BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206a.3
(552 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CEFD_STRCL (P18549) Isopenicillin N epimerase (EC 5.1.1.17) 94 1e-18
CEFD_NOCLA (Q03046) Isopenicillin N epimerase (EC 5.1.1.17) 86 3e-16
CSD_PYRAB (Q9V242) Probable cysteine desulfurase (EC 2.8.1.7) 63 2e-09
CSD_BACSU (O32164) Probable cysteine desulfurase (EC 2.8.1.7) 63 2e-09
CSD_MYCPA (Q9KII6) Probable cysteine desulfurase (EC 2.8.1.7) 62 5e-09
CSD_VIBCH (Q9KPQ7) Probable cysteine desulfurase (EC 2.8.1.7) 55 4e-07
CSD_BACHD (Q9K7A0) Probable cysteine desulfurase (EC 2.8.1.7) 55 4e-07
CSD_STAEP (Q8CTA4) Probable cysteine desulfurase (EC 2.8.1.7) 54 8e-07
CSD_HALN1 (Q9HMM6) Probable cysteine desulfurase (EC 2.8.1.7) 52 3e-06
CSD_METTH (O27442) Probable cysteine desulfurase (EC 2.8.1.7) 52 5e-06
CSD_XYLFA (Q9PDA6) Probable cysteine desulfurase (EC 2.8.1.7) 50 2e-05
CSD1_MYCLE (O32975) Probable cysteine desulfurase 1 (EC 2.8.1.7) 49 3e-05
CSD_STAAW (Q8NXH0) Probable cysteine desulfurase (EC 2.8.1.7) 48 6e-05
CSD_STAAN (P99177) Probable cysteine desulfurase (EC 2.8.1.7) 48 6e-05
CSD_STAAM (P63518) Probable cysteine desulfurase (EC 2.8.1.7) 48 6e-05
CSD_SYNY3 (Q55793) Probable cysteine desulfurase (EC 2.8.1.7) 47 2e-04
SUFS_PHOLL (Q7N3U5) Cysteine desulfurase (EC 2.8.1.7) (Selenocys... 46 2e-04
CSD_XYLFT (Q87DJ2) Probable cysteine desulfurase (EC 2.8.1.7) 46 2e-04
CSD2_MYCLE (Q49690) Probable cysteine desulfurase 2 (EC 2.8.1.7) 45 4e-04
YCBU_BACSU (P42253) Hypothetical protein ycbU precursor 45 5e-04
>CEFD_STRCL (P18549) Isopenicillin N epimerase (EC 5.1.1.17)
Length = 397
Score = 93.6 bits (231), Expect = 1e-18
Identities = 104/412 (25%), Positives = 159/412 (38%), Gaps = 53/412 (12%)
Query: 144 DPSVARINNGSFGCCSASVTAAQHDWQLKYLRQPDNFYFNHLKQGILRSRTIIKDLVNAD 203
DP+V +N GS G S ++ +P +F + + ++R + L+ D
Sbjct: 15 DPTVVNLNTGSGGPLPRSAFERVTGFRAHLAAEPMDFLLREVPALLWQARESLARLIGGD 74
Query: 204 HLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVKKSMEAYVTRAGG 263
L +++ N T A +V + PG+++L Y ++ E R G
Sbjct: 75 PL-RLALATNVTAAVNLVASSLRLE------APGEILLS-DDEYTPMRWCWERVARRHGL 126
Query: 264 TVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPCVVIPVKELVKIC 323
+ LP +I +A+ G + RL HV S +++P EL +
Sbjct: 127 ELRTFRLPELPSDPAEITAAAVAAM------GPRTRLFFFSHVVSTTGLILPAAELCEEA 180
Query: 324 REEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFLYSRK-------- 375
R G+ V VD AH+ G D+D+ I DFY + HKW P + FL+
Sbjct: 181 RARGITTV-VDGAHAPGFLDLDLSRIPCDFYAGSGHKWLLAPTGVGFLHLAPGRLEELEP 239
Query: 376 -------HPKGGSGSELHHPVVSHEYGNGLAVESAWI-GTRDYSAQLVVPAVIDFVNRFE 427
P GSG P +G+ + GTRD L P IDF + E
Sbjct: 240 TQVSWAYEPPEGSGP----PAARDRFGSTPGLRRLECEGTRDICPWLATPESIDF--QAE 293
