BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0212.10
(115 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NCO6_MOUSE (Q9JL19) Nuclear receptor coactivator 6 (Amplified in... 30 0.98
Y309_MYCPN (P75334) Hypothetical lipoprotein MG309 homolog precu... 30 1.3
MRP2_RAT (Q63120) Canalicular multispecific organic anion transp... 29 1.7
CGB2_RANJA (O93229) G2/mitotic-specific cyclin B2 29 1.7
AG43_ECOLI (P39180) Antigen 43 precursor (AG43) (Fluffing protein) 29 2.2
YE30_HELPJ (Q9ZJI6) Hypothetical UPF0036 protein JHP1323 28 2.9
TOP2_TRYCR (P30190) DNA topoisomerase II (EC 5.99.1.3) 28 2.9
K682_HUMAN (Q9Y4C8) Probable RNA-binding protein KIAA0682 28 2.9
ATS3_HUMAN (O15072) ADAMTS-3 precursor (EC 3.4.24.-) (A disinteg... 28 2.9
ZBP1_RAT (Q8VDA5) Z-DNA binding protein 1 (Tumor stroma and acti... 28 3.7
YNO3_YEAST (P53908) Hypothetical 15.3 kDa protein in MFA2-MEP2 i... 28 3.7
T2M3_METJA (Q58017) Type II restriction enzyme MjaIII (EC 3.1.21... 28 3.7
KF4A_XENLA (Q91784) Chromosome-associated kinesin KLP1 (Chromoki... 28 3.7
SMY2_YEAST (P32909) SMY2 protein 28 4.9
VIT2_CHICK (P02845) Vitellogenin II precursor (Major vitellogeni... 27 6.4
Y285_MYCPN (P75380) Hypothetical protein MG285 homolog (F11_orf346) 27 8.3
SPEN_DROME (Q8SX83) Split ends protein 27 8.3
OPPB_BACSU (P24138) Oligopeptide transport system permease prote... 27 8.3
MRP2_MOUSE (Q8VI47) Canalicular multispecific organic anion tran... 27 8.3
KPB1_RABIT (P18688) Phosphorylase B kinase alpha regulatory chai... 27 8.3
>NCO6_MOUSE (Q9JL19) Nuclear receptor coactivator 6 (Amplified in
breast cancer-3 protein) (Cancer-amplified
transcriptional coactivator ASC-2) (Activating signal
cointegrator-2) (ASC-2) (Peroxisome
proliferator-activated receptor-interacting protein) (PP
Length = 2067
Score = 30.0 bits (66), Expect = 0.98
Identities = 14/45 (31%), Positives = 27/45 (59%)
Query: 3 RSFTTRRYERLGKETASSALLQEGFKRSTSLPSRASSSARKMAHA 47
++ T+RR R + T +S ++ G ++ +S P+ ASSS + + A
Sbjct: 2014 KTLTSRRNSRTEEPTMASESVENGHRKRSSRPASASSSTKDITGA 2058
>Y309_MYCPN (P75334) Hypothetical lipoprotein MG309 homolog precursor
(H08_orf1325)
Length = 1325
Score = 29.6 bits (65), Expect = 1.3
Identities = 23/97 (23%), Positives = 42/97 (42%), Gaps = 7/97 (7%)
Query: 15 KETASSALLQEGFKRSTSLPSRASSSARKMAHATFGNINLQRNPTKKANNSSKSTHPLFS 74
++T+ ++ + FK+ST R +SAR AT + +++ N + K +FS
Sbjct: 1156 QKTSLTSFVDSNFKQSTQSAQRGDTSARSARSAT---VQIKKTQEDNQNTNYKD---VFS 1209
Query: 75 FLDFRRKKKTTARPEFTRYLEYLKEGGMWDLNSNKPV 111
D +T Y ++L +GG + K V
Sbjct: 1210 RFDGYIGDNKVEEKNYTSY-QFLSDGGKYHATFVKQV 1245
>MRP2_RAT (Q63120) Canalicular multispecific organic anion
transporter 1 (ATP-binding cassette, sub-family C,
member 2) (Multidrug resistance-associated protein 2)
(Canalicular multidrug resistance protein)
