BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0223.4
(336 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YXCE_BACSU (P46335) Hypothetical protein yxcE 37 0.054
IF2P_SCHPO (Q10251) Eukaryotic translation initiation factor 5B ... 36 0.12
YF06_MYCPN (P75280) Hypothetical lipoprotein MPN506 precursor (P... 36 0.16
DYHC_DICDI (P34036) Dynein heavy chain, cytosolic (DYHC) 34 0.45
KF3A_HUMAN (Q9Y496) Kinesin-like protein KIF3A (Microtubule plus... 33 1.0
GAG_MPMV (P07567) Gag polyprotein [Contains: Core protein p10; C... 33 1.0
C190_DROME (Q9VJE5) Restin homolog (Cytoplasmic linker protein 1... 33 1.0
ASX_DROME (Q9V727) Polycomb protein Asx (Additional sex combs) 33 1.0
MTR4_YEAST (P47047) ATP-dependent RNA helicase DOB1 (mRNA transp... 33 1.3
Y8B7_BACTN (Q89ZG0) Hypothetical UPF0144 protein BT4417 32 1.7
YKZ6_CAEEL (P34333) Hypothetical protein C14B9.6 in chromosome III 32 2.3
TRHY_SHEEP (P22793) Trichohyalin 32 2.9
RPF1_YEAST (P38805) Ribosome biogenesis protein RPF1 32 2.9
MED4_HUMAN (Q9NPJ6) Mediator of RNA polymerase II transcription ... 32 2.9
YP52_BORBU (Q44845) Putative outer membrane protein BBA52 31 3.8
SR72_CANFA (P33731) Signal recognition particle 72 kDa protein (... 31 3.8
RA50_PYRFU (P58301) DNA double-strand break repair rad50 ATPase 31 3.8
KINH_LOLPE (P21613) Kinesin heavy chain 31 3.8
IRS1_RAT (P35570) Insulin receptor substrate-1 (pp185) 31 3.8
IF2P_YEAST (P39730) Eukaryotic translation initiation factor 5B ... 31 3.8
>YXCE_BACSU (P46335) Hypothetical protein yxcE
Length = 216
Score = 37.4 bits (85), Expect = 0.054
Identities = 20/80 (25%), Positives = 38/80 (47%)
Query: 184 TNSRGQKKSGAGADGTSTDPSASIDCDEYEATPPTTRPKGKKAEKRKAKTTDTASSTLSF 243
T S + SG + S+ S+S Y+++P ++ G + +K+KT+D +SS++S
Sbjct: 23 TISHAKSFSGRSSSSYSSRSSSSSYSGSYKSSPKSSYSSGSSSSSKKSKTSDDSSSSISL 82
Query: 244 VPHPDVLAMGKAKMEMMANF 263
P A + + F
Sbjct: 83 KKKPSEKASSSSSKKSSGTF 102
>IF2P_SCHPO (Q10251) Eukaryotic translation initiation factor 5B
(eIF-5B) (Translation initiation factor IF-2)
Length = 1079
Score = 36.2 bits (82), Expect = 0.12
Identities = 33/133 (24%), Positives = 55/133 (40%), Gaps = 17/133 (12%)
Query: 172 KDEPKWKGQSMKTNSRGQKKSGAGADGTSTDPSASIDCDEYEATPPTTRPKGKKAEKRKA 231
K+ K + + ++ + KK G+ + T S + D + KGK+A K
Sbjct: 183 KEREKKEREKLRKKQQQAKKKGSTGEDTLASSEVSSEVDISTPAENDSSAKGKQAAGSKR 242
Query: 232 KTTDTASSTLSFVPHPDVLAMGKAKMEMMANFRE---IRNRELDLQQADQQLKQSELQLR 288
K P+V A+ K E A E IR E + + +++L + E + R