Query: 428 GGIEGIKKRNHETVVEMGDMLVKAWGTHVGCP--PHMCASMVMVGLPTCLGVQSDSDALK 485
G I+ R E +L G + P P + MV LP +DA +
Sbjct: 294 LGPGAIRARRRELTDHARRLLADRPGRTLLTPDSPELSGGMVAYRLP------PGTDAAE 347
Query: 486 LRTHLREAFGVEVPIYYRPPRDGEVEPVTGYARISHQVYNKVDDYYKFRDAV 537
LR L E F +E + +PP G V RIS Y ++ + DA+
Sbjct: 348 LRRGLWERFRIEAAVAEQPP--GPV------LRISANFYTTEEEIDRLADAL 391
>CEFD_NOCLA (Q03046) Isopenicillin N epimerase (EC 5.1.1.17)
Length = 398
Score = 85.9 bits (211), Expect = 3e-16
Identities = 101/413 (24%), Positives = 161/413 (38%), Gaps = 48/413 (11%)
Query: 144 DPSVARINNGSFGCCSASVTAAQHDWQLKYLRQPDNFYFNHLKQGILRSRTIIKDLVNAD 203
DP+V +N GS G V + + + P +F L + +R + + A
Sbjct: 16 DPAVTNLNAGSCGPLPRPVFD-RVTVRARMAAGPMDFLSRQLPPLLWTARERLAGYLGA- 73
Query: 204 HLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVKKSMEAYVTRAGG 263
+ ++ N T A +V + + G +L+ Y ++ E G
Sbjct: 74 RPERLAFATNVTGAVNLVASSV-----QPHLAAGGEILLSDQEYAPMRWCWERVARHQGL 128
Query: 264 TVIEVPLPF-PVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPCVVIPVKELVKI 322
V LP P+ S D++V +A+ G + RL HV S +V+P L +
Sbjct: 129 VVRTFRLPVQPLGSPDEVVEAATAAM------GPRTRLLFFSHVVSSTGLVLPATRLCEE 182
Query: 323 CREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFLYSRK------- 375
R GV V VD A + G TD+D+ + D+Y + HKW P + FL+ +
Sbjct: 183 ARRRGVLTV-VDGAQAPGFTDLDLAALPCDYYAGSGHKWLLAPTGVGFLHFAEDQGGVLR 241
Query: 376 --------HPKGGSGSELHHPVVSHEYGNGLAVESAWIGTRDYSAQLVVPAVIDFVNRFE 427
P G + S+ + S + L E GTRD L VP+ IDF
Sbjct: 242 PPQVSWGYRPDGENPSDERNRFGSTDRLRNLECE----GTRDLCPWLAVPSAIDFQAGLG 297
Query: 428 GGIEGIKKRNHETVVEMGDMLVKAWGTHVGCPPHMCASMVMVGLPTCLGVQSDSDALKLR 487
G +++R E + L G P H S G T + +D LR
Sbjct: 298 HG--RVRERMRELAAFTRERLSGWRGLEPVTPAHPGLS----GAMTAFRLPPGTDTAGLR 351
Query: 488 THLREAFGVEVPIYYRPPRDGEVEPVTGYARISHQVYNKVDDYYKFRDAVIQL 540
L + F ++VP+ RP DG + R+S YN + + +A+ +L
Sbjct: 352 HGLWDRFRIDVPVLDRP--DGPL------LRVSTHFYNTETEVERLAEALKEL 396
>CSD_PYRAB (Q9V242) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 401
Score = 62.8 bits (151), Expect = 2e-09
Identities = 87/360 (24%), Positives = 144/360 (39%), Gaps = 40/360 (11%)
Query: 192 SRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVK 251
SR ++ D +NA+ DEI+ N + + +V E F+ GD ++ Y + +
Sbjct: 65 SRKVVADFINAE-FDEIAFTKNTSESLNLVALGL-----EHLFKKGDKIVTTPYEHHSNL 118
Query: 252 KSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPC 311
+ + G L DD E L + K +L + HV++
Sbjct: 119 LPWQRLAKKKG-------LKLEFIEGDD---EGNLDLADAEKKIKGAKLVAVQHVSNALG 168
Query: 312 VVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFL 371
V+ V+EL K+ +EEG V VDAA S+G +VD+K++ ADF + HK P I L
Sbjct: 169 VIHEVEELGKMVKEEGAIFV-VDAAQSVGHMEVDVKKLKADFLAFSGHKGPMGPTGIGVL 227
Query: 372 YSRKH-------PKGGSGSELHHPVVSHEYGNGLAVESAWIGTRDYSAQLVVPAVIDFVN 424
