BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0227.12
(414 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UBE4_CAEEL (Q09349) Probable ubiquitin conjugation factor E4 44 8e-04
UFD2_SCHPO (Q9HE05) Ubiquitin conjugation factor E4 (Ubiquitin f... 41 0.005
TM26_PIG (O77666) Tripartite motif-containing protein 26 (Zinc f... 41 0.006
NF7B_XENLA (Q92021) Nuclear factor 7, brain (xnf7) (xnf7-B) 41 0.006
NF7O_XENLA (Q91431) Nuclear factor 7, ovary (xnf7-O) 39 0.019
TM30_MOUSE (P15533) Tripartite motif-containing protein 30 (Down... 39 0.024
TRM4_HUMAN (Q9C037) Tripartite motif-containing protein 4 37 0.071
UB4B_MOUSE (Q9ES00) Ubiquitin conjugation factor E4 B (Ubiquitin... 37 0.093
UB4B_HUMAN (O95155) Ubiquitin conjugation factor E4 B (Ubiquitin... 37 0.093
UB4A_HUMAN (Q14139) Ubiquitin conjugation factor E4 A 37 0.12
TM47_MOUSE (Q8C0E3) Tripartite motif-containing protein 47 36 0.16
TRM5_HUMAN (Q9C035) Tripartite motif-containing protein 5 (EC 6.... 36 0.21
TM26_MOUSE (Q99PN3) Tripartite motif-containing protein 26 (Zinc... 36 0.21
TM22_HUMAN (Q8IYM9) Tripartite motif-containing protein 22 (RING... 36 0.21
VAC8_YEAST (P39968) Vacuolar protein 8 35 0.35
TM47_HUMAN (Q96LD4) Tripartite motif-containing protein 47 (RING... 35 0.35
TM26_RAT (P62603) Tripartite motif-containing protein 26 (Zinc f... 35 0.35
RUVC_RHILO (Q98F72) Crossover junction endodeoxyribonuclease ruv... 35 0.46
N192_YEAST (P47054) Nucleoporin NUP192 (Nuclear pore protein NUP... 35 0.46
A33_PLEWA (Q02084) Zinc-binding protein A33 35 0.46
>UBE4_CAEEL (Q09349) Probable ubiquitin conjugation factor E4
Length = 980
Score = 43.9 bits (102), Expect = 8e-04
Identities = 27/73 (36%), Positives = 39/73 (52%), Gaps = 3/73 (4%)
Query: 14 IPYHYRCPISLELMRDPVTVCTGQTYDRSSIESWV-NTGNTTCPVTRAPLTEFTLIPNHT 72
+P ++ PI +M DPV + +G DR+ IE + +T N P RAPL+ L P+
Sbjct: 906 VPEEFKDPIMDAIMVDPVKLPSGHVMDRAVIERHLLSTPNN--PFNRAPLSHNELSPDSE 963
Query: 73 LRRLIQEWCVANR 85
L+ IQEW R
Sbjct: 964 LKAKIQEWICQKR 976
>UFD2_SCHPO (Q9HE05) Ubiquitin conjugation factor E4 (Ubiquitin fusion
degradation protein 2) (UB fusion protein 2)
Length = 1010
Score = 41.2 bits (95), Expect = 0.005
Identities = 28/81 (34%), Positives = 45/81 (54%), Gaps = 3/81 (3%)
Query: 6 EPLDLGIQIPYHYRCPISLELMRDPVTVC-TGQTYDRSSIESWVNTGNTTCPVTRAPLTE 64
E D+G IP ++ P+ +M+DPV + +G + DRS+I++ + + T P R PLT
Sbjct: 924 EEEDMG-DIPDYFLDPLMFTIMKDPVVLPRSGISIDRSTIKAHL-LSDATDPFNRTPLTL 981