Sbjct: 243 K-------------GPNVTALQKMLEEKRAREEEEQRIREEEARIAEEEKRLAEVE-EAR 288
Query: 289 QEELKFKKAENFR 301
+EE + KK E R
Sbjct: 289 KEEARLKKKEKER 301
>YF06_MYCPN (P75280) Hypothetical lipoprotein MPN506 precursor
(P02_orf793)
Length = 793
Score = 35.8 bits (81), Expect = 0.16
Identities = 33/136 (24%), Positives = 58/136 (42%), Gaps = 23/136 (16%)
Query: 180 QSMKTNSRGQKKSGAGADGTSTDPSASIDCDEYEATPPTTRPKGKKAEKRKAKTTDTASS 239
Q M ++S G+ SG T T+ S+ ++ + + GK EK K K D S+
Sbjct: 183 QKMTSSSEGKNSSGTLTVATDTETSSL-----WKKIEDSAKANGKSDEKGKGKKKDNKSA 237
Query: 240 TLSFVPHPDVLAMGKAKMEMMANFREIRNRELDLQQADQQLKQSELQLRQEELKFK---- 295
T S V + +E + D + +D Q+K+S + ++ + K
Sbjct: 238 TFSLV--------------QLKQTQEKTDDSQDTKNSDDQVKKSWGEYQEVDGVLKNFEF 283
Query: 296 KAENFRAYMDILNKNT 311
KA F + D+L+ +T
Sbjct: 284 KASIFENWHDLLDFST 299
>DYHC_DICDI (P34036) Dynein heavy chain, cytosolic (DYHC)
Length = 4725
Score = 34.3 bits (77), Expect = 0.45
Identities = 18/45 (40%), Positives = 28/45 (62%), Gaps = 2/45 (4%)
Query: 267 RNRELDL--QQADQQLKQSELQLRQEELKFKKAENFRAYMDILNK 309
+NRELD+ +QA+Q+LKQ + E+K K A + +D+ NK
Sbjct: 3304 KNRELDVKNEQANQKLKQMVQDQQAAEIKQKDARELQVQLDVRNK 3348
>KF3A_HUMAN (Q9Y496) Kinesin-like protein KIF3A (Microtubule plus
end-directed kinesin motor 3A)
Length = 702
Score = 33.1 bits (74), Expect = 1.0
Identities = 26/104 (25%), Positives = 52/104 (50%), Gaps = 13/104 (12%)
Query: 189 QKKSGAGADGTSTDPSASIDCDEYEATPPTTRPKGKKAEKRKAKTTDTASSTLSFVPHPD 248
+KK G + + +D S S + D+ E G+ EKRK + T +S PD
Sbjct: 371 KKKLEEGEEISGSDISGSEEDDDEEGEV------GEDGEKRKKRRDQTGKKKVS----PD 420
Query: 249 VLAMGKAKMEMMANFREIRNRELDLQQADQQLKQSELQLRQEEL 292
+ +AK++ R+ +LD+++ ++ ++EL+ R+++L
Sbjct: 421 KMIEMQAKID---EERKALETKLDMEEEERNKARAELEKREKDL 461
>GAG_MPMV (P07567) Gag polyprotein [Contains: Core protein p10; Core
phosphoprotein pp24; Core protein p12; Core protein p27;
Core protein p14; Core protein p4]
Length = 657
Score = 33.1 bits (74), Expect = 1.0
Identities = 30/96 (31%), Positives = 44/96 (45%), Gaps = 17/96 (17%)
Query: 18 TGSKVSDTQCEATPD------DTQHEGLDDIDLEDEDQSSGKKRTRWRVKDDLLLVQSWL 71
+ S+ T+ PD D+ EG L+D+ SS KK R+ V LL Q+
Sbjct: 112 SNSQTDLTKTSQNPDLDLISLDSDDEGAKSSSLQDKGLSSTKKPKRFPV---LLTAQT-- 166
Query: 72 NISKDPTVGTDQTAAKF-WDRIRDQFDEYRDFDTPP 106
SKDP D ++ WD + D+ +Y + D PP
Sbjct: 167 --SKDPE---DPNPSEVDWDGLEDEAAKYHNPDWPP 197
>C190_DROME (Q9VJE5) Restin homolog (Cytoplasmic linker protein 190)