Y K P G G+ + ++ E GT + + + A I ++
Sbjct: 228 YINKEFFDVFEPPLIGGGTIEDVELCCYKLTE--PPERFEAGTPNIGGAIGLAAGIKYIE 285
Query: 425 RFEGGIEGIKKRNHETV---VEMGDMLVKAWGTHVGCPPHMCASMVMVGLPTCLGVQSDS 481
+ GI+ I+K+ + V E D L W H A +V +P S
Sbjct: 286 KI--GIDKIEKQERKLVKRTTEGLDELEIPWYGPRNLDKH--AGVVSFNVPPLHPHDVAS 341
Query: 482 DALKLRTHLREAFGVEVPIYYRPPRDGEVEPVTGYARISHQVYNKVDDYYKFRDAVIQLV 541
+ + +R +P+ R + G R S VYN +++ F + +LV
Sbjct: 342 VLDEHKIMVRSGHHCALPVMKR-------LKINGTVRASFHVYNSLEEVETFLGVLEELV 394
>CSD_BACSU (O32164) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 406
Score = 62.8 bits (151), Expect = 2e-09
Identities = 62/262 (23%), Positives = 112/262 (42%), Gaps = 40/262 (15%)
Query: 192 SRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVK 251
+R ++ +NA + EI TT+ +V A ++ +PGD V++ + + A
Sbjct: 70 AREKVRKFINAKSMAEIIFTKGTTTSLNMV----ALSYARANLKPGDEVVITYMEHHANI 125
Query: 252 KSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPC 311
+ V G T+ +PL S + VRE ++ ++ + HV+++
Sbjct: 126 IPWQQAVKATGATLKYIPLQEDGTISLEDVRETVTS---------NTKIVAVSHVSNVLG 176
Query: 312 VVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFC-PPSIAF 370
V P+KE+ KI + G + VD A S +D++++ DF+ + HK C P +
Sbjct: 177 TVNPIKEMAKIAHDNGA-VIVVDGAQSTPHMKIDVQDLDCDFFALSSHK--MCGPTGVGV 233
Query: 371 LYSRKHPKGGSGSELHHPVVSHEYGNGL-----AVESAW--------IGTRDYSAQLVVP 417
LY +K L + E+G + ES W GT + + +
Sbjct: 234 LYGKK--------ALLENMEPAEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLG 285
Query: 418 AVIDFVNRFEGGIEGIKKRNHE 439
A IDF+ E G++ I + H+
Sbjct: 286 AAIDFLE--EIGLDEISRHEHK 305
>CSD_MYCPA (Q9KII6) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 685
Score = 61.6 bits (148), Expect = 5e-09
Identities = 48/181 (26%), Positives = 82/181 (44%), Gaps = 15/181 (8%)
Query: 192 SRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVK 251
+R + + + A D I V T A +V AW + QPGD +++ H + A
Sbjct: 348 ARDTVAEFIGAPSSDNIVFVRGTTEAINLVAH--AWGAKH--LQPGDEIVITHLEHHANI 403
Query: 252 KSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPC 311
+ + G + P+ + + ++ EF L G + +L HV++
Sbjct: 404 VPWQLISQKTGAILKVAPID---DAGNLLLSEFEGLL------GPRTKLVAASHVSNALG 454
Query: 312 VVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFL 371
V+PV ++V++ G RV +D A SI +D+ E+GADF+ + HK + P I L
Sbjct: 455 TVMPVDKIVELGHRYGA-RVLIDGAQSIQHIPIDVAELGADFFVFSGHK-IYGPTGIGAL 512
Query: 372 Y 372
Y
Sbjct: 513 Y 513
>CSD_VIBCH (Q9KPQ7) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 404
Score = 55.5 bits (132), Expect = 4e-07
Identities = 71/346 (20%), Positives = 136/346 (38%), Gaps = 52/346 (15%)
Query: 215 TTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVKKSMEAYVTRAGGTVIEVPLPFPV 274
T A L A ++ T Q GD +L+ + A + + G V+++P+