Length = 1541
Score = 29.3 bits (64), Expect = 1.7
Identities = 23/98 (23%), Positives = 42/98 (42%), Gaps = 13/98 (13%)
Query: 11 ERLGKETASSALLQEGFKRSTSLPSRASSSARKMA---HATFGNINLQRNPTKKANNSSK 67
E + ++ AS A+ +E R T S SSS R + N+N+ + K+
Sbjct: 888 EEIPEDAASLAMRRENSLRRTLSRSSRSSSRRGKSLKNSLKIKNVNVLKEKEKEVEGQKL 947
Query: 68 STHPLFSFLDFRRKKKTTARPEFTRYLEYLKEGGMWDL 105
+++ T + +F+ YL+YL+ G W +
Sbjct: 948 ----------IKKEFVETGKVKFSIYLKYLQAVGWWSI 975
>CGB2_RANJA (O93229) G2/mitotic-specific cyclin B2
Length = 392
Score = 29.3 bits (64), Expect = 1.7
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 3/76 (3%)
Query: 17 TASSALLQEGFKRSTSLPSRASSSARKMAHATFGN---INLQRNPTKKANNSSKSTHPLF 73
T +A+ +E ++ S+A + R+ A GN + + P K +N +SK+T P+
Sbjct: 3 TRRAAIAREVDNAVGAMRSKAQLNGRRAALGEIGNKVTVRAVKQPAKNSNTTSKTTRPVA 62
Query: 74 SFLDFRRKKKTTARPE 89
+ K K E
Sbjct: 63 KVSNVSVKPKAVTVTE 78
>AG43_ECOLI (P39180) Antigen 43 precursor (AG43) (Fluffing protein)
Length = 1039
Score = 28.9 bits (63), Expect = 2.2
Identities = 16/50 (32%), Positives = 26/50 (52%)
Query: 1 MLRSFTTRRYERLGKETASSALLQEGFKRSTSLPSRASSSARKMAHATFG 50
++R+F++R R+G TA S + + TSL +A AR + T G
Sbjct: 963 VIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLG 1012
>YE30_HELPJ (Q9ZJI6) Hypothetical UPF0036 protein JHP1323
Length = 692
Score = 28.5 bits (62), Expect = 2.9
Identities = 19/79 (24%), Positives = 34/79 (42%), Gaps = 4/79 (5%)
Query: 27 FKRSTSLPSRASSSARKMA----HATFGNINLQRNPTKKANNSSKSTHPLFSFLDFRRKK 82
F+R T+ R +A+K A H T + + P KK NN K H ++ +++
Sbjct: 29 FERFTNRKKRFRENAQKNAESSNHETLSHHKKEHRPNKKPNNHHKPKHASQKTRNYAKEE 88
Query: 83 KTTARPEFTRYLEYLKEGG 101
+ E + ++ E G
Sbjct: 89 LDNNKVEGVTEILHVNERG 107
>TOP2_TRYCR (P30190) DNA topoisomerase II (EC 5.99.1.3)
Length = 1232
Score = 28.5 bits (62), Expect = 2.9
Identities = 14/42 (33%), Positives = 20/42 (47%)
Query: 50 GNINLQRNPTKKANNSSKSTHPLFSFLDFRRKKKTTARPEFT 91
G I++ P K KSTH FS +F + +KT +T
Sbjct: 544 GFISIFSTPIVKVRLRDKSTHSFFSLKEFHKWQKTHGNVSYT 585
>K682_HUMAN (Q9Y4C8) Probable RNA-binding protein KIAA0682
Length = 960
Score = 28.5 bits (62), Expect = 2.9
Identities = 20/68 (29%), Positives = 32/68 (46%), Gaps = 10/68 (14%)
Query: 15 KETASSALLQEGFKRSTSLPSRASSSARKMAHATFGNINLQRNPTKKANNSSKSTHPLFS 74
K+T S L++ ++P +A S + +TFG + R P K + TH F
Sbjct: 828 KQTTSKILVR-------NIPFQAHSREIRELFSTFGELKTVRLPKKM---TGTGTHRGFG 877
Query: 75 FLDFRRKK 82
F+DF K+
Sbjct: 878 FVDFLTKQ 885
>ATS3_HUMAN (O15072) ADAMTS-3 precursor (EC 3.4.24.-) (A disintegrin
and metalloproteinase with thrombospondin motifs 3)
(ADAM-TS 3) (ADAM-TS3) (Procollagen II amino-propeptide
processing enzyme) (Procollagen II N-proteinase) (PC
II-NP)
Length = 1205
Score = 28.5 bits (62), Expect = 2.9
Identities = 14/45 (31%), Positives = 22/45 (48%)
Query: 40 SARKMAHATFGNINLQRNPTKKANNSSKSTHPLFSFLDFRRKKKT 84
