BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0227.11
         (425 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YK75_MYCTU (Q10683) Hypothetical protein Rv2075c/MT2135                42  0.002
VP22_ASFB7 (P23169) Protein P22 precursor (Protein K'177)              34  0.82
PLC1_YEAST (P32383) 1-phosphatidylinositol-4,5-bisphosphate phos...    33  1.1
RP3B_ARATH (Q39212) DNA-directed RNA polymerase II 36 kDa polype...    32  2.4
WUN_DROME (Q9V576) Putative phosphatidate phosphatase (EC 3.1.3....    32  4.0
PLC_BACCE (P14262) 1-phosphatidylinositol phosphodiesterase prec...    30  9.0
ITAX_HUMAN (P20702) Integrin alpha-X precursor (Leukocyte adhesi...    30  9.0
ATK1_ARATH (Q07970) Kinesin 1 (Kinesin-like protein A)                 30  9.0

>YK75_MYCTU (Q10683) Hypothetical protein Rv2075c/MT2135
          Length = 487

 Score = 42.4 bits (98), Expect = 0.002
 Identities = 37/126 (29%), Positives = 59/126 (46%), Gaps = 14/126 (11%)

Query: 79  LPFNRYSWLTTHNSFALLGQKSMTGSVILSPTNQQDTITDQLNNGVRGLMLDLYDFEN-- 136
           +P     WL THNSF  L   S + +V  + +NQQ ++  QL+  VR L LDL+      
Sbjct: 112 VPLRETQWLGTHNSFNSL---SDSFTVSHADSNQQLSLAQQLDIDVRALELDLHYLPRLE 168

Query: 137 -----DVWLCHSFGGQCYNY-TAFQPAI-NVLKEIQVFLEA--NPSEIVTIIIEDYVTSP 187
                 V +CH  G +  N     +P +  VL +I  +L A  +  E++ + +ED + + 
Sbjct: 169 GHGAPGVTVCHGLGPKNANLGCTVEPLLATVLPQIANWLNAPGHTEEVILLYLEDQLKNA 228

Query: 188 KGLTKV 193
                V
Sbjct: 229 SAYESV 234


>VP22_ASFB7 (P23169) Protein P22 precursor (Protein K'177)
          Length = 177

 Score = 33.9 bits (76), Expect = 0.82
 Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 5/52 (9%)

Query: 7  TLAIATTLFTILVLLDSSLALKFKPLQQEGQICVANKNCNSGLHC--ETCVT 56
          TL IA  +  I++L+   + L +K  Q   ++C  +K+C SG HC   TC T
Sbjct: 10 TLLIALIILVIIILV---VFLYYKKQQPPKKVCKVDKDCGSGEHCVRGTCST 58


>PLC1_YEAST (P32383) 1-phosphatidylinositol-4,5-bisphosphate
           phosphodiesterase 1 (EC 3.1.4.11) (Phosphoinositide
           phospholipase C) (PLC-1) (Phospholipase C-1)
          Length = 869

 Score = 33.5 bits (75), Expect = 1.1
 Identities = 38/168 (22%), Positives = 72/168 (42%), Gaps = 24/168 (14%)

Query: 80  PFNRYSWLTTHNSFALLGQKSMTGSVILSPTNQQDTITDQLNNGVRGLMLDLYDFENDVW 139
           P N Y   ++HN++ L  Q + T SV        +     L  G R + +D++D EN   
Sbjct: 385 PLNHYFIASSHNTYLLGKQIAETPSV--------EGYIQVLQQGCRCVEIDIWDGENGPV 436

Query: 140 LCHSFGGQCYNYTAFQPAINVLKEIQ--VFLEANPSEIVTIIIEDYVTSPKGLTKVFDAA 197
           +CH F       T+  P   V++ I+   F+ +    I+++ I     + K  + +    
Sbjct: 437 VCHGF------LTSAIPLKTVIRVIKKYAFITSPYPLIISLEINCNKDNQKLASLIMREV 490

Query: 198 GLRKYWFPVSRMPKNGGDWPKVDDMVQKNQRLVVFTSKASKEASEGIA 245
              + +F  +R  K     P   ++  K    ++  SK + EA+ G++
Sbjct: 491 LAEQLYFVGTRTDK----LPSPRELKHK----ILLKSKKTSEATRGLS 530


>RP3B_ARATH (Q39212) DNA-directed RNA polymerase II 36 kDa
           polypeptide B (EC 2.7.7.6) (RNA polymerase II subunit 3)
          Length = 319

 Score = 32.3 bits (72), Expect = 2.4
 Identities = 24/83 (28%), Positives = 37/83 (43%), Gaps = 8/83 (9%)

