BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206a.7
(300 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
EST_HEVBR (Q7Y1X1) Esterase precursor (EC 3.1.1.-) (Early nodule... 84 4e-16
APG_ARATH (P40602) Anter-specific proline-rich protein APG precu... 56 1e-07
APG_BRANA (P40603) Anter-specific proline-rich protein APG (Prot... 49 2e-05
GATB_MYCPU (Q98R09) Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransfe... 33 1.1
YJU7_YEAST (P39526) Hypothetical 229.9 kDa protein in NUC1-NCE1 ... 32 1.9
RS10_STRR6 (P66340) 30S ribosomal protein S10 31 4.3
RS10_STRPN (P66339) 30S ribosomal protein S10 31 4.3
SYM_BUCAI (P57210) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 30 5.6
YG2L_YEAST (P53254) Hypothetical 140.5 kDa protein in CTT1-PRP31... 30 9.5
>EST_HEVBR (Q7Y1X1) Esterase precursor (EC 3.1.1.-) (Early
nodule-specific protein homolog) (Latex allergen Hev b
13)
Length = 391
Score = 84.0 bits (206), Expect = 4e-16
Identities = 58/205 (28%), Positives = 94/205 (45%), Gaps = 19/205 (9%)
Query: 87 EINKNDIFIFMKNKSFTQLREIVQVVESITNTTMIALIEEGAMELGVLGNFPMGCNALIL 146
+I +ND+ N + ++ V + + + + + + GA + P+GC + IL
Sbjct: 174 DIGQNDLTEGFLNLTVEEVNATVPDLVNSFSANVKKIYDLGARTFWIHNTGPIGCLSFIL 233
Query: 147 TVVNSSTKKEDYDQFECLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYYNDVKRL 206
T + K D C +N + ++FN +LK ++ LRK A ++ D Y+ L
Sbjct: 234 TYFPWAEK----DSAGCAKAYNEVAQHFNHKLKEIVAQLRKDLPLATFVHVDIYSVKYSL 289
Query: 207 FQAPEQYGVCCIPIIACYGSSGP*NIG------------GGGKPIV--CRDLSKHINWDG 252
F PE++G P+I C G G N G K +V C S +NWDG
Sbjct: 290 FSEPEKHGFE-FPLITCCGYGGKYNFSVTAPCGDTVTADDGTKIVVGSCACPSVRVNWDG 348
Query: 253 AHFTEAAHKLIAKGLVEGPFVDPPL 277
AH+TEAA++ + G F DPP+
Sbjct: 349 AHYTEAANEYFFDQISTGAFSDPPV 373
>APG_ARATH (P40602) Anter-specific proline-rich protein APG
precursor
Length = 534
Score = 55.8 bits (133), Expect = 1e-07
Identities = 44/189 (23%), Positives = 79/189 (41%), Gaps = 25/189 (13%)
Query: 91 NDIFIFMKNKSFTQLREIVQ----VVESITNTTMIALIEEGAMELGVLGNFPMGCNALIL 146
ND+ I +L+ + ++ + ++ L GA +GV+G P+GC
Sbjct: 353 NDLIITYFGSGAQRLKNDIDSYTTIIADSAASFVLQLYGYGARRIGVIGTPPLGC----- 407
Query: 147 TVVNSSTKKEDYDQFECLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYYNDVKRL 206
V + KK+ C N + FN +L ++ L K + +Y D Y + ++
Sbjct: 408 -VPSQRLKKKKI----CNEELNYASQLFNSKLLLILGQLSKTLPNSTFVYMDIYTIISQM 462
Query: 207 FQAPEQYGV------CCIPIIACYGSSGP*NIGGGGKPIVCRDLSKHINWDGAHFTEAAH 260
+ P YG CC + G+ + +C + S ++ WDG H T+ A+
Sbjct: 463 LETPAAYGFEETKKPCCKTGLLSAGA-----LCKKSTSKICPNTSSYLFWDGVHPTQRAY 517
Query: 261 KLIAKGLVE 269
K I K L++
Sbjct: 518 KTINKVLIK 526
>APG_BRANA (P40603) Anter-specific proline-rich protein APG (Protein
CEX) (Fragment)
Length = 449
Score = 48.9 bits (115), Expect = 2e-05
Identities = 39/158 (24%), Positives = 69/158 (42%), Gaps = 25/158 (15%)
Query: 120 MIALIEEGAMELGVLGNFPMGCNALILTVVNSSTKKEDYDQFECLITHNTLIEYFNEQLK 179
++ L GA +GV+G P+GC + KK+ C N + FN +L
Sbjct: 303 VLQLYGYGARRIGVIGTPPIGCTP------SQRVKKKKI----CNEDLNYAAQLFNSKLV 352
Query: 180 HVIETLRKKFLQANIMYFDYYNDVKRLFQAPEQYGVCCIPIIACYGSSGP*NIGGGGKPI 239
++ L K + I+Y D Y+ ++ ++PE YG I C IG +
Sbjct: 353 IILGQLSKTLPNSTIVYGDIYSIFSKMLESPEDYGFEEIKKPCC-------KIGLTKGGV 405
Query: 240 VCRD--------LSKHINWDGAHFTEAAHKLIAKGLVE 269
C++ S ++ WDG H ++ A+++ + LV+
Sbjct: 406 FCKERTLKNMSNASSYLFWDGLHPSQRAYEISNRKLVK 443
>GATB_MYCPU (Q98R09) Aspartyl/glutamyl-tRNA(Asn/Gln)
amidotransferase subunit B (EC 6.3.5.-) (Asp/Glu-ADT
subunit B)
Length = 473
