BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0228b.2
(357 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
APG_ARATH (P40602) Anter-specific proline-rich protein APG precu... 146 7e-35
APG_BRANA (P40603) Anter-specific proline-rich protein APG (Prot... 135 2e-31
EST_HEVBR (Q7Y1X1) Esterase precursor (EC 3.1.1.-) (Early nodule... 74 6e-13
LIP1_PHOLU (P40601) Lipase 1 precursor (EC 3.1.1.3) (Triacylglyc... 40 0.012
GCC1_MOUSE (Q9D4H2) GRIP and coiled-coil domain-containing prote... 34 0.65
UL17_HHV7J (P52360) Protein U64 32 2.5
RUVA_RICPR (Q9ZDE6) Holliday junction DNA helicase ruvA 32 3.2
LEU3_BUCRP (P48572) 3-isopropylmalate dehydrogenase (EC 1.1.1.85... 32 3.2
PT1_BACST (P42014) Phosphoenolpyruvate-protein phosphotransferas... 31 5.5
PT1_BACSI (O83018) Phosphoenolpyruvate-protein phosphotransferas... 31 5.5
FLGI_BUCBP (Q89AH5) Flagellar P-ring protein precursor (Basal bo... 31 5.5
UGGG_SCHPO (Q09140) UDP-glucose:glycoprotein glucosyltransferase... 30 7.2
PEPX_STRMC (Q8L376) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11) ... 30 7.2
>APG_ARATH (P40602) Anter-specific proline-rich protein APG
precursor
Length = 534
Score = 146 bits (369), Expect = 7e-35
Identities = 97/322 (30%), Positives = 164/322 (50%), Gaps = 27/322 (8%)
Query: 28 SQAVPCLFVFGDSLADSGNNNNLPTLSKANFLPYGIDFP-TGPTGRYTNGLNPIDKLAQI 86
++ +P +F FGDS+ D+GNNNNL T K+N+ PYG+DF TGR++NG+ D LA+
Sbjct: 199 NKTIPAVFFFGDSVFDTGNNNNLETKIKSNYRPYGMDFKFRVATGRFSNGMVASDYLAKY 258
Query: 87 LGFEKFIPPFAN--LSGSDILKGVNYASGSAGIRQETGTNLGTNVNMGLQLQHHRTIVSQ 144
+G ++ +P + + + +D+L GV++ASG AG T + + M QL + + + +
Sbjct: 259 MGVKEIVPAYLDPKIQPNDLLTGVSFASGGAGYNPTT-SEAANAIPMLDQLTYFQDYIEK 317
Query: 145 IST---------KLGGFHKAVNYLTQCLYYVYIGTNDYEQNYFLPDLFNTSRTYTPEQYA 195
++ KL G K +++ + V G+ND YF + Y
Sbjct: 318 VNRLVRQEKSQYKLAGLEKTNQLISKGVAIVVGGSNDLIITYFGSGAQRLKNDI--DSYT 375
Query: 196 KVLTHQLSHYLKALHHVGARKTVVVSLDRLGCIP--KVFVNGSCIEKQNAAAFLFNDQLK 253
++ + ++ L+ GAR+ V+ LGC+P ++ C E+ N A+ LFN +L
Sbjct: 376 TIIADSAASFVLQLYGYGARRIGVIGTPPLGCVPSQRLKKKKICNEELNYASQLFNSKLL 435
Query: 254 SLVDRFNKKTLKGSKFIFINSTAIIHD-----KSNGFKFTNAPCCTTN--ERGKCILNGT 306
++ + + KTL S F++++ II + GF+ T PCC T G T
Sbjct: 436 LILGQLS-KTLPNSTFVYMDIYTIISQMLETPAAYGFEETKKPCCKTGLLSAGALCKKST 494
Query: 307 P--CQNRTEYVFWDGIHTSEAA 326
C N + Y+FWDG+H ++ A
Sbjct: 495 SKICPNTSSYLFWDGVHPTQRA 516