S KM H +F N + P ++ N + THPL+ ++ KT
Sbjct: 876 SDNKMVHRSFCEANKKPKPIRRMCNIQECTHPLWVAEEWEHCTKT 920
>ZBP1_RAT (Q8VDA5) Z-DNA binding protein 1 (Tumor stroma and
activated macrophage protein DLM-1)
Length = 409
Score = 28.1 bits (61), Expect = 3.7
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 10/77 (12%)
Query: 39 SSARKMAHATFGNINLQRNPTKKANNSSKSTHPLFSFLDFRRKKKTTARPEFTRYLEYLK 98
S R++ +NL+R+P + HP+FSF + TTA PE T +
Sbjct: 247 SMLRRVQLGHGNEMNLERDPVE---------HPIFSFSSSPPESTTTADPE-TAFNMQTP 296
Query: 99 EGGMWDLNSNKPVIHYK 115
E G ++H K
Sbjct: 297 EPGPHPEGGTTQIVHIK 313
>YNO3_YEAST (P53908) Hypothetical 15.3 kDa protein in MFA2-MEP2
intergenic region
Length = 130
Score = 28.1 bits (61), Expect = 3.7
Identities = 14/38 (36%), Positives = 21/38 (54%)
Query: 62 ANNSSKSTHPLFSFLDFRRKKKTTARPEFTRYLEYLKE 99
++NSSK S L ++KKK + + YL YLK+
Sbjct: 32 SSNSSKKRPKDSSLLSEKKKKKKKKKKDVLSYLSYLKD 69
>T2M3_METJA (Q58017) Type II restriction enzyme MjaIII (EC 3.1.21.4)
(Endonuclease MjaIII) (R.MjaIII)
Length = 290
Score = 28.1 bits (61), Expect = 3.7
Identities = 14/36 (38%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
Query: 76 LDFRRKKKTTARPEFTRYLEYLKEGGMWDLNSNKPV 111
L+F+ KK T E RY ++ KE G+ DL N+ +
Sbjct: 90 LEFKEKKYLTDE-EIERYYKFFKETGLEDLLKNRKI 124
>KF4A_XENLA (Q91784) Chromosome-associated kinesin KLP1
(Chromokinesin)
Length = 1226
Score = 28.1 bits (61), Expect = 3.7
Identities = 24/78 (30%), Positives = 37/78 (46%), Gaps = 8/78 (10%)
Query: 23 LQEGFKRSTSLPSRASSSARKMAHATFGNINLQRNPTKKANNSSKSTHPLFSFLDFRRKK 82
L++G S ++ S A +S +HA F I++++ NNS +S L R+K
Sbjct: 191 LEQG-NSSRTVASTAMNSQSSRSHAIF-TISIEQRKEGDKNNSFRSKLHLVDLAGSERQK 248
Query: 83 KTTARPEFTRYLEYLKEG 100
KT A + LKEG
Sbjct: 249 KTKAEG------DRLKEG 260
>SMY2_YEAST (P32909) SMY2 protein
Length = 790
Score = 27.7 bits (60), Expect = 4.9
Identities = 28/98 (28%), Positives = 41/98 (41%), Gaps = 8/98 (8%)
Query: 8 RRYERLGKETASSALLQEGFKRSTSLPSRASSSARKMAHATFGNINLQRNPTKKANNSS- 66
++ E+ K+T + EGFK S LPS SSSA A+ +N + K SS
Sbjct: 480 KKSEKTKKDTQTQT---EGFKTSKDLPSLNSSSANPAPWASKVKVNNAIETSIKNGVSST 536
Query: 67 --KSTHPLFSFLDFRRKKKTTARPEFTRYLEYLKEGGM 102
K PL L R K+ + E L + + +
Sbjct: 537 GKKKGEPL--GLQQRNSKEEKQKEELKSVLNWANKSSL 572
>VIT2_CHICK (P02845) Vitellogenin II precursor (Major vitellogenin)
[Contains: Lipovitellin I (LVI); Phosvitin (PV);
Lipovitellin II (LVII); YGP40]
Length = 1850
Score = 27.3 bits (59), Expect = 6.4
Identities = 17/65 (26%), Positives = 33/65 (50%)
Query: 4 SFTTRRYERLGKETASSALLQEGFKRSTSLPSRASSSARKMAHATFGNINLQRNPTKKAN 63
S ++R + SS+ KRS+S S +SSS+R + ++ + N + + + +
Sbjct: 1167 SSSSRSSKSSNSSKRSSSKSSNSSKRSSSSSSSSSSSSRSSSSSSSSSSNSKSSSSSSKS 1226
Query: 64 NSSKS 68
+SS S
Sbjct: 1227 SSSSS 1231
>Y285_MYCPN (P75380) Hypothetical protein MG285 homolog (F11_orf346)
