BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0203.9
(529 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CBP8_MOUSE (Q9DBB9) Carboxypeptidase N 83 kDa chain precursor (C... 39 0.033
GLB2_NIPBR (P51535) Myoglobin (Globin, body wall isoform) 36 0.28
ESA8_TRYBB (P23799) Putative adenylate cyclase regulatory protei... 35 0.37
YA84_MYCPN (P75609) Hypothetical lipoprotein MPN084 precursor (R... 34 0.82
ASPM_COLGU (P62287) Abnormal spindle-like microcephaly-associate... 34 1.1
NU2M_CHICK (P18937) NADH-ubiquinone oxidoreductase chain 2 (EC 1... 33 2.4
KPYK_EMENI (P22360) Pyruvate kinase (EC 2.7.1.40) (PK) 33 2.4
YN96_YEAST (P53753) Hypothetical 121.1 kDa protein in BIO3-HXT17... 32 4.1
TUAG_BACSU (O32268) Putative teichuronic acid biosynthesis glyco... 32 4.1
PARG_HUMAN (Q86W56) Poly(ADP-ribose) glycohydrolase (EC 3.2.1.143) 32 4.1
IRA4_HUMAN (Q9NWZ3) Interleukin-1 receptor-associated kinase-4 (... 32 4.1
INX3_CAEEL (Q19746) Innexin inx-3 32 4.1
ILVC_BUCSC (Q9RQ51) Ketol-acid reductoisomerase (EC 1.1.1.86) (A... 32 4.1
ASPM_MACMU (P62292) Abnormal spindle-like microcephaly-associate... 32 4.1
GLT5_RAT (O88422) Polypeptide N-acetylgalactosaminyltransferase ... 32 5.3
GLT5_HUMAN (Q7Z7M9) Polypeptide N-acetylgalactosaminyltransferas... 32 5.3
NU2M_COTJA (P24971) NADH-ubiquinone oxidoreductase chain 2 (EC 1... 31 7.0
MURD_WIGBR (Q8D2Z4) UDP-N-acetylmuramoylalanine--D-glutamate lig... 31 7.0
KPYK_ASPNG (Q12669) Pyruvate kinase (EC 2.7.1.40) (PK) 31 7.0
FXLC_MOUSE (Q9EPX5) F-box/LRR-repeat protein 12 (F-box and leuci... 31 7.0
>CBP8_MOUSE (Q9DBB9) Carboxypeptidase N 83 kDa chain precursor
(Carboxypeptidase N regulatory subunit)
(Carboxypeptidase N polypeptide 2)
Length = 547
Score = 38.9 bits (89), Expect = 0.033
Identities = 74/326 (22%), Positives = 126/326 (37%), Gaps = 52/326 (15%)
Query: 25 DRISDLPDAVLHHILSLLPIKTA---AQTSILSKRWKFLWTTFPDLDFRTIDPHAFSSRN 81
++++D+P + HI ++ ++TA +T S F + R ++P AF
Sbjct: 38 EQLADIPPDIPPHITDIVFVETAFTTVRTRAFSGSPNLTKVVFLNTQVRHLEPDAFGGLP 97
Query: 82 LQSSDFETSGHPLASTMDFITQVLSTRDKDSDIRALCFRARLSFSRLNSLIRSAVRHNVK 141
+ D E +G P+++ I LS+ +K L F RL L H
Sbjct: 98 -RLQDLEITGSPVSNLSAHIFSNLSSLEK----------LTLDFDRLAGLPEDLFCH--- 143
Query: 142 ELDIEVTPEVRTDEYFNFPRCLIAS-KTLRVLKLRSGFRLPPSSIMKETFQSLHTLS--- 197
+DI + +++ ++ P L S + LR L L + + K FQSL L
Sbjct: 144 -MDILESLQLQGNQLRTLPGRLFQSLRDLRTLNLAQNLL---TQLPKGAFQSLTGLQMLK 199
Query: 198 --------LTLGPVDHQAPLSDLFTE-SAFPFLRNLHLQLCFGLRVLRVGCLALRDLSLE 248
L G + + L +LF + +A L F L +L + A+ L +
Sbjct: 200 LSNNMLARLPEGALGSLSSLQELFLDGNAITELSPHLFSQLFSLEMLWLQHNAICHLPVS 259
Query: 249 KCINLHGLDVVTPKLERLRVSECFHGYHNKSWVRI----------------NAPKLESLF 292
+LH L ++ K LR HN+ + + N +L SL