Query: 65 FTLIPNHTLRRLIQEWCVANR 85
+ PN TLR I + + R
Sbjct: 982 DDVTPNDTLREEINTFLKSKR 1002
>TM26_PIG (O77666) Tripartite motif-containing protein 26 (Zinc
finger protein 173)
Length = 545
Score = 40.8 bits (94), Expect = 0.006
Identities = 27/87 (31%), Positives = 41/87 (47%), Gaps = 13/87 (14%)
Query: 20 CPISLELMRDPVTVCTGQTYDRSSIESW--VNTGNTTCPVTRAPLTEFTLIPNHTLRRLI 77
C I L+ +RDPVT+ G + RS V+ G CP+ + P T+ + P
Sbjct: 16 CSICLDYLRDPVTIDCGHVFCRSCTTDIHPVSGGRPVCPLCKKPFTKENIRP-------- 67
Query: 78 QEWCVANRAFGVER--IPTPKQPAEPA 102
W +A+ +ER + T +QP E A
Sbjct: 68 -VWQLASLVENIERLKVDTGRQPGEVA 93
>NF7B_XENLA (Q92021) Nuclear factor 7, brain (xnf7) (xnf7-B)
Length = 609
Score = 40.8 bits (94), Expect = 0.006
Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
Query: 20 CPISLELMRDPVTVCTGQTYDRSSIE-SWVNTGNTTCPVTRAPLTEFTLIPNHTLRRLIQ 78
CP+ +EL +DPV V G + RS I+ +W + CP R +T+ N L L +
Sbjct: 145 CPLCVELFKDPVMVACGHNFCRSCIDKAWEGQSSFACPECRESITDRKYTINRVLANLAK 204
Query: 79 E 79
+
Sbjct: 205 K 205
>NF7O_XENLA (Q91431) Nuclear factor 7, ovary (xnf7-O)
Length = 610
Score = 39.3 bits (90), Expect = 0.019
Identities = 19/61 (31%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Query: 20 CPISLELMRDPVTVCTGQTYDRSSIES-WVNTGNTTCPVTRAPLTEFTLIPNHTLRRLIQ 78
CP+ +EL +DPV V G + RS I+ W + CP + +T+ N L L +
Sbjct: 146 CPLCVELFKDPVMVACGHNFCRSCIDKVWEGQSSFACPECKESITDRKYTINRVLANLAK 205
Query: 79 E 79
+
Sbjct: 206 K 206
>TM30_MOUSE (P15533) Tripartite motif-containing protein 30 (Down
regulatory protein of interleukin 2 receptor)
Length = 496
Score = 38.9 bits (89), Expect = 0.024
Identities = 28/83 (33%), Positives = 41/83 (48%), Gaps = 11/83 (13%)
Query: 20 CPISLELMRDPVTVCTGQTYDRSSI----ESWVNT-GNTTCPVTRAPLTEFTLIPNHTLR 74
CPI LEL+++PV+ ++ R+ I ES NT G CPV R P L PN +
Sbjct: 15 CPICLELLKEPVSADCNHSFCRACITLNYESNRNTDGKGNCPVCRVPYPFGNLRPNLHVA 74
Query: 75 RLIQEWCVANRAFGVERIPTPKQ 97
+++ R G + IP +Q
Sbjct: 75 NIVE------RLKGFKSIPEEEQ 91
>TRM4_HUMAN (Q9C037) Tripartite motif-containing protein 4
Length = 500
Score = 37.4 bits (85), Expect = 0.071
Identities = 21/62 (33%), Positives = 30/62 (47%), Gaps = 2/62 (3%)
Query: 20 CPISLELMRDPVTVCTGQTYDRSSI-ESWVNTGNT-TCPVTRAPLTEFTLIPNHTLRRLI 77
CPI L+ +DPV++ G + R + +W G CP R P L PN L RL
Sbjct: 12 CPICLDYFQDPVSIECGHNFCRGCLHRNWAPGGGPFPCPECRHPSAPAALRPNWALARLT 71
Query: 78 QE 79
++
Sbjct: 72 EK 73
>UB4B_MOUSE (Q9ES00) Ubiquitin conjugation factor E4 B
(Ubiquitin-fusion degradation protein 2) (Ufd2a)