Sbjct: 91 TRGATEALNLIAQSYARSTLQAGDEILVSETEHHANIVPWQMVAEQTGAKVVKIPM---T 147
Query: 275 KSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPCVVIPVKELVKICREEGVDRVFVD 334
+ + + FR L + ++ + H+T++ P++ +++ ++G V +D
Sbjct: 148 TTGEFGLAAFRQLLS------PRCKIVALAHITNVTGTRQPIEAVIQAAHQQGAI-VVID 200
Query: 335 AAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFLYSR------KHPKGGSGSELHHP 388
A I VD++ + ADFY + HK + P I LY + P G G +
Sbjct: 201 GAQGIVHETVDVRALDADFYVFSGHK-LYAPAGIGVLYGKTALLEAMPPWHGGGKMVEKV 259
Query: 389 VVSHEYGNGLAVESAWIGTRDYSAQLVVPAVIDFVNRF-----EGGIEGIKKRNHETVVE 443
GL + GT + + + + ID+ E + ++++ ++ + +
Sbjct: 260 SFDGTTFTGLPGKFE-AGTPNVAGAIALATAIDWYQSLDRAAVEAHLHQLQQQAYQAISQ 318
Query: 444 MGDMLVKAWGTHVGCPPHMCASMVMVGL-PTCLGVQSDSDALKLRT------HLREAFGV 496
+ D+ V + P S+VM G+ L D + +R L +AFGV
Sbjct: 319 IDDIRVLGYQ-----PNASVLSLVMDGVHHQDLATLLDQQGIAVRAGHHCAHPLMDAFGV 373
Query: 497 EVPIYYRPPRDGEVEPVTGYARISHQVYNKVDDYYKFRDAVIQLVD 542
+ G RIS VYN ++ + A+ + VD
Sbjct: 374 K-----------------GTVRISFGVYNSAEEVERLIAAIHKAVD 402
>CSD_BACHD (Q9K7A0) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 406
Score = 55.5 bits (132), Expect = 4e-07
Identities = 67/281 (23%), Positives = 117/281 (40%), Gaps = 38/281 (13%)
Query: 192 SRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVK 251
+R I+ ++A +EI TTA +V A ++ GD +++ + +
Sbjct: 70 AREKIRRFIHASSTEEIIFTRGTTTAINLV----AASYGRANLGEGDEIVITPMEHHSNI 125
Query: 252 KSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPC 311
+ G T+ +PL D ++ + K+ +K ++ I HV+++
Sbjct: 126 IPWQQVAKATGATLTYLPL-----QKDGTIK----IEDVEKTISEKTKIVAIMHVSNVLG 176
Query: 312 VVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFL 371
+ PVKE+ +I G + VD A S +D++E+G DF+ + HK P I L
Sbjct: 177 TINPVKEIAEIAHRHGAI-MLVDGAQSAPHMKIDVQELGCDFFAFSGHK-MAGPTGIGVL 234
Query: 372 YSRKHPKGGSGSELHHPVVSHEYGNGL-----AVESAWI--------GTRDYSAQLVVPA 418
Y +K + E PV E+G + +S W GT + + + A
Sbjct: 235 YGKK-----AHLEKMEPV---EFGGEMIDFVGLYDSTWKELPWKFEGGTPIIAGAIGLGA 286
Query: 419 VIDFVNRFEGGIEGIKKRNHETVVEMGDMLVKAWGTHVGCP 459
IDF+ + G++ I+K HE D L + G V P
Sbjct: 287 AIDFLE--DIGLDEIEKHEHELAQYALDRLSELEGMTVYGP 325
>CSD_STAEP (Q8CTA4) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 413
Score = 54.3 bits (129), Expect = 8e-07
Identities = 44/188 (23%), Positives = 83/188 (43%), Gaps = 15/188 (7%)
Query: 188 GILRSRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAY 247
G +R ++ +NA + +EI T + IV A ++ + GD +++ +
Sbjct: 71 GYENARETVRRFINAKYFEEIIFTRGTTASINIV----AHSYGDANISEGDEIVVTEMEH 126
Query: 248 GAVKKSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVT 307
A + R T+ +P+ K + + + ++ + K ++ I HV+
Sbjct: 127 HANIVPWQQLAKRKNATLKFIPM---TKDGELQLDDIKATIN------DKTKIVAIAHVS 177
Query: 308 SMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPS 367
