 73
P +ERRG G S P K HR R DIN K T AP LP +PS
Sbjct: 65 PQVLERRGGGARAASPEPMKLG---HRAKLRRSRSACDINELRGNKRTAAAPSLPSIPSK 121
Query: 74 WTLVVSAADASSDEAV 89
+ + A SS V
Sbjct: 122 VSRLGGALTVSSQRLV 137
>TUL4_MOUSE (Q9JIL5) Tubby-like protein 4 (Tubby superfamily
protein)
Length = 1547
Score = 30.8 bits (68), Expect = 0.62
Identities = 20/52 (38%), Positives = 24/52 (45%), Gaps = 5/52 (9%)
Query: 20 PQMERRGNGVIVCSSRPQKKATAHHRKSRPRKSQPWDINRKPTVYAPLPPLP 71
PQ+ G+ V+ S PQ+ A K P P I PT AP PPLP
Sbjct: 813 PQLAAEGDAVVF--SAPQEVQVA---KMNPPPPYPGTIPAAPTTAAPPPPLP 859
>RAI1_HUMAN (Q7Z5J4) Retinoic acid induced protein 1
Length = 1906
Score = 30.8 bits (68), Expect = 0.62
Identities = 18/56 (32%), Positives = 24/56 (42%)
Query: 26 GNGVIVCSSRPQKKATAHHRKSRPRKSQPWDINRKPTVYAPLPPLPSDWTLVVSAA 81
GNG RP+ T R S P+K++P N +P P P P + S A
Sbjct: 1253 GNGGDGKEERPEGSPTLFKRMSSPKKAKPTKGNGEPATKLPPPETPDACLKLASRA 1308
>UL35_HCMVA (P16766) Hypothetical protein UL35
Length = 640
Score = 30.4 bits (67), Expect = 0.81
Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 4/52 (7%)
Query: 5 SSCSSIFGVAMA--AAPPQMERRGNGVIVCSSRPQKKATAHHRKSRPRKSQP 54
SS S G++ A PP+ RR V + S P A +HHR+ R R+S P
Sbjct: 551 SSLSGSHGISSADLEQPPRQRRRMVSVTLFS--PYSVAYSHHRRHRRRRSPP 600
>BASP_BOVIN (P80724) Brain acid soluble protein 1 (BASP1 protein)
(Neuronal axonal membrane protein NAP-22)
Length = 226
Score = 30.4 bits (67), Expect = 0.81
Identities = 21/75 (28%), Positives = 29/75 (38%), Gaps = 4/75 (5%)
Query: 17 AAPPQMERRGNGVIVCSSRPQKKATAHHRKSRPRKSQPWDINRKPTVYAPLPPLPSDWTL 76
AAP E + +G S+P A K P ++ T A P P+D
Sbjct: 154 AAPAAQETKSDGAPASDSKPSSTEAAPSSKETPAATEA----PSSTPKAQAPAAPADEVK 209
Query: 77 VVSAADASSDEAVAV 91
A+SD+ VAV
Sbjct: 210 PAETPAANSDQTVAV 224
>PGCA_RAT (P07897) Aggrecan core protein precursor
(Cartilage-specific proteoglycan core protein) (CSPCP)
Length = 2124
Score = 29.6 bits (65), Expect = 1.4
Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Query: 47 SRPRKSQPWDINRKPTVYAPLPPLPSDWTLVVSAADASSDE-AVAVTPSA 95
+ P K W+ P +PLP +P W V AA+ ++ + + PSA
Sbjct: 731 TEPEKQTEWEPAYTPVGTSPLPGIPPTWLPTVPAAEEHTESPSASQEPSA 780
>Z512_HUMAN (Q96ME7) Zinc finger protein 512
Length = 567
Score = 28.9 bits (63), Expect = 2.4
Identities = 19/48 (39%), Positives = 24/48 (49%), Gaps = 6/48 (12%)
Query: 36 PQKKATAHHRKSRPR-----KSQPWDINRKPTVYAPLPPLPSDWTLVV 78
PQKK + RK RP+ KSQ I ++P VYA L W L +
Sbjct: 117 PQKKHKLYGRKQRPKTQPNPKSQARRIRKEPPVYA-AGSLEEQWYLEI 163
>TUL4_HUMAN (Q9NRJ4) Tubby-like protein 4 (Tubby superfamily
protein)
Length = 1544