>APG_BRANA (P40603) Anter-specific proline-rich protein APG (Protein
CEX) (Fragment)
Length = 449
Score = 135 bits (340), Expect = 2e-31
Identities = 97/323 (30%), Positives = 156/323 (48%), Gaps = 33/323 (10%)
Query: 28 SQAVPCLFVFGDSLADSGNNNNLPTLSKANFLPYGIDFPTG-PTGRYTNGLNPIDKLAQI 86
++ +P +F FGDS+ D+GNNNNL T K N+ PYG+DFP G TGR++NG D +++
Sbjct: 120 NKTIPAVFFFGDSIFDTGNNNNLDTKLKCNYRPYGMDFPMGVATGRFSNGRVASDYISKY 179
Query: 87 LGFEKFIPPFAN--------LSGSDILKGVNYASGSAGIRQETGTNLGTNVNMGLQLQHH 138
LG ++ +P + + L SD+L GV++ASG AG +T + + QL +
Sbjct: 180 LGVKEIVPAYVDKKLQQNNELQQSDLLTGVSFASGGAGYLPQTSESWKVTTMLD-QLTYF 238
Query: 139 RTIVSQISTKLGGFHKAVNYLTQCLYYVYIGTNDYEQNYFLPDLFNTSRTYTPEQYAKVL 198
+ ++ KL G K +++ V G+ND YF + + + ++
Sbjct: 239 QDYKKRMK-KLVGKKKTKKIVSKGAAIVVAGSNDLIYTYFGNGAQHLKNDV--DSFTTMM 295
Query: 199 THQLSHYLKALHHVGARKTVVVSLDRLGCIP--KVFVNGSCIEKQNAAAFLFNDQLKSLV 256
+ ++ L+ GAR+ V+ +GC P +V C E N AA LFN +L ++
Sbjct: 296 ADSAASFVLQLYGYGARRIGVIGTPPIGCTPSQRVKKKKICNEDLNYAAQLFNSKLVIIL 355
Query: 257 DRFNKKTLKGSKFIF-----INSTAIIHDKSNGFKFTNAPCCTTNERGKCILNGTPCQNR 311
+ + KTL S ++ I S + + GF+ PCC G C+ R
Sbjct: 356 GQLS-KTLPNSTIVYGDIYSIFSKMLESPEDYGFEEIKKPCCKIG----LTKGGVFCKER 410
Query: 312 T--------EYVFWDGIHTSEAA 326
T Y+FWDG+H S+ A
Sbjct: 411 TLKNMSNASSYLFWDGLHPSQRA 433
>EST_HEVBR (Q7Y1X1) Esterase precursor (EC 3.1.1.-) (Early
nodule-specific protein homolog) (Latex allergen Hev b
13)
Length = 391
Score = 73.9 bits (180), Expect = 6e-13
Identities = 83/362 (22%), Positives = 151/362 (40%), Gaps = 60/362 (16%)
Query: 10 VLSLVLMVACMQHSVLGNSQAVPCLFVFGDSLADSGNNNNLPTLSKANFLP----YGIDF 65
+++L ++ + + + P +F FGDS +D+G A F P YG F
Sbjct: 11 IITLSFLLCMLSLAYASETCDFPAIFNFGDSNSDTGGK-------AAAFYPLNPPYGETF 63
Query: 66 PTGPTGRYTNGLNPIDKLAQILGFEKFIPPFANLSGSDILKGVNYASGSAGIRQET---- 121
TGRY++G ID +A+ ++ P+ + GS+ G ++A+ + I+ T
Sbjct: 64 FHRSTGRYSDGRLIIDFIAESFNL-PYLSPYLSSLGSNFKHGADFATAGSTIKLPTTIIP 122
Query: 122 GTNLGTNVNMGLQLQHHRTIV--SQISTKLGGFHKAV----NYLTQCLYYVYIGTNDYEQ 175
+ + +Q R + SQ + GG + Y + LY IG ND +
Sbjct: 123 AHGGFSPFYLDVQYSQFRQFIPRSQFIRETGGIFAELVPEEYYFEKALYTFDIGQNDLTE 182
Query: 176 NYFLPDLFNTSRTYTPEQYAKVLTHQLSHYLKALHHVGARKTVVVSLDRLGCIPKVFV-- 233
+ + + T P+ L + S +K ++ +GAR + + +GC+ +
Sbjct: 183 GFLNLTVEEVNAT-VPD-----LVNSFSANVKKIYDLGARTFWIHNTGPIGCLSFILTYF 236
Query: 234 ------NGSCIEKQNAAAFLFNDQLKSLVDRFNKKTLKGSKFIFINSTAIIHD-----KS 282