Length = 1173
Score = 37.0 bits (84), Expect = 0.093
Identities = 25/66 (37%), Positives = 32/66 (47%), Gaps = 1/66 (1%)
Query: 15 PYHYRCPISLELMRDPVTVCTGQTYDRSSIESWVNTGNTTCPVTRAPLTEFTLIPNHTLR 74
P +R P+ LM DPV + +G DRS I + + T P R LTE L P L+
Sbjct: 1100 PDEFRDPLMDTLMTDPVRLPSGTVMDRSIILRHL-LNSPTDPFNRQMLTESMLEPVPELK 1158
Query: 75 RLIQEW 80
IQ W
Sbjct: 1159 EQIQAW 1164
>UB4B_HUMAN (O95155) Ubiquitin conjugation factor E4 B
(Ubiquitin-fusion degradation protein 2) (Homozygously
deleted in neuroblastoma-1)
Length = 1302
Score = 37.0 bits (84), Expect = 0.093
Identities = 25/66 (37%), Positives = 32/66 (47%), Gaps = 1/66 (1%)
Query: 15 PYHYRCPISLELMRDPVTVCTGQTYDRSSIESWVNTGNTTCPVTRAPLTEFTLIPNHTLR 74
P +R P+ LM DPV + +G DRS I + + T P R LTE L P L+
Sbjct: 1229 PDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHL-LNSPTDPFNRQTLTESMLEPVPELK 1287
Query: 75 RLIQEW 80
IQ W
Sbjct: 1288 EQIQAW 1293
>UB4A_HUMAN (Q14139) Ubiquitin conjugation factor E4 A
Length = 1073
Score = 36.6 bits (83), Expect = 0.12
Identities = 24/61 (39%), Positives = 33/61 (53%), Gaps = 2/61 (3%)
Query: 21 PISLELMRDPVTVCTGQ-TYDRSSIESWVNTGNTTCPVTRAPLTEFTLIPNHTLRRLIQE 79
PI LM DPV + + + T DRS+I + + T P R+PLT + PN L+ IQ
Sbjct: 1002 PIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTD-PFNRSPLTMDQIRPNTELKEKIQR 1060
Query: 80 W 80
W
Sbjct: 1061 W 1061
>TM47_MOUSE (Q8C0E3) Tripartite motif-containing protein 47
Length = 641
Score = 36.2 bits (82), Expect = 0.16
Identities = 23/72 (31%), Positives = 35/72 (47%), Gaps = 11/72 (15%)
Query: 18 YRCPISLELMRDPVTVCTGQTY----------DRSSIESWVNTGNTTCPVTRAPLTE-FT 66
+ CPI LE +R+PVT+ G + RS+ + + G CP+ + P +
Sbjct: 7 FSCPICLEPLREPVTLPCGHNFCLACLGALWPHRSAGGTGGSGGPARCPLCQEPFPDGLQ 66
Query: 67 LIPNHTLRRLIQ 78
L NHTL L+Q
Sbjct: 67 LRKNHTLSELLQ 78
>TRM5_HUMAN (Q9C035) Tripartite motif-containing protein 5 (EC
6.3.2.-)
Length = 493
Score = 35.8 bits (81), Expect = 0.21
Identities = 16/73 (21%), Positives = 38/73 (51%), Gaps = 5/73 (6%)
Query: 12 IQIPYHYRCPISLELMRDPVTVCTGQTYDRSSI-----ESWVNTGNTTCPVTRAPLTEFT 66
+ + CPI LEL+ P+++ G ++ ++ + +S ++ G ++CPV R
Sbjct: 7 VNVKEEVTCPICLELLTQPLSLDCGHSFCQACLTANHKKSMLDKGESSCPVCRISYQPEN 66
Query: 67 LIPNHTLRRLIQE 79
+ PN + ++++
Sbjct: 67 IRPNRHVANIVEK 79
>TM26_MOUSE (Q99PN3) Tripartite motif-containing protein 26 (Zinc
finger protein 173)
Length = 545
Score = 35.8 bits (81), Expect = 0.21
Identities = 24/87 (27%), Positives = 38/87 (43%), Gaps = 13/87 (14%)