++ + VK + KI E G + VD A S +DM++I ADFY+ + HK P
Sbjct: 178 NVLGTINDVKTIAKIAHEHGAV-ISVDGAQSAPHMALDMQDIDADFYSFSGHK-MLGPTG 235
Query: 368 IAFLYSRK 375
I LY ++
Sbjct: 236 IGVLYGKR 243
>CSD_HALN1 (Q9HMM6) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 415
Score = 52.4 bits (124), Expect = 3e-06
Identities = 48/177 (27%), Positives = 74/177 (41%), Gaps = 15/177 (8%)
Query: 200 VNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVKKSMEAYVT 259
V + +E+ N T A +V AW E PGD V++ + A + +
Sbjct: 87 VGGEDREEMVFTKNTTEAENLVA--FAWGLNE--LGPGDEVVLTQMEHHASLVTWQQVAD 142
Query: 260 RAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPCVVIPVKEL 319
G V +P+ +DD + +A + V H+++ V PV EL
Sbjct: 143 ETGAEVKYIPI------TDDGHLDMDAAADMITDDTALVNAV---HISNTLGTVNPVGEL 193
Query: 320 VKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFLYSRKH 376
I + G +FVD A + VD++EI ADFY + HK P I LY ++H
Sbjct: 194 ADIAHDHGA-YIFVDGAQAAPTRAVDVQEIDADFYAFSGHK-MLGPTGIGCLYGKRH 248
>CSD_METTH (O27442) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 400
Score = 51.6 bits (122), Expect = 5e-06
Identities = 47/153 (30%), Positives = 70/153 (45%), Gaps = 12/153 (7%)
Query: 299 RLAVIDHVTSMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNL 358
RL + H+++ V VKE+ +I + G VDAA SIG +VD++ IGADF
Sbjct: 156 RLVTVTHISNALGTVQDVKEIGRIAHDVGA-LYLVDAAQSIGHMEVDVRAIGADFAAFPG 214
Query: 359 HKWFFCPPSIAFLYSRKHPKG-------GSGSELHHPVVSHEYGNGLAVESAWIGTRDYS 411
HK P FLY +G G G+ L + + A A GT + +
Sbjct: 215 HKGTLGPVGTGFLYCSTDVQGELEPLMLGGGTVLDVSEDGYVLEDFPAKFEA--GTLNIA 272
Query: 412 AQLVVPAVIDFVNRFEGGIEGIKKRNHETVVEM 444
+ + A ID++NR GI I+K + E+
Sbjct: 273 GFIGLGASIDYMNRI--GIRRIQKHGMKMTEEL 303
>CSD_XYLFA (Q9PDA6) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 416
Score = 50.1 bits (118), Expect = 2e-05
Identities = 69/280 (24%), Positives = 117/280 (41%), Gaps = 26/280 (9%)
Query: 192 SRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVK 251
+RT + L+NA + +V + T AI L +WA + GDV+L+ + A
Sbjct: 83 TRTALATLLNAPTHE---LVLCSGTTFAINLIAYSWALPR--LRAGDVILVSRMEHHANI 137
Query: 252 KSMEAYVTRAGGTVIEVPLPFPVKSSD-DIVREFRSALERGKSGGKKVRLAVIDHVTSMP 310
+ R G I+V P + D D + + +V+L I HV+++
Sbjct: 138 VPWQLIAERTGAR-IQVADILPNGTLDLDALHTLMTP---------QVKLLAITHVSNVL 187
Query: 311 CVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAF 370
V P+ ++ + R+ G+ V VD + + +D+ IG DFY HK + P
Sbjct: 188 GTVNPIHDICRQARQRGITTV-VDGSQAAPHRHIDIPAIGCDFYAITGHK-LYGPTGTGA 245
Query: 371 LYSRKH-----PKGGSGSELHHPVVSHEYGNGLAVESAWIGTRDYSAQLVVPAVIDFVNR 425
L++R+ P G E+ V GT + + + + A +D+V R
Sbjct: 246 LWARREHLHIMPPFLGGGEMIKEVSFDGTLFNTPPHKFEAGTPNIAGFIGLRAAVDYVRR 305
Query: 426 FEGGIEGIKKRNHETVVEMGDMLVKAWGTHV-GCPPHMCA 464
GIE I+ R E + + + L K G + G P+ A