+ C + N A FN +LK +V + +K L + F+ ++ ++ + +
Sbjct: 237 PWAEKDSAGCAKAYNEVAQHFNHKLKEIVAQL-RKDLPLATFVHVDIYSVKYSLFSEPEK 295
Query: 283 NGFKFTNAPCC-----------------TTNERGKCILNGTPCQNRTEYVFWDGIHTSEA 325
+GF+F CC T + G I+ G+ C + V WDG H +EA
Sbjct: 296 HGFEFPLITCCGYGGKYNFSVTAPCGDTVTADDGTKIVVGS-CACPSVRVNWDGAHYTEA 354
Query: 326 AN 327
AN
Sbjct: 355 AN 356
>LIP1_PHOLU (P40601) Lipase 1 precursor (EC 3.1.1.3)
(Triacylglycerol lipase)
Length = 645
Score = 39.7 bits (91), Expect = 0.012
Identities = 52/220 (23%), Positives = 79/220 (35%), Gaps = 71/220 (32%)
Query: 23 SVLGNSQAVPCLFVFGDSLADSGNNNNLPTLSKANFLPYGIDFPTGPTGRYTNGLNPIDK 82
S + N+ A L+VFGDSL+D GNN Y +D G + N D
Sbjct: 17 SAISNAHAYNNLYVFGDSLSDGGNNGR-----------YTVDGINGTESKLYN-----DF 60
Query: 83 LAQILGFEKFIPPFANLSGSDILKGVNYASGSAGIRQETGTNLGTNVNMGLQLQHHRTIV 142
+AQ LG E + G NYA+G A T V
Sbjct: 61 IAQQLGIELV---------NSKKGGTNYAAGGA------------------------TAV 87
Query: 143 SQISTKLGGFHKAVNYLT--------QCLYYVYIGTNDYEQNYFLPDLFNTSRTYTPEQY 194
+ ++ K + + YL +Y +IG ND + + P
Sbjct: 88 ADLNNKHNTQDQVMGYLASHSNRADHNGMYVHWIGGNDVD-----------AALRNPADA 136
Query: 195 AKVLTHQ---LSHYLKALHHVGARKTVVVSLDRLGCIPKV 231
K++T S + AL + GA +V ++ +G PK+
Sbjct: 137 QKIITESAMAASSQVHALLNAGAGLVIVPTVPDVGMTPKI 176
>GCC1_MOUSE (Q9D4H2) GRIP and coiled-coil domain-containing protein
1 (Golgi coiled coil protein 1)
Length = 778
Score = 33.9 bits (76), Expect = 0.65
Identities = 18/61 (29%), Positives = 33/61 (53%), Gaps = 2/61 (3%)
Query: 206 LKALHHVGARKT-VVVSLDRLGCIPKVFVNGSCIEKQNAAAFLFNDQLKSLVDRFNKKTL 264
LK L V ARK+ V + +++L C P++ N + + A A + +LK L + F + +
Sbjct: 439 LKKLLQVAARKSQVTLDVEKL-CDPEIMANSEAADGEKATALYYQQELKQLKEEFERYKM 497
Query: 265 K 265
+
Sbjct: 498 R 498
>UL17_HHV7J (P52360) Protein U64
Length = 439
Score = 32.0 bits (71), Expect = 2.5
Identities = 32/126 (25%), Positives = 49/126 (38%), Gaps = 20/126 (15%)
Query: 152 FHKAVNYLTQCLYYV---------YIGTNDYEQNYFLPDLFNTSRTYTPEQYAKVLTHQL 202
F NYL + Y+ I TN QN LP FN+ +
Sbjct: 254 FSSITNYLDRVNTYLDEIAERTFRCITTNMEIQNRHLPQKFNSHFQ---------IEFNC 304
Query: 203 SHYLKALHHVGARKTVVVSLDRLGCIPKVFVNGSCIEKQNAAAFLFNDQLKSLVDRFNKK 262
+H + + AR ++SLDR C+ K + +K+ ++ N+ L+D K
Sbjct: 305 THLISGMEL--ARDFWILSLDRNSCVLKAMASHFLHKKKGRSSLSSNEFWADLIDCTTGK 362
Query: 263 TLKGSK 268
TL G K
Sbjct: 363 TLYGEK 368
>RUVA_RICPR (Q9ZDE6) Holliday junction DNA helicase ruvA
Length = 199