Query: 20 CPISLELMRDPVTVCTGQTYDRSSIESW--VNTGNTTCPVTRAPLTEFTLIPNHTLRRLI 77
C I L+ +RDPVT+ G + RS ++ CP+ + P + + P
Sbjct: 16 CSICLDYLRDPVTIDCGHVFCRSCTSDIRPISGNRPVCPLCKKPFKKENIRP-------- 67
Query: 78 QEWCVANRAFGVER--IPTPKQPAEPA 102
W +AN +ER + +QP E A
Sbjct: 68 -VWQLANLVENIERLKVDNGRQPGELA 93
>TM22_HUMAN (Q8IYM9) Tripartite motif-containing protein 22 (RING
finger protein 94) (50 kDa stimulated trans-acting
factor) (Staf-50)
Length = 498
Score = 35.8 bits (81), Expect = 0.21
Identities = 18/73 (24%), Positives = 37/73 (50%), Gaps = 6/73 (8%)
Query: 12 IQIPYHYRCPISLELMRDPVTVCTGQTYDRSSIES------WVNTGNTTCPVTRAPLTEF 65
+ I CPI LEL+ +P+++ G ++ ++ I + ++ G ++CPV +
Sbjct: 7 VDIEKEVTCPICLELLTEPLSLDCGHSFCQACITAKIKESVIISRGESSCPVCQTRFQPG 66
Query: 66 TLIPNHTLRRLIQ 78
L PN L +++
Sbjct: 67 NLRPNRHLANIVE 79
>VAC8_YEAST (P39968) Vacuolar protein 8
Length = 578
Score = 35.0 bits (79), Expect = 0.35
Identities = 37/119 (31%), Positives = 56/119 (46%), Gaps = 8/119 (6%)
Query: 259 ALFALCLVKQSRQRAVAAGAPAVLIDRLADFEKCDAERALATVELLCRVPAGCAEFAGHA 318
AL + +++R+ V AGA VL+ L+ + D + T L + A A
Sbjct: 190 ALLNMTHSEENRKELVNAGAVPVLVSLLSSTDP-DVQYYCTTA--LSNIAVDEANRKKLA 246
Query: 319 LTVPMLVKIILKISD----RATEYAAGALLSLCSESERLQMEAVAAGVLTQLLLLVQSD 373
T P LV ++ + D R A AL +L S++ Q+E V AG L L+ L+QSD
Sbjct: 247 QTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTS-YQLEIVRAGGLPHLVKLIQSD 304
>TM47_HUMAN (Q96LD4) Tripartite motif-containing protein 47 (RING
finger protein 100) (Gene overexpressed in astrocytoma
protein)
Length = 638
Score = 35.0 bits (79), Expect = 0.35
Identities = 22/72 (30%), Positives = 36/72 (49%), Gaps = 11/72 (15%)
Query: 18 YRCPISLELMRDPVTVCTGQTYDRSSIES-WVNTGNT---------TCPVTRAPLTE-FT 66
+ CPI LE +R+PVT+ G + + + + W + G + CP+ + P +
Sbjct: 7 FSCPICLEPLREPVTLPCGHNFCLACLGALWPHRGASGAGGPGGAARCPLCQEPFPDGLQ 66
Query: 67 LIPNHTLRRLIQ 78
L NHTL L+Q
Sbjct: 67 LRKNHTLSELLQ 78
>TM26_RAT (P62603) Tripartite motif-containing protein 26 (Zinc
finger protein 173)
Length = 542
Score = 35.0 bits (79), Expect = 0.35
Identities = 24/87 (27%), Positives = 38/87 (43%), Gaps = 13/87 (14%)
Query: 20 CPISLELMRDPVTVCTGQTYDRSSIESW--VNTGNTTCPVTRAPLTEFTLIPNHTLRRLI 77
C I L+ +RDPVT+ G + RS ++ CP+ + P + + P
Sbjct: 16 CSICLDYLRDPVTIDCGHVFCRSCTSDIRPISGNRPVCPLCKKPFKKENIRP-------- 67
Query: 78 QEWCVANRAFGVER--IPTPKQPAEPA 102
W +A+ +ER + KQP E A
Sbjct: 68 -VWQLASLVENIERLKVDNGKQPGELA 93
>RUVC_RHILO (Q98F72) Crossover junction endodeoxyribonuclease ruvC