Sbjct: 260 LFSSLHNLTFLSLKDNALRTLPEGLFAHNQGLLHLSLSYNQLETIPEGAFTNLSRLVSLT 319
Query: 293 WQYNAVADVT--VFERSNVLHEASVD 316
+NA+ D+ VF L + S+D
Sbjct: 320 LSHNAITDLPEHVFRNLEQLVKLSLD 345
>GLB2_NIPBR (P51535) Myoglobin (Globin, body wall isoform)
Length = 154
Score = 35.8 bits (81), Expect = 0.28
Identities = 26/81 (32%), Positives = 38/81 (46%), Gaps = 4/81 (4%)
Query: 39 LSLLPIKTAAQTSILSKRWKFLWTTFPDLDFRTIDPHAFSSRNLQSSD-FETSGHPLAST 97
LSL PI A Q + +KF +T PDL F++ ++Q SD FE G L +
Sbjct: 12 LSLAPISKAQQAQVGKDFYKFFFTNHPDLRKYFKGAENFTADDVQKSDRFEKLGSGLLLS 71
Query: 98 MDFITQVLSTRDKDSDIRALC 118
+ + +T D + RA C
Sbjct: 72 VHILA---NTFDNEDVFRAFC 89
>ESA8_TRYBB (P23799) Putative adenylate cyclase regulatory protein
(Leucine repeat protein) (VSG expression site-associated
protein F14.9)
Length = 630
Score = 35.4 bits (80), Expect = 0.37
Identities = 48/194 (24%), Positives = 83/194 (42%), Gaps = 14/194 (7%)
Query: 218 FPFLRNLHLQLCF--GLRVLRVGCLALRDLSLEKCIN---LHGLDVVTPKLERLRVSECF 272
F LR L + C G V+ + L+ LS+ C N L+GL+ + LE+L +S C
Sbjct: 300 FSNLRELDISGCLVLGSAVVLKNLINLKVLSVSNCKNFKDLNGLERLV-NLEKLNLSGC- 357
Query: 273 HGYHNKSWVRINAPKLESLFWQYNAVADVTVFERSNVLHEASVDFVLVMSKNDKVSMDKL 332
HG + +V N L+ L + + F+ L+ V ++ + V K
Sbjct: 358 HGVSSLGFVA-NLSNLKEL--DISGCESLVCFDGLQDLNNLEVLYLRDVKSFTNVGAIKN 414
Query: 333 QSAINFMAGLSCARSLTLDSKTIELQKRITSMCSSPWLQMLSNSNFYIEPLYNLKSLELH 392
S + + C R +L +E K + + +++S + LY+L+ L +
Sbjct: 415 LSKMRELDLSGCERITSLSG--LETLKGLEELSLEGCGEIMSFDPIW--SLYHLRVLYVS 470
Query: 393 TCFNKTNIQGLACL 406
C N ++ GL CL
Sbjct: 471 ECGNLEDLSGLQCL 484
>YA84_MYCPN (P75609) Hypothetical lipoprotein MPN084 precursor
(R02_orf524)
Length = 524
Score = 34.3 bits (77), Expect = 0.82
Identities = 22/95 (23%), Positives = 39/95 (40%)
Query: 429 KQWNKDLDMTSNEEEQYWESEIPTLKPFLQHLKVVEIHGFLDCENEVTLAKFLLKNGKAL 488
KQWNK D +EQ + + KP V+E+ FLD + + + +K
Sbjct: 179 KQWNKKSDQKDQTQEQTVIGQSWSYKPAYADFAVIEVPLFLDNVRDKQVFDYFVKPAIET 238
Query: 489 EEMVYFTGHCNTSSTLRRQKVRQHMLMGVSWASSN 523
+ + T T L+ + ++++G SN
Sbjct: 239 YKKLGDTAQLFTEQNLKELEKETYIMLGYPVVESN 273
>ASPM_COLGU (P62287) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3477
Score = 33.9 bits (76), Expect = 1.1
Identities = 32/144 (22%), Positives = 62/144 (42%), Gaps = 11/144 (7%)
Query: 254 HGLDVVTPKLERLRVSECFHGYHNKSWVRINAPKLESLFWQYNAVADVTVFERSNVLHEA 313
HG + + R H N+ + ++ + + + AVA+ T F NV ++
Sbjct: 232 HGATCLPLSVRRSTTYSSLHASENRELLNVDNTNVSKVSFNEKAVAETT-FNSMNVSGQS 290
Query: 314 SVDFVLVMSKNDKVSMDKLQSAINFMAGLSCARSLTLDSKTIELQKRITSMCSSPWLQML 373
+ L+++ N +++ QS INF++ S + + +EL C S + M
Sbjct: 291 GENSKLILTPNYSSTLNVTQSQINFLSPDSFVNNSHGANNELEL-----VTCLSSDMFMT 345
Query: 374 SNSNFYIEPLYNLKSLELHTCFNK 397
NS +P++ L+S H + K
Sbjct: 346 DNS----KPVH-LQSTTAHEIYQK 364