(Microtubule binding protein 190) (d-CLIP-190)
Length = 1690
Score = 33.1 bits (74), Expect = 1.0
Identities = 32/126 (25%), Positives = 55/126 (43%), Gaps = 13/126 (10%)
Query: 210 DEYEATPPTTRPKGKKAEKRKAKTTDTASSTLSFVPHPDVLAMGKAKMEMMANFREIRNR 269
DE A + K E + K S S +P PD+ + A E +A +E
Sbjct: 441 DELNAQSQVYKEKIHDLESKITKLVSATPSLQSILP-PDLPSDDGALQEEIAKLQE---- 495
Query: 270 ELDLQQADQQLKQSELQLRQEELKFKKAENFRAYMDILNKNTSGMNDEELRTHNALRAFA 329
++ +QQ + + + +E QL +E+ R + LN+ + + E + AL F+
Sbjct: 496 KMTIQQKEVESRIAE-QLEEEQ-------RLRENVKYLNEQIATLQSELVSKDEALEKFS 547
Query: 330 LSELGM 335
LSE G+
Sbjct: 548 LSECGI 553
>ASX_DROME (Q9V727) Polycomb protein Asx (Additional sex combs)
Length = 1669
Score = 33.1 bits (74), Expect = 1.0
Identities = 27/108 (25%), Positives = 45/108 (41%), Gaps = 12/108 (11%)
Query: 117 GKLSKDIQFFTGCYN-----KVTTPWKSGHSEKDIMAEAHALFQVDHKKDFTHENVWRMV 171
G S+ I+ C N + W+ SE + E + + +++ + W++
Sbjct: 268 GSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNKLDPWKLK 327
Query: 172 KDEPKWKGQSMKTNSRGQKKSGAGADGTSTDPSASIDCDEYEATPPTT 219
EP W + NSRG+ K +D + SASI + E PP T
Sbjct: 328 HFEPFWG----EKNSRGKDKDKLESDCKNQKLSASI---KSEPKPPAT 368
>MTR4_YEAST (P47047) ATP-dependent RNA helicase DOB1 (mRNA
transport regulator MTR4)
Length = 1073
Score = 32.7 bits (73), Expect = 1.3
Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
Query: 14 PTGSTGSKVSDTQCEATPDDTQHE--GLDDIDLEDEDQSSGKKRTR 57
PT S G K +DT TPD TQ + GL++ E E+ +S K+ +
Sbjct: 18 PTDSNGEKNADTNVGDTPDHTQDKKHGLEEEKEEHEENNSENKKIK 63
>Y8B7_BACTN (Q89ZG0) Hypothetical UPF0144 protein BT4417
Length = 511
Score = 32.3 bits (72), Expect = 1.7
Identities = 21/54 (38%), Positives = 32/54 (58%), Gaps = 8/54 (14%)
Query: 264 REIRNRELDLQQADQQLKQSELQL--RQEELKFKK------AENFRAYMDILNK 309
+E+ R +QQA+ +LKQ E+ L RQEE++ KK EN A + I++K
Sbjct: 66 KEVALRNQKIQQAENKLKQREMVLSQRQEEIQRKKLEAEAVKENLEAQLVIVDK 119
>YKZ6_CAEEL (P34333) Hypothetical protein C14B9.6 in chromosome III
Length = 1780
Score = 32.0 bits (71), Expect = 2.3
Identities = 25/96 (26%), Positives = 43/96 (44%), Gaps = 5/96 (5%)
Query: 18 TGSKVSDTQCEATPDDTQHEGLDDIDLEDEDQSSGKKRTRWRVKDDLLLVQSWLNISKDP 77
TG V+ Q P + E +D++++ED D+ + + K S ++ D
Sbjct: 550 TGGAVTRAQAVPKPVEDLGEEIDEMEIEDNDEDASRGSRGKDSKAPSDRDGSPADMEGDS 609
Query: 78 TVGTDQTAAKFWDRIRDQFDE-----YRDFDTPPRT 108
G DQ A + D+ +D +E RD D+P +T
Sbjct: 610 PEGQDQDADQDQDQDQDVDEEEEEVIVRDIDSPVKT 645
>TRHY_SHEEP (P22793) Trichohyalin
Length = 1549
Score = 31.6 bits (70), Expect = 2.9