Sbjct: 306 I--GIEQIETRETELLAHLTEELQKIDGMRLFGTAPNKAA 343
>CSD1_MYCLE (O32975) Probable cysteine desulfurase 1 (EC 2.8.1.7)
Length = 611
Score = 49.3 bits (116), Expect = 3e-05
Identities = 45/181 (24%), Positives = 80/181 (43%), Gaps = 15/181 (8%)
Query: 192 SRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVK 251
+R + + A EI V T A +V A+A+ QPGD V++ H + A
Sbjct: 274 ARETARRFIGAAKAQEIIFVRGTTEAINLV----AYAWGGKHLQPGDEVVITHLEHHANI 329
Query: 252 KSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPC 311
+ ++ G + P+ + + ++ EF L G + +L V++
Sbjct: 330 VPWQLLSSQTGAILKVAPVD---DAGNLLMSEFEDLL------GPRTKLVAATQVSNALG 380
Query: 312 VVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFL 371
V +++V++ G RV +D A SI +++ E+GADF+ + HK + P I L
Sbjct: 381 TVTQGEKIVELGHRYGA-RVLIDGAQSIPHLPINVSELGADFFVFSGHK-IYGPTGIGVL 438
Query: 372 Y 372
Y
Sbjct: 439 Y 439
>CSD_STAAW (Q8NXH0) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 413
Score = 48.1 bits (113), Expect = 6e-05
Identities = 37/188 (19%), Positives = 84/188 (44%), Gaps = 15/188 (7%)
Query: 188 GILRSRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAY 247
G +R ++ +NA + +EI T + +V A ++ + + GD +++ +
Sbjct: 71 GYENARETVRRFINAKYFEEIIFTRGTTASINLV----AHSYGDANVEEGDEIVVTEMEH 126
Query: 248 GAVKKSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVT 307
A + R T+ +P+ + + + +++ + K ++ I H++
Sbjct: 127 HANIVPWQQLAKRKNATLKFIPMTADGELNIEDIKQ---------TINDKTKIVAIAHIS 177
Query: 308 SMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPS 367
++ + VK + +I + G + VD A + +DM+E+ ADFY+ + HK P
Sbjct: 178 NVLGTINDVKTIAEIAHQHGAI-ISVDGAQAAPHMKLDMQEMNADFYSFSGHK-MLGPTG 235
Query: 368 IAFLYSRK 375
I L+ ++
Sbjct: 236 IGVLFGKR 243
>CSD_STAAN (P99177) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 413
Score = 48.1 bits (113), Expect = 6e-05
Identities = 37/188 (19%), Positives = 84/188 (44%), Gaps = 15/188 (7%)
Query: 188 GILRSRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAY 247
G +R ++ +NA + +EI T + +V A ++ + + GD +++ +
Sbjct: 71 GYENARETVRRFINAKYFEEIIFTRGTTASINLV----AHSYGDANVEEGDEIVVTEMEH 126
Query: 248 GAVKKSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVT 307
A + R T+ +P+ + + + +++ + K ++ I H++
Sbjct: 127 HANIVPWQQLAKRKNATLKFIPMTADGELNIEDIKQ---------TINDKTKIVAIAHIS 177
Query: 308 SMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPS 367
++ + VK + +I + G + VD A + +DM+E+ ADFY+ + HK P
Sbjct: 178 NVLGTINDVKTIAEIAHQHGAI-ISVDGAQAAPHMKLDMQEMNADFYSFSGHK-MLGPTG 235
Query: 368 IAFLYSRK 375
I L+ ++
Sbjct: 236 IGVLFGKR 243
>CSD_STAAM (P63518) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 413
Score = 48.1 bits (113), Expect = 6e-05
Identities = 37/188 (19%), Positives = 84/188 (44%), Gaps = 15/188 (7%)