Length = 346
Score = 26.9 bits (58), Expect = 8.3
Identities = 19/72 (26%), Positives = 35/72 (48%), Gaps = 3/72 (4%)
Query: 28 KRSTSLPSRASSSARKMAHATFGNINLQRNPTKKANNSSKSTHPLFSFLDFRRKKKTTAR 87
K ++ PS + K+ + + I LQ+ P KK++ +K H ++ L+ R +
Sbjct: 58 KYDSNFPSNMFQAQFKLGNWSSEKIPLQKAPDKKSDLENK-IHYFYATLEVPR--TALIK 114
Query: 88 PEFTRYLEYLKE 99
E R EY++E
Sbjct: 115 REINRISEYIRE 126
>SPEN_DROME (Q8SX83) Split ends protein
Length = 5560
Score = 26.9 bits (58), Expect = 8.3
Identities = 17/71 (23%), Positives = 35/71 (48%)
Query: 15 KETASSALLQEGFKRSTSLPSRASSSARKMAHATFGNINLQRNPTKKANNSSKSTHPLFS 74
K S+ + +E K+S + S++SSS+ K +T ++ + N +++ K + S
Sbjct: 1823 KPQQSTFVEEEHTKKSGTSTSQSSSSSSKKISSTHDKLHSKHNNRSESDKKIKKSDKNAS 1882
Query: 75 FLDFRRKKKTT 85
D R+ T+
Sbjct: 1883 SSDKRKNSSTS 1893
>OPPB_BACSU (P24138) Oligopeptide transport system permease protein
oppB
Length = 311
Score = 26.9 bits (58), Expect = 8.3
Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 3/29 (10%)
Query: 87 RPEFTRYLEYLKEGGMWDLNSNKPVIHYK 115
+P F +Y+ YLK MWD P YK
Sbjct: 57 KPLFVQYVSYLKSVAMWDFG---PSFKYK 82
>MRP2_MOUSE (Q8VI47) Canalicular multispecific organic anion
transporter 1 (ATP-binding cassette, sub-family C,
member 2)
Length = 1543
Score = 26.9 bits (58), Expect = 8.3
Identities = 22/95 (23%), Positives = 42/95 (44%), Gaps = 7/95 (7%)
Query: 11 ERLGKETASSALLQEGFKRSTSLPSRASSSARKMAHATFGNINLQRNPTKKANNSSKSTH 70
E + + AS + +E R T SR+S S + + ++ + K N +K
Sbjct: 890 EEIPDDAASLTMRRENSLRRTL--SRSSRSGSRRGKSLKSSLKI-----KSVNALNKKEE 942
Query: 71 PLFSFLDFRRKKKTTARPEFTRYLEYLKEGGMWDL 105
+ +++ T + +F+ YL+YL+ G W L
Sbjct: 943 VVKGQKLIKKEFVETGKVKFSIYLKYLQAVGWWSL 977
>KPB1_RABIT (P18688) Phosphorylase B kinase alpha regulatory chain,
skeletal muscle isoform (Phosphorylase kinase alpha M
subunit)
Length = 1237
Score = 26.9 bits (58), Expect = 8.3
Identities = 21/75 (28%), Positives = 32/75 (42%), Gaps = 3/75 (4%)
Query: 4 SFTTRRYERLGKETASSALLQEGFKRS-TSLPSRASSSARKMAHATFGNINLQRNPTKKA 62
S+ R+ G+ T + + Q TSL S ++ RKM FG +Q +
Sbjct: 548 SYLCSRWRMTGQPTITFPISQTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEF 607
Query: 63 NNSSKSTHPLFSFLD 77
+S TH SF+D
Sbjct: 608 LTTSCCTH--LSFMD 620
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.127 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,496,982
Number of Sequences: 164201
Number of extensions: 422046
Number of successful extensions: 1179
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1171
Number of HSP's gapped (non-prelim): 22
length of query: 115
length of database: 59,974,054
effective HSP length: 91
effective length of query: 24
effective length of database: 45,031,763
effective search space: 1080762312
effective search space used: 1080762312
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0212.10