Identities = 17/53 (32%), Positives = 34/53 (64%), Gaps = 1/53 (1%)
Query: 254 KAKMEMMANFREIRNRELDLQQADQQLKQSELQLRQEEL-KFKKAENFRAYMD 305
K + E RE + RE++LQ+ +++L++ E QL++EE K ++ E + Y++
Sbjct: 402 KLQREKRRQERERQYREVELQREEERLQREEEQLQREEREKRRRQEREKQYLE 454
>RPF1_YEAST (P38805) Ribosome biogenesis protein RPF1
Length = 295
Score = 31.6 bits (70), Expect = 2.9
Identities = 19/73 (26%), Positives = 38/73 (52%), Gaps = 4/73 (5%)
Query: 256 KMEMMANFREIRNRELDLQQADQQLKQSEL-QLRQEELK---FKKAENFRAYMDILNKNT 311
+ E+ A+ + +N+E + + ++ E +LR++ LK + EN R Y + +NK
Sbjct: 15 RQEIFADIKHEKNKERHTMRRKRAKEERENPELREQRLKENVTQTIENTRVYDETINKEV 74
Query: 312 SGMNDEELRTHNA 324
G D+ +R N+
Sbjct: 75 EGDEDDLMRYFNS 87
>MED4_HUMAN (Q9NPJ6) Mediator of RNA polymerase II transcription
subunit 4 (Vitamin D3 receptor-interacting protein
complex 36 kDa component) (DRIP36) (Activator-recruited
cofactor 36 kDa component) (ARC36) (TRAP/SMCC/PC2
subunit p36 subunit) (HSPC126)
Length = 270
Score = 31.6 bits (70), Expect = 2.9
Identities = 20/56 (35%), Positives = 28/56 (49%), Gaps = 4/56 (7%)
Query: 250 LAMGKAKMEMMANFREIRNRELDLQQADQQLKQSELQLR----QEELKFKKAENFR 301
L GK EM +E+ R+ D+QQ +QLK++E L Q + K K E R
Sbjct: 84 LNQGKIHHEMQVLEKEVEKRDSDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKAR 139
>YP52_BORBU (Q44845) Putative outer membrane protein BBA52
Length = 281
Score = 31.2 bits (69), Expect = 3.8
Identities = 21/65 (32%), Positives = 32/65 (48%), Gaps = 13/65 (20%)
Query: 32 DDTQHEGLDDIDLEDEDQSSGKKRTRWRVKDDLLLVQSWLNISKDPTVGTDQTAAKFWDR 91
D+ + E DD +LE ED G++ +DD S+D T DQ+ +F+D
Sbjct: 229 DELEREYQDD-ELESEDPDDGERE----YQDD--------RESRDDTFNEDQSEDEFFDS 275
Query: 92 IRDQF 96
+ DQF
Sbjct: 276 LEDQF 280
>SR72_CANFA (P33731) Signal recognition particle 72 kDa protein
(SRP72)
Length = 670
Score = 31.2 bits (69), Expect = 3.8
Identities = 18/73 (24%), Positives = 34/73 (45%), Gaps = 7/73 (9%)
Query: 169 RMVKDEPKWKGQSMKTNSRGQKKS------GAGADGTSTDPSASIDCDEYEATPPTTRPK 222
++ D +W ++ RG+KK G G G + S+ +D + ++PPT+ P+
Sbjct: 568 KVTPDPERWLPMRERSYYRGRKKGKKKDQIGKGTQGATAGASSELDASKTVSSPPTS-PR 626
Query: 223 GKKAEKRKAKTTD 235
A A T++
Sbjct: 627 PGSAATASASTSN 639
>RA50_PYRFU (P58301) DNA double-strand break repair rad50 ATPase
Length = 882
Score = 31.2 bits (69), Expect = 3.8
Identities = 14/47 (29%), Positives = 28/47 (58%)
Query: 252 MGKAKMEMMANFREIRNRELDLQQADQQLKQSELQLRQEELKFKKAE 298
+ KAK + +F E+ E D+++ +++L Q + + +EE K K+ E