Query: 188 GILRSRTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAY 247
G +R ++ +NA + +EI T + +V A ++ + + GD +++ +
Sbjct: 71 GYENARETVRRFINAKYFEEIIFTRGTTASINLV----AHSYGDANVEEGDEIVVTEMEH 126
Query: 248 GAVKKSMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVT 307
A + R T+ +P+ + + + +++ + K ++ I H++
Sbjct: 127 HANIVPWQQLAKRKNATLKFIPMTADGELNIEDIKQ---------TINDKTKIVAIAHIS 177
Query: 308 SMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPS 367
++ + VK + +I + G + VD A + +DM+E+ ADFY+ + HK P
Sbjct: 178 NVLGTINDVKTIAEIAHQHGAI-ISVDGAQAAPHMKLDMQEMNADFYSFSGHK-MLGPTG 235
Query: 368 IAFLYSRK 375
I L+ ++
Sbjct: 236 IGVLFGKR 243
>CSD_SYNY3 (Q55793) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 420
Score = 46.6 bits (109), Expect = 2e-04
Identities = 59/266 (22%), Positives = 109/266 (40%), Gaps = 26/266 (9%)
Query: 193 RTIIKDLVNADHLDEISIVDNATTAAAIVLQNTAWAFREGTFQPGDVVLMLHYAYGAVKK 252
R + +NA EI NAT A +V A+++ + GD ++ + +
Sbjct: 78 RNKVAKFINARSPREIVYTRNATEAINLV----AYSWGMNNLKAGDEIITTVMEHHSNLV 133
Query: 253 SMEAYVTRAGGTVIEVPLPFPVKSSDDIVREFRSALERGKSGGKKVRLAVIDHVTSMPCV 312
+ + G + V L + S D+ F++ L +K +L + H+++
Sbjct: 134 PWQMVAAKTGAVLKFVQLD--EQESFDL-EHFKTLLS------EKTKLVTVVHISNTLGC 184
Query: 313 VIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLHKWFFCPPSIAFLY 372
V P +E+ ++ + G +V VDA S +D++ I D+ ++ HK P I FLY
Sbjct: 185 VNPAEEIAQLAHQAGA-KVLVDACQSAPHYPLDVQLIDCDWLVASGHK-MCAPTGIGFLY 242
Query: 373 SRKH-----PKGGSGSELHHPVVSHEYGNGLAVESAWIGTRDYSAQLVVPAVIDFVNRFE 427
++ P G E+ V + G GT + + + A +D++ +
Sbjct: 243 GKEEILEAMPPFFGGGEMIAEVFFDHFTTGELPHKFEAGTPAIAEAIALGAAVDYLT--D 300
Query: 428 GGIEGIKKRNHETVVEMGDMLVKAWG 453
G+E I H VE+ L + G
Sbjct: 301 LGMENI----HNYEVELTHYLWQGLG 322
>SUFS_PHOLL (Q7N3U5) Cysteine desulfurase (EC 2.8.1.7)
(Selenocysteine lyase) (EC 4.4.1.16) (Selenocysteine
reductase) (Selenocysteine beta-lyase) (SCL)
Length = 412
Score = 46.2 bits (108), Expect = 2e-04
Identities = 39/141 (27%), Positives = 68/141 (47%), Gaps = 18/141 (12%)
Query: 297 KVRLAVIDHVTSMPCVVIPVKELVKICR-----EEGVDRVFVDAAHSIGCTDVDMKEIGA 351
+ +L H++++ V PV++++K R +EG + VD A +I +D++++
Sbjct: 164 RTKLLSFTHLSNVLGTVNPVRQIIKQARKLADEQEGRLHILVDGAQAIMHKQIDVQDLDC 223
Query: 352 DFYTSNLHKWFFCPPSIAFLYSRKH------PKGGSGSELHHPVVSHEYGNGLAVESAW- 404
DFY + HK + P I LY RK P G G+ + VS + G A E W
Sbjct: 224 DFYVFSSHK-LYGPTGIGVLYGRKSLLDAMPPWEGGGAMIKQ--VSLQKGITYA-EPPWR 279
Query: 405 --IGTRDYSAQLVVPAVIDFV 423
GT + + + + A +D+V
Sbjct: 280 FEAGTPNITGIIGLGAAVDYV 300
>CSD_XYLFT (Q87DJ2) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 416
Score = 46.2 bits (108), Expect = 2e-04
Identities = 43/174 (24%), Positives = 77/174 (43%), Gaps = 10/174 (5%)
Query: 297 KVRLAVIDHVTSMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTS 356