Score = 32.7 bits (73), Expect = 1.1
Identities = 22/55 (40%), Positives = 30/55 (54%), Gaps = 4/55 (7%)
Query: 146 LTVVNSSTKKEDYDQFECLITHNTLIEY---FNEQLKHVIETLRKKFLQANIMYF 197
L V + E DQF+ LI + +I+ FNE+ V+ET+RKK N MYF
Sbjct: 218 LNNVKKAIDLEIKDQFQKLINNQEIIQVTKRFNEK-DQVLETMRKKESTINYMYF 271
>YJU7_YEAST (P39526) Hypothetical 229.9 kDa protein in NUC1-NCE1
intergenic region
Length = 2014
Score = 32.0 bits (71), Expect = 1.9
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 7/70 (10%)
Query: 147 TVVNS-STKKEDYDQ------FECLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDY 199
TV +S ST ++D+ EC H + E +IET+ +F + I + +
Sbjct: 226 TVFDSISTDEKDFSVTFVSVLIECWTAHFKQTNFIREHSHDIIETIYSRFTEGEIGVYGF 285
Query: 200 YNDVKRLFQA 209
ND R+F A
Sbjct: 286 ANDETRIFTA 295
>RS10_STRR6 (P66340) 30S ribosomal protein S10
Length = 102
Score = 30.8 bits (68), Expect = 4.3
Identities = 30/101 (29%), Positives = 48/101 (46%), Gaps = 8/101 (7%)
Query: 97 MKNKSFTQLREIVQVVESITNTTMIALIEEGAMELG--VLGNFPMGCNALILTVVNSSTK 154
M NK ++R ++ E T T A I E A G V G P+ + T++ ++ K
Sbjct: 1 MANK---KIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPTERSLYTIIRATHK 57
Query: 155 -KEDYDQFECLITHNTLIEYFNEQLKHVIETLRKKFLQANI 194
K+ +QFE + TH LI+ N K ++ L K L + +
Sbjct: 58 YKDSREQFE-MRTHKRLIDIVNPTQK-TVDALMKLDLPSGV 96
>RS10_STRPN (P66339) 30S ribosomal protein S10
Length = 102
Score = 30.8 bits (68), Expect = 4.3
Identities = 30/101 (29%), Positives = 48/101 (46%), Gaps = 8/101 (7%)
Query: 97 MKNKSFTQLREIVQVVESITNTTMIALIEEGAMELG--VLGNFPMGCNALILTVVNSSTK 154
M NK ++R ++ E T T A I E A G V G P+ + T++ ++ K
Sbjct: 1 MANK---KIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPTERSLYTIIRATHK 57
Query: 155 -KEDYDQFECLITHNTLIEYFNEQLKHVIETLRKKFLQANI 194
K+ +QFE + TH LI+ N K ++ L K L + +
Sbjct: 58 YKDSREQFE-MRTHKRLIDIVNPTQK-TVDALMKLDLPSGV 96
>SYM_BUCAI (P57210) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 547
Score = 30.4 bits (67), Expect = 5.6
Identities = 28/107 (26%), Positives = 45/107 (41%), Gaps = 18/107 (16%)
Query: 179 KHVIETLRKKFLQANIMYFDYYNDVKRLF-------------QAPEQYGVCCIPIIACYG 225
+ + L +K L F Y+ VK++F ++ QYG C A Y
Sbjct: 108 RKIFTCLNEKGLIKEKKIFQLYDTVKKIFLPDRFIKGTCPICKSKNQYGDNCEICSATYE 167
Query: 226 SS---GP*NIGGGGKPIVCRDLSKHINWDGAHFTEAAHKLIAKGLVE 269
+ P ++ G KPI+ +KH+ +D FT K I G++E
Sbjct: 168 PTDLINPISVISGKKPILKN--TKHLYFDLPSFTNMLKKWIHSGVLE 212
>YG2L_YEAST (P53254) Hypothetical 140.5 kDa protein in CTT1-PRP31
intergenic region
Length = 1237
Score = 29.6 bits (65), Expect = 9.5
Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 10/83 (12%)
Query: 121 IALIEEGAMELGVLGNFPMGCNA-LILTVVNSSTKKEDYDQFECLITHNTLIEYFNEQLK 179
I+LI A++ G+ P G + +LT+ +K+D+ F CL + + Y
Sbjct: 183 ISLIGSFALKAGIYQ--PNGSSIDTLLTMPKELFEKKDFLNFRCLHKRSVYLAYLTH--- 237
Query: 180 HVIETLRK----KFLQANIMYFD 198
H++ L+K FLQ YFD
Sbjct: 238 HLLILLKKDKLDSFLQLEYSYFD 260
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.342 0.152 0.495
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,528,405
Number of Sequences: 164201
Number of extensions: 1248301
Number of successful extensions: 4486
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 4476
Number of HSP's gapped (non-prelim): 9
length of query: 300
length of database: 59,974,054
effective HSP length: 110
effective length of query: 190
effective length of database: 41,911,944
effective search space: 7963269360
effective search space used: 7963269360
T: 11
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0206a.7