Score = 31.6 bits (70), Expect = 3.2
Identities = 17/68 (25%), Positives = 34/68 (50%)
Query: 190 TPEQYAKVLTHQLSHYLKALHHVGARKTVVVSLDRLGCIPKVFVNGSCIEKQNAAAFLFN 249
TP + + + KA+ VGA+ + L+ I K+F + + I+ N ++ N
Sbjct: 93 TPTDIQIAINNDDRNIFKAISGVGAKLAERIMLELKDKITKIFSSSAIIKDSNISSIAIN 152
Query: 250 DQLKSLVD 257
+ +K+LV+
Sbjct: 153 EVMKALVN 160
>LEU3_BUCRP (P48572) 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
(Beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
Length = 363
Score = 31.6 bits (70), Expect = 3.2
Identities = 32/110 (29%), Positives = 50/110 (45%), Gaps = 12/110 (10%)
Query: 18 ACMQHSVLGNSQ---AVPCLFVFGDSLAD-----SGNNNNLPTLSKANFLPYGIDFPTGP 69
ACMQ ++ N + C +FGD ++D +G+ LP+ S N +G+ P G
Sbjct: 229 ACMQ--IIKNPSQFDVLLCSNIFGDIISDECAMITGSIGMLPSAS-LNSKKFGLYEPAGG 285
Query: 70 TGRYTNGLNPIDKLAQILGFEKFIPPFANLSG-SDILKGVNYASGSAGIR 118
+ G N + +AQIL I NL+ +D + Y + AG R
Sbjct: 286 SAPDIEGKNIANPIAQILSLSMLIRHSMNLNEIADKIDSSVYRALKAGYR 335
>PT1_BACST (P42014) Phosphoenolpyruvate-protein phosphotransferase
(EC 2.7.3.9) (Phosphotransferase system, enzyme I)
Length = 578
Score = 30.8 bits (68), Expect = 5.5
Identities = 29/120 (24%), Positives = 42/120 (34%), Gaps = 14/120 (11%)
Query: 94 PPFANLSGSDILKGVNYASGSAGIRQETGTNLGTNVNMGLQLQHHRTIVSQISTKLGGFH 153
P A L K + A +RQ G V M +++ + Q F
Sbjct: 397 PMIATLDEFRQAKAILLEEKEALLRQGVAVADGIEVGMMVEIPAAAVMADQ-------FA 449
Query: 154 KAVNYLTQCLYYVYIGTNDYEQNYFLPDLFNTSRTYTPEQYAKVLTHQLSHYLKALHHVG 213
K V++ + IGTND Q D N Y + Y + +SH + A H G
Sbjct: 450 KEVDFFS-------IGTNDLIQYTMAADRMNERVAYLYQPYNPAILRLISHVIDAAHREG 502
>PT1_BACSI (O83018) Phosphoenolpyruvate-protein phosphotransferase
(EC 2.7.3.9) (Phosphotransferase system, enzyme I)
Length = 578
Score = 30.8 bits (68), Expect = 5.5
Identities = 29/120 (24%), Positives = 42/120 (34%), Gaps = 14/120 (11%)
Query: 94 PPFANLSGSDILKGVNYASGSAGIRQETGTNLGTNVNMGLQLQHHRTIVSQISTKLGGFH 153
P A L K + A +RQ G V M +++ + Q F
Sbjct: 397 PMIATLDEFRQAKAILLEEKEALLRQGVAVADGIEVGMMVEIPAAAVMADQ-------FA 449
Query: 154 KAVNYLTQCLYYVYIGTNDYEQNYFLPDLFNTSRTYTPEQYAKVLTHQLSHYLKALHHVG 213
K V++ + IGTND Q D N Y + Y + +SH + A H G
Sbjct: 450 KEVDFFS-------IGTNDLIQYTMAADRMNERVAYLYQPYNPAILRLISHVIDAAHREG 502
>FLGI_BUCBP (Q89AH5) Flagellar P-ring protein precursor (Basal body
P-ring protein)
Length = 375
Score = 30.8 bits (68), Expect = 5.5
Identities = 36/126 (28%), Positives = 58/126 (45%), Gaps = 7/126 (5%)