+V+L I HV+++ V P+ ++ + R+ G+ V VD + + +++ IG DFY
Sbjct: 174 QVKLLAITHVSNVLGTVNPIHDICRQARQRGITTV-VDGSQAAPHRHINIPAIGCDFYAI 232
Query: 357 NLHKWFFCPPSIAFLYSRKH-----PKGGSGSELHHPVVSHEYGNGLAVESAWIGTRDYS 411
HK + P L++R+ P G E+ V GT + +
Sbjct: 233 TGHK-LYGPTGTGALWARREHLHIMPPFLGGGEMIKEVSFDGTLFNTPPHKFEAGTPNIA 291
Query: 412 AQLVVPAVIDFVNRFEGGIEGIKKRNHETVVEMGDMLVKAWGTHV-GCPPHMCA 464
+ + A +D+V R GI+ I+ R E + + + L K G + G P+ A
Sbjct: 292 GFIGLSAAVDYVRRI--GIDQIETRETELLAHLTEELQKIDGMRLFGTAPNKAA 343
>CSD2_MYCLE (Q49690) Probable cysteine desulfurase 2 (EC 2.8.1.7)
Length = 418
Score = 45.4 bits (106), Expect = 4e-04
Identities = 60/258 (23%), Positives = 105/258 (40%), Gaps = 26/258 (10%)
Query: 296 KKVRLAVIDHVTSMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYT 355
++V++ H +++ V PV+ELV +E G V +DA S+ VD+ +G DF
Sbjct: 171 ERVKVVAFSHHSNVTGAVAPVRELVARAKEVGALTV-LDACQSVPHQPVDLHGLGVDFAA 229
Query: 356 SNLHKWFFCPPSIAFLYSRKH-----PKGGSGSELHHPVVSHEYGNGLAVESAWIGTRDY 410
+ HK P I LY+R+ P +G + V A + GT
Sbjct: 230 FSGHK-MLGPNGIGVLYARRELLSVMPPFLTGGSMIETVTMESTTYAPAPQRFEAGTPMT 288
Query: 411 SAQLVVPAVIDFVNRFEGGIEGIKKRNHETVVEMGDMLVKAWGTHVGCPPHM-----CAS 465
S + + A +++ G++ ++ E V + L + G + P M S
Sbjct: 289 SQVVGLAAAARYLDAI--GMKAVEAHERELVAAAVEGLSRIDGVRIIGPKSMENRGSPVS 346
Query: 466 MVMVGLPT-CLGVQSDSDALKLRTHLREAFGVEVPIYYRPPRDGEVEPVTGYARISHQVY 524
V+ G+ +G D D + +R +P++ R + AR S VY
Sbjct: 347 FVVDGVHAHDIGQVLDDDGVAVRV----GHHCALPLHRR-------FALAATARASFAVY 395
Query: 525 NKVDDYYKFRDAVIQLVD 542
N VD+ + V++ +D
Sbjct: 396 NTVDEVDRLVAGVLRALD 413
>YCBU_BACSU (P42253) Hypothetical protein ycbU precursor
Length = 370
Score = 45.1 bits (105), Expect = 5e-04
Identities = 26/76 (34%), Positives = 38/76 (49%), Gaps = 1/76 (1%)
Query: 300 LAVIDHVTSMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYTSNLH 359
L + HV V +K + +I + +G +FVDA S G +D+KE G D +
Sbjct: 148 LTCVPHVHYRDGYVQDIKAIAEISQRKG-SLLFVDAYQSAGHIPIDVKEWGVDMLAAGTR 206
Query: 360 KWFFCPPSIAFLYSRK 375
K+ P +AFLY RK
Sbjct: 207 KYLLGIPGVAFLYVRK 222
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.137 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,112,871
Number of Sequences: 164201
Number of extensions: 2949023
Number of successful extensions: 8058
Number of sequences better than 10.0: 111
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 95
Number of HSP's that attempted gapping in prelim test: 7974
Number of HSP's gapped (non-prelim): 134
length of query: 552
length of database: 59,974,054
effective HSP length: 115
effective length of query: 437
effective length of database: 41,090,939
effective search space: 17956740343
effective search space used: 17956740343
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0206a.3