BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0227.9
         (273 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YK75_MYCTU (Q10683) Hypothetical protein Rv2075c/MT2135                38  0.023
PLC1_YEAST (P32383) 1-phosphatidylinositol-4,5-bisphosphate phos...    33  0.57

>YK75_MYCTU (Q10683) Hypothetical protein Rv2075c/MT2135
          Length = 487

 Score = 38.1 bits (87), Expect = 0.023
 Identities = 34/126 (26%), Positives = 57/126 (44%), Gaps = 17/126 (13%)

Query: 3   LPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDM------- 55
           +P  E  WL THNSF +   +++      A +NQ+ S+  QL   VR L LD+       
Sbjct: 112 VPLRETQWLGTHNSFNSLSDSFTVSH---ADSNQQLSLAQQLDIDVRALELDLHYLPRLE 168

Query: 56  WDYEDTIWLCRGPCTKYT----TFQPAL-NVLKEVRVFLVT--HPTEIITIFIDDHVTSG 108
                 + +C G   K      T +P L  VL ++  +L    H  E+I ++++D + + 
Sbjct: 169 GHGAPGVTVCHGLGPKNANLGCTVEPLLATVLPQIANWLNAPGHTEEVILLYLEDQLKNA 228

Query: 109 NGVNKV 114
           +    V
Sbjct: 229 SAYESV 234


>PLC1_YEAST (P32383) 1-phosphatidylinositol-4,5-bisphosphate
           phosphodiesterase 1 (EC 3.1.4.11) (Phosphoinositide
           phospholipase C) (PLC-1) (Phospholipase C-1)
          Length = 869

 Score = 33.5 bits (75), Expect = 0.57
 Identities = 32/163 (19%), Positives = 69/163 (41%), Gaps = 17/163 (10%)

Query: 4   PFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMWDYEDTIW 63
           P N Y   ++HN++             +A T   +     L+ G R + +D+WD E+   
Sbjct: 385 PLNHYFIASSHNTYLL--------GKQIAETPSVEGYIQVLQQGCRCVEIDIWDGENGPV 436

Query: 64  LCRGPCTKYTTFQPALNVLKEVRVFLVTHPTEIITIFIDDHVTSGNGVNKVFDKARLRKF 123
           +C G  T     +  + V+K+       +P  II++ I+ +  +    + +  +    + 
Sbjct: 437 VCHGFLTSAIPLKTVIRVIKKYAFITSPYPL-IISLEINCNKDNQKLASLIMREVLAEQL 495

Query: 124 WFPVSKMPKNGSDWPTVKTMIRKNYRLIVFTSNASREASEGIA 166
           +F  ++  K     P+ + +  K    I+  S  + EA+ G++
Sbjct: 496 YFVGTRTDK----LPSPRELKHK----ILLKSKKTSEATRGLS 530


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.320    0.136    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,165,147
Number of Sequences: 164201
Number of extensions: 1418880
Number of successful extensions: 2504
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2503
Number of HSP's gapped (non-prelim): 2
length of query: 273
length of database: 59,974,054
effective HSP length: 109
effective length of query: 164
effective length of database: 42,076,145
effective search space: 6900487780
effective search space used: 6900487780
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 65 (29.6 bits)