Score = 28.9 bits (63), Expect = 2.4
Identities = 19/52 (36%), Positives = 23/52 (43%), Gaps = 5/52 (9%)
Query: 20 PQMERRGNGVIVCSSRPQKKATAHHRKSRPRKSQPWDINRKPTVYAPLPPLP 71
PQ+ G+ V+ S PQ+ K P P I PT AP PPLP
Sbjct: 810 PQLAAEGDAVVF--SAPQEVQVT---KINPPPPYPGTIPAAPTTAAPPPPLP 856
>PGCA_MOUSE (Q61282) Aggrecan core protein precursor
(Cartilage-specific proteoglycan core protein) (CSPCP)
Length = 2132
Score = 28.9 bits (63), Expect = 2.4
Identities = 20/76 (26%), Positives = 30/76 (39%), Gaps = 8/76 (10%)
Query: 26 GNGVIVCSSRPQKKATAHHRKSRPRKSQPWDINRKPTVYAPLPPLPSDWTLVVSAAD--- 82
G GV P K + + PRK W+ P +P P +P W + AA+
Sbjct: 715 GPGVDAVPLEP-KTTEVPYFTTEPRKQTEWEPAYTPVGTSPQPGIPPTWLPTLPAAEEHT 773
Query: 83 ----ASSDEAVAVTPS 94
AS + + + PS
Sbjct: 774 ESPSASEEPSASAVPS 789
>TEGU_HCMVA (P16785) Probable large tegument protein
Length = 2241
Score = 27.7 bits (60), Expect = 5.2
Identities = 22/90 (24%), Positives = 35/90 (38%), Gaps = 12/90 (13%)
Query: 5 SSCSSIFGVAMAAAPPQMERRGNGVIVCSSRPQKKATAHHRKSRPRKSQPWDINRKPTVY 64
SS S+I + ++A P G G + R Q+ ++ K
Sbjct: 316 SSPSAIAAASSSSAVPPSYSCGEGALPALGRYQQLVDEVEQEL------------KALTL 363
Query: 65 APLPPLPSDWTLVVSAADASSDEAVAVTPS 94
PLP S WTL + ++ ++ A A PS
Sbjct: 364 PPLPANTSAWTLHAAGTESGANAATATAPS 393
>NO20_MEDTR (P93329) Early nodulin 20 precursor (N-20)
Length = 268
Score = 27.7 bits (60), Expect = 5.2
Identities = 19/65 (29%), Positives = 27/65 (41%), Gaps = 3/65 (4%)
Query: 33 SSRPQKKATAHHRKSRPRKSQPWDINRK---PTVYAPLPPLPSDWTLVVSAADASSDEAV 89
S P+ H RK P P K P+ L P PSD ++ + + SDE+
Sbjct: 176 SPSPRSTPIPHPRKRSPASPSPSPSLSKSPSPSESPSLAPSPSDSVASLAPSSSPSDESP 235
Query: 90 AVTPS 94
+ PS
Sbjct: 236 SPAPS 240
>MANB_ASPNG (Q9UUZ3) Beta-mannosidase precursor (EC 3.2.1.25)
(Mannanase) (Mannase)
Length = 931
Score = 27.7 bits (60), Expect = 5.2
Identities = 13/37 (35%), Positives = 18/37 (48%), Gaps = 3/37 (8%)
Query: 49 PRKSQPWDINRKPTVYAPLPPLPSDWTLVVSAADASS 85
P +SQPW +N + PLP P T+ + D S
Sbjct: 238 PDQSQPWVVNASIDILGPLPAKP---TMSIEVRDTHS 271
>DPOL_HPBHE (P13846) P protein [Includes: DNA-directed DNA
polymerase (EC 2.7.7.7); RNA-directed DNA polymerase (EC
2.7.7.49); Ribonuclease H (EC 3.1.26.4)]
Length = 788
Score = 27.7 bits (60), Expect = 5.2
Identities = 15/43 (34%), Positives = 22/43 (50%), Gaps = 5/43 (11%)
Query: 24 RRGNGVIVCSSRPQKKA-----TAHHRKSRPRKSQPWDINRKP 61
++G GV+ SR + T+HHR +R R S D R+P
Sbjct: 303 QKGQGVLQAISREPSETRRNGTTSHHRVARCRTSSVEDFTRRP 345
>PSD1_CAEEL (Q18115) 26S proteasome non-ATPase regulatory subunit 1
(26S proteasome regulatory subunit rpn-2)