(EC 3.1.22.4) (Holliday junction nuclease ruvC)
(Holliday juction resolvase ruvC)
Length = 169
Score = 34.7 bits (78), Expect = 0.46
Identities = 33/142 (23%), Positives = 65/142 (45%), Gaps = 14/142 (9%)
Query: 213 IEMVVAGT-HSADLRSQVSSVEGIHDGVVEILRSPISYPRALKVGVKALFALCLVKQSRQ 271
+ V +GT S D + + + +HDG+ E+L + + + A++ A+ +K +
Sbjct: 29 LRFVASGTVRSEDKAALATRLCQLHDGLAEVLHAAMPHEAAVEQTFVNKDAVATLKLGQA 88
Query: 272 RAVAAGAPAVLIDRLADFEKCDAERALATVELLCRVPAGCAEFAGHALTVPMLVKIILKI 331
R +A PA +A++ ++A+ V G + M+VK++L
Sbjct: 89 RGIAMLVPARAGLVVAEYAPNAVKKAVIGVG------------HGDKKQIHMMVKVLLPK 136
Query: 332 SDRATEYAAGAL-LSLCSESER 352
+ TE+AA AL +++C R
Sbjct: 137 ATFDTEHAADALAIAICHAHHR 158
>N192_YEAST (P47054) Nucleoporin NUP192 (Nuclear pore protein NUP192)
Length = 1683
Score = 34.7 bits (78), Expect = 0.46
Identities = 32/123 (26%), Positives = 61/123 (49%), Gaps = 5/123 (4%)
Query: 128 LRQLARDSDYNRSLIASHDVRRIVLRIFDNRSSDELSHESLALLVMFPLAESDCAAIAAD 187
LR LA+ S+ + +AS R +L IFD S DE + A + + +D +A
Sbjct: 942 LRILAKLSERSNGSVASLSKRNKLLTIFD--SVDESARIKDAFITQLESSITDAGVLALK 999
Query: 188 LDKIGYLSVLLSHQSLDVRVNSAALIEMVVAGTHS--ADLRSQVSSVEGIHDGVVEILRS 245
L+ + +L+ LS+ S + + S L+ V+ S +L + +SS + D ++ +L +
Sbjct: 1000 LELLDFLTSNLSNYSRTMTI-SHLLLGFQVSNVISLGPNLATFISSGTSLLDSLISVLEA 1058
Query: 246 PIS 248
++
Sbjct: 1059 SLN 1061
>A33_PLEWA (Q02084) Zinc-binding protein A33
Length = 624
Score = 34.7 bits (78), Expect = 0.46
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Query: 20 CPISLELMRDPVTVCTGQTYDRSSIE-SWVNTGNTTCPVTRAPLTEFTLIPNHTLRRLIQ 78
CP+ L ++PV + G + + I+ SW + +CP + LTE N L L++
Sbjct: 162 CPLCRSLFKEPVILECGHNFCKHCIDKSWESASAFSCPECKEVLTERKYTTNRVLANLVK 221
Query: 79 EWCV 82
+ V
Sbjct: 222 KAAV 225
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.135 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,623,777
Number of Sequences: 164201
Number of extensions: 1577153
Number of successful extensions: 3970
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 48
Number of HSP's that attempted gapping in prelim test: 3947
Number of HSP's gapped (non-prelim): 58
length of query: 414
length of database: 59,974,054
effective HSP length: 113
effective length of query: 301
effective length of database: 41,419,341
effective search space: 12467221641
effective search space used: 12467221641
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 67 (30.4 bits)
Lotus: description of TM0227.12