>NU2M_CHICK (P18937) NADH-ubiquinone oxidoreductase chain 2 (EC
1.6.5.3)
Length = 346
Score = 32.7 bits (73), Expect = 2.4
Identities = 18/45 (40%), Positives = 26/45 (57%)
Query: 156 YFNFPRCLIASKTLRVLKLRSGFRLPPSSIMKETFQSLHTLSLTL 200
+F FP L S + L L + +LPP +++ T QSL+T LTL
Sbjct: 112 HFWFPEVLQGSSLITALLLSTLMKLPPITLLLLTSQSLNTTLLTL 156
>KPYK_EMENI (P22360) Pyruvate kinase (EC 2.7.1.40) (PK)
Length = 526
Score = 32.7 bits (73), Expect = 2.4
Identities = 18/46 (39%), Positives = 24/46 (52%)
Query: 233 RVLRVGCLALRDLSLEKCINLHGLDVVTPKLERLRVSECFHGYHNK 278
+ LRV CL ++S K +NL G DV P L +S+ G NK
Sbjct: 175 KTLRVRCLNNGNISSRKGVNLPGTDVDLPALSEKDISDLKFGVKNK 220
>YN96_YEAST (P53753) Hypothetical 121.1 kDa protein in BIO3-HXT17
intergenic region precursor
Length = 1117
Score = 32.0 bits (71), Expect = 4.1
Identities = 25/99 (25%), Positives = 42/99 (42%)
Query: 78 SSRNLQSSDFETSGHPLASTMDFITQVLSTRDKDSDIRALCFRARLSFSRLNSLIRSAVR 137
S NL S S P ASTM +T +LS S +S ++LN + ++V
Sbjct: 135 SVHNLSSVSGVASVMPSASTMRKVTTLLSQTASTSTSTLFSSSLSISGTQLNGTLLTSVS 194
Query: 138 HNVKELDIEVTPEVRTDEYFNFPRCLIASKTLRVLKLRS 176
+ + P + E P L ++KT+ + +R+
Sbjct: 195 KGTIDPLVTQMPSYSSQETKIIPSSLTSNKTIYTISVRT 233
>TUAG_BACSU (O32268) Putative teichuronic acid biosynthesis glycosyl
transferase tuaG (EC 2.4.1.-)
Length = 252
Score = 32.0 bits (71), Expect = 4.1
Identities = 16/44 (36%), Positives = 22/44 (49%)
Query: 306 RSNVLHEASVDFVLVMSKNDKVSMDKLQSAINFMAGLSCARSLT 349
R+ L A +V + +DK DKL+ + FM SCA S T
Sbjct: 78 RNKALERAQGRYVAFLDSDDKWKKDKLEKQLEFMMERSCAFSFT 121
>PARG_HUMAN (Q86W56) Poly(ADP-ribose) glycohydrolase (EC 3.2.1.143)
Length = 976
Score = 32.0 bits (71), Expect = 4.1
Identities = 38/135 (28%), Positives = 53/135 (39%), Gaps = 27/135 (20%)
Query: 341 GLSCARSLTLDSKTIELQKRITSMCSSPWLQMLSNSNFY---IEPLYNLKSLELHTCFNK 397
G+ A S +LDSK RI SM SS + NFY +E L N+ L L +
Sbjct: 87 GIKTAESESLDSKENN-NTRIESMMSS-----VQKDNFYQHNVEKLENVSQLSLDKSPTE 140
Query: 398 TNIQGL-------AC-----------LFRSSPTLHTLIVKIIDHNKFDRKQWNKDLDMTS 439
+ Q L C L S P TL+ + + DR N D T
Sbjct: 141 KSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTD 200
Query: 440 NEEEQYWESEIPTLK 454
+EE + + + T+K
Sbjct: 201 SEENRDNQQFLTTVK 215
>IRA4_HUMAN (Q9NWZ3) Interleukin-1 receptor-associated kinase-4 (EC
2.7.1.37) (IRAK-4) (NY-REN-64 antigen)
Length = 460
Score = 32.0 bits (71), Expect = 4.1
Identities = 23/89 (25%), Positives = 43/89 (47%), Gaps = 2/89 (2%)
Query: 435 LDMTSNEEEQYWESEIPTLKPFLQHLKVVEIHGFLDCENEVTLAKFLLKNGKALEEMVYF 494
+D+T+ E +Q ++ EI + QH +VE+ GF +++ L + NG L+ +
Sbjct: 219 VDITTEELKQQFDQEIKVMAK-CQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCL 277
Query: 495 TGHCNTSSTLRRQKVRQHMLMGVSWASSN 523
G S + R K+ Q G+++ N
Sbjct: 278 DGTPPLSWHM-RCKIAQGAANGINFLHEN 305