Query: 1   MMMQKPTLAIATTLFTI--LVLLDSSLA--LKFKPLQQEG----QICVANKNCNSGLHCE 52
           M+ + PT+AI      +   VL D  +A  L   PL  E     + C   ++CN   HCE
Sbjct: 41  MISEVPTMAIHLVKIEVNSSVLNDEFIAQRLSLIPLTSERAMSMRFCQDCEDCNGDEHCE 100

Query: 53  TCVTNGNVRPRCTRTQPINPTSK 75
            C     +  +C   Q ++ TS+
Sbjct: 101 FCSVEFPLSAKCVTDQTLDVTSR 123


>WUN_DROME (Q9V576) Putative phosphatidate phosphatase (EC 3.1.3.4)
           (Phosphatidic acid phosphatase type 2) (Wunen protein)
           (Germ cell guidance factor)
          Length = 379

 Score = 31.6 bits (70), Expect = 4.0
 Identities = 24/82 (29%), Positives = 41/82 (49%), Gaps = 9/82 (10%)

Query: 268 NRAESPSMNTTSRSLVLVNFFRDLPDVAQSCKDN-----SAPLLSMVST--CNQAAGKRW 320
           N+A+  + N TSR  V +N+  +LPD    C           +LS ++T     + G+  
Sbjct: 161 NKAKQDNGNATSRRYVFMNY--ELPDWMIECYKKIGIYAFGAVLSQLTTDIAKYSIGRLR 218

Query: 321 PNFIAVDFYKRSDGGGAPEAVD 342
           P+FIAV   + +DG    +A++
Sbjct: 219 PHFIAVCQPQMADGSTCDDAIN 240


>PLC_BACCE (P14262) 1-phosphatidylinositol phosphodiesterase
           precursor (EC 4.6.1.13) (Phosphatidylinositol
           diacylglycerol-lyase) (Phosphatidylinositol-specific
           phospholipase C) (PI-PLC)
          Length = 329

 Score = 30.4 bits (67), Expect = 9.0
 Identities = 23/80 (28%), Positives = 36/80 (44%), Gaps = 10/80 (12%)

Query: 124 VRGLMLDLYDFENDVWLCHSFGGQCYNYTAFQPAINVLKEIQVFLEANPSEIVTIIIEDY 183
           +RG + D    +N + L H   G  Y Y      IN  K+   FL+ NPSE + + ++  
Sbjct: 99  IRGRLTD----DNTIVLHH---GPLYLYVTLHEFINEAKQ---FLKDNPSETIIMSLKKE 148

Query: 184 VTSPKGLTKVFDAAGLRKYW 203
               KG    F +   +KY+
Sbjct: 149 YEDMKGAEDSFSSTFEKKYF 168


>ITAX_HUMAN (P20702) Integrin alpha-X precursor (Leukocyte adhesion
           glycoprotein p150,95 alpha chain) (Leukocyte adhesion
           receptor p150,95) (CD11c) (Leu M5)
          Length = 1163

 Score = 30.4 bits (67), Expect = 9.0
 Identities = 23/99 (23%), Positives = 42/99 (42%), Gaps = 12/99 (12%)

Query: 142 HSFGGQCYNYTAFQPAINVLKEIQVFLEANPSEIVTIIIE--------DYVTSPKGLTKV 193
           H   G  Y  TA Q  ++ L         + ++I+ +I +        DY    K +  +
Sbjct: 220 HQLQGFTYTATAIQNVVHRLFHASYGARRDATKILIVITDGKKEGDSLDY----KDVIPM 275

Query: 194 FDAAGLRKYWFPVSRMPKNGGDWPKVDDMVQKNQRLVVF 232
            DAAG+ +Y   V    +N   W +++D+  K  +  +F
Sbjct: 276 ADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIF 314


>ATK1_ARATH (Q07970) Kinesin 1 (Kinesin-like protein A)
          Length = 793

 Score = 30.4 bits (67), Expect = 9.0
 Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 1/61 (1%)

Query: 222 MVQKNQRLVVFTSKASKEASEGIAYEWRYLVENQYGNGGMKAGSCPNRAESPSMNTTSRS 281
           M  +NQ     +  A KE   GI+++ R  VE Q G G  +A S  N+ +  +MN+   S
Sbjct: 1   MASRNQNRPPRSPNAKKEGLGGISFDKRRKVETQGGTGRRQAFSAVNK-QDVTMNSDVGS 59

Query: 282 L 282
           +
Sbjct: 60  I 60


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.321    0.136    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,293,062
Number of Sequences: 164201
Number of extensions: 2128312
Number of successful extensions: 4222
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 4220
Number of HSP's gapped (non-prelim): 8
length of query: 425
length of database: 59,974,054
effective HSP length: 113
effective length of query: 312
effective length of database: 41,419,341
effective search space: 12922834392
effective search space used: 12922834392
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 67 (30.4 bits)