Length = 965
Score = 27.3 bits (59), Expect = 6.8
Identities = 23/86 (26%), Positives = 30/86 (34%), Gaps = 4/86 (4%)
Query: 6 SCSSIFGVAMAAAPPQMERRGNGVIVCSSRPQKKATAHHRKSRPRKSQPWDIN----RKP 61
S SS G A AA +ME G P+K HR P + P
Sbjct: 846 SSSSTSGTAPAAEDEKMEVDQPGKSKKEKAPEKDTKPLHRLQNPARVIPAQRQLISISDA 905
Query: 62 TVYAPLPPLPSDWTLVVSAADASSDE 87
Y+P+ PL +V D +E
Sbjct: 906 RAYSPMKPLYKGGIIVADRVDKEREE 931
>MI13_HUMAN (Q8N3F8) Molecule interacting with Rab13 (MIRab13)
(MICAL-like protein 1)
Length = 863
Score = 27.3 bits (59), Expect = 6.8
Identities = 17/55 (30%), Positives = 23/55 (40%), Gaps = 4/55 (7%)
Query: 44 HRKSRPRKSQPW----DINRKPTVYAPLPPLPSDWTLVVSAADASSDEAVAVTPS 94
H +S P+ PW + T P P PS L + A+ S E + TPS
Sbjct: 452 HPESTPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLALHASRLSHSEPPSATPS 506
>DDF1_BOVIN (O97902) 130-kDa phosphatidylinositol
4,5-biphosphate-dependent ARF1 GTPase-activating protein
(PIP2-dependent ARF1 GAP) (ADP-ribosylation
factor-directed GTPase-activating protein 1) (ARF
GTPase-activating protein 1) (Development and differen
Length = 1129
Score = 27.3 bits (59), Expect = 6.8
Identities = 11/22 (50%), Positives = 14/22 (63%)
Query: 49 PRKSQPWDINRKPTVYAPLPPL 70
P+K P D+ KPT AP PP+
Sbjct: 937 PQKPPPGDLPPKPTELAPKPPI 958
>PINH_ARATH (Q9XGW1) PINHEAD protein (ZWILLE protein)
Length = 988
Score = 26.9 bits (58), Expect = 8.9
Identities = 19/59 (32%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
Query: 33 SSRPQKKATAHHRKSRPRKSQPWDINRKPTVYAPLPPLPSDWTLVVSAADASSDEAVAV 91
S P K + R RKS D+ +P+ PP PS T + + A++ E VAV
Sbjct: 57 SPSPPSKNRSRRRNRGGRKSDQGDVCMRPSSRPRKPPPPSQ-TTSSAVSVATAGEIVAV 114
>GID_MYXXA (Q9S449) Protein gid homolog
Length = 457
Score = 26.9 bits (58), Expect = 8.9
Identities = 12/32 (37%), Positives = 19/32 (58%)
Query: 12 GVAMAAAPPQMERRGNGVIVCSSRPQKKATAH 43
G+A Q+ RRG V++ +PQK++ AH
Sbjct: 14 GLAGTECAYQLSRRGVPVVLREMKPQKRSPAH 45
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.314 0.124 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,746,056
Number of Sequences: 164201
Number of extensions: 386371
Number of successful extensions: 1270
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1259
Number of HSP's gapped (non-prelim): 21
length of query: 95
length of database: 59,974,054
effective HSP length: 71
effective length of query: 24
effective length of database: 48,315,783
effective search space: 1159578792
effective search space used: 1159578792
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0233.11