>INX3_CAEEL (Q19746) Innexin inx-3
Length = 420
Score = 32.0 bits (71), Expect = 4.1
Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Query: 67 LDFRTIDPHAFSSRNLQSSDFETSGHPLASTMDFITQVLSTRDKDSDIRALCFRARLSFS 126
LDF ++ A + L+S D ET + +DFI +L TR K+ R C+R
Sbjct: 132 LDFPSVISEAEA---LRSQDSETRTKGVNKLVDFIGDILDTRSKNEYGRFYCYRFGKGLG 188
Query: 127 RLNSLIRSAVR 137
+ S++ ++
Sbjct: 189 SMTSMLYICIK 199
>ILVC_BUCSC (Q9RQ51) Ketol-acid reductoisomerase (EC 1.1.1.86)
(Acetohydroxy-acid isomeroreductase)
(Alpha-keto-beta-hydroxylacil reductoisomerase)
Length = 489
Score = 32.0 bits (71), Expect = 4.1
Identities = 41/160 (25%), Positives = 66/160 (40%), Gaps = 36/160 (22%)
Query: 137 RHNVKELDIEVTP----EVRTDEYFNFPRCLIASKTLRVLKLRSGFRLPPSSIMKET--- 189
R +K+ D E E+ EYF+ L V L++G L IM ET
Sbjct: 330 RTQIKKTDFEKCKICHKEISEQEYFD-------QGLLMVAILKAGIELS-FEIMVETGIK 381
Query: 190 -----FQSLHTLSLTLGPV------DHQAPLSD-------LFTESAFPFLRNLHLQLCFG 231
++SLH L L + + +SD LF++SA P L+ +LC G
Sbjct: 382 EESAYYESLHELPLIANTIARKRLYEMNLVISDTAEYGSYLFSQSAIPLLKEFMNELCPG 441
Query: 232 LRVLRVGCLALRDLSLEKC---INLHGLDVVTPKLERLRV 268
++ L +++L K I H ++++ KL + V
Sbjct: 442 DLGEKISDLQFDNVTLNKINHDIENHPVEIIGKKLRKYMV 481
>ASPM_MACMU (P62292) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3479
Score = 32.0 bits (71), Expect = 4.1
Identities = 31/144 (21%), Positives = 62/144 (42%), Gaps = 11/144 (7%)
Query: 254 HGLDVVTPKLERLRVSECFHGYHNKSWVRINAPKLESLFWQYNAVADVTVFERSNVLHEA 313
HG + + R H N+ + +++ + + + AV + T F NV ++
Sbjct: 232 HGATCLPLSVRRSTTYSSLHASENRELLNVDSANVSKVSFNEKAVTE-TSFNSINVNDQS 290
Query: 314 SVDFVLVMSKNDKVSMDKLQSAINFMAGLSCARSLTLDSKTIELQKRITSMCSSPWLQML 373
+ L+++ N +++ QS INF++ S + + +EL C S + M
Sbjct: 291 GENSKLILTPNYSSTLNITQSQINFLSPDSFVNNSHGANNELEL-----VTCLSSDMFMT 345
Query: 374 SNSNFYIEPLYNLKSLELHTCFNK 397
NS +P++ L+S H + K
Sbjct: 346 DNS----KPVH-LQSTTAHEIYQK 364
>GLT5_RAT (O88422) Polypeptide N-acetylgalactosaminyltransferase 5
(EC 2.4.1.41) (Protein-UDP
acetylgalactosaminyltransferase 5)
(UDP-GalNAc:polypeptide
N-acetylgalactosaminyltransferase 5) (Polypeptide GalNAc
transferase 5) (GalNAc-T5) (pp-GaNTase 5)
Length = 930
Score = 31.6 bits (70), Expect = 5.3
Identities = 17/62 (27%), Positives = 33/62 (52%), Gaps = 5/62 (8%)
Query: 369 WLQMLSNSNFYIEPLYNLKSLELHTCFNKTNIQGLACLFRSSPTLHTLIVKIIDHNKFDR 428
W++++ + + + P+ + SL L+ C N+ N L L RS+ H +++DH F+
Sbjct: 838 WVRLIKHGEWCVAPIPDKGSLTLYPCDNRNN--RLKWLHRSASAFHP---ELVDHIVFES 892
Query: 429 KQ 430
Q
Sbjct: 893 YQ 894
>GLT5_HUMAN (Q7Z7M9) Polypeptide N-acetylgalactosaminyltransferase 5
(EC 2.4.1.41) (Protein-UDP
acetylgalactosaminyltransferase 5)
(UDP-GalNAc:polypeptide
N-acetylgalactosaminyltransferase 5) (Polypeptide GalNAc