Sbjct: 624 IAKAKETLEMSFEELAEVEADIERIEKKLSQLKQKYNEEEYKKKREE 670
>KINH_LOLPE (P21613) Kinesin heavy chain
Length = 967
Score = 31.2 bits (69), Expect = 3.8
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 5/73 (6%)
Query: 262 NFREIRNRELDLQQADQQLKQSELQLRQEELKFKKAENFRAYMDILNKNTSGMNDE--EL 319
NF++I E DL ++Q E ++ + K +EN + +L N +N+E +L
Sbjct: 615 NFKKIETHEKDLSNCKLLIQQHEAKMASLQEAIKDSENKKR---MLEDNVDSLNEEYAKL 671
Query: 320 RTHNALRAFALSE 332
+ + ALSE
Sbjct: 672 KAQEQMHLAALSE 684
>IRS1_RAT (P35570) Insulin receptor substrate-1 (pp185)
Length = 1235
Score = 31.2 bits (69), Expect = 3.8
Identities = 28/105 (26%), Positives = 44/105 (41%), Gaps = 10/105 (9%)
Query: 184 TNSRGQKKSGAGADGTSTDPSASIDCDEYEATPP---TTRPKGKKAEKRKAKTTDTASST 240
++S G+ + A A+ +S+ S+ Y P T P + + DTA+ T
Sbjct: 787 SSSSGRLRYTATAEDSSSSTSSDSLGGGYCGARPESSVTHPHHHALQPHLPRKVDTAAQT 846
Query: 241 LSFVPHPDVLAMGKAKMEMMANFREIRNRELDLQQADQQLKQSEL 285
S + P L++G K + RE QQ QQ +QS L
Sbjct: 847 NSRLARPTRLSLGDPKASTLPRVRE-------QQQQQQQQQQSSL 884
>IF2P_YEAST (P39730) Eukaryotic translation initiation factor 5B
(eIF-5B) (Translation initiation factor IF-2)
Length = 1002
Score = 31.2 bits (69), Expect = 3.8
Identities = 32/161 (19%), Positives = 61/161 (37%), Gaps = 12/161 (7%)
Query: 159 KKDFTHENVWRMVKDEPKWKGQSMKTNSRGQKKSGAGADGTSTDPSASID-CDEYEATPP 217
K +N W +E + + + +S AGAD TS + SAS + + E
Sbjct: 4 KSKKNQQNYWDEEFEEDAAQNEEISATPTPNPESSAGADDTSREASASAEGAEAIEGDFM 63
Query: 218 TTRPKGKKAEKRKAKTTDTASSTLSFVPHPDVLAMGKAKMEMMANFREIRNRELDLQQAD 277
+T + KK +++K D + K+K E +E ++ Q A
Sbjct: 64 STLKQSKKKQEKKV-----------IEEKKDGKPILKSKKEKEKEKKEKEKQKKKEQAAR 112
Query: 278 QQLKQSELQLRQEELKFKKAENFRAYMDILNKNTSGMNDEE 318
++ +Q + + +EL + E A K+ + +
Sbjct: 113 KKAQQQAQKEKNKELNKQNVEKAAAEKAAAEKSQKSKGESD 153
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.313 0.129 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,064,102
Number of Sequences: 164201
Number of extensions: 1838803
Number of successful extensions: 5316
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 42
Number of HSP's that attempted gapping in prelim test: 5281
Number of HSP's gapped (non-prelim): 75
length of query: 336
length of database: 59,974,054
effective HSP length: 111
effective length of query: 225
effective length of database: 41,747,743
effective search space: 9393242175
effective search space used: 9393242175
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0223.4