Query: 78 NPIDKLAQ---ILGFEKFIPPFANLSGSDILKGVNYASGSAGIRQETGTNLGTNVNMGLQ 134
N I +AQ I+ + + F N+ ++ L +G A I +E T+ G N + LQ
Sbjct: 135 NKIYAVAQGNIIINDKNSVERFKNILVNNHLNNGMIING-ATIEREMHTDFGKNETLNLQ 193
Query: 135 LQHHR-TIVSQISTKLG-GFHKAVNYLTQCLYYVYIGTNDYEQNYFLPDLFNTSRTYTPE 192
L + T+ +IS K+ + K+ L + V I N+ EQ L + N + P
Sbjct: 194 LNNEDFTVAQEISKKINMQYPKSAVALNSKIIQVCIPNNNIEQVEMLATIQNIN-IPIPI 252
Query: 193 QYAKVL 198
Q AK+L
Sbjct: 253 QDAKIL 258
>UGGG_SCHPO (Q09140) UDP-glucose:glycoprotein glucosyltransferase
precursor (EC 2.4.1.-) (UDP--Glc:glycoprotein
glucosyltransferase) (UGT)
Length = 1448
Score = 30.4 bits (67), Expect = 7.2
Identities = 22/93 (23%), Positives = 39/93 (41%), Gaps = 3/93 (3%)
Query: 228 IPKVFVNGSCIEKQNAAAFLFNDQLKS---LVDRFNKKTLKGSKFIFINSTAIIHDKSNG 284
I +++NG ++ + AF +K + DRF +K SK + I + ++ +
Sbjct: 337 INTIWLNGLSLDLEETDAFSILSLIKKEKDMFDRFEALGIKSSKVLDIVTNEAFANEDSD 396
Query: 285 FKFTNAPCCTTNERGKCILNGTPCQNRTEYVFW 317
FKF C E K I ++ +Y W
Sbjct: 397 FKFVKFHCQDDIEDWKAIHWVNEIESNPKYDNW 429
>PEPX_STRMC (Q8L376) Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11)
(X-Pro dipeptidyl-peptidase) (X-prolyl-dipeptidyl
aminopeptidase) (X-PDAP)
Length = 763
Score = 30.4 bits (67), Expect = 7.2
Identities = 33/121 (27%), Positives = 56/121 (46%), Gaps = 10/121 (8%)
Query: 141 IVSQISTKLGGFHKAVNYLTQCLYYVYIGTNDYEQNYFLPDLFNTSRTYTPEQYAKVLTH 200
+++ T L F + LT ++Y T + F+P + T+ T E+ A + +
Sbjct: 58 LIADFETDLLTFFNSERPLTADIFY----TISLQLLGFIPHVDFTNTTDFLEKIAFPINY 113
Query: 201 QLSHYLKALHH--VGARKTVVVSLDRL---GCIPKVFVNGSCIEKQNAAAFLFNDQLKSL 255
Q H L+AL+H V +K+ + LD L G IP V N ++ A F D ++ +
Sbjct: 114 QKGHILEALYHLLVSRQKSGMTLLDDLISKGLIP-VDNNYHFFNGKSLATFDTTDLIREV 172
Query: 256 V 256
V
Sbjct: 173 V 173
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.136 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,367,771
Number of Sequences: 164201
Number of extensions: 1857026
Number of successful extensions: 3806
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 3785
Number of HSP's gapped (non-prelim): 15
length of query: 357
length of database: 59,974,054
effective HSP length: 111
effective length of query: 246
effective length of database: 41,747,743
effective search space: 10269944778
effective search space used: 10269944778
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0228b.2