transferase 5) (GalNAc-T5) (pp-GaNTase 5)
Length = 940
Score = 31.6 bits (70), Expect = 5.3
Identities = 19/100 (19%), Positives = 49/100 (49%), Gaps = 9/100 (9%)
Query: 335 AINFMAGLSCARSLTLDSKTIELQ----KRITSMCSSPWLQMLSNSNFYIEPLYNLKSLE 390
A + ++ + +++++ T+ L+ + + WL+++ + I P+ + ++
Sbjct: 810 ASGVLINVALGKCISIENTTVILEDCDGSKELQQFNYTWLRLIKCGEWCIAPIPDKGAVR 869
Query: 391 LHTCFNKTNIQGLACLFRSSPTLHTLIVKIIDHNKFDRKQ 430
LH C N+ +GL L +S+ H ++++H F+ Q
Sbjct: 870 LHPCDNRN--KGLKWLHKSTSVFHP---ELVNHIVFENNQ 904
>NU2M_COTJA (P24971) NADH-ubiquinone oxidoreductase chain 2 (EC
1.6.5.3)
Length = 346
Score = 31.2 bits (69), Expect = 7.0
Identities = 17/45 (37%), Positives = 25/45 (54%)
Query: 156 YFNFPRCLIASKTLRVLKLRSGFRLPPSSIMKETFQSLHTLSLTL 200
+F FP L S + L L + +LPP +++ T QSL+ LTL
Sbjct: 112 HFWFPEVLQGSSLITALLLSTLMKLPPMTLLLMTSQSLNPALLTL 156
>MURD_WIGBR (Q8D2Z4) UDP-N-acetylmuramoylalanine--D-glutamate ligase
(EC 6.3.2.9) (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
synthetase) (D-glutamic acid adding enzyme)
Length = 438
Score = 31.2 bits (69), Expect = 7.0
Identities = 23/92 (25%), Positives = 42/92 (45%), Gaps = 10/92 (10%)
Query: 298 VADVTVFERSNVLHEASVDFVLVMSKNDKVSMDKLQSAINFMAGLSCARSLTLDSKTIEL 357
+ D+ +F R EAS + + N K ++ + AI +GLS L + ++L
Sbjct: 93 IGDIELFAR-----EASRPIIAITGSNGKSTVTSMIGAIALNSGLSVGIGGNLGTPALDL 147
Query: 358 QKRITSMCSSPWLQMLSNSNFYIEPLYNLKSL 389
S L ++ S+F +E +NLK++
Sbjct: 148 LNE-----KSYQLYIIELSSFQLETTFNLKTI 174
>KPYK_ASPNG (Q12669) Pyruvate kinase (EC 2.7.1.40) (PK)
Length = 526
Score = 31.2 bits (69), Expect = 7.0
Identities = 16/46 (34%), Positives = 24/46 (51%)
Query: 233 RVLRVGCLALRDLSLEKCINLHGLDVVTPKLERLRVSECFHGYHNK 278
+ +RV CL ++S K +NL G DV P L +++ G NK
Sbjct: 175 KTIRVRCLNNGNISSRKGVNLPGTDVDLPALSEKDIADLKFGVRNK 220
>FXLC_MOUSE (Q9EPX5) F-box/LRR-repeat protein 12 (F-box and
leucine-rich repeat protein 12) (F-box protein FBL12)
Length = 326
Score = 31.2 bits (69), Expect = 7.0
Identities = 15/32 (46%), Positives = 19/32 (58%)
Query: 29 DLPDAVLHHILSLLPIKTAAQTSILSKRWKFL 60
DLPD VL I S LP++ + S + RWK L
Sbjct: 6 DLPDLVLLEIFSYLPVRDRIRISRVCHRWKRL 37
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.134 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,640,574
Number of Sequences: 164201
Number of extensions: 2208437
Number of successful extensions: 5236
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 5222
Number of HSP's gapped (non-prelim): 26
length of query: 529
length of database: 59,974,054
effective HSP length: 115
effective length of query: 414
effective length of database: 41,090,939
effective search space: 17011648746
effective search space used: 17011648746
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0203.9